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A review of reservoir damage during hydraulic fracturing of deep and ultra-deep reservoirs 被引量:2
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作者 Kun Zhang Xiong-Fei Liu +6 位作者 Dao-Bing Wang Bo Zheng Tun-Hao Chen Qing Wang Hao Bai Er-Dong Yao Fu-Jian Zhou 《Petroleum Science》 SCIE EI CAS CSCD 2024年第1期384-409,共26页
Deep and ultra-deep reservoirs have gradually become the primary focus of hydrocarbon exploration as a result of a series of significant discoveries in deep hydrocarbon exploration worldwide.These reservoirs present u... Deep and ultra-deep reservoirs have gradually become the primary focus of hydrocarbon exploration as a result of a series of significant discoveries in deep hydrocarbon exploration worldwide.These reservoirs present unique challenges due to their deep burial depth(4500-8882 m),low matrix permeability,complex crustal stress conditions,high temperature and pressure(HTHP,150-200℃,105-155 MPa),coupled with high salinity of formation water.Consequently,the costs associated with their exploitation and development are exceptionally high.In deep and ultra-deep reservoirs,hydraulic fracturing is commonly used to achieve high and stable production.During hydraulic fracturing,a substantial volume of fluid is injected into the reservoir.However,statistical analysis reveals that the flowback rate is typically less than 30%,leaving the majority of the fluid trapped within the reservoir.Therefore,hydraulic fracturing in deep reservoirs not only enhances the reservoir permeability by creating artificial fractures but also damages reservoirs due to the fracturing fluids involved.The challenging“three-high”environment of a deep reservoir,characterized by high temperature,high pressure,and high salinity,exacerbates conventional forms of damage,including water sensitivity,retention of fracturing fluids,rock creep,and proppant breakage.In addition,specific damage mechanisms come into play,such as fracturing fluid decomposition at elevated temperatures and proppant diagenetic reactions at HTHP conditions.Presently,the foremost concern in deep oil and gas development lies in effectively assessing the damage inflicted on these reservoirs by hydraulic fracturing,comprehending the underlying mechanisms,and selecting appropriate solutions.It's noteworthy that the majority of existing studies on reservoir damage primarily focus on conventional reservoirs,with limited attention given to deep reservoirs and a lack of systematic summaries.In light of this,our approach entails initially summarizing the current knowledge pertaining to the types of fracturing fluids employed in deep and ultra-deep reservoirs.Subsequently,we delve into a systematic examination of the damage processes and mechanisms caused by fracturing fluids within the context of hydraulic fracturing in deep reservoirs,taking into account the unique reservoir characteristics of high temperature,high pressure,and high in-situ stress.In addition,we provide an overview of research progress related to high-temperature deep reservoir fracturing fluid and the damage of aqueous fracturing fluids to rock matrix,both artificial and natural fractures,and sand-packed fractures.We conclude by offering a summary of current research advancements and future directions,which hold significant potential for facilitating the efficient development of deep oil and gas reservoirs while effectively mitigating reservoir damage. 展开更多
关键词 Artificial fracture Deep and ultra-deep reservoir Fracture conductivity Fracturing fluid Hydraulic fracturing Reservoir damage
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Geochemical characteristics and exploration significance of ultra-deep Sinian oil and gas from Well Tashen 5,Tarim Basin,NW China 被引量:2
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作者 Zicheng Cao Anlai Ma +4 位作者 Qinqi Xu Quanyong Pan Kai Shang Fan Feng Yongli Liu 《Energy Geoscience》 EI 2024年第1期24-36,共13页
The Well Tashen 5(TS5),drilled and completed at a vertical depth of 9017 m in the Tabei Uplift of the Tarim Basin,NW China,is the deepest well in Asia.It has been producing both oil and gas from the Sinian at a depth ... The Well Tashen 5(TS5),drilled and completed at a vertical depth of 9017 m in the Tabei Uplift of the Tarim Basin,NW China,is the deepest well in Asia.It has been producing both oil and gas from the Sinian at a depth of 8780e8840 m,also the deepest in Asia in terms of oil discovery.In this paper,the geochemical characteristics of Sinian oil and gas from the well were investigated and compared with those of Cambrian oil and gas discovered in the same basin.The oil samples,with Pr/Ph ratio of 0.78 and a whole oil carbon isotopic value of31.6‰,have geochemical characteristics similar to those of Ordovician oils from the No.1 fault in the North Shuntuoguole area(also named Shunbei area)and the Middle Cambrian oil from wells Zhongshen 1(ZS1)and Zhongshen 5(ZS5)of Tazhong Uplift.The maturity of light hydrocarbons,diamondoids and aromatic fractions all suggest an approximate maturity of 1.5%e1.7%Ro for the