Bacterial infection is a major issue after artificial bone transplantation due to the absence of antibacterial function of bone scaffold,which seriously causes the transplant failure and even amputation in severe case...Bacterial infection is a major issue after artificial bone transplantation due to the absence of antibacterial function of bone scaffold,which seriously causes the transplant failure and even amputation in severe cases.In this study,oxygen vacancy(OV)defects Fe-doped Ti O2(OV-FeTiO2)nanoparticles were synthesized by nano TiO2and Fe3O4via high-energy ball milling,which was then incorporated into polycaprolactone/polyglycolic acid(PCLGA)biodegradable polymer matrix to construct composite bone scaffold with good antibacterial activities by selective laser sintering.The results indicated that OV defects were introduced into the core/shell-structured OV-FeTiO2nanoparticles through multiple welding and breaking during the high-energy ball milling,which facilitated the adsorption of hydrogen peroxide(H2O2)in the bacterial infection microenvironment at the bone transplant site.The accumulated H2O2could amplify the Fenton reaction efficiency to induce more hydroxyl radicals(·OH),thereby resulting in more bacterial deaths through·OH-mediated oxidative damage.This antibacterial strategy had more effective broad-spectrum antibacterial properties against Gram-negative Escherichia coli(E.coli)and Gram-positive Staphylococcus aureus(S.aureus).In addition,the PCLGA/OV-FeTiO2scaffold possessed mechanical properties that match those of human cancellous bone and good biocompatibility including cell attachment,proliferation and osteogenic differentiation.展开更多
TiB2/Al-30Si composites were fabricated via in-situ melt reaction under high-energy ultrasonic field. The microstructure and wear properties of the composite were investigated by XRD, SEM and dry sliding testing. The ...TiB2/Al-30Si composites were fabricated via in-situ melt reaction under high-energy ultrasonic field. The microstructure and wear properties of the composite were investigated by XRD, SEM and dry sliding testing. The results indicate that TiB2 reinforcement particles are uniformly distributed in the aluminum matrix under high-energy ultrasonic field. The morphology of the TiB2 particles is in circle-shape or quadrangle-shape, and the size of the particles is 0.1-1.5μm. The primary silicon particles are in quadrangle-shape and the average size of them is about 10μm. Hardness values of the Al-30Si matrix alloy and the TiB2/Al-30Si composites considerably increase as the high energy ultrasonic power increases. In particular, the maximum hardness value of the in-situ composites is about 1.3 times as high as that of the matrix alloy when the ultrasonic power is 1.2 kW, reaching 412 MPa. Meanwhile, the wear resistance of the in-situ TiB2/Al-30Si composites prepared under high-energy ultrasonic field is obviously improved and is insensitive to the applied loads of the dry sliding testing.展开更多
Under near ambient temperature and ultrasonication, nanocrystalline Zn(Oxin)2·2H2O (zinc 8-quinolinolate) was synthesized by solid state chemical reaction. The particle size distribution was relatively uniform, t...Under near ambient temperature and ultrasonication, nanocrystalline Zn(Oxin)2·2H2O (zinc 8-quinolinolate) was synthesized by solid state chemical reaction. The particle size distribution was relatively uniform, the morphology of the mare was ball like particle. The phase, particle size and morphology of the prepared nanocrystalline were characterized by powder X-ray diffraction (XRD), transmission electron microscopy (TEM) and electron diffraction (ED). The results show that the crystallite product has an average size of about 30 nm. The effects of a series of reaction conditions on the synthesis of Zn(Oxin)2·2H2O by solid state reaction were studied. During the synthesis of nanocrystalline Zn(Oxin)2·2H2O, the solid state reaction conditions such as changing reactant, matching proportion of reactant, adding inert substance, joining a little solvent or surface active solvent and grinding at different times may influence morphology, particle size and the size distribution of final products.展开更多
Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Me...Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Methods:Color Doppler ultrasound technique was adopted to detect the portal vein and blood flow change of patients with clonorchiasis;ELISA was used to determine the level of different serum inflammatory factors.The levels of serum total bilirubin,serum albumin and glutamic-pyruvic transaminase were detected by automatic biochemical analyzer.Western blot was used to determine the expression of proteins relevant to apoptosis.Results:Compared with the health control group,the trunk diameter of portal vein and the thickness of spleen,as well as the hepatic artery pulsation index of clonorchiasis patients increased obviously,the mean blood flow velocity of portal vein(P<0.05 or P<0.01) decreased.The content of total bilirubin and transaminase in plasma increased significantly,but albumin decreased(P<0.05).Levels of TNF-α,IL-6 and IFN-γ increased remarkably,and the level of every factor was significantly different among patients with Child-Pugh Ⅰ,Child-Pugh Ⅱ and Child-Pugh Ⅲ classification of liver function(P<0.05 or P<0.01).With the exacerbation of liver dysfunction,levels of TNF-α,IL-6 and IFN-γ gradually increased(P<0.05).Compared with the healthy control group,the expression quantity of apoptosis protein Fas,FasL,Bax and Caspase-3 increased significantly(P<0.05 or P<0.01),but Bcl-2 decreased(P<0.05).Conclusions:Changes of ultrasonic characteristics and liver dysfunction,caused by liver fluke infection,may be related to that both inflammatory response and apoptosis response have participated in the pathogenic process and liver damage course of clonorchiasis.展开更多
To develop more efficient chemical methods for the demineralization of organic pollutants from water bodies, which one was also mimic to the nature, a degradation of methylene blue by Fe(Ⅲ) and H 2O 2 in the absenc...To develop more efficient chemical methods for the demineralization of organic pollutants from water bodies, which one was also mimic to the nature, a degradation of methylene blue by Fe(Ⅲ) and H 2O 2 in the absence of light instead of Fe(Ⅱ) and H 2O 2 was studied. Results showed that use of Fe (Ⅲ) is more promising than Fe(Ⅱ). The present study reflects that Fenton reaction is more efficient, in the presence of a small amount of salicylic acid is added which is a one of the priority pollutant.展开更多
Magnetic field was tentatively introduced into Fenton reactions system for the degradation and discoloration of methyl blue as the represent of organic chemical dye, which was a bio-refractory organic pollutant in ind...Magnetic field was tentatively introduced into Fenton reactions system for the degradation and discoloration of methyl blue as the represent of organic chemical dye, which was a bio-refractory organic pollutant in industry wastewater. It was found that under optimal Fenton reaction conditions, with the assistant of magnetic field in Fenton reactions, the degradation rate of methyl blue, the decomposition rate of H2O2 and the conversion rate of Fe^2＋ were accelerated, the extent of them would be improved by the increase of magnetic field intensity. Meanwhile, the mineralization of methyl blue （CODer） was improved by over 10% with magnetic field.展开更多
In order to synthesize an improved adsorbent for heavy metal ions,we studied the condensation reaction of chitosan with salicylaldehyde in ethanol to form a Schiff base.The effect irradiating the reaction using an ult...In order to synthesize an improved adsorbent for heavy metal ions,we studied the condensation reaction of chitosan with salicylaldehyde in ethanol to form a Schiff base.The effect irradiating the reaction using an ultrasonic liquid processor was contrasted with conventional methods.The IR spectra of condensed chitosan prepared by the two methods showed that their molecular structures were identical.The reaction conditions,including solvents,ultrasonic power density and irradiation time,pH,and reactant ratio,were optimized by orthogonal design.A shorter reaction time and a higher product yield were obtained using ultrasonic-assisted synthesis compared with the traditional method.A condensation degree of 89.63% was achieved using the optimized conditions:i.e.ultrasonic irradiation at 180 W for 60 min;95% ethanol as the solvent,pH 4.0,and salicylaldehyde:chitosan ratio of 6:1.展开更多
