Study of the mechanisms of acupuncture and moxibustion treatmentfor ulcerative colitis ratsinview of the gene expression of cytokines

AIM:To observe the effect of acupuncture and moxibustion on the expression of IL-1beta and IL-6 mRNA in ulcerative colitis rats.METHODS:The SD rat ulcerative colitis model was created by immunological method associated with local stimulation. Colonic mucosa was prepared from human fresh surgical colonic specimens, homogenized by adding appropriate amount of normal saline and centrifuged at 3000r/min. The supernatant was collected for measurement of protein conentration and then mixed with Freund adjuvant. This antigen fluid was first injected into the plantae of the model group rats, and then into their plantae, dorsa, inguina and abdominal cavities (noFreund adjuvant for the last injection) again on the 10th, 17th, 24th and 31st day. When a certain titer of serum anti colonic antibody was reached, 2% formalin and antigen fluid (no Freund adjuvant) were administered separately by enema. The ulcerative colitis rat model was thus set up. The animals were randomly divided into four groups: model control group (MC, n = 8), electro acupuncture group (EA, n = 8), herbs partition moxibustion group (HPM 8), normal control group (NC,n = 8). HPM: Moxa cones made of refined mugwort floss were placed on the medicinal pad (medicinal pad dispensing: Radix Aconiti praeparata, cortex Cinnamomi, etc) for Qihai (RN 6) and Tianshu (ST 25, bilateral) and ignited. Two moxa cones were used for each acupoint once a day and 14 times in all. EA: Tianshu (bilateral) and Qihai were stimulated by the intermittent pulse with 2Hz frequency, 4mA intensity for 20 minutes once a day and 14 times in all. After treatment, rats of all four groups were killed simultaneously. The spleen was separated and the distal colon was dissected. Total tissue RNA was isolated by the guanidinium thiocyanate phenol chloroform extraction method. RT-PCR technique was used to study the expression of IL-1 beta and IL-6 mRNA.RESULTS:IL-1 beta and IL-6 mRNAs were not detected in the spleen and colonic mucosa of the NC rats, whereas they were significantly expressed in that of the MC rats.IL-1 beta and IL-6 mRNAs were markedly lower in the EA and HPM rats than that in MC rats. There was no significant difference between the levels of IL-1 beta and IL-6 mRNAs in the EA and HPM rats. The expressions of IL-1 beta and IL-6 mRNAs were nearly the same in the spleen and colon of all groups.CONCLUSION:Acupuncture and moxibustion greatly inhibited the expression of IL-1 beta and IL-6 mRNA in the experimental ulcerative colitis rats.

23 CASES OF CHRONIC NONSPECIFIC ULCERATIVE COLITIS TREATED BY ACUPUNCTURE AND MOXIBUSTION

In recent years, the author has treated 23 in-patients and out-patients with chronic nonspecific ulcerative colitis and achieved good therapeutic results as reported in the following. Clinical Data In this series, there were 23 cases, 9 males and 14 females, ranging in age from 29 to 68 years, averaging 33.8 years. The duration of disease was 2 to 23 years, with a mean of 11.5 years. All the patients had symptoms and signs of the disease in varying degrees

Auricular-Plaster Therapy Plus Acupuncture at Zusanli for Postoperative Recovery of Intestinal Function

In order to relieve the abdominal distension and other discomforts due to gastrointestinal dysfunction after abdominal operations, the patients were treated by auricular-plaster therapy plus acupuncture at Zusanli (ST 36). 12 (92.4%) of the 13 cases in the treatment group showed recovery of normal peristalsis within 72 hours after operations, while that in 13 cases of the control group was 46.1%, indicating that auricular-plaster therapy plus acupuncture at Zusanli (ST 36) may promote postoperative recovery of the intestinal function.