samples.The(4-+3-)methyldiamantane concentration of the samples is 113.5 mg/g,indicating intense cracking with a cracking degree of about 80%,which is consistent with the high bottom hole temperature(179℃).The Sinian gas samples are dry with a dryness coefficient of 0.97.The gas is a mixture of kerogen-cracking gas and oil-cracking gas and has Ro values ranging between 1.5%and 1.7%,and methane carbon isotopic values of41.6‰.Based on the equivalent vitrinite reflectance(R_(eqv)=1.51%e1.61%)and the thermal evolution of source rocks from the Cambrian Yu'ertusi Formation of the same well,it is proposed that the Sinian oil and gas be mainly sourced from the Cambrian Yu'ertusi Formation during the Himalayan period but probably also be joined by hydrocarbon of higher maturity that migrated from other source rocks in deeper formations.The discovery of Sinian oil and gas from Well TS5 suggests that the ancient ultra-deep strata in the northern Tarim Basin have the potential for finding volatile oil or condensate reservoirs. 展开更多
关键词 ultra-deep Sinian oil and gas Oil-cracking Well Tashen 5 Tarim Basin
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Multi-lump Kinetic Parameter Estimation and Simulation of Trickle-bed Reactor for Ultra-deep Hydrodesulfurization of Diesel
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作者 Huang Zhen Zhang Xun +4 位作者 Qiao Aijun Xue Nan Liu Kaixiang Xu Song Wang He 《China Petroleum Processing & Petrochemical Technology》 SCIE CAS CSCD 2024年第3期147-157,共11页
A three-lumping Langmuir-Hinshelwood kinetic model was established based on the structures and reactivities of sulfur compounds.This model described the ultra-deep hydrodesulfurization(UDHDS)performance of diesel,redu... A three-lumping Langmuir-Hinshelwood kinetic model was established based on the structures and reactivities of sulfur compounds.This model described the ultra-deep hydrodesulfurization(UDHDS)performance of diesel,reducing sulfur content from 10000μg/g to less than 10μg/g,with experimental and predicted data showing a discrepancy of less than 10%.The diesel UDHDS reaction was simulated by combining the mass transfer,reaction kinetics model,and physical properties of diesel.The results showed how the concentrations of H2S,hydrogen,and sulfur in the gas,liquid,and solid phases varied along the reactor length.Moreover,the study discussed the effects of each process parameter and impurity concentrations(H2S,basic nitrogen and,non-basic nitrogen)on diesel UDHDS. 展开更多
关键词 DIESEL TBR ultra-deep HDS kinetic model simulation
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Supramolecular polymer-based gel fracturing fluid with a double network applied in ultra-deep hydraulic fracturing
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作者 Yong-Ping Huang Yong Hu +5 位作者 Chang-Long Liu Yi-Ning Wu Chen-Wei Zou Li-Yuan Zhang Ming-Wei Zhao Cai-Li Dai 《Petroleum Science》 SCIE EI CAS CSCD 2024年第3期1875-1888,共14页
A gel based on polyacrylamide,exhibiting delayed crosslinking characteristics,emerges as the preferred solution for mitigating degradation under conditions of high temperature and extended shear in ultralong wellbores... A gel based on polyacrylamide,exhibiting delayed crosslinking characteristics,emerges as the preferred solution for mitigating degradation under conditions of high temperature and extended shear in ultralong wellbores.High viscosity/viscoelasticity of the fracturing fluid was required to maintain excellent proppant suspension properties before gelling.Taking into account both the cost and the potential damage to reservoirs,polymers with lower concentrations and molecular weights are generally preferred.In this work,the supramolecular action was integrated into the polymer,resulting in significant increases in the viscosity and viscoelasticity of the synthesized supramolecular polymer system.The double network gel,which is formed by the combination of the supramolecular polymer system and a small quantity of Zr-crosslinker,effectively resists temperature while minimizing permeability damage to the reservoir.The results indicate that the supramolecular polymer system with a molecular weight of(268—380)×10^(4)g/mol can achieve the same viscosity and viscoelasticity at 0.4 wt%due to the supramolecular interaction between polymers,compared to the 0.6 wt%traditional polymer(hydrolyzed polyacrylamide,molecular weight of 1078×10^(4)g/mol).The supramolecular polymer system possessed excellent proppant suspension properties with a 0.55 cm/min sedimentation rate at 0.4 wt%,whereas the0.6 wt%traditional polymer had a rate of 0.57 cm/min.In comparison to the traditional gel with a Zrcrosslinker concentration of 0.6 wt%and an elastic modulus of 7.77 Pa,the double network gel with a higher elastic modulus(9.00 Pa)could be formed only at 0.1 wt%Zr-crosslinker,which greatly reduced the amount of residue of the fluid after gel-breaking.The viscosity of the double network gel was66 m Pa s after 2 h shearing,whereas the traditional gel only reached 27 m Pa s. 展开更多
关键词 ultra-deep reservoir Gel fracturing fluid Double network Supramolecular polymer system Proppant suspension property
