Lentinula edodes extract inhibits matrix metalloproteinase expression and increases typeⅠprocollagen expression via the p38 MAPK/c-Fos signaling pathway in ultraviolet A and B-irradiated HaCaT keratinocytes

Objective:To determine the effect of Lentinula edodes extract on ultraviolet(UV)A and UVB-induced changes in matrix metalloproteinase(MMP)and type I procollagen expression using human immortalized HaCaT keratinocytes.Methods:Lentinula edodes ethanol extract(LEE)was obtained by extraction with 80%ethanol for 4 h at 80℃.Effect of LEE on UVinduced alteration on the expression and production of MMPs and type I procollagen in keratinocytes was investigated using ELISA,RT-PCR,and Western blotting assay.To determine the underlying mechanism of LEE-mediated effects,mitogen-activated protein kinase(MAPK)and activator protein 1 signaling pathways were analysed by Western blotting assay.Results:LEE significantly inhibited the expression of MMP-1 and MMP-9 and increased the expression of type I procollagen in UVA and UVB-irradiated HaCaT keratinocytes.The phosphorylation levels of p38 were significantly inhibited by LEE whereas it did not affect c-Jun N-terminal kinase and extracellular signal-regulated kinase phosphorylation.Suppression of p38 phosphorylation was also accompanied by downregulation of UVA and UVB-induced increase in c-Fos.Conclusions:LEE effectively inhibits the expression of MMP-1 and MMP-9 and increases type I procollagen production through the p38 MAPK/c-Fos signaling pathway in UVA and UVB-irradiated HaCaT keratinocytes.This findings suggest that Lentinula edodes may be developed as a cosmetic material to suppress UV exposuremediated skin aging.

TypeⅠ多元零膨胀指数威布尔分布及其性质研究

首先,本文提出一种新的寿命分布——零膨胀指数威布尔(ZEW)分布,并将单变量ZEW分布推广到TypeⅠ多元零膨胀指数威布尔(MZEW(Ι))分布,以用于对包含过多零的多元计数数据进行建模研究;其次,研究了MZEW(Ι)分布的联合概率质量函数、累积分布函数、矩母函数、边缘分布、极大似然估计和Fisher信息阵。

Protein Flexibility and Multiple Docking in Ligand Docking and Virtual Screening to the BRAF(TypeⅠ1/2)Inhibitors

BRAF has been recognized as a promising target for cancer therapy. A number of crystal structures have been published. Molecular docking is one of the most effective techniques in the field of computer-aided drug design（CADD）. Appropriate protein conformation and docking method are essential for the successful virtual screening experiments. One approach considering protein flexibility and multiple docking methods was proposed in this study. Six DFG-in/αC-helix-out crystal structures of BRAF, three docking programs（Glide, GOLD and Ligand Fit） and 12 scoring functions were applied for the best combination by judging from the results of pose prediction and retrospective virtual screening（VS）. The most accurate results（mean RMSD of about 0.6 A） of pose prediction were obtained with two complex structures（PDB： 3 C4 C and 3 SKC） using Glide SP. From the retrospective VS, the most active compounds were identified by using the complex structure of 3 SKC, indicated by a ROC/AUC score of 0.998 and an EF of 20.6 at 5% of the database screen with Glide-SP. On the whole, PDB 3 SKC could achieve a higher rate of correct reproduction, a better enrichment and more diverse compounds. A comparison of 3 SKC and the other X-ray crystal structures led to a rationale for the docking results. PDB 3 SKC could achieve a broad range of sulfonamide substitutions through an expanded hydrophobic pocket formed by a further shift of the αC-helix. Our study emphasized the necessity and significance of protein flexibility and scoring functions in both ligand docking and virtual screening.