OBSERVATION OF THE EFFICACY OF ACUPUNCTURE AND MOXIBUSTION IN 62 CASES OF CHRONIC COLITIS

62 patients with chronic colitis were randomly divided into two groups. Acupuncture and moxibustion at acupoints such as Tianshu (St 25), Guanyuan (Ren 4) were applied in the treatment group, and western drugs were applied in the control group. The results showed that acupuncture and moxibustion had a marked curative effective with few side effects, and therefore was readily acceptable to the patients.

A CLINICAL STUDY ON THE SUBTOTAL GASTRECTOMY UNDER ACUPUNCTURE COMBINED WITH EPIDURAL ANESTHESIA OF SMALL DOSAGE

We have performed operations on the patients with perforation of gastric ulcer forsubtotal gastrectomy under acupuncture combined with epidural anesthesia of smaIl dosage （studygroup） or epidural anesthesia only（control gr0up） at rand0m, 2O cases in each group. There is no ob-vious difference of successful rates between tw0 groups （P】O. O5）. But the fluctuation of the bloodpressure of the study group is smaller than that of the control group（PwtO- O1 ）; the comparison of thefluctuations of capacity of vessel and the changes 0f TMwave of ECG before and during operationshows that the study group is better than control gr0up （PrO. O1, P【O. O5, respectively）; in thestudy group, WBC is decreased significantly after operation, while in the control guoup, CRP（C-re-active pr0tein） is markedIy increased, there are significant differences of these indices between twogroups（P【0. 05, respectively）; complement C3, IgA, IgG and IgM are decreased to a different ex- tent after operation in both groups, but the

Effects of electroacupuncture at Chize(LU 5) versus Shangjuxu(ST 37) in rats with ulcerative colitis

Objective： To compare the effects between electroacupuncture （EA） at Chize （LU 5, the He-Sea point of the Lung Meridian） and Shangjuxu （ST 37, the lower He-Sea point of the large intestine） in rats with ulcerative colitis （UC） on the variations of mesenteric microcirculation and vasoactive intestinal peptide （VIP） in the colon, lung, and hypothalamus. The relative specificity of acupoints was also explored. Methods： A total of 28 male Wistar rats were randomized into a normal group, a model group, a Chize （LU 5） group and a Shangjuxu （ST 37） group, 7 rats in each group. The UC model was established by enema with acetic acid. Since the third day after modeling, rats in the Chize （LU 5） group and Shangjuxu （ST 37） group respectively received EA at Chize （LU 5） and Shangjuxu （ST 37）, 1S min each time for successive 7 d. The variations of mesenteric microvascular calibers and blood flow status were observed by a microcirculation microscopic tester; VIP in the colon, lung and hypothalamus was measured by radioimmunoassay. Results： Compared with the normal group, the mesenteric microvascular calibers were significantly expanded in the model group （P〈0.05）; there was no significant difference between the model group and Chize （LU 5） group （P〉0.05）; compared with the model group and Chize （LU 5） group, the calibers were obviously shrunk in Shangjuxu （ST 37） group （P〈0.05）. The four groups showed no significant inter-group differences in comparing blood flow status （P〉0.05）. The colonic VIP levels in the model group and Chize （LU 5） group were significantly higher than that in the normal group （P〈0.01, P〈0.05）; the VIP level in Shangjuxu （ST 37） group was markedly lower than that in the model group （P〈0.01）. There were no significant differences among the four groups in comparing VIP level in lung and hypothalamus （P〉0.05）. Conclusion： The effects of Chize （LU 5） and Shangjuxu （ST 37） were different in treating UC. Shangjuxu （ST 37） showed a more significant efficacy in down-regulating VIP in the colon and regulating mesenteric microcirculation, while the effects of Chize （LU 5） were not obvious.

