AIM:To evaluate whether contrast enhanced ultrasound(CEUS) might also be used for response prediction and early response evaluation in patients receiving bevacizumab based chemotherapy for metastasized colorectal canc...AIM:To evaluate whether contrast enhanced ultrasound(CEUS) might also be used for response prediction and early response evaluation in patients receiving bevacizumab based chemotherapy for metastasized colorectal cancer.METHODS:Thirty consecutive patients with non primary resectable liver metastases from colorectal cancer underwent CEUS before treatment(CEUS date 1) and before the second(CEUS date 2) and fourth(CEUS date 3) cycle of bevacizumab based chemotherapy.Three parameters [PEAK,Time to peak(TTP) and RISE RATE]were correlated with radiological response.RESULTS:For neoadjuvant purpose a reduction of tumour mass was required to assume clinical response.Based on these response criteria there was a significant(P < 0.001) correlation in TTP between metastases of responders(9.08 s) and non-responders(14.76 s) archived on CEUS date 1.By calculating a standardized quotient(metastases divided by normal liver tissue) we were able to define a cut off,predicting response with a sensitivity of 92.3 % and a specificity of 100 %.To reflect a palliative intention only those patients with progressive disease were classified as non-responders.In this stetting TTP was also significantly(P < 0.01) different between responders and non-responders.In contrast,Peak and Rise rate did not show any significant difference between responder and non-responder.CONCLUSION:CEUS might serve as a surrogate marker to predict treatment response in patients with metastasized colorectal cancer who receive antiangiogenic therapy.展开更多
Objective: To reach the recovery and identification of antioxidant polyphenolic compounds from Nephelium lappaceum L.(Mexican variety) husk using ultrasound-assisted extraction and liquid chromatography/mass spectrome...Objective: To reach the recovery and identification of antioxidant polyphenolic compounds from Nephelium lappaceum L.(Mexican variety) husk using ultrasound-assisted extraction and liquid chromatography/mass spectrometry as well as the in vitro antioxidant activity. Methods: Rambutan husk extracts were obtained by ultrasound-assisted extraction, mass/volume ratio, water/ethanol percentage and extraction time were evaluated. Once the best extraction condition of polyphenolic compounds was defined, a polyphenolic fraction was recovered using Ambetlite XAD-16. The total content of antioxidant polyphenolic compounds was determined by summation of the total hydrolysable polyphenol and total condensed polyphenol contents. Recovered compounds were identified by FTIR(ATR) spectroscopy and HPLC/ESI/MS. The antioxidant activity was carried out by ABTS, DPPH and lipid oxidation inhibition in vitro methods. Results: In Mexican variety rambutan husk, the total polyphenolic content was 487.67 mg/g, after ultrasound-assisted extraction. According to the HPLC/ESI/MS analysis 12 antioxidant polyphenolic compounds were identified, mostly ellagitannins such as geraniin, corilagin and ellagic acid. The antioxidant activity determined by ABTS, DPPH and lipid oxidation inhibition methods was demonstrated. The main functional groups of the identified compounds were determined by FTIR analysis. Conclusions: It was demonstrated that ultrasound-assisted extraction was effective and allowed the extraction and recovery of antioxidant polyphenolic compounds. Furthermore Mexican variety rambutan husk is an important source for recovering polyphenolic compounds with antioxidant activity, these compounds have potential application for the treatment/prevention of various diseases related to cancer and pathogenic microorganisms.展开更多