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Research advances on the mechanisms of reservoir formation and hydrocarbon accumulation and the oil and gas development methods of deep and ultra-deep marine carbonates
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作者 MA Yongsheng CAI Xunyu +9 位作者 LI Maowen LI Huili ZHU Dongya QIU Nansheng PANG Xiongqi ZENG Daqian KANG Zhijiang MA Anlai SHI Kaibo ZHANG Juntao 《Petroleum Exploration and Development》 SCIE 2024年第4期795-812,共18页
Based on the new data of drilling, seismic, logging, test and experiments, the key scientific problems in reservoir formation, hydrocarbon accumulation and efficient oil and gas development methods of deep and ultra-d... Based on the new data of drilling, seismic, logging, test and experiments, the key scientific problems in reservoir formation, hydrocarbon accumulation and efficient oil and gas development methods of deep and ultra-deep marine carbonate strata in the central and western superimposed basin in China have been continuously studied.(1) The fault-controlled carbonate reservoir and the ancient dolomite reservoir are two important types of reservoirs in the deep and ultra-deep marine carbonates. According to the formation origin, the large-scale fault-controlled reservoir can be further divided into three types:fracture-cavity reservoir formed by tectonic rupture, fault and fluid-controlled reservoir, and shoal and mound reservoir modified by fault and fluid. The Sinian microbial dolomites are developed in the aragonite-dolomite sea. The predominant mound-shoal facies, early dolomitization and dissolution, acidic fluid environment, anhydrite capping and overpressure are the key factors for the formation and preservation of high-quality dolomite reservoirs.(2) The organic-rich shale of the marine carbonate strata in the superimposed basins of central and western China are mainly developed in the sedimentary environments of deep-water shelf of passive continental margin and carbonate ramp. The tectonic-thermal system is the important factor controlling the hydrocarbon phase in deep and ultra-deep reservoirs, and the reformed dynamic field controls oil and gas accumulation and distribution in deep and ultra-deep marine carbonates.(3) During the development of high-sulfur gas fields such as Puguang, sulfur precipitation blocks the wellbore. The application of sulfur solvent combined with coiled tubing has a significant effect on removing sulfur blockage. The integrated technology of dual-medium modeling and numerical simulation based on sedimentary simulation can accurately characterize the spatial distribution and changes of the water invasion front.Afterward, water control strategies for the entire life cycle of gas wells are proposed, including flow rate management, water drainage and plugging.(4) In the development of ultra-deep fault-controlled fractured-cavity reservoirs, well production declines rapidly due to the permeability reduction, which is a consequence of reservoir stress-sensitivity. The rapid phase change in condensate gas reservoir and pressure decline significantly affect the recovery of condensate oil. Innovative development methods such as gravity drive through water and natural gas injection, and natural gas drive through top injection and bottom production for ultra-deep fault-controlled condensate gas reservoirs are proposed. By adopting the hierarchical geological modeling and the fluid-solid-thermal coupled numerical simulation, the accuracy of producing performance prediction in oil and gas reservoirs has been effectively improved. 展开更多
关键词 deep and ultra-deep marine carbonate mechanisms of hydrocarbon accumulation reef-beach facies high-sulfur sour gas reservoirs ultra-deep fault-controlled fractured-cavity reservoir wellbore sulfur deposition fluid-solid-thermal numerical simulation
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A Novel Fracturing Fluid with High-Temperature Resistance for Ultra-Deep Reservoirs
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作者 Lian Liu Liang Li +2 位作者 Kebo Jiao Junwei Fang Yun Luo 《Fluid Dynamics & Materials Processing》 EI 2024年第5期975-987,共13页
Ultra-deep reservoirs play an important role at present in fossil energy exploitation.Due to the related high temperature,high pressure,and high formation fracture pressure,however,methods for oil well stimulation do ... Ultra-deep reservoirs play an important role at present in fossil energy exploitation.Due to the related high temperature,high pressure,and high formation fracture pressure,however,methods for oil well stimulation do not produce satisfactory results when conventional fracturing fluids with a low pumping rate are used.In response to the above problem,a fracturing fluid with a density of 1.2~1.4 g/cm^(3)was developed by using Potassium formatted,hydroxypropyl guanidine gum and zirconium crosslinking agents.The fracturing fluid was tested and its ability to maintain a viscosity of 100 mPa.s over more than 60 min was verified under a shear rate of 1701/s and at a temperature of 175℃.This fluid has good sand-carrying performances,a low viscosity after breaking the rubber,and the residue content is less than 200 mg/L.Compared with ordinary reconstruction fluid,it can increase the density by 30%~40%and reduce the wellhead pressure of 8000 m level reconstruction wells.Moreover,the new fracturing fluid can significantly mitigate safety risks. 展开更多