针刺结合中药保留灌肠对溃疡性结肠炎的疗效及免疫功能的影响

目的:探讨针刺结合中药保留灌肠治疗溃疡性结肠炎(UC)的临床疗效及其改善免疫功能的作用。方法:选取符合纳入标准的UC患者70例,依据随机数字表随机分为观察组和对照组,各35例。对照组给予美沙拉嗪缓释片,观察组在对照组基础上加用针刺与中药保留灌肠疗法,两组均治疗8周。比较两组患者治疗前后血清免疫功能指标CD3^(+)、CD4^(+)与CD8^(+)含量、炎症因子指标白细胞介素(IL)-1β、IL-23与转化生长因子-β1(TGF-β1)的改善情况。结果:治疗后,两组患者TGF-β1、CD3^(+)、CD4^(+)含量均较治疗前明显升高(P<0.05),IL-1β、IL-23、CD8^(+)含量下降(P<0.05);且观察组治疗后TGF-β1、CD3^(+)和CD4^(+)水平均高于对照组(P<0.05),IL-1β、IL-23、CD8^(+)水平均低于对照组(P<0.05)。结论:针刺结合中药保留灌肠治疗能显著改善UC患者免疫功能,降低其炎症因子水平。

针刺联合火龙罐治疗轻中度溃疡性结肠炎的疗效观察

目的观察针刺联合火龙罐治疗轻中度溃疡性结肠炎的临床疗效及对患者血清炎症因子、肠黏膜屏障功能和大便肠道菌群的影响。方法将120例轻中度溃疡性结肠炎患者随机分为观察组和对照组,每组60例。对照组予口服美沙拉嗪片治疗,观察组在对照组治疗基础上予针刺联合火龙罐治疗。观察两组治疗前后溃疡性结肠炎改良梅奥(Mayo)评分、炎症性肠病生存质量问卷(inflammatory bowel disease questionnaire,IBDQ)评分和肠镜Baron量表评分的变化,观察两组治疗前后患者血清炎症因子指标[肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)和C-反应蛋白(C-reactive protein,CRP)]以及肠黏膜屏障功能指标[低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)、二胺氧化酶(diamine oxidase,DAO)和内毒素(endotoxin,ET)]水平的变化,观察两组治疗前后患者大便中肠道菌群(肠球菌、大肠杆菌、乳酸杆菌和双歧杆菌)活菌对数值的变化。比较两组临床疗效。结果观察组总有效率为96.6%,高于对照组的85.7%(P<0.05)。治疗后,两组Mayo和Baron评分均降低(P<0.05),IBDQ评分均升高(P<0.05);且观察组上述评分均优于对照组(P<0.05)。治疗后,两组血清TNF-α、IL-6、CRP、ET、HIF-1α和DAO水平均降低(P<0.05),且观察组均低于对照组(P<0.05)。治疗后,两组肠球菌和大肠杆菌水平均降低(P<0.05),乳酸杆菌和双歧杆菌水平均升高(P<0.05);且观察组上述菌群水平均优于对照组(P<0.05)。结论在口服美沙拉嗪片治疗基础上,针刺联合火龙罐可明显缓解轻中度溃疡性结肠炎患者的临床症状,改善血清炎症因子水平,提高肠黏膜屏障功能,调节大便肠道菌群。