Clinical Efficacy of Minimally Invasive Subpial Tonsillectomy(MIST)for Treatment of Chiari Malformation(TypeⅠ)with Syringomyelia

Background To investigate the clinical efficacy of minimally invasive subpial tonsillectomy(MIST)in the treatment of Chiari malformation(type I)with syringomyelia.Methods A total of 209 Chiari malformation(type I)patients with syringomyelia were studied.The patients were grouped based on the syrinx diameter changes:complete disappearance group(48 patients),obvious shrinkage group(147 patients),and non-obvious shrinkage group(14 patients).The Chicago Chiari Outcome Scale(CCOS)was used to compare clinical data of the three groups of patients before treatment.The correlations between disease duration and syrinx diameter changes as well as post-treatment clinical symptoms were analyzed.The related factors of efficacy were analyzed.Results Age and disease duration were the oldest/longest in the non-obvious shrinkage group,and the youngest/shortest in the complete disappearance group(P<0.05).The maximum diameter reduction of syrinx was the greatest in the complete disappearance group,and the smallest in the non-obvious shrinkage group(P<0.05).The proportions of patients with hypoesthesia,limb weakness,and muscle atrophy were the largest in the non-obvious shrinkage group,and the smallest in the complete disappearance group(P<0.05).The CCOS score were the highest in the complete disappearance group,and the lowest in the non-obvious shrinkage group(P<0.05).There were statistically significant(P<0.05)negative correlations between disease duration and maximum diameter reduction of syrinx,CCOS pain score,CCOS non-pain score,CCOS functionality score,and CCOS complication score,disease duration and hypoesthesia,limb weakness,muscle atrophy,and sleep apnea.Result of multivariate stepwise regression analysis indicated that age,disease duration,and preoperative syrinx diameter were the risk factors for efficacy(P<0.05).Conclusion For patients with Chiari malformation complicated by syringomyelia,the longer the disease duration,the more difficult it is to achieve syrinx reduction and improve the clinical symptoms.“Minimally invasive subpial tonsillectomy(MIST)and cisterna magna reconstruction”is an improved surgical approach to treat Chiari malformation(cerebellar tonsil herniation).It has the advantages of small incision,less postoperative reaction,and fewer complications,and it emphasizes the reshaping and repair of cerebellar tonsils,reconstruction of cisterna magna,and restoration of cerebrospinal fluid circulation.

组织桥接整合因子1、Ⅰ型胶原蛋白基因、核转运蛋白基因2与三阴性乳腺癌术后复发关系

目的探究组织桥接整合因子1(BIN1)、Ⅰ型胶原蛋白基因(COL1A1)、核转运蛋白基因2(KPNA2)表达与三阴性乳腺癌(TNBC)术后复发关系及联合检测意义。方法选取2019年5月至2020年5月上海市第八人民医院行手术治疗的TNBC病人80例,根据术后2年是否复发分为复发组、未复发组,比较两组临床资料、组织BIN1、COL1A1、KPNA2基因表达,复发的相关影响因素采用单因素与多因素logistic回归分析,组织BIN1、COL1A1、KPNA2基因表达预测术后复发的价值采用受试者操作特征曲线(ROC曲线)分析,组织BIN1、COL1A1、KPNA2基因表达与复发时间关系采用Pearson分析,比较不同组织BIN1、COL1A1、KPNA2基因表达病人无复发生存期(RFS)。结果复发组T分期、N分期与未复发组比较,差异有统计学意义(P<0.05);复发组组织BIN1mRNA表达为0.24±0.07,低于未复发组的0.35±0.10(P<0.05),复发组组织COL1A1mRNA、KPNA2mRNA表达分别为2.07±0.62、2.91±0.84,高于未复发组的1.48±0.43、1.85±0.60(P<0.05);T分期、N分期、COL1A1 mRNA、KPNA2 mRNA均是复发相关独立危险因素,BIN1 mRNA是复发相关独立保护因素(P<0.05);BIN1 mRNA、COL1A1 mRNA、KPNA2 mRNA的AUC分别为0.76、0.80、0.78,三者联合的AUC为0.94;BIN1mRNA与复发时间呈负相关(r=-0.79,P<0.001),COL1A1mRNA、KPNA2mRNA与复发时间呈正相关(r=0.77、0.78,均P<0.001);BIN1mRNA高水平病人RFS长于低水平病人,COL1A1mRNA、KPNA2mRNA高水平病人RFS短于低水平病人(P<0.05)。结论BIN1mRNA、COL1A1mRNA、KPNA2mRNA与TNBC术后复发风险、复发时间有关,联合检测可作为早期预测术后复发的一个可靠方案,为临床治疗提供重要的参考信息。