关键词 ultra-deep reservoir high-temperature resistance weighted fracturing fluid guanidine gum potassium formatted
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Control of Earth system evolution on the formation and enrichment of marine ultra-deep petroleum in China
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作者 ZHANG Shuichang WANG Huajian +3 位作者 SU Jin WANG Xiaomei HE Kun LIU Yuke 《Petroleum Exploration and Development》 SCIE 2024年第4期870-885,共16页
Taking the Paleozoic of the Sichuan and Tarim basins in China as example,the controlling effects of the Earth system evolution and multi-spherical interactions on the formation and enrichment of marine ultra-deep petr... Taking the Paleozoic of the Sichuan and Tarim basins in China as example,the controlling effects of the Earth system evolution and multi-spherical interactions on the formation and enrichment of marine ultra-deep petroleum in China have been elaborated.By discussing the development of“source-reservoir-seal”controlled by the breakup and assembly of supercontinents and regional tectonic movements,and the mechanisms of petroleum generation and accumulation controlled by temperature-pressure system and fault conduit system,Both the South China and Tarim blocks passed through the intertropical convergence zone(ITCZ)of the low-latitude Hadley Cell twice during their drifts,and formed hydrocarbon source rocks with high quality.It is proposed that deep tectonic activities and surface climate evolution jointly controlled the types and stratigraphic positions of ultra-deep hydrocarbon source rocks,reservoirs,and seals in the Sichuan and Tarim basins,forming multiple petroleum systems in the Ediacaran-Cambrian,Cambrian-Ordovician,Cambrian-Permian and Permian-Triassic strata.The matching degree of source-reservoir-seal,the type of organic matter in source rocks,the deep thermal regime of basin,and the burial-uplift process across tectonic periods collectively control the entire process from the generation to the accumulation of oil and gas.Three types of oil and gas enrichment models are formed,including near-source accumulation in platform marginal zones,distant-source accumulation in high-energy beaches through faults,and three-dimensional accumulation in strike-slip fault zones,which ultimately result in the multi-layered natural gas enrichment in ultra-deep layers of the Sichuan Basin and co-enrichment of oil and gas in the ultra-deep layers of the Tarim Basin. 展开更多
关键词 evolution of Earth system multi-spherical interaction Paleozoic marine ultra-deep petroleum Tarim Basin Sichuan Basin
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Research progress and development of deep and ultra-deep drilling fluid technology
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作者 SUN Jinsheng YANG Jingbin +2 位作者 BAI Yingrui LYU Kaihe LIU Fengbao 《Petroleum Exploration and Development》 SCIE 2024年第4期1022-1034,共13页
The research progress of deep and ultra-deep drilling fluid technology systematically reviewed,the key problems existing are analyzed,and the future development direction is proposed.In view of the high temperature,hi... The research progress of deep and ultra-deep drilling fluid technology systematically reviewed,the key problems existing are analyzed,and the future development direction is proposed.In view of the high temperature,high pressure and high stress,fracture development,wellbore instability,drilling fluid lost circulation and other problems faced in the process of deep and ultra-deep complex oil and gas drilling,scholars have developed deep and ultra-deep high-temperature and high-salt resistant water-based drilling fluid technology,high-temperature resistant oil-based/synthetic drilling fluid technology,drilling fluid technology for reservoir protection and drilling fluid lost circulation control technology.However,there are still some key problems such as insufficient resistance to high temperature,high pressure and high stress,wellbore instability and serious lost circulation.Therefore,the development direction of deep and ultra-deep drilling fluid technology in the future is proposed:(1)The technology of high-temperature and high-salt resistant water-based drilling fluid should focus on improving high temperature stability,improving rheological properties,strengthening filtration control and improving compatibility with formation.(2)The technology of oil-based/synthetic drilling fluid resistant to high temperature should further study in the aspects of easily degradable environmental protection additives with low toxicity such as high temperature stabilizer,rheological regulator and related supporting technologies.(3)The drilling fluid technology for reservoir protection should be devoted to the development of new high-performance additives and materials,and further improve the real-time monitoring technology by introducing advanced sensor networks and artificial intelligence algorithms.(4)The lost circulation control of drilling fluid should pay more attention to the integration and application of intelligent technology,the research and application of high-performance plugging materials,the exploration of diversified plugging techniques and methods,and the improvement of environmental protection and production safety awareness. 展开更多