铋剂四联疗法联合针刺督脉背段压痛穴位治疗脾胃虚寒型Hp感染性胃溃疡疗效分析

目的分析铋剂四联疗法联合针刺督脉背段压痛穴位治疗脾胃虚寒型幽门螺杆菌(Hp)感染性胃溃疡的临床疗效。方法选取2020年1月至2021年12月河南科技大学第一附属医院收治的80例脾胃虚寒型Hp感染性胃溃疡患者作为研究对象,按照不同治疗方法将其分为联合组(40例)与对照组(40例),联合组患者采用铋剂四联疗法联合针刺督脉背段压痛穴位治疗,对照组患者单纯采用铋剂四联疗法治疗,对比观察两组患者中医症候评分、溃疡长径、血清胃泌素(GAS)水平、Hp转阴率及临床疗效。结果治疗14 d后,联合组患者胃脘疼痛、食后腹胀、嗳气泛酸、恶心反胃评分以及血清GAS水平均明显低于对照组(t=6.910、6.318、7.140、9.345、8.819,P均<0.001),溃疡长径明显短于对照组(t=18.860,P<0.001);联合组患者Hp转阴率为87.5%,明显高于对照组患者的Hp转阴率67.5%(χ^(2)=4.588,P=0.032);联合组患者中显效26例、有效11例、无效3例,明显优于对照组患者的显效19例、有效10例、无效11例(Z=-1.997,P=0.046)。结论铋剂四联疗法联合针刺督脉背段压痛穴位治疗脾胃虚寒型Hp感染性胃溃疡,可明显降低GAS水平,提高Hp转阴率,改善患者脾胃虚寒症状,促进溃疡愈合。

Survey of Research on Acupuncture Treatment of Peptic Ulcer

In reviewing and analyzing the study on the clinical selection of the acupoints for peptic ulcer in the recent ten years, it was found out that peptic ulcer was mostly treated by combination of multiple acupoints clinically, by the combination of Front-mu point and Back-shu point of the spleen and stomach, and the combination of Front-mu point and Back-shu point of the spleen and stomach with Lower He-sea point, indicating that Front-mu point and Back-shu point of the spleen and stomach and Lower He-sea point have the important ftmctions in the treatment of peptic ulcer.

针灸为主治疗消化性溃疡研究进展

消化性溃疡是临床常见的消化系统疾病,目前多数患者体内均有幽门螺杆菌感染,以铋剂四联[质子泵抑制剂(PPI)+铋剂+2种抗生素]作为主要治疗方式,虽有效果,但仍存在病情容易复发、溃疡面愈合差或者出现耐药性等问题。针灸能有效缓解消化性溃疡的临床症状,促进溃疡愈合,减少复发率,取得较好的临床疗效。文章主要对近5年针灸为主治疗消化性溃疡的研究进行综述。

针刺联合中药治疗溃疡性结肠炎疗效及对相关指标的影响

目的观察针刺联合中药治疗溃疡性结肠炎(UC)近期疗效及对其血红蛋白(Hb)、红细胞比容(Hct水平及肠道菌群和炎症因子的影响。方法选取130例UC患者,按照随机数字表法分为观察组和对照组,每组65例,两组均给予维持水电解质平衡、改善营养等对症治疗,在此基础上对照组给予中药益阳愈溃汤治疗,观察组在对照组基础上联合针刺治疗。观察两组治疗前后中医症候积分、症状消失时间、临床疗效,检测Hb和Hct水平、肠道菌群、肿瘤坏死因子-α(TNF-α)、白介素-33(IL-33)、白介素-10(IL-10)水平。结果治疗后两组中医症候积分显著低于治疗前(P<0.05),且观察组显著低于对照组(P<0.05);观察组发热、腹痛、黏液脓血便、腹泻消失时间显著低于对照组(P<0.05);观察组总有效率为92.3%,显著高于对照组的78.5%(P<0.05);治疗后两组Hb、Hct水平显著高于治疗前(P<0.05),且观察组显著高于对照组(P<0.05);治疗后两组双歧杆菌、乳酸杆菌显著高于治疗前(P<0.05),且观察组显著高于对照组(P<0.05),两组肠球菌与治疗前比较差异无统计学意义(P>0.05);治疗后两组TNF-α、IL-33水平显著低于治疗前(P<0.05),且观察组显著低于对照组(P<0.05),两组IL-10水平显著高于治疗前(P<0.05),且观察组显著高于对照组(P<0.05)。结论针刺联合中药可有效提高UC患者Hb、Hct水平,改善肠道菌群和炎症因子,提高临床疗效。

