Solutions to Insecurity Problems in Seed Production of Two-line Hybrid Rice

The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situation were pointed out in this paper.The three main reasons for unsafety in seed production of twoline hybrid rice were unsuitable site selection,high critical sterility-inducing temperature and the drift of critical temperature.In this paper,strategies and measures were put forward based on many years of practice.It could minimize the risk in seed production of two-line hybrid rice by selecting dual-purpose genic male sterile line with lower critical sterility-inducing temperature and long lower temperature resistant time.Based on the climate data and climatic demands of the ＂three safe-periods＂ in seed production,a new idea for determining appropriate bases and periods for seed production was proposed by using computer technology,which solved the aimless selection of sites and periods for the seed production of two-line hybrid rice.Besides,we established a system of single plant selection and identification method and original seed propagation with cyclic cold water,which could reduce the generation number of original seed propagation in seed production and avoid the drift of critical sterility-inducing temperature.This paper improved the seed production safety in the three aspects of seed nature,seed source and seed production site.

Study on Mutagenic Effect of Space Flight in Two-line Sterile Rice

Seeds of two-line sterile rice cultivars Zhu 1S and Lu 18S were carried into space by ＂Shijian 8＂ breeding recoverable satellite, then planted in ground. Mutagenic effects from space induction were compared with those from y-irradiation and complex processing of space induction and y-irradiation. The results showed that agronomic effect was stimulated in Mo progenies of the two-line sterile rice varieties treated by space flight, and their radiosensitivities to the irradiation of space flight performed non-sensitive. The order of mutation frequency was determined to be SP ＋ γ 〉 γ 〉 SP in M2 generation. And a series of mutated elites（individuals） were screened. Physiological indices of mutants screened like the activity of protective enzymes were measured to explore the physiological and biochemical basis of biological effect in space environment to two-line sterile rice. The results of this study show that space mutation breeding is an effective novel mean for breeding.

Effects of Nitrogen Quantity on Grain-filling Characters of Two-line Hybrid Rice with Large Ear

[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made use of to study on effects of N fertilizer in different quantities （LN： 90 kg/hm2;MN： 180 kg/hm2;HN： 270 kg/hm2） on plumpness and grain-filling characters. [Result] When N fertilizers were excessive, for inferior grains, grain-filling rate decreased and grain-filling time extended, resulting in plumpness decline after degradation of leaves＇ function. When N fertilizers were inadequate, maximal and average grain-filling rates decreased and the differences between superior and inferior grains in grain-filling rate increased, leading to decline of grain＇s weight and plumpness degree. On the other hand, quantity of N fertilizers had little effect on superior grains in plumpness. [Conclusion] The research provided references for reasonable use of N fertilizer and improvement of rice yield and N use.

Breeding of National Authorized Two-line Hybrid Rice Yanliangyou 2208

The breeding processes, characteristics and comprehensive characters of two-line hybrid rice Yanliangyou 2208 and its parents （Yan 220S and Yanhui 888） taking part in the national rice regional test of southern rice regions were introduced. Yanliangyou 2208 was approved by the National Crop Variety Appraisal Committee in 2013. The achievement has been transformed successfully and has been applied in production. It has good development and promotion prospects.

Construction of Accumulated Temperature Effect Model for Fertility Alteration of Two-line Sterile Line P88S

[Objective]The research aimed at constructing an accumulated temperature effect model for fertility alteration of two-line hybrid rice P88S.[Method]Analyze the experiment datas of fertility alteration of P88S in Hainan from 2005 to 2006.[Result]The fertilities of P88S with temperatures ranging from 26 to 28 ℃ were determined by the absolute values and the accumulation effects of the temperature.An accumulation effect model of temperatures based on the bistable state was obtained.[Conclusion]The temperature asymmetry of fertility alteration for two-line sterility line in spring and autumn can be elucidated effectively using the accumulated temperature effect model,namely,alternating from fertile to sterile requires higher temperatures,and alternating from sterile to fertile requires lower temperatures.

Thoughts and Practice on Some Problems about Research and Application of Two-Line Hybrid Rice

The main problems about research and application of two-line hybrid rice were reviewed, including the confusing nomenclature and male sterile lines classification, the unclear characteristics of photoperiod and temperature responses and the unsuitable site selection for male sterile line and hybrid dce seed production. In order to efficiently and accurately use dual-purpose genic male sterile lines, four types, including PTGMS （photo-thermo-sensitive genic male sterile rice）, TGMS （thermo-sensitive genic male sterile rice）, reverse PTGMS and reverse TGMS, were proposed. A new idea for explaining the mechanism of sterility in dual-purpose hybrid rice was proposed. The transition from sterile to fertile was involved in the cooperative regulation of major-effect sterile genes and photoperiod and/or temperature sensitive ones. The minor-effect genes with accumulative effect on sterility were important factors that affected the critical temperature of sterility transfer. In order to make better use of dual-purpose lines, the characterization of responses to photoperiod and temperature of PTGMS should be made and the identification method for the characterization of photoperiod and temperature responses of PTGMS should also be put forward. The optimal ecological site for seed production could be determined according to the historical climate data and the requirements for the meteorological conditions during the different periods of seed production.

