Two carbazole-based polymerization initiators possessing blue fluorescence emission have been synthesized via Wittig reaction in the solid phase at room temperature.Two-photon excited fluorescence(TPEF) spectra for th...Two carbazole-based polymerization initiators possessing blue fluorescence emission have been synthesized via Wittig reaction in the solid phase at room temperature.Two-photon excited fluorescence(TPEF) spectra for them were investigated under 800 nm fs laser pulse and two-photon absorption cross sections were determined by the Z-scan technique.Then two-photon initiating polymerization(TPIP) microfabrication experiments were successfully carried out.Three-dimensional lattice and artificial defects were gained,indicating that they were viable candidates for the two-photon polymerization initiator in practical application of microfabrication.展开更多
The high-precision integration of three-dimensional(3D)microoptical components into microfluidics in a customizable manner is crucial for optical sensing,fluorescence analysis,and cell detection in optofluidic applica...The high-precision integration of three-dimensional(3D)microoptical components into microfluidics in a customizable manner is crucial for optical sensing,fluorescence analysis,and cell detection in optofluidic applications;however,it remains challenging for current microfabrication technologies.This paper reports the in-channel integration of flexible two-dimensional(2D)and 3D polymer microoptical devices into glass microfluidics by developing a novel technique:flat scaffold-supported hybrid femtosecond laser microfabrication(FSS-HFLM).The scaffold with an optimal thickness of 1–5 μm is fabricated on the lower internal surface of a microfluidic channel to improve the integration of high-precision microoptical devices on the scaffold by eliminating any undulated internal channel surface caused by wet etching.As a proof of demonstration,two types of typical microoptical devices,namely,2D Fresnel zone plates(FZPs)and 3D refractive microlens arrays(MLAs),are integrated.These devices exhibit multicolor focal spots,elongated(>three times)focal length and imaging of the characters‘RIKEN’in a liquid channel.The resulting optofluidic chips are further used for coupling-free white-light cell counting with a success rate as high as 93%.An optofluidic system with two MLAs and a W-filter is also designed and fabricated for more advanced cell filtering/counting applications.展开更多
For future micro-and nanotechnologies,the manufacturing of miniaturized,functionalized,and integrated devices is indispensable.In this paper,an assembly technique based on a bottom-up strategy that enables the manufac...For future micro-and nanotechnologies,the manufacturing of miniaturized,functionalized,and integrated devices is indispensable.In this paper,an assembly technique based on a bottom-up strategy that enables the manufacturing of complex microsystems using only optical methods is presented.A screw connection is transferred to the micrometer range and used to assemble screwand nut-shaped microcomponents.Micro-stereolithography is performed by means of two-photon polymerization,and microstructures are fabricated and subsequently trapped,moved,and screwed together using optical forces in a holographic optical tweezer set-up.The design and construction of interlocking microcomponents and the verification of a stable and releasable joint form the main focus of this paper.The assembly technique is also applied to a microfluidic system to enable the pumping or intermixing of fluids on a microfluidic chip.This strategy not only enables the assembly of microcomponents but also the combination of different materials and features to form complex hybrid microsystems.展开更多
基金Supported by the National Natural Science Foundation of China (Grant Nos. 50532030, 20771001 & 50703001)Team for Scientific Innovation of Anhui Province (Grant No. 2006KJ007TD)
文摘Two carbazole-based polymerization initiators possessing blue fluorescence emission have been synthesized via Wittig reaction in the solid phase at room temperature.Two-photon excited fluorescence(TPEF) spectra for them were investigated under 800 nm fs laser pulse and two-photon absorption cross sections were determined by the Z-scan technique.Then two-photon initiating polymerization(TPIP) microfabrication experiments were successfully carried out.Three-dimensional lattice and artificial defects were gained,indicating that they were viable candidates for the two-photon polymerization initiator in practical application of microfabrication.
基金This work was supported by JSPS KAKENHI Grant Number 25286038.
文摘The high-precision integration of three-dimensional(3D)microoptical components into microfluidics in a customizable manner is crucial for optical sensing,fluorescence analysis,and cell detection in optofluidic applications;however,it remains challenging for current microfabrication technologies.This paper reports the in-channel integration of flexible two-dimensional(2D)and 3D polymer microoptical devices into glass microfluidics by developing a novel technique:flat scaffold-supported hybrid femtosecond laser microfabrication(FSS-HFLM).The scaffold with an optimal thickness of 1–5 μm is fabricated on the lower internal surface of a microfluidic channel to improve the integration of high-precision microoptical devices on the scaffold by eliminating any undulated internal channel surface caused by wet etching.As a proof of demonstration,two types of typical microoptical devices,namely,2D Fresnel zone plates(FZPs)and 3D refractive microlens arrays(MLAs),are integrated.These devices exhibit multicolor focal spots,elongated(>three times)focal length and imaging of the characters‘RIKEN’in a liquid channel.The resulting optofluidic chips are further used for coupling-free white-light cell counting with a success rate as high as 93%.An optofluidic system with two MLAs and a W-filter is also designed and fabricated for more advanced cell filtering/counting applications.
基金We thank the German Research Foundation DFG(Deutsche Forschungsgesellschaft)for their generous support within the Reinhardt Koselleck project(OS 188/28-1).
文摘For future micro-and nanotechnologies,the manufacturing of miniaturized,functionalized,and integrated devices is indispensable.In this paper,an assembly technique based on a bottom-up strategy that enables the manufacturing of complex microsystems using only optical methods is presented.A screw connection is transferred to the micrometer range and used to assemble screwand nut-shaped microcomponents.Micro-stereolithography is performed by means of two-photon polymerization,and microstructures are fabricated and subsequently trapped,moved,and screwed together using optical forces in a holographic optical tweezer set-up.The design and construction of interlocking microcomponents and the verification of a stable and releasable joint form the main focus of this paper.The assembly technique is also applied to a microfluidic system to enable the pumping or intermixing of fluids on a microfluidic chip.This strategy not only enables the assembly of microcomponents but also the combination of different materials and features to form complex hybrid microsystems.
