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Characterisation and separation of infectious bursal disease virus-like particles using aqueous two-phase systems
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作者 Hui Yi Leong Xiao-Qian Fu +2 位作者 Xiang-Yu Liu Shan-Jing Yao Dong-Qiang Lin 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2023年第5期72-78,共7页
Infectious bursal disease(IBD)causes considerable economic losses in the commercial poultry industry worldwide.The principal way to control IBD virus(IBDV),the causative agent of IBD,is still through vaccination progr... Infectious bursal disease(IBD)causes considerable economic losses in the commercial poultry industry worldwide.The principal way to control IBD virus(IBDV),the causative agent of IBD,is still through vaccination programs.Virus-like particles(VLPs)are recognised as a safe and potent recombinant vaccine platform.This research work explores the characterisation and separation of infectious bursal disease virus-like particles(IBD-VLPs)from crude feedstock.Various characteristics were studied with highperformance size-exclusion chromatography(HP-SEC),sodium dodecyl sulphate–polyacrylamide gel electrophoresis(SDS-PAGE)and transmission electron microscopy(TEM)analyses.Subsequently,the separation of IBD-VLPs using polyethylene glycol(PEG)/sodium citrate-based aqueous two-phase systems(ATPSs)was conducted and optimised.Moreover,a scale-up study of the best ATPS constituted of 15%PEG 6000,11%sodium citrate and 10%crude feedstock was performed to compare the separation performance of IBD-VLPs with and without centrifugation-assisted.The results indicated that the optimised ATPS with centrifugation-assisted for both 5 g and 50 g systems showed good recovery of IBDVLPs of>97%in the interphase between the PEG-rich top and salt-rich bottom phases.These optimised systems also showed high removal efficiencies of impurities of>95%.The results demonstrated that aqueous two-phase extraction could be a promising technology for efficient VLPs separation. 展开更多
关键词 Aqueous two-phase extraction Infectious bursal disease virus POLYMERS SALT SEPARATION Virus-like particle
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Extraction of Theanine from Waste Liquid of Tea Polyphenol Production in Aqueous Two-phase Systems with Cationic and Anionic Surfactants 被引量:8
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作者 张军伟 王艳 彭奇均 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2013年第1期31-36,共6页
Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactanttwo-phase system (ASTP) with cationic surfactant (CTAB) and anionic surfactant (SDS). Results indicate that ... Extraction of theanine from waste liquid of tea polyphenol production was studied in aqueous surfactanttwo-phase system (ASTP) with cationic surfactant (CTAB) and anionic surfactant (SDS). Results indicate that the region of ASTP is narrow and there is only a two-phase region of cationic surfactant. The increase in concentrations of NaBr and Na2SO4 are beneficial to the formation of ASTP. Theanine concentration in the bottom phase increases with increasing concentration of theanine, whereas the Partition coefficient and extraction rate only change a littlewhen the concentration of theanine is above 0.2 g.L-'. With the increase of SDS concentration, the phase ratio and the partition coefficient decrease, while the extraction efficiency of theanine increases and the concentration of theanine changes a little in the range from 2.4/7.5 to 2.8/7.2 for SDS/CTAB ratio. The temperature has a notable ef- fect on the concentration of theanine in the bottom phase, partition coefficient and extraction rate of theanine. The increase of waste liquid decreases the phase ratio, increases the concentration and extraction rate of theanine in the bottom ohase, since the orotein and the saccharide enter the bottom nhase with theanine. 展开更多
关键词 aqueous two-phase system SURFACTANT THEANINE waste liquid of tea polyphenol production EXTRACTION
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Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system 被引量:6
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作者 徐莹 何国庆 李景军 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE EI CAS CSCD 2005年第11期1087-1094,共8页