The effect of heterogeneous Fenton reaction was studied on methylene blue（MB） and Nitrosomonas europaea（N. europaea） cells. Four Fenton systems were prepared and compared with each other, including Nickel Foam（NF...The effect of heterogeneous Fenton reaction was studied on methylene blue（MB） and Nitrosomonas europaea（N. europaea） cells. Four Fenton systems were prepared and compared with each other, including Nickel Foam（NF）/TiO2, NF/Bi2WO6, Ceramic foam（CM）/TiO2, and CM/Bi2WO6. The order of effect of fenton reaction ranked as NF/TiO2〉CM/TiO2〉NF/Bi2WO6〉CM/Bi2WO6. In acid or alkaline solution, the removal efficiency also decreased compared with neutral solution. With lower p H values, the nanoparticles were easier to break off from NF skeleton. Thus the synergetic effect of photocatalysis and fenton reaction can not take action. As for CM skeleton, the bond –Si-O-can bind with TiO2 or Bi2WO6. The membrane fluidity was used as an indicating parameter. After being treated by Fenton reaction, N. europaea surface was rougher than the native bacterium and the bulges on cell surface became irregular, which is attributed to change of lipopolysaccharide patches. Polarization of N. europaea cell membrane in acid medium increased more obvious than alkaline medium.展开更多
A novel, simple and convenient method for the determination of hydroxyl radicals isestablished. Hydroxyl radicals produced by Fenton reaction is trapped by spin trap reagent phenyl-t-butyl nitrone (PBN), and the free ...A novel, simple and convenient method for the determination of hydroxyl radicals isestablished. Hydroxyl radicals produced by Fenton reaction is trapped by spin trap reagent phenyl-t-butyl nitrone (PBN), and the free radical adduct of PBN can be detected by single sweeposcillopolarography, with its second order derivative cathodic wave at -0.52V vs SCE. Theoptimum experimental conditions for the detemination is discussed, and the scavenging effects ofsome compounds on OH was also studied.展开更多
基金supported by the following funds:The Natural Science Foundation of China(52275393,51935014,82072084)Hunan Provincial Natural Science Foundation of China(2021JJ20061)+4 种基金Jiangxi Provincial Natural Science Foundation of China(20224ACB204013)The Project of State Key Laboratory of Precision Manufacturing for Extreme Service PerformanceTechnology Innovation Platform Project of Shenzhen Institute of Information Technology 2020(PT2020E002)Guangdong Province Precision Manufacturing and Intelligent production education Integration Innovation Platform(2022CJPT019)Independent Exploration and Innovation Project of Central South University(1053320220553)。
文摘Bacterial infection is a major issue after artificial bone transplantation due to the absence of antibacterial function of bone scaffold,which seriously causes the transplant failure and even amputation in severe cases.In this study,oxygen vacancy(OV)defects Fe-doped Ti O2(OV-FeTiO2)nanoparticles were synthesized by nano TiO2and Fe3O4via high-energy ball milling,which was then incorporated into polycaprolactone/polyglycolic acid(PCLGA)biodegradable polymer matrix to construct composite bone scaffold with good antibacterial activities by selective laser sintering.The results indicated that OV defects were introduced into the core/shell-structured OV-FeTiO2nanoparticles through multiple welding and breaking during the high-energy ball milling,which facilitated the adsorption of hydrogen peroxide(H2O2)in the bacterial infection microenvironment at the bone transplant site.The accumulated H2O2could amplify the Fenton reaction efficiency to induce more hydroxyl radicals(·OH),thereby resulting in more bacterial deaths through·OH-mediated oxidative damage.This antibacterial strategy had more effective broad-spectrum antibacterial properties against Gram-negative Escherichia coli(E.coli)and Gram-positive Staphylococcus aureus(S.aureus).In addition,the PCLGA/OV-FeTiO2scaffold possessed mechanical properties that match those of human cancellous bone and good biocompatibility including cell attachment,proliferation and osteogenic differentiation.