Chiari畸形Ⅰ型手术预后影响因素分析

目的探讨小脑扁桃体下疝畸形Ⅰ型(CM-Ⅰ)手术预后的相关性因素。方法回顾性分析新疆医科大学第一附属医院神经外科2016-08—2023-01手术治疗的80例CM-Ⅰ患者的临床资料,行芝加哥预后评分(CCOS),分析性别、年龄、高血压、糖尿病、BMI、病程、术前脊髓空洞、术前神经功能缺损、扁桃体凸出程度、术式等因素对患者手术预后的影响,确定CM-Ⅰ手术预后的相关因素。结果年龄(OR=0.015,95%CI:0.001~0.448,P=0.015)、病程(OR=0.020,95%CI:0.001~0.477,P=0.016)、术前脊髓空洞(OR=0.017,95%CI:0.000~0.916,P=0.045)及术式(OR=0.042,95%CI:0.002~0.984,P=0.049)是CM-Ⅰ手术预后的独立影响因素。结论CM-Ⅰ手术预后的相关性因素包括年龄、病程、术前脊髓空洞及术式。

戊二酸血症Ⅰ型病儿5例临床及基因突变分析

目的回顾性分析5例确诊戊二酸血症Ⅰ型病儿临床特点及戊二酰辅酶A脱氢酶(GCDH)基因突变情况,为临床诊治提供参考依据及产前诊断提供借鉴。方法回顾性分析山西省儿童医院2019年1月至2022年12月收治及随访的5例戊二酸血症Ⅰ型病儿临床资料及基因检测结果。结果5例病儿中男4例,女1例,5例病儿就诊年龄分别为8个月26 d、3个月24 d、4个月8 d、10个月25 d、1个月5 d。5例病儿的临床表型各异。3例经新生儿筛查确诊,2例因运动发育落后经遗传代谢病检测确诊,其中1例以抽搐为首发症状,2例出现运动发育落后且1例伴有运动发育倒退。5例病儿血戊二酰肉碱及比值均升高、尿戊二酸水平均增高。基因检测证实5例病儿均为复合杂合突变,以错义突变为主。结论5例病儿中有3例病儿是通过新生儿疾病筛查发现的,新生儿串联质谱筛查可以对于戊二酸血症病儿的早期诊断、早期治疗及预后具有重要的意义;戊二酸血症Ⅰ型病儿临床表现具有明显异质性,对临床可疑的病儿可进行血串联质谱检测、尿气相色谱分析及基因检测,以便进行早期严格的干预治疗及饮食管理,从而改善预后;此外,该研究发现了1个新的GCDH基因突变位点,丰富了GCDH基因的突变谱。

血流导向密网支架桥接治疗神经纤维瘤病Ⅰ型相关儿童颅内动脉瘤一例

儿童颅内动脉瘤在临床上较为少见,其发病机制目前尚不明确,治疗方式也存在诸多争议。作者报道了1例采用2枚血流导向密网支架桥接治疗的神经纤维瘤病Ⅰ型相关的儿童颅内动脉瘤,并结合相关文献进行了分析,希望为探索儿童颅内动脉瘤的治疗提供有益参考。

