基于STS-1/AU3时隙交换粒度的SDH/PDH支路线卡的设计与实现

利用PMC-Sierra公司的ASSP芯片PM5332和PM5320,以Freescale的ColdFire处理器MCF5249为硬件控制平台,经过裁减的uClinux操作系统作为软件开发平台,设计并实现了基于STS-1/AU3时隙交换粒度的SDH/PDH支路线卡。该线卡支持三种线侧接口速率,其SDH线侧接口为OC-48与OC-3光接口,而PDH线侧接口为E3/DS3/EC-1电接口。

食管鳞癌中lncRNA uc061hsf.1的表达及对Eca109、KYSE450细胞增殖、凋亡和侵袭的影响

目的:探讨uc061hsf.1基因在食管鳞癌(esophageal squamous cell carcinoma,ESCC)中表达及其对Eca109与KYSE450细胞增殖、凋亡、侵袭能力的影响。方法:本文对34例ESCC组织及癌旁组织进行RT-PCR检测,分析uc061hsf.1的表达与ESCC临床病理特征关系;通过在Eca109与KYSE450细胞系中沉默和上调uc061hsf.1表达,分别检测两组细胞增殖、凋亡、侵袭能力的变化。结果:uc061hsf.1在ESCC组织中低表达,其表达水平在分化差、淋巴结转移的患者中更低(P均<0.05),与年龄、性别等无明显相关性;沉默uc061hsf.1可导致Eca109与KYSE450细胞系增殖及侵袭能力均明显升高,细胞凋亡无明显变化;相反,上调uc061hsf.1在这两组细胞系中的表达,则两组细胞系的增殖和侵袭能力均明显降低,且细胞凋亡率明显增加。结论:uc061hsf.1沉默可促进食管鳞癌Eca109与KYSE450细胞增殖和侵袭能力;而过表达uc061hsf.1能够抑制Eca109与KYSE450细胞增殖和侵袭,且促进细胞凋亡。

Expression of Circulating Plasma Long Non-Coding RNA uc063iod.1 and Its Relationship with Coronary Artery Disease

Objective: To investigate the expression of LncRNA uc063iod.1 in patients with different diseases and to explore the correlation between expression level of LncRNA uc063iod.1 and Coronary Artery Disease (CAD). Method: Plasma samples were prepared using the blood extracted from total 101 patients undergoing coronary artery angiogram (63 CAD patients and 38 non CAD patients). The expression level of LncRNA uc063iod.1 was detected using quantitative real time polymerase chain reaction (qRT-PCR) and the relationship between expression level of LncRNA uc063iod.1 and CAD was explored. The clinicopathological features of patients with CAD were also discussed in our study. Results: The expression of LncRNA uc063iod.1 was significantly increased in the plasma of patients with CAD (p Conclusion: Upregulation of LncRNA uc063iod.1 in plasma can be used as a biomarker for the diagnosis of CAD.

HCV 5'NCR片段调控荧光素酶表达质粒的构建及其在HepG2细胞中的表达

目的构建ＨＣＶ５ＮＣＲ片段调控荧光素酶表达质粒，并在ＨｅｐＧ２细胞中表达。方法通过ＰＣＲ扩增，获得中国人ＨＣＶ基因组５非编码区（ｎｏｎｃｏｄｉｎｇｒｅｇｉｏｎ，ＮＣＲ）完整序列与Ｃ区部分序列的目的基因片段（５ＮＣＲ－Ｃ片段）。将此片段插入ｐＧＬ３荧光素酶报告载体的荧光素酶基因起始密码上游，构建受５ＮＣＲ片段调控的荧光素酶表达质粒。应用脂质体介导基因转染技术将８个质粒转染ＨｅｐＧ２肝癌细胞。结果经鉴定获得８个均为正向插入的阳性克隆。序列分析结果表明插入片段与中国人ＨＣＶ（Ⅱ型）序列基本一致，其荧光素酶基因起始密码子已去除且未改变编码框。有一个质粒能够表达荧光素酶活性，其绝对值达１１４１．９±１５１．１ｍＶ，是ｐＧＬ３报告载体荧光素酶活性的２０％。结论当质粒１μｇ、Ｌｉｐｏｆｅｃｔｉｎ６μｌ。