基金Project(51174098)supported by the National Natural Science Foundation of ChinaProject(kjsmcx0903)supported by the Foundation of the Jiangsu Province Key Laboratory of Materials Tribology,China+2 种基金Project(1202015B)supported by the Postdoctoral Science Foundation of Jiangsu Province,ChinaProject(03)supported by the Undergraduate Practice-Innovation Training Foundation of Jiangsu University,ChinaProjects(GY2012020,GY2013032)supported by the Science and Technology Support Plan Project Foundation of Zhenjiang City,China
文摘TiB2/Al-30Si composites were fabricated via in-situ melt reaction under high-energy ultrasonic field. The microstructure and wear properties of the composite were investigated by XRD, SEM and dry sliding testing. The results indicate that TiB2 reinforcement particles are uniformly distributed in the aluminum matrix under high-energy ultrasonic field. The morphology of the TiB2 particles is in circle-shape or quadrangle-shape, and the size of the particles is 0.1-1.5μm. The primary silicon particles are in quadrangle-shape and the average size of them is about 10μm. Hardness values of the Al-30Si matrix alloy and the TiB2/Al-30Si composites considerably increase as the high energy ultrasonic power increases. In particular, the maximum hardness value of the in-situ composites is about 1.3 times as high as that of the matrix alloy when the ultrasonic power is 1.2 kW, reaching 412 MPa. Meanwhile, the wear resistance of the in-situ TiB2/Al-30Si composites prepared under high-energy ultrasonic field is obviously improved and is insensitive to the applied loads of the dry sliding testing.
基金Project (29631040) supported by the National Natural Science Foundation of China Project (200604) supported by the Scientific Research Foundation of Neijiang Teachers College, China
文摘Under near ambient temperature and ultrasonication, nanocrystalline Zn(Oxin)2·2H2O (zinc 8-quinolinolate) was synthesized by solid state chemical reaction. The particle size distribution was relatively uniform, the morphology of the mare was ball like particle. The phase, particle size and morphology of the prepared nanocrystalline were characterized by powder X-ray diffraction (XRD), transmission electron microscopy (TEM) and electron diffraction (ED). The results show that the crystallite product has an average size of about 30 nm. The effects of a series of reaction conditions on the synthesis of Zn(Oxin)2·2H2O by solid state reaction were studied. During the synthesis of nanocrystalline Zn(Oxin)2·2H2O, the solid state reaction conditions such as changing reactant, matching proportion of reactant, adding inert substance, joining a little solvent or surface active solvent and grinding at different times may influence morphology, particle size and the size distribution of final products.
基金supported by Trans-Century Training Programme Foundation for the Talents by the Ministry of Education (NO.NCET-11-0949)
文摘Objective:To discuss the liver function damage mechanism of patients with clonorchiasis by analyzing the ultrasound characteristics,liver function,change of the serum inflammatory factors and cell apoptosis factors.Methods:Color Doppler ultrasound technique was adopted to detect the portal vein and blood flow change of patients with clonorchiasis;ELISA was used to determine the level of different serum inflammatory factors.The levels of serum total bilirubin,serum albumin and glutamic-pyruvic transaminase were detected by automatic biochemical analyzer.Western blot was used to determine the expression of proteins relevant to apoptosis.Results:Compared with the health control group,the trunk diameter of portal vein and the thickness of spleen,as well as the hepatic artery pulsation index of clonorchiasis patients increased obviously,the mean blood flow velocity of portal vein(P<0.05 or P<0.01) decreased.The content of total bilirubin and transaminase in plasma increased significantly,but albumin decreased(P<0.05).Levels of TNF-α,IL-6 and IFN-γ increased remarkably,and the level of every factor was significantly different among patients with Child-Pugh Ⅰ,Child-Pugh Ⅱ and Child-Pugh Ⅲ classification of liver function(P<0.05 or P<0.01).With the exacerbation of liver dysfunction,levels of TNF-α,IL-6 and IFN-γ gradually increased(P<0.05).Compared with the healthy control group,the expression quantity of apoptosis protein Fas,FasL,Bax and Caspase-3 increased significantly(P<0.05 or P<0.01),but Bcl-2 decreased(P<0.05).Conclusions:Changes of ultrasonic characteristics and liver dysfunction,caused by liver fluke infection,may be related to that both inflammatory response and apoptosis response have participated in the pathogenic process and liver damage course of clonorchiasis.