内质网应激蛋白激酶RNA样ER激酶(PERK)通路对肝星状细胞激活及Ⅰ型胶原蛋白表达的影响

目的探讨内质网应激蛋白激酶RNA样ER激酶(PERK)/真核生物翻译起始因子2α(eIF2α)信号通路对肝星状细胞(HSC)活化的影响。方法收集11例肝穿刺病理提示S1~S4肝纤维化患者和9例肝血管瘤、肝腺瘤患者术后周围正常肝组织病理切片,进一步行组织免疫组化检测PERK、eIF2α、C/EBP环磷酸腺苷反应元件结合转录因子同源蛋白(CHOP)表达情况;使用不同浓度的内质网应激诱导剂毒胡萝卜素(0、125、250、500、1000 nmol/L)作用于人HSC-LX2,使用qRT-PCR检测PERK mRNA及Western Blot检测PERK、肌醇需要酶1(IRE1)、激活转录因子6(ATF6)、CHOP及α平滑肌肌动蛋白(α-SMA)表达水平。使用慢病毒转染构建PERK稳定过表达LX-2组及对照组,并通过qRT-PCR检测PERK、eIF2α、α-SMA mRNA,Western Blot检测PERK、p-eIF2α、α-SMA蛋白表达,免疫荧光检测胶Ⅰ型原蛋白(COL1A1)表达。符合正态分布的计量资料两组间比较采用成组t检验,多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验;不符合正态分布的计数资料,两组间比较采用Mann-Whitney U秩和检验。结果与正常肝组织相比,肝纤维化患者肝组织中PERK、eIF2α及CHOP表达升高,差异均有统计学意义(Z=−3.56、t=−5.75、Z=−3.52,P值均<0.001)。不同浓度毒胡萝卜素作用后,与溶媒组相比,内质网相关蛋白PERK、CHOP、IRE1、ATF6及α-SMA蛋白表达显著升高(P值均<0.05)。与对照空载慢病毒组相比,PERK稳定过表达组PERK mRNA、eIF2αmRNA、α-SMA mRNA表达及PERK、p-eIF2α、α-SMA蛋白表达明显升高(P值均<0.05)。细胞免疫荧光结果提示,PERK过表达组COL1A1表达升高(P<0.05)。结论PERK过表达可诱导LX-2细胞α-SMA、胶原蛋白COL1A1表达,提示PERK信号通路可能是HSC活化的重要机制之一。

拉罗尼酶治疗黏多糖贮积症Ⅰ型药品潜在不良事件的挖掘与分析

目的为临床合理安全的使用拉罗尼酶提供参考依据。方法通过对美国FDA不良事件报告系统(FAERS)进行拉罗尼酶的药品不良事件的风险信号挖掘,采用比值比法(ROR)和综合标准法(MHRA)对所挖掘出的拉罗尼酶不良事件风险信号进行整理与分析。结果共挖掘出拉罗尼酶不良事件2795例,男性占1272例(45.5%)与女性1242例(44.4%),男女比例基本持平;数据上报人员主要是医护工作者(1455例,占52.1%)和消费者(1314例,占47.0%);AE报告中病人年龄2岁以下445例(12.3%),2~17岁1191例(42.6%),>17~64岁638例(22.8%);全部AE事件中死亡病例526例(18.9%)。共挖掘不良事件信号61个,映射15个系统器官分类(SOC)。发热、肺炎、呕吐、呼吸困难、咳嗽、不适、荨麻疹及呼吸衰竭等为主要首选术语(PT)信号。结论基于FARES数据库的挖掘分析可为临床合理安全的使用拉罗尼酶提供依据;拉罗尼酶应用的安全性及有效性仍需要进行长期研究,以确定其对生存和生活质量的全面影响。

