Optimization of Fermentation Conditions for Cellulase Production from Camellia Seed Shell Using Response Surface Methodology

Objective] This study was conducted to optimize cameI ia seed sheI fer-mentation conditions for ceI uIase production by Trichoderma koningi using response surface methodoIogy. [Method] Fermentation conditions for ceI uIase production from Trichoderma koningi were optimized with response surface method （RSM） by taking carboxymethyI ceI uIase （CMCase） activity as a response indicator. Three factors that affecting CMCase activity were screened out using singIe factor test among pretreatment methods of raw material, nitrogen sources, initial pH values, inocuIum voIume, fermentation time and voIume of Iiquid medium, they were fermentation time, initial pH value, and voIume of Iiquid medium. The optimum conditions of fer-mentation and interaction of the three factors were determined through Box-Behnken design and regression analysis using Design-Expert software. [Result] Pretreatment of cameI ia seed sheI with alkaline was most conducive to CMCase production. The use of 0.2% （NH4）2SO4 as nitrogen source, inocuIum size of 5%, initial pH value of 5.8 and voIume of Iiquid medium at 22 mI were the best fermentation conditions for maximizing CMCase production by T. koningi from cameI ia seed sheI . Under these conditions, 179.15 U/mI of CMCase was obtalned after 5 d of fermentation, which was improved by 24.52% compared with the maximum CMCase activity of singIe factor test. [Conclusion] The resuIts wiI provide some references for use of cameI ia seed sheI and ceI uIase production.

图书馆之城技术平台ULAS研究

ULAS是深圳图书馆在深圳建设“图书馆之城”目标的大环境下，依据原有的dlLAS系统应用经验和设计思路重新设计和创新的产物，是传统图书馆理论升级到Lib3．0、服务核心完全确立下的产物。文章从6个方面将dlLAs和ULAs进行纵向比较，并且针对国内其他图书馆自动化软件，介绍了其主要的功能特点和技术特点，同时介绍了研发中出现的问题及解决方法。