OBJECTIVE The aim of the study was to examine the reversal effects of ultrasound(US) on the MDR in HepG2/ADM,a HepG2 cell line resistant to Adriamycin(ADM),and to study the mechanism of US action.METHODS Using the MTT...OBJECTIVE The aim of the study was to examine the reversal effects of ultrasound(US) on the MDR in HepG2/ADM,a HepG2 cell line resistant to Adriamycin(ADM),and to study the mechanism of US action.METHODS Using the MTT assay,the effects of US on MDR in HepG2/ADR cells were studied.Before and after the treatment with 0.5W/cm2 low intensity ultrasound(LIUS),the expression of the MDR-related genes,mdr1,mrp and lrp was assayed with the reverse transcriptase polymerase chain reaction(RT-PCR)and the levels of their respective protein expression determined by flow cytometry.By using confocal laser scanning microscopy(CLSM),we examined the intracellular daunorubicin (DNR) distribution,and the effects on the cells of treatment with US or DNR. RESULTS LIUS significantly reversed MDR in HepG2/ADR cells.A er treatment with LIUS at 0.5W/cm2,chemosensitivity to ADM and DNR increased 3.35-fold and 2.81-fold,respectively.The reversal activity by LIUS plus verapamil(VER) was stronger than with either US or VER alone.A er treatment with 0.5W/cm2 ,the expression of both the MDR1 and the MRP mRNA genes began to decline(P<0.01 and P<0.05,respectively);the expression of LRP showed no significant changes.Changes in the expression of the P-glycoprotein(P-gp) and MRP were similar to those of their mRNA expressions.Results of the CLSM showed that administration of US(0.5W/cm2) or VER(15.7μM) with DNR to HepG2/ADM cells showed a significant change in the distribution of DNR in the cells. CONCLUSION Our results show that LIUS can reverse MDR. The reversal effects are stronger than those of either US or VER alone,when combined with VER administration.As LIUS is non-invasive causing no toxicity,it might have potential for clinical application.The reversal mechanism needs further study.展开更多
Purpose:To compare the measurement of flap thickness using intraoperative ultrasound pachymetry and postoperative visante anterior segment optical coherence tomography (Visante-OCT) in eyes receiving laser in situ ker...Purpose:To compare the measurement of flap thickness using intraoperative ultrasound pachymetry and postoperative visante anterior segment optical coherence tomography (Visante-OCT) in eyes receiving laser in situ keratomileusis (LASIK). Methods: Seventeen patients (34 eyes) received LASIK using a Technolas-217Z100 laser and AMADEUSⅡ mechanical microkeratome(140 μm head). Flap thickness was assessed with an ultrasound pachymeter intraoperatively and a Visante-OCT postoperatively at 1 week and 1 month. Results: The intraoperative mean flap thickness by ultrasound pachymetry was (124±13.9) μm (range: 96.5 to 160 μm), and mean flap thickness by Visante-OCT was (145±7.13) μm (range: 130 to 158 μm) and (143±5.32) μm (range: 132 to 155 μm) postoperatively at 1 week and 1 month respectively (F=63.52,P<0.01). Intraoperative subtraction pachymetry underestimated flap thickness compared with postoperative Visante-OCT.(P<0.01)..There was no significant difference between postoperative Visante-OCT measurements at 1 week and 1 month after LASIK.(P=0.16). The 95% limit of agreement.(LoA).of flap thickness between ultrasound Visante-OCT and pachymetry was -5.40 to 42.10 μm. Conclusion: OCT overestimates flap thickness compared with intraoperative ultrasound pachymetry when using the AMADEUSⅡ mechanical microkeratome.展开更多
文摘AIM:To evaluate whether contrast enhanced ultrasound(CEUS) might also be used for response prediction and early response evaluation in patients receiving bevacizumab based chemotherapy for metastasized colorectal cancer.METHODS:Thirty consecutive patients with non primary resectable liver metastases from colorectal cancer underwent CEUS before treatment(CEUS date 1) and before the second(CEUS date 2) and fourth(CEUS date 3) cycle of bevacizumab based chemotherapy.Three parameters [PEAK,Time to peak(TTP) and RISE RATE]were correlated with radiological response.RESULTS:For neoadjuvant purpose a reduction of tumour mass was required to assume clinical response.Based on these response criteria there was a significant(P < 0.001) correlation in TTP between metastases of responders(9.08 s) and non-responders(14.76 s) archived on CEUS date 1.By calculating a standardized quotient(metastases divided by normal liver tissue) we were able to define a cut off,predicting response with a sensitivity of 92.3 % and a specificity of 100 %.To reflect a palliative intention only those patients with progressive disease were classified as non-responders.In this stetting TTP was also significantly(P < 0.01) different between responders and non-responders.In contrast,Peak and Rise rate did not show any significant difference between responder and non-responder.CONCLUSION:CEUS might serve as a surrogate marker to predict treatment response in patients with metastasized colorectal cancer who receive antiangiogenic therapy.