This paper presents the evaluation of an aqueous two-phase system (ATPS) for extracting elastase produced by Bacillus sp. EL31410. The elastase and cell partition behavior in polyethylene glycol (PEG)/salt systems... This paper presents the evaluation of an aqueous two-phase system (ATPS) for extracting elastase produced by Bacillus sp. EL31410. The elastase and cell partition behavior in polyethylene glycol (PEG)/salt systems was investigated. The suitable system for elastase extraction was PEG/KHEPO4-KEHPO4, in which elastase is mainly partitioned into the PEG-rich phase, while the cells remained in the other phase. The influence of defined system parameters (e.g. PEG molecular mass, pH, NaCl addition) on the partitioning behavior of elastase is described. The concentration of phase forming components, PEG and KHEPO4-KEHPO4, was optimized for elastase recovery by means of response surface methodology, and it was found that they greatly influenced extraction recovery. The optimal ATPS was 23.1% (w/w) PEG 2000 and 11.7% (w/w) KHEPO4-KEHPO4. The predicted recovery was about 89.5%, so this process is suggested to be a rapid and convenient method for elastase extraction. 展开更多
关键词 ELASTASE Aqueous two-phase system (ATPS) BIOSEPARATION PURIFICATION Optimization Response surface methodology (RSM)
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Measurement and Correlation of Equilibrium Data for Aqueous Two-phase System Ethanol+Water+ K_2HPO_4 被引量:4
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作者 LINJin-qing TANPing-hua JINChun-ying LIMing-chun 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2004年第2期195-199,共5页
The isothermal solubility data of aqueous two-phase system ethanol+water+K 2HPO 4 were determined with the turbidity titration method at 303.2 K. The binodal curves were described by using the Mistry equation very w... The isothermal solubility data of aqueous two-phase system ethanol+water+K 2HPO 4 were determined with the turbidity titration method at 303.2 K. The binodal curves were described by using the Mistry equation very well. An experimental procedure for measuring the liquid-liquid equilibrium data of the aqueous two-phase system was proposed, in which the concentrations of the coexisting phases were determined with the corresponding densities of the solution. The tie lines were satisfactorily described by using the Othmer-Tobias and Bancroft equations. 展开更多
关键词 Aqueous two-phase system SOLUBILITY Turbidity titration LLE ETHANOL K 2HPO 4
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Ionic Liquid-salt Aqueous Two-phase System, a Novel System for theExtraction of Abused Drugs 被引量:4
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作者 She Hong LI Chi Yang HE +2 位作者 Hu Wei LIU Ke An LI Feng LIU 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第8期1074-1076,共3页
A 1-butyl-3-methylimidazolium chloride-salt aqueous two-phase system was studied on extraction of abused drugs. The effects of sorts of salts, temperature, concentration of salt and drugs on system were investigated s... A 1-butyl-3-methylimidazolium chloride-salt aqueous two-phase system was studied on extraction of abused drugs. The effects of sorts of salts, temperature, concentration of salt and drugs on system were investigated systematically. A satisfactory extraction efficiency of 93% was obtained for papaverine while that of morphine was 65%. The extraction mechanism was primarily discussed. 展开更多
关键词 Aqueous two-phase system ionic liquids 1-butyl-3-methylimidazolium chloride extraction abused drugs.
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Measurement of Mass Transfer Coefficients and Their Modelling of Continuous Counter-Current Aqueous Two-Phase System in a Packed Extraction Column 被引量:1
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作者 阎恭喜 朱自强 +1 位作者 关怡新 韩兆熊 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 1999年第3期196-204,共9页
Overall dispersed side volumetric mass transfer coefficients for protein and amino acids were measured in continuous countercurrent PEG4000/KHP aqueous two-phase systems in a 57mm I.D. packed extraction column. A mode... Overall dispersed side volumetric mass transfer coefficients for protein and amino acids were measured in continuous countercurrent PEG4000/KHP aqueous two-phase systems in a 57mm I.D. packed extraction column. A model for overall dispersed side volumetric mass transfer coefficients was derived by describing the motion of the drops based upon Navier-Stokes equation combined with the relationship between mass transfer coefficients and the drop velocity. The model provides good predictions and can be successfully used in aqueous two-phase extraction. The average relative deviation between calculated values and experimental data ranges from 8% to 14%. 展开更多
关键词 aqueous two-phase system continuous countercurrent extraction mass transfer coefficient MODEL packed column
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Measurement and Correlation of Partition Coefficients of Baicalin in EOPO/Salt Aqueous Two-Phase Systems 被引量:1
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作者 李伟 朱自强 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2002年第6期666-669,共4页