重组Ⅰ型人源化胶原蛋白“福活因子”的生物合成及其结构表征研究

目的:筛选获得含胶原蛋白保守序列hCol.1基因的重组人源化Ⅰ型胶原蛋白高效表达基因工程菌和高产创新融合蛋白,并通过检测其理化特征验证表达产物。方法:将His6-SUMO-hCol.1重组基因大肠杆菌高密度发酵后经镍亲和层析和离子交换层析两步纯化浓缩获得重组蛋白,检测理化测试特征。结果:测序证明克隆基因与hCol.1序列高度一致,Blast比对蛋白序列与人Ⅰ型胶原蛋白高度同源,折叠结构与人Ⅲ型胶原蛋白高度类似。表观分子量55~60kD,产量36.4mg/L,纯度>95%。等电点检测、氨基酸组成和圆二色谱等实验结合高级结构预测分析,证明其理化性状与蛋白结构符合胶原蛋白特征。结论:成功将人Ⅰ型胶原蛋白保守功能结构域hCol.1与SUMO等肽段重组融合表达并命名为福活因子(FHTFF),筛选出基因工程菌pET21a(+)-FHTFF/BL21,纯化产物“福活因子”的理化特性、序列和结构与人Ⅰ、Ⅲ型胶原蛋白肽段高度相似,属于重组人源化胶原蛋白新生物材料。

Effects of Icariin on Expression of OPN mRNA and Type ⅠCollagen in Rat Osteoblasts in Vitro

To study the effects of Icariin on expression of osteopontin （OPN） mRNA and type Ⅰ collagen in rat osteoblasts in vitro and to explore its possible mechanisms in preventing osteoporosis. OB was isolated from calvaria of new-born new-born fetal Sprague-Dawley （SD） rats by means of modified sequential collagenase digestion and incubated in MEM medium and the cell morphology was observed under inverted phase contrast microscope, OB was identified by alkaline phosphatase （ALP） staining. Different concentration （0.1μg/mL, 1.0 μg/mL, 10 μ/mL） of Icariin was added to the OB and incubated. The effect of Icariin on the proliferation and osteogenesis of OB was monitored by MTT analysis. The expression of type l collagen was estimated with immunohistochemistry techniques. The expression levels of mRNA of OPN in the cells in every group were examined by reverse-transcriptase ploymerase chain reaction （RT-PCR）. The expression of OPN mRNA and type Ⅰ collagen was strengthened gradually with the increase of Icariin concentration and peaked with 10 μg/mL Icariin on the 5th day. Icariin could significantly promote the expression of OPN mRNA and type Ⅰ collagen in rat osteoblasts in vitro. The levels of expression of OPN mRNA and type Ⅰ collagen were changed with different concentration of Icariin. Icariin could effectively prevent and treat osteoporosis and promote the bone formation.

Toward a better understanding of typeⅠinterferonopathies:a brief summary,update and beyond

Backgrounds TypeⅠinterferonopathy is a group of autoinflammatory disorders associated with prominent enhanced typeⅠinterferon signaling.The mechanisms are complex,and the clinical phenotypes are diverse.This review briefly summarized the recent progresses of typeⅠinterferonopathy focusing on the clinical and molecular features,pathogeneses,diagnoses and potential therapies.Data sources Original research articles and literature reviews published in PubMed-indexed journals.Results TypeⅠinterferonopathies include Aicardi-Goutières syndrome,spondyloenchondro-dysplasia with immune dysregulation,stimulator of interferon genes-associated vasculopathy with onset in infancy,X-linked reticulate pigmentary disorder,ubiquitin-specific peptidase 18 deficiency,chronic atypical neutrophilic dermatitis with lipodystrophy,and Singleton-Merten syndrome originally.Other disorders including interferon-stimulated gene 15 deficiency and DNAseⅡdeficiency are believed to be interferonopathies as well.Intracranial calcification,skin vasculopathy,interstitial lung disease,failure to thrive,skeletal development problems and autoimmune features are common.Abnormal responses to nucleic acid stimuli and defective regulation of protein degradation are main mechanisms in disease pathogenesis.First generation Janus kinase inhibitors including baricitinib,tofacitinib and ruxolitinib are useful for disease control.Reverse transcriptase inhibitors seem to be another option for Aicardi-Goutières syndrome.Conclusions Tremendous progress has been made for the discovery of typeⅠinterferonopathies and responsible genes.Janus kinase inhibitors and other agents have potential therapeutic roles.Future basic,translational and clinical studies towards disease monitoring and powerful therapies are warranted.

