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Transient responses of double-curved sandwich two-layer shells resting on Kerr's foundations with laminated three-phase polymer/GNP/fiber surface and auxetic honeycomb core subjected to the blast load
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作者 Nguyen Thi Hai Van Thi Hong Nguyen 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第5期222-247,共26页
This work uses refined first-order shear theory to analyze the free vibration and transient responses of double-curved sandwich two-layer shells made of auxetic honeycomb core and laminated three-phase polymer/GNP/fib... This work uses refined first-order shear theory to analyze the free vibration and transient responses of double-curved sandwich two-layer shells made of auxetic honeycomb core and laminated three-phase polymer/GNP/fiber surface subjected to the blast load.Each of the two layers that make up the double-curved shell structure is made up of an auxetic honeycomb core and two laminated sheets of three-phase polymer/GNP/fiber.The exterior is supported by a Kerr elastic foundation with three characteristics.The key innovation of the proposed theory is that the transverse shear stresses are zero at two free surfaces of each layer.In contrast to previous first-order shear deformation theories,no shear correction factor is required.Navier's exact solution was used to treat the double-curved shell problem with a single title boundary,while the finite element technique and an eight-node quadrilateral were used to address the other boundary requirements.To ensure the accuracy of these results,a thorough comparison technique is employed in conjunction with credible statements.The problem model's edge cases allow for this kind of analysis.The study's findings may be used in the post-construction evaluation of military and civil works structures for their ability to sustain explosive loads.In addition,this is also an important basis for the calculation and design of shell structures made of smart materials when subjected to shock waves or explosive loads. 展开更多
关键词 Blast load two-layer shell Polymer/GNP/Fiber surface Auxetic honeycomb Shear connectors
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A Two-Layer Encoding Learning Swarm Optimizer Based on Frequent Itemsets for Sparse Large-Scale Multi-Objective Optimization
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作者 Sheng Qi Rui Wang +3 位作者 Tao Zhang Xu Yang Ruiqing Sun Ling Wang 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第6期1342-1357,共16页
Traditional large-scale multi-objective optimization algorithms(LSMOEAs)encounter difficulties when dealing with sparse large-scale multi-objective optimization problems(SLM-OPs)where most decision variables are zero.... Traditional large-scale multi-objective optimization algorithms(LSMOEAs)encounter difficulties when dealing with sparse large-scale multi-objective optimization problems(SLM-OPs)where most decision variables are zero.As a result,many algorithms use a two-layer encoding approach to optimize binary variable Mask and real variable Dec separately.Nevertheless,existing optimizers often focus on locating non-zero variable posi-tions to optimize the binary variables Mask.However,approxi-mating the sparse distribution of real Pareto optimal solutions does not necessarily mean that the objective function is optimized.In data mining,it is common to mine frequent itemsets appear-ing together in a dataset to reveal the correlation between data.Inspired by this,we propose a novel two-layer encoding learning swarm optimizer based on frequent itemsets(TELSO)to address these SLMOPs.TELSO mined the frequent terms of multiple particles with better target values to find mask combinations that can obtain better objective values for fast convergence.Experi-mental results on five real-world problems and eight benchmark sets demonstrate that TELSO outperforms existing state-of-the-art sparse large-scale multi-objective evolutionary algorithms(SLMOEAs)in terms of performance and convergence speed. 展开更多
关键词 Evolutionary algorithms learning swarm optimiza-tion sparse large-scale optimization sparse large-scale multi-objec-tive problems two-layer encoding.