关键词 deep and ultra-deep drilling high temperature resistant drilling fluid reservoir protection drilling fluid lost circulation control safety and environmental protection technical prospects
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Research on the strain gauge mounting scheme of track wheel force measurement system based on high-speed wheel/rail relationship test rig
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作者 Yuanwu Cai Bo Chen Chongyi Chang 《Railway Sciences》 2024年第4期503-513,共11页
Purpose-This paper aims to analyze the stress and strain distribution on the track wheel web surface and study the optimal strain gauge location for force measurement system of the track wheel.Design/methodology/appro... Purpose-This paper aims to analyze the stress and strain distribution on the track wheel web surface and study the optimal strain gauge location for force measurement system of the track wheel.Design/methodology/approach-Finite element method was employed to analyze the stress and strain distribution on the track wheel web surface under varying wheel-rail forces.Locations with minimal coupling interference between vertical and lateral forces were identified as suitable for strain gauge installation.Findings-The results show that due to the track wheel web’s unique curved shape and wheel-rail force loading mechanism,both tensile and compressive states exit on the surface of the web.When vertical force is applied,Mises stress and strain are relatively high near the inner radius of 710 mm and the outer radius of 1110mmof the web.Under lateral force,high Mises stress and strain are observed near the radius of 670mmon the inner and outer sides of the web.As the wheel-rail force application point shifts laterally toward the outer side,the Mises stress and strain near the inner radius of 710 mm of the web gradually decrease under vertical force while gradually increasing near the outer radius of 1110 mm of the web.Under lateral force,the Mises stress and strain on the surface of the web remain relatively unchanged regardless of the wheel-rail force application point.Based on the analysis of stress and strain on the surface of the web under different wheel-rail forces,the inner radius of 870 mm is recommended as the optimal mounting location of strain gauges for measuring vertical force,while the inner radius of 1143 mm is suitable for measuring lateral force.Originality/value-The research findings provide valuable insights for determining optimal strain gauge locations and designing an effective track wheel force measurement system. 展开更多
关键词 Track wheel High-speed wheel/rail relationship test rig Instrumented wheelset Strain gauge Finite element
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RIG-Ⅰ、USP5在卵巢上皮性癌组织中的表达水平及预后意义
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作者 齐曼 芦秋彤 +3 位作者 刘少卿 张娟 崔艳萍 刘咏梅 《中南医学科学杂志》 CAS 2023年第2期277-280,共4页
目的 探讨维甲酸诱导基因-Ⅰ(RIG-Ⅰ)、泛素特异性肽酶5(USP5)在卵巢上皮性癌组织中的表达水平及预后意义。方法 选取98例卵巢上皮性癌组织样本为病例组,同期98例正常卵巢组织为对照组。免疫组化法测定两组RIG-Ⅰ、USP5表达水平。单因... 目的 探讨维甲酸诱导基因-Ⅰ(RIG-Ⅰ)、泛素特异性肽酶5(USP5)在卵巢上皮性癌组织中的表达水平及预后意义。方法 选取98例卵巢上皮性癌组织样本为病例组,同期98例正常卵巢组织为对照组。免疫组化法测定两组RIG-Ⅰ、USP5表达水平。单因素及多因素Cox回归分析RIG-Ⅰ、USP5表达水平与卵巢上皮性癌患者临床预后的关系。结果 病例组RIG-Ⅰ、USP5表达阳性率均高于对照组(P<0.05)。卵巢上皮性癌患者RIG-Ⅰ、USP5表达与肿瘤最大径、TNM分期、肿瘤浸润、分化程度、淋巴结转移、复发率相关(P<0.05)。RIG-Ⅰ、USP5阳性的卵巢上皮性癌患者5年生存率均分别低于RIG-Ⅰ、USP5阴性的卵巢上皮性癌患者(P<0.05)。单因素分析显示:肿瘤最大径≥2 cm、TNM分期Ⅲ~Ⅳ期、浸润深度≥1 cm、分化程度高、淋巴结转移、复发、RIG-Ⅰ阳性、USP5阳性的卵巢上皮性癌患者5年生存率分别低于肿瘤最大径<2 cm、TNM分期Ⅰ~Ⅱ期、浸润深度<1 cm/无浸润、分化程度低、无淋巴结转移、无复发、RIG-Ⅰ阴性、USP5阴性的卵巢上皮性癌患者(P<0.05)。多因素Cox回归分析显示:TNM分期Ⅲ~Ⅳ期、分化程度高、RIG-Ⅰ和USP5表达阳性均是影响卵巢上皮性癌患者预后的独立危险因素(P<0.05)。结论 RIG-Ⅰ、USP5与卵巢上皮性癌患者临床病理特征相关;RIG-Ⅰ、USP5阳性表达是卵巢上皮性癌预后危险因素。 展开更多
关键词 维甲酸诱导基因Ⅰ 泛素特异性肽酶5 卵巢上皮性癌
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猪肠道组织RIG-I的表达及其在猪传染性胃肠炎病毒致病机制中作用的初步研究 被引量:4
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作者 王文哲 李亮 +2 位作者 何豪杰 薛美 冯力 《中国预防兽医学报》 CAS CSCD 北大核心 2023年第2期161-168,共8页
为探究天然免疫分子维甲酸诱导基因Ⅰ(RIG-I)在猪肠道组织中的表达情况,评价其在猪传染性胃肠炎病毒(TGEV)致病机制中的作用。本研究经原核表达重组RIG-I蛋白(rRIG-1)并采用SDS-PAGE切胶纯化该蛋白,将其免疫大白兔制备兔RIG-I多克隆抗体... 为探究天然免疫分子维甲酸诱导基因Ⅰ(RIG-I)在猪肠道组织中的表达情况,评价其在猪传染性胃肠炎病毒(TGEV)致病机制中的作用。本研究经原核表达重组RIG-I蛋白(rRIG-1)并采用SDS-PAGE切胶纯化该蛋白,将其免疫大白兔制备兔RIG-I多克隆抗体,采用间接ELISA检测抗体效价。结果显示获得了效价可达1∶64000的兔RIG-I多克隆抗体。利用该抗体经western blot检测SPF猪各肠道组织中RIG-I的表达。结果显示,制备的多克隆抗体可与猪各肠道中的RIG-I反应,且RIG-I在SPF猪空肠中的表达量最高。利用该抗体经western blot检测TGEV感染的ST细胞及猪空肠中RIG-I的表达水平。结果显示,TGEV感染后ST细胞中RIG-I的表达水平极显著高于空白对照细胞(P<0.01);与正常SPF猪空肠相比,TGEV感染的SPF猪空肠组织中RIG-I蛋白的表达水平明显提高。表明TGEV感染后能够刺激细胞内源性及感染猪肠道组织中RIG-I蛋白的表达水平。将本研究构建的重组质粒pCAGGS-RIG-I-flag和RIG-I干扰RNA(siRIG-I)分别转染ST细胞,采用western blot鉴定RIG-I的过表达和敲低效果后,再利用TGEV感染,24 h后采用qPCR检测RIG-I对上述ST细胞中IFN-β转录水平的影响;收集细胞上清,采用TCID50方法测定病毒滴度。qPCR及病毒滴度的测定结果显示,与转染空载体的对照组相比,RIG-I过表达的ST细胞中IFN-β的转录水平极显著升高(P<0.001),且细胞上清中的病毒滴度下降约75%;与转染NCsiRNA的对照组相比,敲低RIG-I的ST细胞中IFN-β的转录水平极显著下降(P<0.001),且细胞上清中的病毒滴度升高约4.8倍。表明,TGEV感染细胞中的RIG-I能够促进细胞中IFN-β的转录,提高宿主细胞的抗病毒免疫反应,从而抑制病毒的复制。本研究结果证实RIG-I在介导TGEV诱导宿主细胞产生IFN的过程中发挥重要作用,该结果为深入探究TGEV的致病机制奠定了实验基础。 展开更多