Type Ⅰ kerogen-rich oil shale from the Democratic Republic of the Congo: mineralogical description and pyrolysis kinetics

The Democratic Republic of the Congo holds important reserves of oil shale which is still under geological status.Herein,the characterization and pyrolysis kinetics of typeⅠkerogen-rich oil shale of the western Central Kongo(CK)were investigated.X-ray diffraction,Fourier-transform infrared spectroscopy and thermal analysis(TG/DTA)showed that CK oil shale exhibits a siliceous mineral matrix with a consistent organic matter rich in aliphatic chains.The pyrolysis behavior of kerogen revealed the presence of a single mass loss between 300 and 550°C,estimated at 12.5%and attributed to the oil production stage.Non-isothermal kinetics was performed by determining the activation energy using the iterative isoconversional model-free methods and exhibits a constant value with E=211.5±4.7 kJ mol.1.The most probable kinetic model describing the kerogen pyrolysis mechanism was obtained using the Coats–Redfern and Arrhenius plot methods.The results showed a unique kinetic triplet confirming the nature of kerogen,predominantly typeⅠand reinforcing the previously reported geochemical characteristics of the CK oil shale.Besides,the calculation of thermodynamic parameters(ΔH~*,ΔS~*andΔG~*)corresponding to the pyrolysis of typeⅠkerogen revealed that the process is non-spontaneous,in agreement with DTA experiments.

Neurofibromatosis type Ⅰ caused by a splicing mutation in NF1 using targeted next generation sequencing

Objective Neurofibromatosis typeⅠ(NF1)is an autosomal dominant disorder which is caused by loss-of-function mutations in neurofibromin 1 gene(NF1).Clinically,NF1 mainly manifests several typical features,such as multiple neurofibromas and café-au-lait spots,as well as axillary freckling and Lisch nodules in iris.The aim of the current study is to identification a splicing mutation and genotype-phenotype correlation.

CD47 decline in pancreatic islet cells promotes macrophage-mediated phagocytosis in type Ⅰ diabetes

BACKGROUND Type Ⅰ diabetes(T1D)is characterized by insulin loss caused by inflammatory cells that excessively infiltrate and destroy the pancreas,resulting in dysregulation of tissue homeostasis,mechanobiological properties,and the immune response.The streptozotocin(STZ)-induced mouse model exhibits multiple features of human T1D and enables mechanistic analysis of disease progression.However,the relationship between the mechanochemical signaling regulation of STZ-induced T1D and macrophage migration and phagocytosis is unclear.AIM To study the mechanochemical regulation of STZ-induced macrophage response on pancreatic beta islet cells to gain a clearer understanding of T1D.METHODS We performed experiments using different methods.We stimulated isolated pancreatic beta islet cells with STZ and then tested the macrophage migration and phagocytosis.RESULTS In this study,we discovered that the integrin-associated surface factor CD47 played a critical role in immune defense in the STZ-induced T1D model by preventing pancreatic beta islet inflammation.In comparison with healthy mice,STZ-treated mice showed decreased levels of CD47 on islet cells and reduced interaction of CD47 with signal regulatory proteinα(SIRPα),which negatively regulates macrophage-mediated phagocytosis.This resulted in weakened islet cell immune defense and promoted macrophage migration and phagocytosis of target inflammatory cells.Moreover,lipopolysaccharide-activated human acute monocytic leukemia THP-1 cells also exhibited enhanced phagocytosis in the STZ-treated islets,and the aggressive attack of the inflammatory islets correlated with impaired CD47-SIRPαinteractions.In addition,CD47 overexpression rescued the pre-labeled targeted cells.CONCLUSION This study indicates that CD47 deficiency promotes the migration and phagocytosis of macrophages and provides mechanistic insights into T1D by associating the interactions between membrane structures and inflammatory disease progression.