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Two-Layer Attention Feature Pyramid Network for Small Object Detection
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作者 Sheng Xiang Junhao Ma +2 位作者 Qunli Shang Xianbao Wang Defu Chen 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第10期713-731,共19页
Effective small object detection is crucial in various applications including urban intelligent transportation and pedestrian detection.However,small objects are difficult to detect accurately because they contain les... Effective small object detection is crucial in various applications including urban intelligent transportation and pedestrian detection.However,small objects are difficult to detect accurately because they contain less information.Many current methods,particularly those based on Feature Pyramid Network(FPN),address this challenge by leveraging multi-scale feature fusion.However,existing FPN-based methods often suffer from inadequate feature fusion due to varying resolutions across different layers,leading to suboptimal small object detection.To address this problem,we propose the Two-layerAttention Feature Pyramid Network(TA-FPN),featuring two key modules:the Two-layer Attention Module(TAM)and the Small Object Detail Enhancement Module(SODEM).TAM uses the attention module to make the network more focused on the semantic information of the object and fuse it to the lower layer,so that each layer contains similar semantic information,to alleviate the problem of small object information being submerged due to semantic gaps between different layers.At the same time,SODEM is introduced to strengthen the local features of the object,suppress background noise,enhance the information details of the small object,and fuse the enhanced features to other feature layers to ensure that each layer is rich in small object information,to improve small object detection accuracy.Our extensive experiments on challenging datasets such as Microsoft Common Objects inContext(MSCOCO)and Pattern Analysis Statistical Modelling and Computational Learning,Visual Object Classes(PASCAL VOC)demonstrate the validity of the proposedmethod.Experimental results show a significant improvement in small object detection accuracy compared to state-of-theart detectors. 展开更多
关键词 Small object detection two-layer attention module small object detail enhancement module feature pyramid network
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Two-layer formation-containment fault-tolerant control of fixed-wing UAV swarm for dynamic target tracking 被引量:1
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作者 QIN Boyu ZHANG Dong +1 位作者 TANG Shuo XU Yang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第6期1375-1396,共22页
This paper tackles the formation-containment control problem of fixed-wing unmanned aerial vehicle(UAV)swarm with model uncertainties for dynamic target tracking in three-dimensional space in the faulty case of UAVs’... This paper tackles the formation-containment control problem of fixed-wing unmanned aerial vehicle(UAV)swarm with model uncertainties for dynamic target tracking in three-dimensional space in the faulty case of UAVs’actuator and sensor.The fixed-wing UAV swarm under consideration is organized as a“multi-leader-multi-follower”structure,in which only several leaders can obtain the dynamic target information while others only receive the neighbors’information through the communication network.To simultaneously realize the formation,containment,and dynamic target tracking,a two-layer control framework is adopted to decouple the problem into two subproblems:reference trajectory generation and trajectory tracking.In the upper layer,a distributed finite-time estimator(DFTE)is proposed to generate each UAV’s reference trajectory in accordance with the control objective.Subsequently,a distributed composite robust fault-tolerant trajectory tracking controller is developed in the lower layer,where a novel adaptive extended super-twisting(AESTW)algorithm with a finite-time extended state observer(FTESO)is involved in solving the robust trajectory tracking control problem under model uncertainties,actuator,and sensor faults.The proposed controller simultaneously guarantees rapidness and enhances the system’s robustness with fewer chattering effects.Finally,corresponding simulations are carried out to demonstrate the effectiveness and competitiveness of the proposed two-layer fault-tolerant cooperative control scheme. 展开更多
关键词 fixed-wing unmanned aerial vehicle(UAV)swarm two-layer control formation-containment dynamic target tracking
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A Two-Layer Fuzzy Control Strategy for the Participation of Energy Storage Battery Systems in Grid Frequency Regulation 被引量:1
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作者 Wei Chen Na Sun +2 位作者 Zhicheng Ma Wenfei Liu Haiying Dong 《Energy Engineering》 EI 2023年第6期1445-1464,共20页