Research on Theories and Techniques of Irrigation for Safeguarding Seed Production of Two-Line Hybrid Rice

By inducing frequency, intensity and duration of lower temperature in the middle and last ten-day periods in August in the rice-growing areas of southern China, increasing temperature for safe seed production was defined as 2℃. During the sensitive period of fertility, characters of panicle height and canopy structure of TGMS rice, Pei＇ai64S, were measured. Results showed that temperature changes caused by irrigation in fields were below 40 cm of rice plant, and heating effect was significant at 20 cm when the temperature was increased by 3.1 ℃. For the present study, the following irrigation techniques were put forth： the water depth of 15-20 cm, current water used, irrigating after 17：00 and bailing at 10：00 in sunny or cloudy weather, irrigating on whole day, in shady or rainy weather, increasing inflows and outflows in large fields. In the present experiment, pollen fertility and self-fertilized seed setting rate accepted that the techniques were feasible and effective for against lower temperature and safeguarding seed production of two-line hybrid rice.

Causes of Poor Grain Plumpness of Two-Line Hybrids and Their Relationships to the Contents of Hormones in the Rice Grain

This study was designed to elucidate the grain filling characteristics and the causes of poor grain plumpness of some two-line hybrid rice combinations and their hormonal mechanism. Six varieties, including three two-line hybrid rice combinations, that show differences in seed-setting and grain filling, were used. And the contents of starch, sucrose, zeatin （Z） ＋ zeatin riboside （ZR）, indole-3-acetic acid （IAA）, and abscisic acid （ABA）, the ethylene evolution rate, activities of sucrose synthase （SuSase） and starch synthase （StSase） in grains, the seed-setting and grain filling rate were investigated. The correlations amongst these were analyzed. The results showed that the poor grain filling of two-line hybrids was mainly attributed to the higher unfilled grain rate and the lower filling degree of inferior grains. During the early and mid grain filling periods, the sucrose content in inferior grains was greater than that in superior grains for the combinations with poor grain filling, indicating that the substrate concentration was not the principal factor for their slow grain filling and poor grain plumpness of the inferior grains of two-line hybrids. Z ＋ ZR, IAA, and ABA in superior grains were obviously greater than those in inferior grains at early grain filling stage. The maximum and mean contents of Z ＋ ZR, IAA, and ABA were positively very significantly correlated with the maximum and mean grain-filling rate, filling degree, and grain weight. The evolution rate of ethylene was greater in inferior grains than in superior grains and greater for the combinations with poor grain plumpness than those with good grain plumpness at the early or mid filling stages. The evolution rate of ethylene was negatively and significantly correlated with the grain filling rate, the grain filling degree, and the grain weight. Spraying ethephon （ethylene-releasing agent） at the early grain filling stage increased the evolution rate of ethylene, reduced the ABA content and activities of SuSase and StSase, and decreased the grain filling degree and the grain weight. The results were reversed when cobatous nitrate （an inhibitor of ethylene synthesis） was applied. The results suggested that the hormones and their balance play a role in the regulation of grain filling and enzymatic activities, and the poor grain filling is attributed to the low contents of Z ＋ ZR, IAA, and ABA, and the high evolution rate of ethylene in the inferior grains of some two-line hybrid rice combinations. The results suggested that hormones play important roles in the grain filling of some two-line hybrid rice combinations, and their filling degree can be improved by regulating the hormonal contents.

Inheritance Pattern of Two-line Hybrid Rice Resistance to False Smut

[Objective] The aim of this study was to explore the inheritance pattern of the resistance of two-line hybrid rice to false smut. [Method] A moderate susceptible TGMS line 33S as the female parent, was crossed with 30 susceptible restorer lines and 23 resistant restorer lines. Then false smut resistance of the hybrid F1 and parents were identified. [Result] All F1 of 33S crossed with 30 susceptible re- storer lines were susceptible to rice false smut. The F1 of 33S crossed with 23 re- sistant restorer lines showed dominant, incompletely dominant or recessive inheri- tance. Dominant and incompletely dominant inheritance accounted for 87.0%. The diseased panicle rate, disease index, average diseased grains per panicle, and the highest diseased grains per panicle of F1 shared highly significant positive correla- tions with corresponding indicators of restorer lines. [Conclusion] This study provides references for genetic improvement of two-line hybrid rice.

