Curcumin,a safe natural yellow pigment with a wide range of pharmacological activities,is used both in herbal drugs and as a food coloring agents.Studies have shown that curcumin would suffer from extensive metabolism...Curcumin,a safe natural yellow pigment with a wide range of pharmacological activities,is used both in herbal drugs and as a food coloring agents.Studies have shown that curcumin would suffer from extensive metabolism in vivoand the predominant metabolic pathways are reduction and conjugation.In order to comprehensively study the metabolism and enrich the metabolic profile of cxurcumin in vivo,we carried out this research.A systematic method with highly sensitive UPLC-Q/TOF-MS was established to analyze different biological samples of rats after展开更多
建立了QuEChERS净化结合超高效液相色谱串联四级杆飞行时间质谱(UHPLC-Q-TOF/MS)法同时筛查猪肉中10种抗生素类兽药残留量的分析方法。样品经过Na;EDTA-Mcllvaine缓冲溶液-0.1%甲酸乙腈-NaCl-无水Na_(2)SO_(4)-C_(18)的提取净化。通过In...建立了QuEChERS净化结合超高效液相色谱串联四级杆飞行时间质谱(UHPLC-Q-TOF/MS)法同时筛查猪肉中10种抗生素类兽药残留量的分析方法。样品经过Na;EDTA-Mcllvaine缓冲溶液-0.1%甲酸乙腈-NaCl-无水Na_(2)SO_(4)-C_(18)的提取净化。通过Infinity Lab Poroshell 120 EC-C_(18)色谱柱分离,在正离子多反应监测模式下分析。结果表明,10种兽药相关系数大于0.99,在5.0~100.0μg/kg范围内线性关系良好,在5、10、20μg/kg三个添加水平下平均回收率为72.3%~117.3%,日内相对标准偏差为(Relative Standard Deviation,RSD)0.9%~10.4%,日间RSD为1.1%~13.4%,检测限(Limit of Detection,LOD)为0.25~1.0μg/kg,定量限(Limit of Quantity,LOQ)为0.5~5.0μg/kg。采用本方法对市场购买的50份新鲜生猪肉样品进行检测,1份样品四环素呈阳性,其余样品均未检出。该方法操作简便、快速,具有良好的精密度和定量限,适用于猪肉中10种抗生素类兽药的快速检测。展开更多
建立一种快速测定水产中地西泮的方法。5%氨水乙腈提取,通过EMR固相萃取柱净化,旋蒸浓缩并定容,用内标法对其进行定量。通过超高效液相色谱串联质谱仪测定,使用Agilent ZORBAX Eclipse Plus RRHD C 18柱子分离,流动相乙腈:0.1%甲酸水,...建立一种快速测定水产中地西泮的方法。5%氨水乙腈提取,通过EMR固相萃取柱净化,旋蒸浓缩并定容,用内标法对其进行定量。通过超高效液相色谱串联质谱仪测定,使用Agilent ZORBAX Eclipse Plus RRHD C 18柱子分离,流动相乙腈:0.1%甲酸水,内标法定量,该方法测得的地西泮检出限0.33μg·kg^(-1)和定量限1.1μg·kg^(-1),对样品进行1.10,3.30,11.0μg·kg^(-1)三个梯度加标,平均回收率85.6%~103.3%,精密度为0.8%~3.5%。该研究方法提取简单,回收率和精密度满足要求,可为水产中地西泮残留的检测标准制定提供依据。展开更多
基金partly supported by National Natural Science Foundation of China(No.81430095)also by Special National Program on Key Basic Research Project(No.2014CB560706)
文摘Curcumin,a safe natural yellow pigment with a wide range of pharmacological activities,is used both in herbal drugs and as a food coloring agents.Studies have shown that curcumin would suffer from extensive metabolism in vivoand the predominant metabolic pathways are reduction and conjugation.In order to comprehensively study the metabolism and enrich the metabolic profile of cxurcumin in vivo,we carried out this research.A systematic method with highly sensitive UPLC-Q/TOF-MS was established to analyze different biological samples of rats after
文摘建立了QuEChERS净化结合超高效液相色谱串联四级杆飞行时间质谱(UHPLC-Q-TOF/MS)法同时筛查猪肉中10种抗生素类兽药残留量的分析方法。样品经过Na;EDTA-Mcllvaine缓冲溶液-0.1%甲酸乙腈-NaCl-无水Na_(2)SO_(4)-C_(18)的提取净化。通过Infinity Lab Poroshell 120 EC-C_(18)色谱柱分离,在正离子多反应监测模式下分析。结果表明,10种兽药相关系数大于0.99,在5.0~100.0μg/kg范围内线性关系良好,在5、10、20μg/kg三个添加水平下平均回收率为72.3%~117.3%,日内相对标准偏差为(Relative Standard Deviation,RSD)0.9%~10.4%,日间RSD为1.1%~13.4%,检测限(Limit of Detection,LOD)为0.25~1.0μg/kg,定量限(Limit of Quantity,LOQ)为0.5~5.0μg/kg。采用本方法对市场购买的50份新鲜生猪肉样品进行检测,1份样品四环素呈阳性,其余样品均未检出。该方法操作简便、快速,具有良好的精密度和定量限,适用于猪肉中10种抗生素类兽药的快速检测。
文摘建立一种快速测定水产中地西泮的方法。5%氨水乙腈提取,通过EMR固相萃取柱净化,旋蒸浓缩并定容,用内标法对其进行定量。通过超高效液相色谱串联质谱仪测定,使用Agilent ZORBAX Eclipse Plus RRHD C 18柱子分离,流动相乙腈:0.1%甲酸水,内标法定量,该方法测得的地西泮检出限0.33μg·kg^(-1)和定量限1.1μg·kg^(-1),对样品进行1.10,3.30,11.0μg·kg^(-1)三个梯度加标,平均回收率85.6%~103.3%,精密度为0.8%~3.5%。该研究方法提取简单,回收率和精密度满足要求,可为水产中地西泮残留的检测标准制定提供依据。