艾灸对溃疡性结肠炎肠纤维化大鼠结肠成纤维细胞增殖影响的研究

目的从对溃疡性结肠炎大鼠结肠成纤维细胞增殖影响的角度,探讨艾灸防治溃疡性结肠炎肠纤维化的作用机制。方法采用免疫法加局部刺激制备溃疡性结肠炎肠纤维化大鼠模型,随机将大鼠分为正常组、模型组、隔药灸组、温和灸组和西药组。隔药灸组和温和灸组选取天枢、气海穴分别进行隔药灸和温和灸治疗,西药组柳氮磺胺吡啶溶液灌胃治疗,治疗结束后处死大鼠,剖取结肠组织,分离并培养结肠成纤维细胞,流式细胞术观察各组大鼠结肠成纤维细胞增殖情况。结果模型大鼠结肠成纤维细胞增殖率增高(P<0.01),处于G0-G1期细胞减少(P<0.01),G2-M期和S期细胞增多(P<0.01),隔药灸、温和灸疗法能显著改善大鼠结肠成纤维细胞的增殖率(P<0.01),使处于G0-G1期细胞、G2-M期和S期细胞趋向正常。结论UC大鼠CFB增殖加快,隔药灸和温和灸天枢、气海穴治疗能显著抑制大鼠CFB的过度增殖。

不同穴位配伍针刺对消化性溃疡患者血浆内皮素的影响

目的:观察比较中脘、胃俞配伍和公孙、内关配伍针刺治疗消化性溃疡患者血浆内皮素(ET)水平的变化。方法:将50例消化性溃疡患者随机分为中脘、胃俞配伍和公孙、内关配伍2组进行针刺,均采用捻补法及小幅度震颤治疗,观察治疗前后血浆ET水平。结果:中脘、胃俞配伍组治疗后血浆ET水平分别为(78.42±6.74)ng/L、(50.11±3.49)ng/L,治疗前后比较差异有显著性意义(P<0.01)。公孙、内关配伍组治疗前后血浆ET水平分别为(80.13±8.59)ng/L、(48.32±4.73)ng/L,治疗前后比较差异有显著性意义(P<0.01)。2组间治疗后比较,差异光显著性意义(P>0.05)。结论:中脘、胃俞俞募配伍法及公孙、内关配伍针刺治疗消化性溃疡获效可能与ET变化有关,但2种不同配伍方案对疗效和ET变化的影响没有差异。

针刺治疗复发性口腔溃疡疗效观察

目的观察针刺治疗心脾蕴热型复发性口腔溃疡与常规西药治疗的疗效差异。方法将入选的74例患者随机分为两组,针刺组37例,采用针刺疗法,穴取通里、公孙、内庭、合谷等;对照组37例,常规口服维生素B2、维生素C片治疗,观察并对比两组疗效。结果针刺组总有效率为83·8%,对照组为48·6%,两组疗效差异有非常显著性意义(P<0·01)。结论针刺组疗效明显优于对照组,针刺是治疗心脾蕴热型复发性口腔溃疡的有效方法。

电针耳胃点对溃疡病患者胃电图的影响

通过电针刺激耳胃点、耳心点及静卧对６０例胃溃疡和十二指肠球部溃疡患者胃电图影响的比较观察，证实电针刺激耳胃点能明显地降低溃疡病患者胃窦和胃体部胃电幅值（Ｐ＜０．０１），而电针刺激耳心点和静卧则无此作用（Ｐ＞０．０５）；３种方法对胃窦和胃体部胃电频率影响不大（Ｐ＞０．０５）。提示电针耳胃点对溃疡病有一定的治疗作用。