Analysis on Genetic Differences between Parents of Two-line Medium Indica Hybrid Rice by SSR Markers

The genetic diversity of 50 parents or improved lines of two-line medium Indica hybrid rice were analyzed based on 48 pairs of SSR markers. The results showed that the clustering results were highly accordant with the parental pedigree information of the tested materials. The male sterile lines and the restoring lines were clustered into 2 distinct groups （Ⅰ and Ⅱ）, respectively. There were no significant difference in intra-group average genetic distances （GD） between the 2 groups. Most of the male sterile lines from Hunan Longping High-tech Seed Science Research Institute Co., Ltd. were grouped into a single subgroup （Ⅰ-4） under the male sterile group with the largest average GD compared with the rest male sterile subgroups. Huazhan derived lines and Guangzhou inbred lines were founded to be independent of 9311 and its derivatives or closely-related lines. Meanwhile, they have obviously been differentiated into 2 subgroups （Ⅱ-2 and Ⅱ-3）. The lines derived from Huazhan （or genetic closely related lines） showed a genetic homogenization phenomenon. Therefore, the Huazhan-derived lines should be further improved by the utilization of germplasms with good quality, high resistance and wide adaptability from Southern China to widen the GD between Huazhan derived lines and the male sterile subgroup developed by Hunan Longping High-tech Seed Science Research Institute Co., Ltd., so as to further promote the heterosis level between the groups.

Semi-Markovian Model of Two-Line Queuing System with Losses

In the present paper, to build model of two-line queuing system with losses GI/G/2/0, the approach introduced by V.S. Korolyuk and A.F. Turbin, is used. It is based on application of the theory of semi-Markov processes with arbitrary phase space of states. This approach allows us to omit some restrictions. The stationary characteristics of the system have been defined, assuming that the incoming flow of requests and their service times have distributions of general form. The particular cases of the system were considered. The used approach can be useful for modeling systems of various purposes.

Breeding of a Water-saving Drought-resistant Two-line Hybrid Rice Variety Wanliangyou 1008

Drought is one of the important factors limiting rice production,and the application of new water-saving rice varieties is urgently needed in rice production.Wanliangyou 1008 bred by Institute of Rice Research,Anhui Academy of Agricultural Sciences is a new two line hybrid rice variety with some good traits,including high drought resistance,high yield and good disease resistance.Wanlingyou 1008 was obtained by crossing drought-resistant high-quality restorer line DJ1008 and disease-resistant two-line sterile line Wan 25S.In this paper,the breeding process,characteristics and seed production technology of the variety were described in detail.

Performance and High-Yield Cultural Techniques of Two-Line Hybrid Rice Xinlingyou998 at Direct Seeding Cultivation

Xinliangyou 998 is a new indica two-line hybrid rice variety using Xin＇er Sx H0998 as parent. The variety was introduced and demonstrated in Yingshan County in 2016, and showed intermediate growth duration, strong tillering ability, firm culm, tolerance to high fertility and resistance to lodging, tolerance to high tempera- ture and wide range of adaptation, etc. The high-yield cultural techniques were con- cluded.

Breeding and Application Techniques of a New Rice Variety Liliang-you 3822 with High Yield and Disease Resistance

Liliangyou 3822 is a novel indica hybrid rice variety that exhibits disease resistance,high yield,lodging resistance,and late maturity.It employs a self-selected two-line sterile line,Li 38S,and a self-selected restorer line,R22.This variety was subjected to a regional test of indica late-maturing groups in the middle and lower reaches of the Yangtze River in 2020.The results demonstrated that the average yield of the variety was 9.95 t/hm 2,which was 10.67%higher than that of the control Fengliangyou 4,indicating a highly significant yield increase.In the continuous test in 2021,the average yield was 9.74 t/hm 2,representing a 6.52%increase over the control,which also exhibited a significant increase.Finally,the average yield of the two years regional test was 9.84 t/hm 2,which was 8.58%higher than that of the control.In the 2021 production test,the average yield of the variety was 9.32 t/hm 2,which was 12.19%higher than that of the control,indicating a remarkably significant yield increase.In 2022,the variety was validated by the National Crop Variety Approval Committee(GSD 20220143).