关键词 维甲酸诱导基因I(rig-I) 多克隆抗体 猪传染性胃肠炎病毒 干扰素Β 致病机制
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Prediction Model of Drilling Costs for Ultra-Deep Wells Based on GA-BP Neural Network 被引量:1
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作者 Wenhua Xu Yuming Zhu +4 位作者 YingrongWei Ya Su YanXu Hui Ji Dehua Liu 《Energy Engineering》 EI 2023年第7期1701-1715,共15页
Drilling costs of ultra-deepwell is the significant part of development investment,and accurate prediction of drilling costs plays an important role in reasonable budgeting and overall control of development cost.In o... Drilling costs of ultra-deepwell is the significant part of development investment,and accurate prediction of drilling costs plays an important role in reasonable budgeting and overall control of development cost.In order to improve the prediction accuracy of ultra-deep well drilling costs,the item and the dominant factors of drilling costs in Tarim oilfield are analyzed.Then,those factors of drilling costs are separated into categorical variables and numerous variables.Finally,a BP neural networkmodel with drilling costs as the output is established,and hyper-parameters(initial weights and bias)of the BP neural network is optimized by genetic algorithm(GA).Through training and validation of themodel,a reliable prediction model of ultra-deep well drilling costs is achieved.The average relative error between prediction and actual values is 3.26%.Compared with other models,the root mean square error is reduced by 25.38%.The prediction results of the proposed model are reliable,and the model is efficient,which can provide supporting for the drilling costs control and budget planning of ultra-deep wells. 展开更多
关键词 ultra-deep well drilling costs cost estimation BP neural network genetic algorithm
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ZNF205通过靶向RIG-I正调控RLR抗病毒信号通路
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作者 钟霓 王晨 +1 位作者 翁光秀 许亮国 《免疫学杂志》 CAS CSCD 北大核心 2023年第8期708-713,共6页
目的探究ZNF205(zinc finger protein 205)对RIG-I(retinoic acid-inducible gene I)介导的RLR(RIG-I like receptors,RLRs)抗病毒信号通路的影响。方法采用免疫共沉淀、免疫印迹、非变性聚丙烯酰胺凝胶电泳、双荧光素酶报告基因实验、... 目的探究ZNF205(zinc finger protein 205)对RIG-I(retinoic acid-inducible gene I)介导的RLR(RIG-I like receptors,RLRs)抗病毒信号通路的影响。方法采用免疫共沉淀、免疫印迹、非变性聚丙烯酰胺凝胶电泳、双荧光素酶报告基因实验、实时荧光定量PCR、病毒空斑等实验探索ZNF205在RLRs信号通路中的作用。结果ZNF205与RIG-I相互作用。过表达ZNF205促进了SeV诱导的IRF3(interferon regulatory factor 3)的二聚化,也增强了RIG-I-N(aa1-284)介导或SeV诱导的IFN-β(interferon beta)启动子的活性,同时还增强了SeV诱导的IFN-β的转录。病毒空斑实验结果显示过表达ZNF205能够抑制病毒的复制。研究结果还表明,ZNF205促进RIG-I的泛素化修饰以及RIG-I的K63泛素化修饰。结论ZNF205在RLR抗病毒先天免疫信号通路中起正调控作用。 展开更多
关键词 ZNF205 rig-I 泛素化 RLR信号抗病毒信号通路
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Research progress and key issues of ultra-deep oil and gas exploration in China
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作者 HE Dengfa JIA Chengzao +8 位作者 ZHAO Wenzhi XU Fengyin LUO Xiaorong LIU Wenhui TANG Yong GAO Shanlin ZHENG Xiujuan LI Di ZHENG Na 《Petroleum Exploration and Development》 SCIE 2023年第6期1333-1344,共12页
Based on the recent oil and gas discoveries and geological understandings on the ultra-deep strata of sedimentary basins, the formation and occurrence of hydrocarbons in the ultra-deep strata were investigated with re... Based on the recent oil and gas discoveries and geological understandings on the ultra-deep strata of sedimentary basins, the formation and occurrence of hydrocarbons in the ultra-deep strata were investigated with respect to the processes of basin formation, hydrocarbon generation, reservoir formation and hydrocarbon accumulation, and key issues in ultra-deep oil and gas exploration were discussed. The ultra-deep strata in China underwent two extensional-convergent cycles in the Meso-Neoproterozoic Era and the Early Paleozoic Era respectively, with the tectonic-sedimentary differentiation producing the spatially adjacent source-reservoir assemblages. There are diverse large-scale carbonate reservoirs such as mound-beach, dolomite, karst fracture-vug, fractured karst and faulted zone, as well as over-pressured clastic rock and fractured bedrock reservoirs. Hydrocarbons were accumulated in multiple stages, accompanied by adjusting and finalizing in the late stage. The distribution of hydrocarbons is controlled by high-energy beach zone, regional unconformity, paleo-high and large-scale fault zone. The ultra-deep strata endow oil and gas resources as 33% of the remaining total resources, suggesting an important successive domain for hydrocarbon development in China. The large-scale pool-forming geologic units and giant hydrocarbon enrichment zones in ultra-deep strata are key and promising prospects for delivering successive discoveries. The geological conditions and enrichment zone prediction of ultra-deep oil and gas are key issues of petroleum geology. 展开更多
