期刊文献+
共找到560篇文章
< 1 2 28 >
每页显示 20 50 100
Ganoboninketal C from Ganoderma boninense improves the efficacy of CDDP-based chemotherapy through inhibiting translesion DNA synthesis
1
作者 Xiaolu Ma Fei Yang +11 位作者 Ke Ma Hongyan Shen Junjie Han Kai Wang Yeran Yang Jiawei Zhu Ruiyuan An Qilin Wang Tie-Shan Tang Bo Zhou Hongwei Liu Caixia Guo 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第5期2982-2992,共11页
Translesion DNA synthesis(TLS)can bypass DNA lesions caused by chemotherapeutic drugs,which usually result in drug resistance.Given its key role in mutagenesis and cell survival after DNA damage,inhibition of the TLS ... Translesion DNA synthesis(TLS)can bypass DNA lesions caused by chemotherapeutic drugs,which usually result in drug resistance.Given its key role in mutagenesis and cell survival after DNA damage,inhibition of the TLS pathway has emerged as a potential target for improving the efficacy of DNA-damaging agents such as cisplatin(CDDP),a widely used anticancer agent.Unfortunately,few suitable natural TLS inhibitors have been reported.Here,we found that a triterpenoid compound Ganoboninketal C(26-3)from Ganoderma boninense,a traditional Chinese medicine,can impair CDDP-induced TLS polymerase eta(Polη)focus formation,PCNA monoubiquitination as well as mutagenesis.Moreover,26-3 can significantly sensitize tumor cells to CDDP killing and reduce the proportion of cancer stem cells in AGS and promote apoptosis after CDDP exposure.Interestingly,26-3 can also sensitize tumor cells to Gefitinib therapy.Mechanistically,through RNA-seq analysis,we found that 26-3 could abrogate the CDDP-induced upregulation of Polηand PIDD(p53-induced protein with a death domain),2 known factors promoting TLS pathway.Furthermore,we found that activating transcription factor 3 is a potential novel TLS modulator.Taken together,we have identified a natural TLS inhibitor 26-3,which can be potentially used as an adjuvant to improve clinical efficacy. 展开更多
关键词 Ganoderma boninense Ganoboninketal C Cisplatin chemotherapy Translesion dna synthesis
下载PDF
DNA存储系统中的数据写入 被引量:1
2
作者 张宣梁 李青婷 王飞 《合成生物学》 CSCD 北大核心 2024年第5期1125-1141,共17页
世界的数字化给人们的生活带来了极大的变化,但与此同时,史无前例的数据激增使得信息存储面临的挑战日益严峻。随着全球数据总量的指数级增长,传统存储介质将无法满足数字化带来的存储需求。使用DNA分子作为基本载体的信息存储展现出高... 世界的数字化给人们的生活带来了极大的变化,但与此同时,史无前例的数据激增使得信息存储面临的挑战日益严峻。随着全球数据总量的指数级增长,传统存储介质将无法满足数字化带来的存储需求。使用DNA分子作为基本载体的信息存储展现出高存储密度、低维护成本和易于化学修饰等独特优势。DNA存储主要包括编码、写入、保存、检索、读取和解码六个主要步骤,其中数据的写入是实现DNA存储功能的基础。本文首先介绍DNA存储系统中体外写入数据的策略方法,主要分为将数据写入DNA序列和写入DNA结构两个部分,接着概述体内写入数据技术的发展,最后将讨论DNA存储系统中数据写入面临的写入成本高、写入速度慢等挑战,并对大规模合成高纯度DNA、改进生物酶等具有前景的应用技术进行展望。 展开更多
关键词 dna存储 dna合成 核酸序列 dna纳米技术 框架核酸材料
下载PDF
Eukaryotic DNA damage tolerance and translesion synthesis through covalent modifications of PCNA 被引量:7
3
作者 Parker L Andersen Fang Xu Wei Xiao 《Cell Research》 SCIE CAS CSCD 2008年第1期162-173,共12页