文摘Objective: To reach the recovery and identification of antioxidant polyphenolic compounds from Nephelium lappaceum L.(Mexican variety) husk using ultrasound-assisted extraction and liquid chromatography/mass spectrometry as well as the in vitro antioxidant activity. Methods: Rambutan husk extracts were obtained by ultrasound-assisted extraction, mass/volume ratio, water/ethanol percentage and extraction time were evaluated. Once the best extraction condition of polyphenolic compounds was defined, a polyphenolic fraction was recovered using Ambetlite XAD-16. The total content of antioxidant polyphenolic compounds was determined by summation of the total hydrolysable polyphenol and total condensed polyphenol contents. Recovered compounds were identified by FTIR(ATR) spectroscopy and HPLC/ESI/MS. The antioxidant activity was carried out by ABTS, DPPH and lipid oxidation inhibition in vitro methods. Results: In Mexican variety rambutan husk, the total polyphenolic content was 487.67 mg/g, after ultrasound-assisted extraction. According to the HPLC/ESI/MS analysis 12 antioxidant polyphenolic compounds were identified, mostly ellagitannins such as geraniin, corilagin and ellagic acid. The antioxidant activity determined by ABTS, DPPH and lipid oxidation inhibition methods was demonstrated. The main functional groups of the identified compounds were determined by FTIR analysis. Conclusions: It was demonstrated that ultrasound-assisted extraction was effective and allowed the extraction and recovery of antioxidant polyphenolic compounds. Furthermore Mexican variety rambutan husk is an important source for recovering polyphenolic compounds with antioxidant activity, these compounds have potential application for the treatment/prevention of various diseases related to cancer and pathogenic microorganisms.
基金a grant from National Natural Science Foundation of China (No.30200060)
文摘OBJECTIVE The aim of the study was to examine the reversal effects of ultrasound(US) on the MDR in HepG2/ADM,a HepG2 cell line resistant to Adriamycin(ADM),and to study the mechanism of US action.METHODS Using the MTT assay,the effects of US on MDR in HepG2/ADR cells were studied.Before and after the treatment with 0.5W/cm2 low intensity ultrasound(LIUS),the expression of the MDR-related genes,mdr1,mrp and lrp was assayed with the reverse transcriptase polymerase chain reaction(RT-PCR)and the levels of their respective protein expression determined by flow cytometry.By using confocal laser scanning microscopy(CLSM),we examined the intracellular daunorubicin (DNR) distribution,and the effects on the cells of treatment with US or DNR. RESULTS LIUS significantly reversed MDR in HepG2/ADR cells.A er treatment with LIUS at 0.5W/cm2,chemosensitivity to ADM and DNR increased 3.35-fold and 2.81-fold,respectively.The reversal activity by LIUS plus verapamil(VER) was stronger than with either US or VER alone.A er treatment with 0.5W/cm2 ,the expression of both the MDR1 and the MRP mRNA genes began to decline(P<0.01 and P<0.05,respectively);the expression of LRP showed no significant changes.Changes in the expression of the P-glycoprotein(P-gp) and MRP were similar to those of their mRNA expressions.Results of the CLSM showed that administration of US(0.5W/cm2) or VER(15.7μM) with DNR to HepG2/ADM cells showed a significant change in the distribution of DNR in the cells. CONCLUSION Our results show that LIUS can reverse MDR. The reversal effects are stronger than those of either US or VER alone,when combined with VER administration.As LIUS is non-invasive causing no toxicity,it might have potential for clinical application.The reversal mechanism needs further study.
文摘Purpose:To compare the measurement of flap thickness using intraoperative ultrasound pachymetry and postoperative visante anterior segment optical coherence tomography (Visante-OCT) in eyes receiving laser in situ keratomileusis (LASIK). Methods: Seventeen patients (34 eyes) received LASIK using a Technolas-217Z100 laser and AMADEUSⅡ mechanical microkeratome(140 μm head). Flap thickness was assessed with an ultrasound pachymeter intraoperatively and a Visante-OCT postoperatively at 1 week and 1 month. Results: The intraoperative mean flap thickness by ultrasound pachymetry was (124±13.9) μm (range: 96.5 to 160 μm), and mean flap thickness by Visante-OCT was (145±7.13) μm (range: 130 to 158 μm) and (143±5.32) μm (range: 132 to 155 μm) postoperatively at 1 week and 1 month respectively (F=63.52,P<0.01). Intraoperative subtraction pachymetry underestimated flap thickness compared with postoperative Visante-OCT.(P<0.01)..There was no significant difference between postoperative Visante-OCT measurements at 1 week and 1 month after LASIK.(P=0.16). The 95% limit of agreement.(LoA).of flap thickness between ultrasound Visante-OCT and pachymetry was -5.40 to 42.10 μm. Conclusion: OCT overestimates flap thickness compared with intraoperative ultrasound pachymetry when using the AMADEUSⅡ mechanical microkeratome.