The partition coefficients of baicalin were measured in ethylene oxide and propylene oxide (EOPO)/salt aqueous two-phase systems at 298.15K. It was found that most of baicalin partitioned into EOPO-rich phase. The par... The partition coefficients of baicalin were measured in ethylene oxide and propylene oxide (EOPO)/salt aqueous two-phase systems at 298.15K. It was found that most of baicalin partitioned into EOPO-rich phase. The partition coefficients of baicalin varied from 10 to 120. The effect of various factors, including tie-line length, salt composition, molecular weight of EOPO, and solution pH, on the partition behavior was investigated in EOPO/salt systems. Furthermore the partition coefficients of baicalin were correlated using the modified Diamond-Hsu model. Good agreement with experimental data is obtained. The average relative deviations are less than 5.0%. 展开更多
关键词 aqueous two-phase system ethylene oxide and proplene oxide partition coefficient BAICALIN
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Partitioning and purification of extracellular β-1,3-1,4-glucanase in aqueous two-phase systems 被引量:1
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作者 何国庆 张秀艳 +2 位作者 汤兴俊 陈启和 阮辉 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第8期825-831,共7页
The partition behaviors of β-1,3-1,4-glucanase, α-amylase and neutral proteases from clarified and whole fermentation broths of Bacillus subtilis ZJF-1A5 were investigated. An aqueous two-phase system (polyethylene... The partition behaviors of β-1,3-1,4-glucanase, α-amylase and neutral proteases from clarified and whole fermentation broths of Bacillus subtilis ZJF-1A5 were investigated. An aqueous two-phase system (polyethylene glycol (PEG)/MgSO4) was examined with regard to the effects of PEG molecular weight (MW) and concentration, MgSO4 concentration, pH and NaC1 concentration on enzyme partition and extraction. The MW and concentration of PEG were found to have significant effects on enzyme partition and extraction with low MW PEG showing the greatest benefit in the partition and extraction of β-glucanase with the PEG/MgSO4 system. MgSO4 concentration influenced the partition and extraction of β-glucanase significantly, pH had little effect on β-glucanase or proteases partition but affected a-amylase partition when pH was over 7.0. The addition of NaCl had little effect on the partition behavior of β-glucanase but had very significant effects on the partitioning of α-amylase and on the neutral proteases. The partition behaviors of β-glucanase, α-amylase and proteases in whole broth were also investigated and results were similar to those obtained with clarified fermentation broth. A two-step process for purifying β-glucanase was developed, which achieved β-glucanase recovery of 65.3% and specific activity of 14027 U/mg, 6.6 times improvement over the whole broth. 展开更多
关键词 Aqueous two-phase system PARTITION Β-1 3-1 4-GLUCANASE
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Copper(Ⅰ)-catalyzed Synthesis of Aryl, Heteroaryl and Vinyl Sulfides in Aqueous Two-phase System
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作者 WANG Li-jin YUAN Yan-qin GUO Sheng-rong 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2011年第3期417-421,共5页
A fast and convenient C―S bond formation reaction was achieved catalyzed by CuI/L-proline in aqueous two-phase system providing a simple method for synthesis of aryl sulfides in good yields in short time.
关键词 C―S cross-coupling reaction CUI THIOL Aryl halide Aqueous two-phase system
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Efficient Separation of Butyric Acid by an Aqueous Two-phase System with Calcium Chloride 被引量:4
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作者 吴丹 陈灏 +3 位作者 江凌 蔡谨 徐志南 岑沛霖 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2010年第4期533-537,共5页
One of the bottlenecks for bioproduction of butyric acid as bulk chemical is the difficulty in separating butyric acid from the fermentation broth,compared with the petroleum-based chemical synthesis method.In the pre... One of the bottlenecks for bioproduction of butyric acid as bulk chemical is the difficulty in separating butyric acid from the fermentation broth,compared with the petroleum-based chemical synthesis method.In the present work,a novel separation methodology was developed based on an aqueous two-phase system with inor-ganic salts.Calcium chloride was screened out for effective separation of butyric acid from butyric acid-water-salt systems.Within appropriate concentration range of butyric acid and salt,butyric acid was enriched in the upper phase and most of calcium ions remained in the lower phase.This"salting out"effect is very efficient to separate butyric acid from the simulated butyrate fermentation broth,which consists of butyric acid and acetic acid with concentration ratio of 4︰1,so that the final ratio of butyric acid/acetic acid in the upper phase is improved to 9.87. The aqueous two-phase system was used to separate butyric acid from the actual fermentation broth with satisfac-tory result. 展开更多