To address the frequency fluctuation problem caused by the power dynamic imbalance between the power system and the loadwhen a large number of newenergy sources are connected to the grid,a two-layer fuzzy control stra... To address the frequency fluctuation problem caused by the power dynamic imbalance between the power system and the loadwhen a large number of newenergy sources are connected to the grid,a two-layer fuzzy control strategy is proposed for the participation of the energy storage battery system in FM.Firstly,considering the coordination of FM units responding to automatic power generation control commands,a comprehensive allocation strategy of two signals under automatic power generation control commands is proposed to give full play to the advantages of two FM signals while enabling better coordination of two FM units responding to FM commands;secondly,based on the grid FM demand and battery FM capability,a double-layer fuzzy control strategy is proposed for FM units responding to automatic power generation control commands in a coordinated manner under dual-signal allocation mode to precisely allocate the power output depth of FM units,which can control the fluctuation of frequency deviation within a smaller range at a faster speed while maintaining the battery charge state;finally,the proposed Finally,the proposed control strategy is simulated and verified inMatlab/Simulink.The results show that the proposed control strategy can control the frequency deviation within a smaller range in a shorter time,better stabilize the fluctuation of the battery charge level,and improve the utilization of the FM unit. 展开更多
关键词 Battery energy storage secondary FM signal distribution mode charge state two-layer fuzzy control
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Dynamic simulation analysis of molten salt reactor-coupled air-steam combined cycle power generation system 被引量:2
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作者 Jing-Lei Huang Guo-Bin Jia +3 位作者 Li-Feng Han Wen-Qian Liu Li Huang Zheng-Han Yang 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2024年第2期222-233,共12页
A nonlinear dynamic simulation model based on coordinated control of speed and flow rate for the molten salt reactor and combined cycle systems is proposed here to ensure the coordination and stability between the mol... A nonlinear dynamic simulation model based on coordinated control of speed and flow rate for the molten salt reactor and combined cycle systems is proposed here to ensure the coordination and stability between the molten salt reactor and power system.This model considers the impact of thermal properties of fluid variation on accuracy and has been validated with Simulink.This study reveals the capability of the control system to compensate for anomalous situations and maintain shaft stability in the event of perturbations occurring in high-temperature molten salt tank outlet parameters.Meanwhile,the control system’s impact on the system’s dynamic characteristics under molten salt disturbance is also analyzed.The results reveal that after the disturbance occurs,the controlled system benefits from the action of the control,and the overshoot and disturbance amplitude are positively correlated,while the system power and frequency eventually return to the initial values.This simulation model provides a basis for utilizing molten salt reactors for power generation and maintaining grid stability. 展开更多
关键词 Molten salt reactor Combined cycle Dynamic characteristic CONTROL
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Failure transition of shear-to-dilation band of rock salt under triaxial stresses 被引量:3
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作者 Jianfeng Liu Xiaosong Qiu +3 位作者 Jianxiong Yang Chao Liang Jingjing Dai Yu Bian 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第1期56-64,共9页
Great potential of underground gas/energy storage in salt caverns seems to be a promising solution to support renewable energy.In the underground storage method,the operating cycle unfortunately may reach up to daily ... Great potential of underground gas/energy storage in salt caverns seems to be a promising solution to support renewable energy.In the underground storage method,the operating cycle unfortunately may reach up to daily or even hourly,which generates complicated pressures on the salt cavern.Furthermore,the mechanical behavior of rock salt may change and present distinct failure characteristics under different stress states,which affects the performance of salt cavern during the time period of full service.To reproduce a similar loading condition on the cavern surrounding rock mass,the cyclic triaxial loading/unloading tests are performed on the rock salt to explore the mechanical transition behavior and failure characteristics under different confinement.Experimental results show that the rock salt samples pre-sent a diffused shear failure band with significant bulges at certain locations in low confining pressure conditions(e.g.5 MPa,10 MPa and 15 MPa),which is closely related to crystal misorientation and grain boundary sliding.Under the elevated confinement(e.g.20 MPa,30 MPa and 40 MPa),the dilation band dominates the failure mechanism,where the large-size halite crystals are crushed to be smaller size and new pores are developing.The failure transition mechanism revealed in the paper provides additional insight into the mechanical performance of salt caverns influenced by complicated stress states. 展开更多
关键词 Rock salt Cyclic mechanical loading Shear band Dilation band Underground gas storage(UGS)