In addition to well-defined DNA repair pathways, all living organisms have evolved mechanisms to avoid cell death caused by replication fork collapse at a site where replication is blocked due to disruptive covalent m... In addition to well-defined DNA repair pathways, all living organisms have evolved mechanisms to avoid cell death caused by replication fork collapse at a site where replication is blocked due to disruptive covalent modifications of DNA. The term DNA damage tolerance (DDT) has been employed loosely to include a collection of mechanisms by which cells survive replication-blocking lesions with or without associated genomic instability. Recent genetic analyses indicate that DDT in eukaryotes, from yeast to human, consists of two parallel pathways with one being error-free and another highly mutagenic. Interestingly, in budding yeast, these two pathways are mediated by sequential modifications of the proliferating cell nuclear antigen (PCNA) by two ubiquitination complexes Rad6-Rad18 and Mms2-Ubc13-Rad5. Damage-induced monoubiquitination of PCNA by Rad6-Rad18 promotes translesion synthesis (TLS) with increased mutagenesis, while subsequent polyubiquitination of PCNA at the same K164 residue by Mms2-Ubc13-Rad5 promotes error-free lesion bypass. Data obtained from recent studies suggest that the above mechanisms are conserved in higher eukaryotes. In particular, mammals contain multiple specialized TLS polymerases. Defects in one of the TLS polymerases have been linked to genomic instability and cancer. 展开更多
关键词 dna damage tolerance translesion synthesis Y-family polymerase UBIQUITINATION SUMOYLATION PCNA
下载PDF
THE EFFECT OF ACTIVE COMPONENTS OF LYCIUM BARBARUM AND GARLIC (LB-GO) ON THE SYNTHESIS OF DNA AND ULTRASTRUCTURE OF U_(14) CERVIX CANCER CELLS IN MICE 被引量:1
4
作者 胡庆和 高天顺 +4 位作者 赵承军 张焱 谢锦玉 沈联慈 刘铭福 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1994年第4期266-273,共8页
Mice, inoculated with U_(14) ascitic type cervix cancer cells, were administered with effective components of Lycium barbarum (LB) and garlic (GO).On the 4th day after i.p.administration, general condition of the anim... Mice, inoculated with U_(14) ascitic type cervix cancer cells, were administered with effective components of Lycium barbarum (LB) and garlic (GO).On the 4th day after i.p.administration, general condition of the animals were found improved.Examination of ascitic fluid revealed damage of the cancer cells, blanching of fluorescence staining of DNA and RNA, and the cancer cells besieged by large numbers of macrophages and leucocytes. Flow cell metric (FCM) analysis found: accumulation of cells of G_1 stage. Ultrastructure study disclosed: swelling of mitochondria in cytoplasm and damage of mitochondrial crests even with cavity formation, enlargement and degranulation of rough ER. It seemed the effect of LBGO was affirmable. It was postulated that macrophages,being activated by LB, came in close contact with the cancer cells giving rise to carcinolysis. In addition to the direct but transient killing effect of GO, the anti-cancer results could be greatly enhanced. 展开更多
关键词 Lycium barbarum GARLIC U_(14) Cervix cancer cell dna synthesis Ultrastructure.