关键词 butyric acid SEPARATION aqueous two-phase partition salting out effect calcium chloride
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Efficient Production of Enantiopure (S)-Glycidyl Phenyl Ether by an Epoxide Hydrolase from Bacillus megaterium ECU1001 in an Organic-Aqueous Two-Phase System 被引量:4
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作者 宫鹏飞 许建和 《催化学报》 SCIE CAS CSCD 北大核心 2002年第4期290-300,共11页
关键词 (S)-缩水甘油苯基醚 有机-水两相系统 巨大芽孢杆菌 ECU1001 环氧水解酶
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Study on aqueous two-phase systems of the mixture SDS/CTAB surfactants 被引量:1
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作者 LIYing CHENYan-ming +1 位作者 ZHAOKong-shuang TakumiHIKIDA 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2004年第2期282-284,共3页
The phenomenon of two dilute aqueous phases composed of sodium dodecylsulfate(SDS) and cetyl trimethyl ammonium bromide(CTAB) was investigated under various conditions such as concentrations and molar ratios of the tw... The phenomenon of two dilute aqueous phases composed of sodium dodecylsulfate(SDS) and cetyl trimethyl ammonium bromide(CTAB) was investigated under various conditions such as concentrations and molar ratios of the two surfactants, the addition of sodium chloride and temperature. Vesicles formation was found in the both phases by TEM image. 展开更多
关键词 aqueous surfactant two-phase(ASTP) cationic-anionic surfactants vesicles
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EXTRACTION OF INTERFERON-γ USING PEG/K_3PO_4 AQUEOUS TWO-PHASE SYSTEM
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作者 沈忠耀 丛梅 杨基础 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 1993年第3期48-51,共4页
The aqueous two-phase system of PEG-4000/K<sub>3</sub>PO<sub>4</sub> is selected to extract interferon-γ(γ-IFN)from the broth of homogenized E.coil cells,which were recombined genetically a... The aqueous two-phase system of PEG-4000/K<sub>3</sub>PO<sub>4</sub> is selected to extract interferon-γ(γ-IFN)from the broth of homogenized E.coil cells,which were recombined genetically and werefermented in high expressivity.The effects of pH value and the concentrations of PEG and phos-phate on the partition behavious of γ-IFN and contaminating proteins have been investigated.Theresults show that the pH value is the most influential factor effecting the extraction yield of γ-IFN.Under a suitable condition,an effective separation with high yield of γ-IFN can be achieved. 展开更多
关键词 AQUEOUS two-phase EXTRACTION INTERFERON-Γ
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Extraction of Tetracycline via Ionic Liquid Two-phase System
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作者 MA Chun-hong WANG Liang +4 位作者 YAN Yong-sheng CHE Guang-bo YIN Yan-su WANG Ren-zhang LI Dong-ying 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2009年第6期832-835,共4页
A new method to extract and separate tetracycline(TC) in a new aqueous tyro-phase system(ATPS) consisting of hydrophilic [Bmim]BF4 and NaH2PO4 was investigated. The effects of the amount of salt, ionic liquid as w... A new method to extract and separate tetracycline(TC) in a new aqueous tyro-phase system(ATPS) consisting of hydrophilic [Bmim]BF4 and NaH2PO4 was investigated. The effects of the amount of salt, ionic liquid as well as tetracycline on extraction efficiency were studied systematically. The results show that ionic liquid-ATPS exhibited the highest extraction efficiency higher than 90%. When the concentration of NaH2PO4 was 33.3%--38.5% (mass fraction), the amounts of ionic liquid and TC were 1.0--2.0 mL and 1.8 mL, respectively, and ionic liquid could be re-used. 展开更多
关键词 Ionic liquid Aqueous two-phase TETRACYCLINE Extraction separation
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The Effect of pH Difference Between Two Phases on the Partition of Lysozyme in Aqueous Two-Phase System
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作者 Yan XU Jun LU De Qian LI (Department of Chemical Engineering, Hebei Institute of Technology, Tangshan 063009 Laboratory of Rare Earth Chemistry and Physics, Changchun institute of Applied Chemistry. Chinese Academy of Sciences, Changchun 130022) 《Chinese Chemical Letters》 SCIE CAS CSCD 2000年第5期453-454,共2页