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Ionization Engineering of Hydrogels Enables Highly Efficient Salt‑Impeded Solar Evaporation and Night‑Time Electricity Harvesting 被引量:2
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作者 Nan He Haonan Wang +3 位作者 Haotian Zhang Bo Jiang Dawei Tang Lin Li 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第1期131-146,共16页
Interfacial solar evaporation holds immense potential for brine desalination with low carbon footprints and high energy utilization.Hydrogels,as a tunable material platform from the molecular level to the macroscopic ... Interfacial solar evaporation holds immense potential for brine desalination with low carbon footprints and high energy utilization.Hydrogels,as a tunable material platform from the molecular level to the macroscopic scale,have been considered the most promising candidate for solar evaporation.However,the simultaneous achievement of high evaporation efficiency and satisfactory tolerance to salt ions in brine remains a challenging scientific bottleneck,restricting the widespread application.Herein,we report ionization engineering,which endows polymer chains of hydrogels with electronegativity for impeding salt ions and activating water molecules,fundamentally overcoming the hydrogel salt-impeded challenge and dramatically expediting water evaporating in brine.The sodium dodecyl benzene sulfonate-modified carbon black is chosen as the solar absorbers.The hydrogel reaches a ground-breaking evaporation rate of 2.9 kg m−2 h−1 in 20 wt%brine with 95.6%efficiency under one sun irradiation,surpassing most of the reported literature.More notably,such a hydrogel-based evaporator enables extracting clean water from oversaturated salt solutions and maintains durability under different high-strength deformation or a 15-day continuous operation.Meantime,on the basis of the cation selectivity induced by the electronegativity,we first propose an all-day system that evaporates during the day and generates salinity-gradient electricity using waste-evaporated brine at night,anticipating pioneer a new opportunity for all-day resource-generating systems in fields of freshwater and electricity. 展开更多
关键词 Solar evaporation Hydrogel evaporators salt impeding Ionization engineering Cyclic vapor-electricity generation
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Characteristic analysis of scattering field in two-layer media by Green's function
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作者 张萍 刘智颖 +2 位作者 阎守国 黄娟 张碧星 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第6期305-315,共11页
The problem of three-dimensional(3D) acoustic scattering in a complex medium has aroused considerable interest of researchers for many years. An ultrasonic scattered field calculating technique is proposed to study th... The problem of three-dimensional(3D) acoustic scattering in a complex medium has aroused considerable interest of researchers for many years. An ultrasonic scattered field calculating technique is proposed to study the scattering echo from strongly scattered materials in a two-layer medium in this work. Firstly, with the high frequency stationary phase method,the Green's function of two-layer fluid media is derived. And then based on the idea of integral equation discretization,the Green's function method is extended to two-layer fluid media to derive the scattering field expression of defects in a complex medium. With this method, the scattering field of 3D defect in a two-layer medium is calculated and the characteristics of received echoes are studied. The results show that this method is able to solve the scattering P wave field of 3D defect with arbitrary shape at any scattering intensity in two-layer media. Considering the circumstance of waterimmersion ultrasonic non-destructive test(NDT), the scattering sound field characteristics of different types of defects are analyzed by simulation, which will help to optimize the detection scheme and corresponding imaging method in practice so as to improve the detection quality. 展开更多
关键词 Green's function method two-layer media scattering field water-immersed detection
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Synergistic effects of carbon cycle metabolism and photosynthesis in Chinese cabbage under salt stress 被引量:1
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作者 Hao Liang Qiling Shi +8 位作者 Xing Li Peipei Gao Daling Feng Xiaomeng Zhang Yin Lu Jingsen Yan Shuxing Shen Jianjun Zhao Wei Ma 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期461-472,共12页
Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and horm... Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress. 展开更多
关键词 Chinese cabbage salt stress Carbon metabolism PHOTOSYNTHESIS CHLOROPLAST
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Cooperative Caching Strategy Based on Two-Layer Caching Model for Remote Sensing Satellite Networks
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作者 Rui Xu Xiaoqiang Di +3 位作者 Hao Luo Hui Qi Xiongwen He Wenping Lei 《Computers, Materials & Continua》 SCIE EI 2023年第5期3903-3922,共20页
