Ubiquitination,an essential post-transcriptional modification(PTM),plays a vital role in nearly every biological process,including development and growth.Despite its functions in plant reproductive development,its tar...Ubiquitination,an essential post-transcriptional modification(PTM),plays a vital role in nearly every biological process,including development and growth.Despite its functions in plant reproductive development,its targets in rice panicles remain unclear.In this study,we used proteome-wide profiling of lysine ubiquitination in rice(O.sativa ssp.indica)young panicles.We created the largest ubiquitinome dataset in rice to date,identifying 1638 lysine ubiquitination sites on916 unique proteins.We detected three conserved ubiquitination motifs,noting that acidic glutamic acid(E)and aspartic acid(D)were most frequently present around ubiquitinated lysine.Enrichment analysis of Gene Ontology(GO)annotations and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways of these ubiquitinated proteins revealed that ubiquitination plays an important role in fundamental cellular processes in rice young panicles.Interestingly,enrichment analysis of protein domains indicated that ubiquitination was enriched on a variety of receptorlike kinases and cytoplasmic tyrosine and serine-threonine kinases.Furthermore,we analyzed the crosstalk between ubiquitination,acetylation,and succinylation,and constructed a potential protein interaction network within our rice ubiquitinome.Moreover,we identified ubiquitinated proteins related to pollen and grain development,indicating that ubiquitination may play a critical role in the physiological functions in young panicles.Taken together,we reported the most comprehensive lysine ubiquitinome in rice so far,and used it to reveal the functional role of lysine ubiquitination in rice young panicles.展开更多
基金the grants from the National Key Research and Development Program of China(Grant No.2016YFD0100400 to HZ)the National Natural Science Foundation of China(Grant Nos.31501069 to ZL,31601067 to HC,and 31571255 to HZ)+2 种基金the Guangdong Natural Science Funds for Distinguished Young Scholars,China(Grant No.2017A030306001 to HZ)the Pearl River S&T Nova Program of Guangzhou,China(Grant No.201710010106 to HZ)the Scientific Research Project of Guangzhou,China(Grant No.201504010004 to CZ)。
文摘Ubiquitination,an essential post-transcriptional modification(PTM),plays a vital role in nearly every biological process,including development and growth.Despite its functions in plant reproductive development,its targets in rice panicles remain unclear.In this study,we used proteome-wide profiling of lysine ubiquitination in rice(O.sativa ssp.indica)young panicles.We created the largest ubiquitinome dataset in rice to date,identifying 1638 lysine ubiquitination sites on916 unique proteins.We detected three conserved ubiquitination motifs,noting that acidic glutamic acid(E)and aspartic acid(D)were most frequently present around ubiquitinated lysine.Enrichment analysis of Gene Ontology(GO)annotations and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways of these ubiquitinated proteins revealed that ubiquitination plays an important role in fundamental cellular processes in rice young panicles.Interestingly,enrichment analysis of protein domains indicated that ubiquitination was enriched on a variety of receptorlike kinases and cytoplasmic tyrosine and serine-threonine kinases.Furthermore,we analyzed the crosstalk between ubiquitination,acetylation,and succinylation,and constructed a potential protein interaction network within our rice ubiquitinome.Moreover,we identified ubiquitinated proteins related to pollen and grain development,indicating that ubiquitination may play a critical role in the physiological functions in young panicles.Taken together,we reported the most comprehensive lysine ubiquitinome in rice so far,and used it to reveal the functional role of lysine ubiquitination in rice young panicles.