In the investigation of effect of KSCN on the partitioning of lysozyme in PEG2000/ammonium sulfate aqueous two-phase system, it was found that the KSCN could alter the pH difference between the two phases. and thus af... In the investigation of effect of KSCN on the partitioning of lysozyme in PEG2000/ammonium sulfate aqueous two-phase system, it was found that the KSCN could alter the pH difference between the two phases. and thus affect the partition of lysozyme. The relationship between partition coefficients of lysozyme and pH differences between two phases was discussed. 展开更多
关键词 aqueous two-phase polyethylene glycol 2000 LYSOZYME
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Effects of Hydrophobicity of Ethylene Oxide-Propylene Oxide Copolymers on Phase Diagrams of Aqueous Two-Phase Systems and Partition Behaviors of Cephalexin and 7-Aminodeacetoxicephalos-poranic Acid
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作者 朱建航 魏东芝 曹学君 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2002年第2期240-243,共4页
A series of ethylene oxide (EO)-propylene oxide (PO) randomco-polymers (EOPO) were used to form aqueous two-phase systems (ATPS)with ammonium sulfate. Effects of EOPO's properties on the phaseseparation behaviors ... A series of ethylene oxide (EO)-propylene oxide (PO) randomco-polymers (EOPO) were used to form aqueous two-phase systems (ATPS)with ammonium sulfate. Effects of EOPO's properties on the phaseseparation behaviors and on the partition of cephalexin and7-aminodesacetoxicephalosporanic acid (7-ADCA) in ATPS wereinvestigated. Both the molar mass and molar ratio of EO to PO of EOPOcould greatly influence partition behaviors of cephalexin and 7-ADCAas well as the binodal curve of ATPS. 展开更多
关键词 ethylene oxide (EO) and propylene oxide (PO) random co-polymer (EOPO) aqueous two-phase sys-tems partition coefficient phase diagram
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Separation/Analysis Rhodamine B by Anion Surfactant/Ionic Liquid Aqueous Two-Phase Systems Coupled with Ultraviolet Spectrometry
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作者 Jun Wu Hao Fu Xiashi Zhu 《Detection》 2014年第3期17-25,共9页
A novel method for the separation/analysis rhodamine B has been described. The ionic liquid (1-tetradecyl-3-methylimidazolium bromide)/anion surfactant (sodium dodecyl sulfate)/NaCl two-phase systems (ATPSs) is presen... A novel method for the separation/analysis rhodamine B has been described. The ionic liquid (1-tetradecyl-3-methylimidazolium bromide)/anion surfactant (sodium dodecyl sulfate)/NaCl two-phase systems (ATPSs) is presented as a simple, rapid and effective sample pretreatment technique coupled with ultraviolet spectrometry for analysis rhodamine B in soft drink. The effects of parameters on the ATPSs extraction of rhodamine B such as amount of surfactant, ionic liquid and salt, pH, temperature, stabilization and centrifugal time have been studied in details. Under the optimized conditions, the linear range of calibration curve for rhodamine B was 0.05 - 7.0 μg·mL-1 and the detection limit was 3.2 ng·mL-1. The phase equilibrium and the mechanism of phase separation for ATPSs have been discussed. This method has been applied to the determination of rhodamine B in soft drink. 展开更多
关键词 RHODAMINE B Sodium Dodecyl SULFATE 1-Tetradecyl-3-methylimidazolium BROMIDE The AQUEOUS two-phase Systems (ATPSs)
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Mixture temperature prediction of waxy oil–water two-phase system flowing near wax appearance temperature
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作者 Ali Piroozian Muhammad A. Manan +4 位作者 Issham Ismail Rahmat Mohsin Ali Esfandyari Bayat Mac DarlingtonUche Onuoha Mahmoud Hemmati 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2016年第6期795-802,共8页
Temperature sensitivity of waxy crude oils makes it difficult to study their flow behaviour in the presence of water especially near their wax appearance temperature (WAT). In this study a method was proposed and im... Temperature sensitivity of waxy crude oils makes it difficult to study their flow behaviour in the presence of water especially near their wax appearance temperature (WAT). In this study a method was proposed and implemented to mitigate such difficulties which was applied in predicting mixture temperatures (Tin) of a typical Malaysian waxy crude oil and water flow in a horizontal pipe. To this end, two analytical models were derived firstly from calorimetry equation which based on developed two correlations for defining crude oil heat capacity actualized from the existed specific heat capacities of crude oils. The models were then applied for a set of experiments to reach the defined three predetermined Tm (26 ℃, 28 ℃ and 30 ℃). The comparison between the predicted mixture temperatures (Tin,1 and Tin,2) from the two models and the experimental results displayed acceptable absolute average errors (0.80% 0.62%, 0.53% for model 1; 0,74%, 0.54%, 0.52% for model 2). Moreover, the average errors for both models are in the range of standard error limits (4-0,75Z) according to ASTM E230. Conclusively, the proposed model showed the ease of obtaining mixture temperatures close to WAT as predetermined with accuracy of ± 0.5 ℃approximately for over 84% of the examined cases. The method is seen as a practical reference point to further study the flow behaviour of waxy crudes in oil-water two-phase flow system near sensitive temperatures. 展开更多