In Information Centric Networking(ICN)where content is the object of exchange,in-network caching is a unique functional feature with the ability to handle data storage and distribution in remote sensing satellite netw... In Information Centric Networking(ICN)where content is the object of exchange,in-network caching is a unique functional feature with the ability to handle data storage and distribution in remote sensing satellite networks.Setting up cache space at any node enables users to access data nearby,thus relieving the processing pressure on the servers.However,the existing caching strategies still suffer from the lack of global planning of cache contents and low utilization of cache resources due to the lack of fine-grained division of cache contents.To address the issues mentioned,a cooperative caching strategy(CSTL)for remote sensing satellite networks based on a two-layer caching model is proposed.The two-layer caching model is constructed by setting up separate cache spaces in the satellite network and the ground station.Probabilistic caching of popular contents in the region at the ground station to reduce the access delay of users.A content classification method based on hierarchical division is proposed in the satellite network,and differential probabilistic caching is employed for different levels of content.The cached content is also dynamically adjusted by analyzing the subsequent changes in the popularity of the cached content.In the two-layer caching model,ground stations and satellite networks collaboratively cache to achieve global planning of cache contents,rationalize the utilization of cache resources,and reduce the propagation delay of remote sensing data.Simulation results show that the CSTL strategy not only has a high cache hit ratio compared with other caching strategies but also effectively reduces user request delay and server load,which satisfies the timeliness requirement of remote sensing data transmission. 展开更多
关键词 Information centric networking caching strategy two-layer caching model hierarchical division
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Vibration of a Two-Layer“Metal+PZT”Plate Contacting with Viscous Fluid
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作者 Zeynep Ekicioglu Kuzeci Surkay D.Akbarov 《Computers, Materials & Continua》 SCIE EI 2023年第2期4337-4362,共26页
The present work investigates the mechanically forced vibration of the hydro-elasto-piezoelectric system consisting of a two-layer plate“elastic+PZT”,a compressible viscous fluid,and a rigid wall.It is assumed that ... The present work investigates the mechanically forced vibration of the hydro-elasto-piezoelectric system consisting of a two-layer plate“elastic+PZT”,a compressible viscous fluid,and a rigid wall.It is assumed that the PZT(piezoelectric)layer of the plate is in contact with the fluid and time-harmonic linear forces act on the free surface of the elastic-metallic layer.This study is valuable because it considers for the first time the mechanical vibration of the metal+piezoelectric bilayer plate in contact with a fluid.It is also the first time that the influence of the volumetric concentration of the constituents on the vibration of the hydro-elasto-piezoelectric system is studied.Another value of the present work is the use of the exact equations and relations of elasto-electrodynamics for elastic and piezoelectric materials to describe the motion of the plate layers within the framework of the piecewise homogeneous body model and the use of the linearized Navier-Stokes equations to describe the flow of the compressible viscous fluid.The plane-strain state in the plate and the plane flow in the fluid take place.For the solution of the corresponding boundary-value problem,the Fourier transform is used with respect to the spatial coordinate on the axis along the laying direction of the plate.The analytical expressions of the Fourier transform of all the sought values of each component of the system are determined.The origins of the searched values are determined numerically,after which numerical results on the stress on the fluid and plate interface planes are presented and discussed.These results are obtained for the case where PZT-2 is chosen as the piezoelectric material,steel and aluminum as the elastic metal materials,and Glycerin as the fluid.Analysis of these results allows conclusions to be drawn about the character of the problem parameters on the frequency response of the interfacial stress.In particular,it was found that after a certain value of the vibration frequency,the presence of the metal layer in the two-layer plate led to an increase in the absolute values of the above interfacial stress. 展开更多
关键词 Metal+piezoelectric two-layer plate compressible viscous fluid mechanical forced vibration interfacial stress resonance frequency
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The source of lithium in Lakkor Co Salt Lake on Qinghai-Tibet Plateau,China:evidence from hydrochemical characteristics and boron isotope
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作者 Zheng Yan Li Bin Kai Li +2 位作者 Mao-Yong He Xue Qin Wen Jiang Di Zhou 《Acta Geochimica》 EI CAS CSCD 2024年第5期933-946,共14页