瑞巴派特联合三联疗法在幽门螺杆菌阳性胃十二指肠溃疡急性穿孔保守治疗中的应用研究

目的:探讨瑞巴派特联合三联疗法在幽门螺杆菌(Helicobacter pylori,Hp)阳性胃十二指肠溃疡急性穿孔保守治疗中的应用。方法:选取2015年10月至2019年12月德阳市人民医院收治住院的Hp阳性胃十二指肠溃疡急性穿孔患者105例,采用随机数字表法将患者分为研究组(n=55)和对照组(n=50)。两组患者均进行保守治疗,对照组患者在此基础上采用三联疗法,研究组患者在对照组的基础上给予瑞巴派特治疗。观察两组患者的临床疗效、住院情况、Hp根除情况及溃疡愈合情况,治疗前后血清炎症因子[肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)]和胃肠激素[胃泌素(Gas)、胃蛋白酶原Ⅰ(PGⅠ)和胃蛋白酶原Ⅱ(PGⅡ)]水平,记录并比较研究期间两组患者不良反应发生情况的差异。结果:研究组患者的总有效率为94.55%(52/55),明显高于对照组的82.00%(41/50),差异有统计学意义(P<0.05)。研究组患者临床症状缓解时间、胃肠减压时间和住院时间明显短于对照组,差异均有统计学意义(P<0.05)。研究组患者的Hp根除率为90.91%(50/55),明显高于对照组的76.00%(38/50);研究组患者溃疡愈合率为87.27%(48/55),明显高于对照组的68.00%(34/50),差异均有统计学意义(P<0.05)。治疗后,两组患者血清TNF-α和IL-6水平明显低于治疗前,且研究组患者明显低于对照组;治疗后,两组患者Gas、PGⅠ和PGⅡ水平明显高于治疗前,且研究组患者明显高于对照组,差异均有统计学意义(P<0.05)。研究组、对照组患者不良反应发生率分别为9.09%(5/55)、12.00%(6/50),差异无统计学意义(P>0.05)。结论:瑞巴派特联合三联疗法可有效减轻进行保守治疗的Hp阳性胃十二指肠溃疡急性穿孔患者的炎症反应,改善胃肠功能,促进溃疡面愈合,提高Hp根除率,缩短临床症状缓解时间、胃肠减压时间和住院时间,疗效确切,具有一定的临床应用价值。

针灸十字穴为主治疗溃疡性结肠炎疗效观察

目的观察针灸十字穴为主治疗溃疡性结肠炎的临床疗效及对免疫的影响。方法将63例溃疡性结肠炎患者随机分为治疗组33例和对照组30例。治疗组采用针灸治疗,对照组采用柳氮磺吡啶配合甲硝唑治疗。观察两组患者临床疗效及治疗前后IgG、IgM、IgA水平变化。结果治疗组总有效率为90.9%,对照组为73.3%。两组比较差异具有统计学意义(P<0.05)。两组治疗后IgG指标与同组治疗前比较,差异均具有统计学意义(P<0.01)。治疗组治疗后IgG指标与对照组比较,差异具有统计学意义(P<0.01)。结论针灸十字穴是一种治疗溃疡性结肠炎的有效方法。

胃溃疡穿孔合并中毒性肾小管坏死的探讨

目的探讨胃溃疡穿孔合并中毒性肾小管坏死的诊断方法与治疗方案。方法报道一例胃溃疡穿孔合并中毒性肾小管坏死的病例(包括尸检)、进行了总结。结果胃溃疡穿孔合并中毒性胃小管坏死可合并肾小管损伤坏死,发展为急性肾衰竭。结论病史,仔细查体,实验检查、影像学检查和病程对于诊断与治疗是非常重要的。

'97针灸治疗溃疡性结肠炎研究进展

从针刺疗法、艾灸疗法和穴位埋线、穴位敷贴等其他疗法角度对’９７国内有关针灸治疗溃疡性结肠炎的概况进行概括和总结，并介绍了该病实验研究方面的进展情况，认为针灸是治疗溃疡性结肠炎的一种有效疗法，且应从细胞因子及其基因表达、一氧化氮及一氧化氮合酶ｍＲＮＡ表达的调控等方面进一步深入研究。