关键词 China ultra-deep oil and gas multi-cycle superimposed basin exploration progress exploration technology light oil natural gas
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Origin of gas condensate reservoir in Fuman Oilfield, Tarim Basin, NW China
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作者 WANG Qinghua 《Petroleum Exploration and Development》 SCIE 2023年第6期1295-1307,共13页
To understand the reservoir property and hydrocarbon accumulation conditions of the Middle and Upper Ordovician intraplatform shoal between ultra-deep main strike-slip faults in Fuman Oilfield of the Tarim Basin, Chin... To understand the reservoir property and hydrocarbon accumulation conditions of the Middle and Upper Ordovician intraplatform shoal between ultra-deep main strike-slip faults in Fuman Oilfield of the Tarim Basin, China, the main strike-slip faults in and around well FD1 in the basin were analyzed in terms of sedimentary facies, sequence stratigraphy, intraplatform shoal reservoir property, and oil and gas origins, based on drilling data. The Yingshan Formation intraplatform shoal between the main strike-slip faults is superimposed with low-order faults to form a new type of hydrocarbon play. Firstly, hydrocarbons generated from the Lower Cambrian Yuertusi Formation source rocks vertically migrated into the second member of Yingshan Formation through the main strike-slip faults, and then migrated laterally until they were accumulated. A small amount of oil from Well FD1 came from the Yuertusi Formation source rocks in the mature stage, and a large amount of gas originated from oil cracking in the ultra-deep reservoirs. Therefore, the secondary gas condensate reservoir in Well FD1 is characterized by high gas to oil ratio, dry gas (dryness coefficient being 0.970) and hybrid origin. This new type of hydrocarbon play characterized by intraplatform shoal and low-order fault suggests a prospect of continuous hydrocarbon-bearing area in Fuman Oilfield, which will expand the ultrap-deep oil and gas exploration in the oilfield. 展开更多
关键词 low-order fault intraplatform shoal ultra-deep Yingshan Formation oil cracked gas condensate gas Fuman Oilfield Tarim Basin
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Development of a Test Rig for Axial Strains Measurement in Automobile Wheel
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作者 Samuel Onoriode Igbudu David Abimbola Fadare 《Open Journal of Applied Sciences》 2023年第10期1764-1777,共14页
In automobile wheel application, a test rig is vital and used to simulate conditions of the wheel in service in order to affirm the safety and reliability of the wheel. The present work designed a test rig for measuri... In automobile wheel application, a test rig is vital and used to simulate conditions of the wheel in service in order to affirm the safety and reliability of the wheel. The present work designed a test rig for measuring axial strains in automobile wheel. The wheel used was a five-arm wheel (6JX14H2;ET 42) and Tyre (175 × 65 R 14). Experimental (EXP) test was carried out, with a radial load of 4750 N and inflation pressure of 0.3 MPa, to measure the axil strains which were converted to maximum principal strain values and, compared with data from Finite Element Analysis (FEA) using Creo-Element/Pro 5.0 at wheel’s contact angles of 90 degree (FEA 90 deg), 40 degree (FEA 40 deg) and 30.25 degree (FEA 30.25 deg), respectively. Results show that at the wheel’s point of contact with the ground, maximum principal strain values were highest at the inboard bead seat with a value of about 5.69 × 10<sup>-4</sup> mm/mm, followed by the values at the well of about 5.66 × 10<sup>-4</sup> mm/mm. The value at the outboard bead seat was least at about 2.22 × 10<sup>-4</sup> mm/mm, which was due to the presence of spikes at this location that tends to resist imposed radial loads. However, the highest mean maximum principal strain values at the locations of inboard, well and outboard, were about 2.11 × 10<sup>-4</sup> mm/mm, 3.78 × 10<sup>-4</sup> mm/mm and .99 × 10<sup>-4</sup> mm/mm, respectively. With the highest single value of about 5.69 × 10<sup>-4</sup> mm/mm, the inboard bead seat was the most strained location of the wheel. Overall results showed that all values of maximum principal strains were below the threshold value of about 1 × 10<sup>-2</sup> mm/mm. The values obtained for EXP and FEA could be said to be in close agreement when compared with the threshold value. With this in mind, the rig is recommended for use in related experimental procedures. 展开更多
关键词 Test rig STRAIN Contact Angle Radial Load Inflation Pressure
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Finite Element Analysis of Jumbo Rig Assembly for Tunneling
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作者 YAO Haiping 《International Journal of Plant Engineering and Management》 2023年第1期59-64,共6页
This paper describes the force acting the assembly of the jumbo rig for tunneling.The finite element analysis is used to calculate this machine structure on its different working states.