四川盆地东部中二叠统茅口组孤峰段展布特征及其油气地质意义

基于钻井岩-电特征和野外露头特征,结合区域沉积-构造格局,利用宏观及微观观察、主微量及稀土元素测试、总有机碳含量和镜质体反射率测定等手段对四川盆地东部中二叠统茅口组孤峰段展布特征、沉积环境和成藏条件进行研究。结果表明:①川东地区茅口组孤峰段的存在,证实茅口中、晚期受构造-沉积分异的影响,出现槽台格局。在茅口组二段B亚段沉积期(茅二B沉积期),川东地区孤峰段主要分布在盆地东北角。在茅口组二段A亚段沉积期(茅二A沉积期),孤峰段向南扩大到渠县—大竹—梁平,呈北西-南东向带状展布,厚度在0.8~26.6 m,川西北-川北则对应茅口组三段(茅三段)或茅口组四段(茅四段)。②孤峰段硅质页岩富含硅质放射虫,有机质含量高,具有典型的深水海槽相沉积特征。地球化学测试数据反映硅质页岩为深水缺氧还原环境且盐度较高,硅质来源以生物硅为主。③孤峰段烃源岩为一套富有机质的优质烃源岩,总有机碳含量整体较高、变化范围大,分布在1.69%~38.27%,平均值为11.51%,处于成熟-过成熟阶段。④川东地区孤峰段优质烃源岩可通过断裂与开江-梁平海槽礁滩相储层沟通,构成“下生上储”的源-储关系;此外,也可与茅口组优势滩相储层形成“旁生侧储”的成藏组合。川东地区孤峰段有望作为区域内优质烃源岩的接力勘探层系,对研究区内下一步油气勘探开发具有重要意义。

新型110 kV扩大内桥接线的电压切换回路

扩大内桥接线方式已经在110 kV变电站得到广泛应用,并制定了典型设计方案,但相应的二次电压切换回路还未建立统一标准。对当前电压切换回路存在的缺陷进行详细分析,针对总结出的问题提出一种改进的电压切换回路方案,并进行效果检查,结果表明该方案能使二次电压切换情况与一次运行状况相符,并且可避免母线二次电压误并列的情况,为电力系统安全稳定运行提供了保障。

TLE orbit determination using simplex method

Two-Line Element(TLE)datasets are the only orbital data source of Earth-orbiting space objects for many civil users for their research and applications.The datasets have uneven qualities that may affect the reliability of the propagated positions of space objects using a single TLE.The least squares approach to use multiple TLEs also suffers from the poor quality of some TLEs,and reliable error information cannot be available.This paper proposes a simplex algorithm to estimate an optimal TLE from multiple TLEs and obtain the uncertainty of each element.It is a derivative-free technique that can deal with various orbit types.Experiments have demonstrated that using the TLE estimated from the simplex method is more reliable,stable,and effective than those from the batch least squares method.As an application example,the optimal TLE and its uncertainty are used for predicting the fallen area,keeping the actual fallen site in the prediction areas.

断层封闭性研究进展

对断层封闭性的研究现状进行了总结，概述了断层封闭性的理论机理、影响因素和评价方法；对其中存在的一些问题如封闭机理及与其有关的评价方法、构造反转与断层封闭的关系以及断层封闭性的三维研究问题进行了讨论。

重磁遗传算法三维反演中高速计算及有效存储方法技术

将地下场源区域规则划分成很多小长方体单元 ,并且通过反演确定这些单元的物性变化 ,勾画出场源的分布图像 ,这种方式逐步成为重磁反演 ,特别是三维反演的重要方向 ;遗传算法等非线性技术进行该类反演将逐步成为发展趋势 .本文指出 ,在应用遗传算法进行该类反演过程中 ,隐含着数据量较大时超常规的计算量 ,它已成为制约该类反演充分发挥作用的瓶颈问题 ;同时 ,本文提出了针对性的分离并存储几何格架的计算策略、以及独特的几何格架等效压缩存储技术 ,可以从根本上提高非线性反演计算速度 ,为该类反演的有效应用奠定了坚实的基础 .

大港油田大张坨断层三维封闭特征

断层封闭大致有两种类型 ,一是岩性封闭型 ,断层上、下盘砂泥岩并置造成封闭 ;二是断面型 ,即泥岩涂抹使断面压差很高而封闭。对于由并置作用引起的断层封闭性 ,可在Allan模式的基础上定量评价 ;对于因泥岩涂抹造成的断层封闭 ,可应用断面物质涂抹法 ,用泥岩涂抹系数、泥岩涂抹比率、泥岩涂抹潜力等属性参数描述其空间封闭性。在大港油田千米桥地区大张坨断层的三维封闭性定量评价中 ,用实测地层压力来标定其泥岩涂抹属性参数 ,确定大张坨断层的中、下部都是封闭的。