The availability of lithium resources is of great significance for the development of modern technologies,as well as for civil and military industries.The Qinghai-Tibet Plateau is a region known for its abundance of l... The availability of lithium resources is of great significance for the development of modern technologies,as well as for civil and military industries.The Qinghai-Tibet Plateau is a region known for its abundance of lithium-rich salt lakes.However,the specific origin of lithium in these lakes is still unknown,which hinders the advancement of the lithium resource business in this region.To research this issue,this study involved the collection of 20 samples from Lakkor Co Salt Lake on Qinghai-Tibet Plateau,encompassing samples of surface brine,cold springs,fresh lakes,and recharge rivers.The composition of anions and cations in these samples was determined.Furthermore,the analysis extensivelyutilizedthePiperthree-linediagram,Gibbs model,and ion proportion coefficient.The findings of this study indicate that as the moves from the recharge water system to salt lake,there is a transition in water type from strong carbonate to moderate carbonate and weak carbonate,as well as Na sulfate.This research based on a similar source of both lithium and boron,utilized ion correlation analysis and boron isotope study in the Lakkor Co area,and analyzed the source and transporting process of lithium.The main origin of lithium in Lakkor Co is the dissolution of lithiumrich rocks,recharge water systems,and deep hydrothermal fluids.These findings are highly significant in enhancing the foundational data of lithium-rich brine resources in the Qinghai-Tibet Plateau and are beneficial for assessing the future development of such deposits. 展开更多
关键词 LITHIUM Hydrochemical Li-rich salt lake Boron isotope
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Growth kinetics of titanium carbide coating by molten salt synthesis process on graphite sheet surface
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作者 Xiaoyu Shi Chongxiao Guo +4 位作者 Jiamiao Ni Songsong Yao Liqiang Wang Yue Liu Tongxiang Fan 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第8期1858-1864,共7页
The synthesis of carbide coatings on graphite substrates using molten salt synthesis(MSS),has garnered significant interest due to its cost-effective nature.This study investigates the reaction process and growth kine... The synthesis of carbide coatings on graphite substrates using molten salt synthesis(MSS),has garnered significant interest due to its cost-effective nature.This study investigates the reaction process and growth kinetics involved in MSS,shedding light on key aspects of the process.The involvement of Ti powder through liquid-phase mass transfer is revealed,where the diffusion distance and quantity of Ti powder play a crucial role in determining the reaction rate by influencing the C content gradient on both sides of the carbide.Furthermore,the growth kinetics of the carbide coating are predominantly governed by the diffusion behavior of C within the carbide layer,rather than the chemical reaction rate.To analyze the kinetics,the thickness of the carbide layer is measured with respect to heat treatment time and temperature,unveiling a parabolic relationship within the temperature range of 700-1300℃.The estimated activation energy for the reaction is determined to be 179283 J·mol^(-1).These findings offer valuable insights into the synthesis of carbide coatings via MSS,facilitating their optimization and enhancing our understanding of their growth mechanisms and properties for various applications. 展开更多
关键词 titanium carbide GRAPHITE molten salt kinetic analysis
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The transcription factor ZmNAC84 increases maize salt tolerance by regulating ZmCAT1 expression
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作者 Yitian Pan Tong Han +2 位作者 Yang Xiang Caifen Wang Aying Zhang 《The Crop Journal》 SCIE CSCD 2024年第5期1344-1356,共13页
Salt stress severely affects plant growth and yield.The transcription factor NAC plays a variety of important roles in plant abiotic stress,but we know relatively little about the specific molecular mechanisms of NAC ... Salt stress severely affects plant growth and yield.The transcription factor NAC plays a variety of important roles in plant abiotic stress,but we know relatively little about the specific molecular mechanisms of NAC in antioxidant defense.Here,our genetic studies reveal the positive regulation of salt tolerance in maize by the transcription factor ZmNAC84.Under salt stress,overexpression of ZmNAC84 in maize increased the expression of ZmCAT1,enhanced CAT activity,and consequently reduced H_(2)O_(2) accumulation,thereby improving salt stress tolerance in maize.Whereas RNA interference-mediated knockdown of ZmNAC84 produced the opposite effect.Subsequently,we found that ZmNAC84 directly binds to and regulates the expression of the ZmCAT1 promoter,and the hybridized material also demonstrated that ZmCAT1 is a downstream target gene of ZmNAC84.In addition,phenotypic and biochemical analyses indicated that ZmCAT1 positively regulated salt tolerance by regulating H_(2)O_(2) accumulation under salt stress.Taken together,these results reveal the function of ZmNAC84 in regulating ZmCAT1-mediated antioxidant defense in response to salt stress in plants. 展开更多
关键词 MAIZE ZmNAC84 ZmCAT1 salt stress
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Wild soybean(Glycine soja)transcription factor GsWRKY40 plays positive roles in plant salt tolerance