关键词 jumbo rig for tunneling fea STRESS DEFORMATION failure analysis
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中国石油井下作业数字化监督发展问题与对策 被引量:1
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作者 张绍辉 黄伟和 +5 位作者 毕国强 张晓辉 张建利 文虎成 李海军 田涛 《石油工业技术监督》 2024年第5期1-7,共7页
阐述了中国石油井下作业数字化监督在监督人员数字化管理、施工队伍数字化监督、施工作业数字化监督、井场环境数字化监督4个方面的发展现状。分析了井下作业数字化监督发展面临数字化监督管理制度不健全、监督数据标准不统一、数字化... 阐述了中国石油井下作业数字化监督在监督人员数字化管理、施工队伍数字化监督、施工作业数字化监督、井场环境数字化监督4个方面的发展现状。分析了井下作业数字化监督发展面临数字化监督管理制度不健全、监督数据标准不统一、数字化智能监督技术不完善、缺少统一的数字化智能监督平台、监督数据价值挖掘不深入5个方面的主要问题。提出了制定井下作业数字化监督管理制度、建立井下作业监督数据标准、攻关数字化智能监督关键技术、研发井下作业数字化智能监督平台、提升监督大数据分析应用水平5项数字化监督发展对策。展望了井下作业数字化监督建设发展后在提升监督人员履职效率、降低监督人力资源成本、提升工程质量、保障施工安全与环保4个方面的预期成效。 展开更多
关键词 井下作业 数字化监督 数据标准 修井机 视频监控 监督平台
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基于整车路面谱的副车架开裂台架试验及仿真
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作者 潘公宇 徐锐 杨晓峰 《郑州大学学报(工学版)》 北大核心 2024年第1期29-33,63,共6页
针对前悬架稳定杆与副车架连接处在整车试验场耐久测试过程中出现的疲劳破坏问题,通过对稳定杆进行贴片标定,获得了试验场环境下稳定杆的连杆力和稳定杆扭转相对位移的强化耐久路谱,设计搭建了稳定杆副车架系统的物理台架,并按照耐久性... 针对前悬架稳定杆与副车架连接处在整车试验场耐久测试过程中出现的疲劳破坏问题,通过对稳定杆进行贴片标定,获得了试验场环境下稳定杆的连杆力和稳定杆扭转相对位移的强化耐久路谱,设计搭建了稳定杆副车架系统的物理台架,并按照耐久性规范进行了台架试验。试验结果表明:该台架试验装置能良好地再现整车耐久路试下的断裂位置,疲劳寿命相对于整车耐久路试的疲劳寿命偏差仅为2.5%。在此基础上,建立了稳定杆与副车架多体的仿真模型,设定了与系统试验台架相同的约束边界,通过虚拟迭代的方法及准静态有限元疲劳寿命分析法对系统进行了仿真分析。仿真得到稳定杆连杆力和稳定杆扭转相对位移信号,与测试结果进行对比分析可以看出,时域下相位和幅值重合度良好,频域下的PSD谱重合度较高,穿级计数载荷统计基本一致,载荷相对损伤均在1附近,相关风险位置处的仿真疲劳寿命与整车耐久试验寿命偏差比为6.25%,获得了较高精度的风险位置载荷,实现了耐久风险位置的复现。最后,基于仿真模型对副车架风险位置处进行了结构优化设计,改进后的方案顺利通过了后续的台架试验和整车耐久路试。 展开更多
关键词 台架试验 耐久 虚拟迭代 疲劳仿真 相关性
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试验台约束对滑动轴承动特性识别精度的影响
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作者 陈润霖 唐杰 +3 位作者 徐帆 杜辰 崔亚辉 刘凯 《润滑与密封》 CAS CSCD 北大核心 2024年第3期189-195,共7页
在滑动轴承的动特性测试中,试验台参数对动特性测试精度有重要的影响。以某倒置式轴承动特性试验台为研究对象,基于轴承动力学正反问题,提出滑动轴承动特性系数识别精度的仿真评估方法,分析不同激振频率时试验台约束参数对轴承动特性系... 在滑动轴承的动特性测试中,试验台参数对动特性测试精度有重要的影响。以某倒置式轴承动特性试验台为研究对象,基于轴承动力学正反问题,提出滑动轴承动特性系数识别精度的仿真评估方法,分析不同激振频率时试验台约束参数对轴承动特性系数识别精度的影响规律,并对激振频率和约束参数的取值范围进行优选。结果表明:在较低激振频率的条件下,当约束刚度和约束阻尼取值较小时,动特性系数的识别精度受测试误差的影响不大,随着约束刚度和约束阻尼取值增大到一定值,动特性系数的识别精度受测试误差的影响迅速增大。针对研究的试验台,选择激振频率在30~300 Hz之间,选择试验台约束刚度小于试验轴承刚度的0.3%,试验台约束阻尼小于试验轴承阻尼的7%时,能够保证较好的轴承动特性系数的测试精度。 展开更多
关键词 滑动轴承试验台 约束刚度和阻尼 轴承动特性系数 识别精度
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