Effect of Sishen pill on expression of type Ⅰ and type Ⅲ interferon in acute ulcerative colitis mice model

Objective:To investigate the effects of Sishen pill on the expression of type I interferon(IFN)and type III interferon and their receptors in colonic tissues of mice with acute ulcerative colitis(UC).Methods:Male C57BL/6Cnc mice were randomly divided into control group,model group,sishenwan group and salazosulfapyridine group.The model was made with 0.2 mL 4%dextran sodium sulfate(DSS)for 5 days,and the control group was given 0.2mL normal saline by gavage.On the second day of modeling,sishen pill group was given 0.2mL 1.5 g·kg^(-1) sishen pill,and SASP group was given 0.2mL 0.25 g·kg^(-1) sulfasalazine,twice a day,for 7 days.During the administration period,the disease activity index(DAI)of mice was calculated every day.After administration,the histopathological changes of colon tissues of mice in each group were observed by hematoxylin eosin(HE)staining,and the histological scores were calculated.The expression of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 mRNAs in colon tissues of mice in each group were detected by qRT-PCR.The expression levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 in colon tissues of mice in each group were detected by ELISA.Western blot was used to detect the expression of interferon receptors IFNAR1,IFNAR2 and IFNLR1 in colon tissues of mice in each group.Results:Compared with the control group,the DAI of mice increased significantly(P<0.001)in the model group.The inflammatory cells in colonic tissues infiltrated heavily,lymph nodes enlarged,colonic mucosal structure destroyed,crypt structure lost,inflammation involved a wide range,and the histological score increased significantly(P<0.001).The levels of IFN-α,IFN-βand IFNλ2 mRNA were significantly decreased(P<0.05,P<0.05,P<0.01).The expression levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 were significantly decreased(P<0.01,P<0.01,P<0.001,P<0.001).The levels of IFNAR1,IFNAR2 and IFNLR1 were significantly decreased(P<0.01,P<0.05,P<0.01).Compared with the model group,the DAI decreased significantly(P<0.001)in Sishen pill group,the infiltration of inflammatory cells in colon tissue were significantly reduced,the structural regeneration of colon mucosa was significantly recovered,the crypt structure was significantly recovered,the lymph nodes were significantly reduced,the range of inflammation involvement was reduced,and the histological score was significantly reduced(P<0.001).The levels of IFN-α,IFN-βand IFN-λ2 mRNA were significantly increased(P<0.01,P<0.001,P<0.001).The levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 were significantly increased(P<0.01,P<0.001,P<0.01,P<0.001).The levels of IFNAR1,IFNAR2 and IFNLR1 were significantly increased(P<0.05,P<0.001,P<0.05).Conclusion:Sishen pill may alleviate the symptoms and signs of mice with acute ulcerative colitis by regulating the expression of type I and type III interferon and their receptors in colon tissues.

基于机场Type Ⅰ ILS设备进行CAT Ⅱ ILS试飞的可行性研究

进行CATⅡILS试飞的前提是机场具备TypeⅡILS设备,国内符合条件的机场皆为大型枢纽机场,诸多运行限制将极大影响试飞效率。为了增加机场选择裕度,加快试飞进程,在国内首次提出采用具有TypeⅠILS进行CATⅡILS试飞的新方法,并从适航条款、技术要求等方面详细分析其可行性。结果表明:此方法满足适航审定要求,切实可行,是一种有效的能实际运用于国内大多数Ⅰ类运行条件机场的试飞方法。