文摘To develop more efficient chemical methods for the demineralization of organic pollutants from water bodies, which one was also mimic to the nature, a degradation of methylene blue by Fe(Ⅲ) and H 2O 2 in the absence of light instead of Fe(Ⅱ) and H 2O 2 was studied. Results showed that use of Fe (Ⅲ) is more promising than Fe(Ⅱ). The present study reflects that Fenton reaction is more efficient, in the presence of a small amount of salicylic acid is added which is a one of the priority pollutant.
基金support by the Natural Science Research Foundation of Jiangnan University(No.005796).
文摘Magnetic field was tentatively introduced into Fenton reactions system for the degradation and discoloration of methyl blue as the represent of organic chemical dye, which was a bio-refractory organic pollutant in industry wastewater. It was found that under optimal Fenton reaction conditions, with the assistant of magnetic field in Fenton reactions, the degradation rate of methyl blue, the decomposition rate of H2O2 and the conversion rate of Fe^2＋ were accelerated, the extent of them would be improved by the increase of magnetic field intensity. Meanwhile, the mineralization of methyl blue （CODer） was improved by over 10% with magnetic field.
基金Supported by Doctoral Discipline Special Foundation,Education Ministry of China (No.20050561014)the National High Technology Research and Development Program of China (863 Program) (No.2007AA100405)
文摘In order to synthesize an improved adsorbent for heavy metal ions,we studied the condensation reaction of chitosan with salicylaldehyde in ethanol to form a Schiff base.The effect irradiating the reaction using an ultrasonic liquid processor was contrasted with conventional methods.The IR spectra of condensed chitosan prepared by the two methods showed that their molecular structures were identical.The reaction conditions,including solvents,ultrasonic power density and irradiation time,pH,and reactant ratio,were optimized by orthogonal design.A shorter reaction time and a higher product yield were obtained using ultrasonic-assisted synthesis compared with the traditional method.A condensation degree of 89.63% was achieved using the optimized conditions:i.e.ultrasonic irradiation at 180 W for 60 min;95% ethanol as the solvent,pH 4.0,and salicylaldehyde:chitosan ratio of 6:1.
基金Funded by National Natural Science Foundation of Chinathe State Key Laboratory of Urban Water Resource and Environment(No.51208141)the National Key Research and Development Program of China(No.2016YFC0401102)
文摘The effect of heterogeneous Fenton reaction was studied on methylene blue（MB） and Nitrosomonas europaea（N. europaea） cells. Four Fenton systems were prepared and compared with each other, including Nickel Foam（NF）/TiO2, NF/Bi2WO6, Ceramic foam（CM）/TiO2, and CM/Bi2WO6. The order of effect of fenton reaction ranked as NF/TiO2〉CM/TiO2〉NF/Bi2WO6〉CM/Bi2WO6. In acid or alkaline solution, the removal efficiency also decreased compared with neutral solution. With lower p H values, the nanoparticles were easier to break off from NF skeleton. Thus the synergetic effect of photocatalysis and fenton reaction can not take action. As for CM skeleton, the bond –Si-O-can bind with TiO2 or Bi2WO6. The membrane fluidity was used as an indicating parameter. After being treated by Fenton reaction, N. europaea surface was rougher than the native bacterium and the bulges on cell surface became irregular, which is attributed to change of lipopolysaccharide patches. Polarization of N. europaea cell membrane in acid medium increased more obvious than alkaline medium.
文摘A novel, simple and convenient method for the determination of hydroxyl radicals isestablished. Hydroxyl radicals produced by Fenton reaction is trapped by spin trap reagent phenyl-t-butyl nitrone (PBN), and the free radical adduct of PBN can be detected by single sweeposcillopolarography, with its second order derivative cathodic wave at -0.52V vs SCE. Theoptimum experimental conditions for the detemination is discussed, and the scavenging effects ofsome compounds on OH was also studied.