关键词 Mixture temperature predictionAnalytical modelWaxy oil-water two-phase flowWax appearance temperature
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Emerging delivery systems based on aqueous two-phase systems:A review
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作者 Yaowen Zhang Yankun Luo +4 位作者 Jingqi Zhao Wenzhuo Zheng Jun Zhan Huaping Zheng Feng Luo 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2024年第1期110-132,共23页
The aqueous two-phase system(ATPS)is an all-aqueous system fabricated from two immiscible aqueous phases.It is spontaneously assembled through physical liquid-liquid phase separation(LLPS)and can create suitable templ... The aqueous two-phase system(ATPS)is an all-aqueous system fabricated from two immiscible aqueous phases.It is spontaneously assembled through physical liquid-liquid phase separation(LLPS)and can create suitable templates like the multicompartment of the intracellular environment.Delicate structures containing multiple compartments make it possible to endow materials with advanced functions.Due to the properties of ATPSs,ATPS-based drug delivery systems exhibit excellent biocompatibility,extraordinary loading efficiency,and intelligently controlled content release,which are particularly advantageous for delivering drugs in vivo.Therefore,we will systematically review and evaluate ATPSs as an ideal drug delivery system.Based on the basic mechanisms and influencing factors in forming ATPSs,the transformation of ATPSs into valuable biomaterials is described.Afterward,we concentrate on the most recent cutting-edge research on ATPS-based delivery systems.Finally,the potential for further collaborations between ATPS-based drug-carrying biomaterials and disease diagnosis and treatment is also explored. 展开更多
关键词 Aqueous two-phase systems Drug delivery systems BIOMATERIALS MICROCAPSULES MICROPARTICLES Cell therapy Cancer treatment Organ regeneration
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Evaluation of frictional pressure drop correlations for air-water and air-oil two-phase flow in pipeline-riser system
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作者 Nai-Liang Li Bin Chen 《Petroleum Science》 SCIE EI CAS CSCD 2024年第2期1305-1319,共15页
Accurate prediction of the frictional pressure drop is important for the design and operation of subsea oil and gas transporting system considering the length of the pipeline. The applicability of the correlations to ... Accurate prediction of the frictional pressure drop is important for the design and operation of subsea oil and gas transporting system considering the length of the pipeline. The applicability of the correlations to pipeline-riser flow needs evaluation since the flow condition in pipeline-riser is quite different from the original data where they were derived from. In the present study, a comprehensive evaluation of 24prevailing correlation in predicting frictional pressure drop is carried out based on experimentally measured data of air-water and air-oil two-phase flows in pipeline-riser. Experiments are performed in a system having different configuration of pipeline-riser with the inclination of the downcomer varied from-2°to-5°to investigated the effect of the elbow on the frictional pressure drop in the riser. The inlet gas velocity ranges from 0.03 to 6.2 m/s, and liquid velocity varies from 0.02 to 1.3 m/s. A total of885 experimental data points including 782 on air-water flows and 103 on air-oil flows are obtained and used to access the prediction ability of the correlations. Comparison of the predicted results with the measured data indicate that a majority of the investigated correlations under-predict the pressure drop on severe slugging. The result of this study highlights the requirement of new method considering the effect of pipe layout on the frictional pressure drop. 展开更多
关键词 Frictional pressure drop Pipeline-riser Gas-liquid two-phase flow Severe slugging CORRELATION
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