改良尺骨逐步延长法治疗遗传性多发性骨软骨瘤引发的Masada Ⅰ型前臂畸形的疗效

目的探讨改良尺骨逐步延长法治疗由遗传性多发性骨软骨瘤引发的MasadaⅠ型前臂畸形的临床效果。方法收集2014-06至2020-10就诊于首都医科大学附属北京儿童医院骨科,遗传性多发性骨软骨瘤引发的MasadaⅠ型前臂畸形接受改良尺骨逐步延长法手术患者10例(共11肢),男8例,女2例,手术年龄(10.0±2.8)岁,回顾性分析其临床及影像资料,比较手术前后桡骨关节面角度(RAA),腕骨偏移度(CS),相对尺骨短缩长度(RUS)。临床评价腕关节屈曲、伸直活动度,腕关节尺偏、桡偏活动度,前臂旋前、旋后活动度,肘关节屈伸活动范围。结果10例患者术后平均随访(38.2±6.6)个月,尺骨平均延长距离(38.9±6.6)mm。术前与末次随访的RAA[(40.1°±14.2°)vs.(39.3°±13.8°)]、前臂旋后活动度[(48.2°±7.5°)vs.(53.6°±6.0°)]、肘关节屈伸活动度[(135.5°±3.5°)vs.(136.8°±3.4°)]无显著统计学差异(P>0.05);术前与末次随访的CS[(65.0%±22.5%)vs.(34.1%±10.4%)],RUS[(11.2±2.2)mm vs.(-3.9±5.3)mm]、腕关节屈曲活动度[(40.0°±7.7°)vs.(54.5±7.9°)]、腕关节伸直活动度[(45.9°±8.6°)vs.(61.4°±6.4°)],腕关节尺偏活动度[(42.7°±7.5°)vs.(30.5°±6.5°)]、腕关节桡偏活动度[(17.7°±4.7°)vs.(29.1°±4.9°)],前臂旋前活动度[(59.1°±5.4°)vs.(65.0°±3.9°)]存在统计学差异(P<0.05)。随访过程中出现病理性骨折1例。结论改良尺骨逐步延长法能够有效治疗遗传性多发性骨软骨瘤引发的MasadaⅠ型前臂畸形,改善前臂功能。

老年GardenⅠ~Ⅱ型股骨颈骨折病人采用半髋关节置换与内固定治疗的近期价值比较

目的探讨老年GardenⅠ~Ⅱ型股骨颈骨折病人采用半髋关节置换与内固定治疗的近期价值。方法2019年1月~2020年6月我院收治的老年股骨颈骨折病人102例,根据手术治疗方法将其分为对照组51例,观察组51例。对照组予以半髋关节置换术治疗,观察组予以内固定术治疗,比较两组手术相关指标、髋关节功能、髋周肌肉损伤、骨代谢指标及并发症发生情况。结果观察组手术时间、住院时间较对照组短,术中失血量明显较对照组少,观察组术后3及6个月Harris髋关节评分量表(HHS)评分低于对照组,两组比较差异有统计学意义(P<0.05);术后1年及2年,两组HHS评分比较无明显差异。观察组术后3天肌酸激酶(CK)、C反应蛋白(CRP)、白介素(IL)-6)均较同期对照组低,两组比较差异有统计学意义(P<0.05)。观察组术后3个月血清骨钙素蛋白(BGP)、骨碱性磷酸酶(ALP)及骨形态发生蛋白2(BMP-2)均明显较对照组低,两组比较差异有统计学意义(P<0.05);术后1年两组BGP、ALP、BMP-2比较无明显差异。观察组和对照组术后并发症发生率分别为9.80%和15.69%,两组比较差异无统计学意义(P>0.05)。结论老年GardenⅠ~Ⅱ型股骨颈骨折病人内固定术治疗近期效果与半髋关节置换术相当,且前者在手术时间、创伤等方面更具优势。