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作者 Minglong Li Man Xue +7 位作者 Huiying Ma Peng Feng Tong Chen Xiaohuan Sun Qiang Li Xiaodong Ding Shuzhen Zhang Jialei Xiao 《The Crop Journal》 SCIE CSCD 2024年第3期766-775,共10页
Wild soybean(Glycine soja),a relative of cultivated soybean,shows high adaptability to adverse environmental conditions.We identified and characterized a wild soybean transcription factor gene,GsWRKY40,that promotes p... Wild soybean(Glycine soja),a relative of cultivated soybean,shows high adaptability to adverse environmental conditions.We identified and characterized a wild soybean transcription factor gene,GsWRKY40,that promotes plant salt stress.GsWRKY40 was highly expressed in wild soybean roots and was up-regulated by salt treatment.GsWRKY40 was localized in nucleus and demonstrated DNA-binding activities but without transcriptional activation.Mutation and overexpression of GsWRKY40 altered salt tolerance of Arabidopsis plants.To understand the molecular mechanism of GsWRKY40 in regulating plant salt resistance,we screened a cDNA library and identified a GsWRKY40 interacting protein GsbHLH92 by using yeast two-hybrid approach.The physical interaction of GsWRKY40 and GsbHLH92 was confirmed by co-immunoprecipitation(co-IP),GST pull-down,and bimolecular fluorescence complementation(BiFC)techniques.Intriguingly,co-overexpression of GsWRKY40 and GsbHLH92 resulted in higher salt tolerance and lower ROS levels than overexpression of GsWRKY40 or GsbHLH92 in composite soybean plants,suggesting that GsWRKY40 and GsbHLH92 may synergistically regulate plant salt resistance through inhibiting ROS production.qRT-PCR data indicated that the expression level of GmSPOD1 gene encoding peroxidase was cooperatively regulated by GsWRKY40 and GsbHLH92,which was confirmed by using a dual luciferase report system and yeast one-hybrid experiment.Our study reveals a pathway that GsWRKY40 and GsbHLH92 collaboratively up-regulate plant salt resistance through impeding GmSPOD1 expression and reducing ROS levels,providing a novel perspective on the regulatory mechanisms underlying plant tolerance to abiotic stresses. 展开更多
关键词 Wild soybean Transcription factor salt stress ROS
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Different Approaches to Reduce Salinity in Salt-Affected Soils and Enhancing Salt Stress Tolerance in Plants
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作者 Amra Bratovcic 《Agricultural Sciences》 2024年第8期830-847,共18页
Salt stress is one of the most harmful environmental stresses in recent times and represents a significant threat to food security. Soil salinization is caused by spontaneous natural processes of mineral dissolution a... Salt stress is one of the most harmful environmental stresses in recent times and represents a significant threat to food security. Soil salinization is caused by spontaneous natural processes of mineral dissolution and human activities such as inappropriate irrigation practices. Natural geological progressions like weathering of rocks, arid climate, and higher evaporation, as well as anthropogenic activities, including the use of brackish water for irrigation, and poor tillage operations, are the foremost causes of soil salinization. Typical characteristics of saline soils are salt stress, high pH, and lack of organic carbon, as well as low availability of nutrients. Disruption of precipitation patterns as well as high average annual temperatures due to climate change additionally negatively affects the process of soil salinization. Productivity and ability to support crop growth are reduced on saline soil. Salinity-induced stress reduces plant growth by modulating the antioxidative system and nutrient orchestration. The aim of this work is to show that the mentioned problems can be alleviated in several ways such as the addition of biochar, exogenous application of several elicitors, seed priming, etc. Research has shown that the addition of biochar can significantly improve the recovery of saline soil. The addition of biochar has no significant effect on soil pH, while the cation exchange capacity of the soil increased by 17%, and the electrical conductivity of the saturated paste extract decreased by 13.2% (depends on the initial salinity and the type of biochar raw material). Moreover, biochar enriched with silicon increases the resistance of bananas to salt stress. In addition, exogenous application of several elicitors helps plants to alleviate stress by inducing stress-related physicochemical and molecular changes (selenium, sulfur, silicon, salicylic acid). Finally, seed priming showed positive effects on metabolomics, proteomics and growth of plants subjected to abiotic stress. Priming usually involves immersing the seed in a solution for a period of time to induce physiological and metabolic progression prior to germination. 展开更多
关键词 salt-Affected Soils salt Stress BIOCHAR ELICITORS Seed Priming
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AcWRKY28 mediated activation of AcCPK genes confers salt tolerance in pineapple(Ananas comosus)
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作者 Qiao Zhou Samaranayaka Vidana Gamage Nirosha Priyadarshani +11 位作者 Rongjuan Qin Han Cheng Tiantian Luo Myat Hnin Wai Mohammad Aqa Mohammadi Yanhui Liu Chang Liu Hanyang Cai Xiaomei Wang Yeqiang Liu Yuan Qin Lulu Wang 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第2期398-412,共15页
Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRKY transcription factor (TF) family, modulate t... Unfavorable environmental cues severely affect crop productivity resulting in significant economic losses to farmers. In plants, multiple regulatory genes, such as the WRKY transcription factor (TF) family, modulate the expression of defense genes. However, the role of the pineapple WRKY genes is poorly understood. Here, we studied the pineapple WRKY gene, AcWRKY28, by generating AcWRKY28 over-expressing transgenic pineapple plants. Overexpression of AcWRKY28 enhanced the salt stress resistance in transgenic pineapple lines. Comparative transcriptome analysis of transgenic and wild-type pineapple plants showed that “plant-pathogen interaction” pathway genes, including 9calcium-dependent protein kinases (CPKs), were up-regulated in AcWRKY28 over-expressing plants. Furthermore, chromatin immunoprecipitation and yeast one-hybrid assays revealed AcCPK12, AcCPK3, AcCPK8, AcCPK1, and AcCPK15 as direct targets of AcWRKY28. Consistently, the study of AcCPK12 over-expressing Arabidopsis lines showed that AcCPK12 enhances salt, drought, and disease resistance. This study shows that AcWRKY28 plays a crucial role in promoting salt stress resistance by activating the expression of AcCPK genes. 展开更多
关键词 PINEAPPLE AcWRKY AcCPK Transcription factor salt stress CHIP
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Biomineralization of soil with crude soybean urease using different calcium salts
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作者 Yajie Weng Junjie Zheng +2 位作者 Hanjiang Lai Mingjuan Cui Xingzhi Ding 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第5期1788-1798,共11页
Calcium salt is an important contributing factor for calcium-based biomineralization.To study the effect of calcium salt on soil biomineralization using crude soybean urease,the calcium salts,including the calcium chl... Calcium salt is an important contributing factor for calcium-based biomineralization.To study the effect of calcium salt on soil biomineralization using crude soybean urease,the calcium salts,including the calcium chloride (CaCl_(2)),calcium acetate ((CH_(3)COO)_(2)Ca) and calcium nitrate (Ca(NO_(3))_(2)),were used to prepare the biotreatment solution to carry out the biomineralization tests in this paper.Two series of biomineralization tests in solution and sand column,respectively,were conducted.Scanning electron microscopy (SEM) and X-ray diffraction (XRD) were performed to determine the microscopic characteristics of the precipitated calcium carbonate (CaCO_(3)) crystals.The experimental results indicate that the biomineralization effect is the best for the CaCl2 case,followed by (CH_(3)COO)_(2)Ca,and worst for Ca(NO_(3))_(2) under the test conditions of this study (i.e.1 mol/L of calcium salt-urea).The mechanism for the effect of the calcium salt on the biomineralization of crude soybean urease mainly involves: (1) inhibition of urease activity,and (2) influence on the crystal size and morphology of CaCO_(3).Besides Ca^(2+) ,the anions in solution can inhibit the activity of crude soybean urease,and NO_(3)− has a stronger inhibitory effect on the urease activity compared with both CH_(3)COO^(−) and Cl^(−) .The co-inhibition of Ca^(2+) and NO_(3)− on the activity of urease is the key reason for the worst biomineralization of the Ca(NO_(3))_(2) case in this study.The difference in biomineralization between the CaCl_(2) and (CH_(3)COO)_(2) Ca cases is strongly correlated with the crystal morphology of the precipitated CaCO_(3). 展开更多
关键词 BIOMINERALIZATION Crude soybean urease Calcium salt Influence mechanism
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Soaking Rice Seeds in High Concentration Salt Solution Improved Salt Tolerance at the Seedling Stage
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作者 Jing LIN Weiwei CAO +1 位作者 Fan WANG Xianwen FANG 《Agricultural Biotechnology》 2024年第5期16-18,共3页
[Objectives]This study was conducted to enhance the salt tolerance of current rice varieties at the seedling stage and fulfill the urgent requirement for salt-tolerant rice varieties in coastal tidal flats.[Methods]Fo... [Objectives]This study was conducted to enhance the salt tolerance of current rice varieties at the seedling stage and fulfill the urgent requirement for salt-tolerant rice varieties in coastal tidal flats.[Methods]Four high-generation stable rice lines with diverse salt tolerance were employed as test materials,and four NaCl concentration gradients were established for seed soaking treatment.[Results]The seedling survival rate of line 151465 underwent significant alterations after soaking with four different salt concentrations,and the survival rate was the highest after treatment with 1.8%NaCl for 1 d,reaching 65.2%.The average survival rate of other three lines with different salt tolerance reached 62%after soaking with 1.8%NaCl for 1 d,which was significantly higher than those of the 2.2%NaCl and 0%NaCl treatments.[Conclusions]This study provides a basis for reducing the effect of abiotic stress on rice growth and development and improving the utilization rate of saline-alkali land. 展开更多
关键词 RICE salt water Seed soaking salt tolerance Seedling stag
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