下载PDF
HYPOXIA AND ENDOTHELIN-1 STIMULATE DNA SYNTHESIS OF PULMONARY ARTERY SMOOTH MUSCLE CELLS 被引量:2
5
作者 冯晓东 蔡英年 《Chinese Medical Sciences Journal》 CAS CSCD 1996年第1期28-31,共4页
Hypoxia and endothelin-1 (ET-1) are associated with constriction of pulmonary vasculature both in vivo and in vitro. However, the role of hypoxia and ET-1 in the vascular remodelling during the development of pulmonar... Hypoxia and endothelin-1 (ET-1) are associated with constriction of pulmonary vasculature both in vivo and in vitro. However, the role of hypoxia and ET-1 in the vascular remodelling during the development of pulmonary hypertension is unclear. This study demonstrated that ET-1(0. 1 nmol/L to 100 nmol/L) increased the [ ̄3H]thymidine uptake in a dose-dependent manner in cultured bovine pulmonary artery smooth muscle cells(PASMC). which was enhanced by exposing PASMC to hypoxia (2%O2, 93%N2,5%CO2 ). BQ123, the specific antagonist of endothelin receptor subtype A,eliminated the ET-1 medicated proliferation of PASMC and the cooperative effect of hypoxia. Some dilatory drugs could inhibite the mitogenic effect of ET-1. We also observed that hypoxia significantly increased [ ̄3H] thymidine uptake in PASMC without ET-1 and BQ123 could inhibite this effect. Radioimmunoassay suggested that there was an autocrine of ET-1 in cultured PASMC which was enhanced by hypoxia significantly. 展开更多
关键词 HYPOXIA ENDOTHELIN-1 dna synthesis
下载PDF
EFFECT OF GLYCYRRHETINIC ACID ON DNA DAMAGE AND UNSCHEDULED DNA SYNTHESIS INDUCED BY BENZO(α)PYRENE 被引量:1
6
作者 陈晓光 韩锐 《Chinese Medical Sciences Journal》 CAS CSCD 1995年第1期16-19,共4页
Glyeyrrhetinic acid (GA) is an active component of Glycyrrhiza uraleusis fisch,In this study,GA was found to inhibit ear edema and ornithine decarboxylase (ODC)activity induced by croton oil in mice. GA could also pro... Glyeyrrhetinic acid (GA) is an active component of Glycyrrhiza uraleusis fisch,In this study,GA was found to inhibit ear edema and ornithine decarboxylase (ODC)activity induced by croton oil in mice. GA could also protect rapid DNA damage and decrease the unscheduled DNA synthesis induced by benzo(α)pyrene. The results demonstrate that GA has a potential cancer chemopreventive activity. 展开更多
关键词 glycyrrhetinic acid dna damage protection unscheduled dna synthesis
下载PDF
Effects of Nerve Growth Factor on Proliferation and DNA Synthesis of Cultured Human Fetal Retinal Pigment Epithelium Cells 被引量:2
7
作者 LiWS WenJ 《Eye Science》 CAS 2002年第1X期45-48,49,共5页
Objective: To investigate the effects of nerve growth factor(NGF)on proliferation and DNAthesis of cultured human fetal retinal pigment epithelium (RPE)cells in vitro.Methods: Primary culture and subculture of human f... Objective: To investigate the effects of nerve growth factor(NGF)on proliferation and DNAthesis of cultured human fetal retinal pigment epithelium (RPE)cells in vitro.Methods: Primary culture and subculture of human fetal retinal pigment epithelium cellswere established in vitro first. Cultured RPE cells were treated with NGF by variousconcentrations 0μg/L, 50μg/L, 100μg/L, 200μg/L and 300μg/L(final concentration)for 48 hs.After 48 hs, cells proliferation was measured with methyl thiazolyl tetrazolium(MTT)assay method and the amount of DNA was determined by the absorbance at 280nm of nucleic acid & protein analysis.Results: The A values of 100 μg/L, 200 μg/L, 300 μg/L NGF was(0. 213 7 ± 0. 23 3),(0. 218 8 ±0. 018 1), (0. 232 2 ±0. 016 4) as compared with(0. 189 7 ±0. 015 2) of Avalue of 0 μg/L NGF respectively, q value was 3.63,4.40, 6. 42 and P value was0. 015, 0. 000, 0. 000(q-test). The DNA concentrations of 100 μg/L, 200 μg/L, 300μg/L and 400 μg/L NGF was (981. 220 4 ± 123.535 7), (1 375. 848 4 ±244. 471 8),(1 658.707 1 ± 176. 938 1), (2 353.086 3 ±609. 906 4) μg/ml as compared with(666. 818 8 ± 141. 330 2) μg/ml of DNA concentration of 0 μg/L NGF respectively, qvalue was 3.63,8.20,11.47,19.46, P value was 0. 024,0. 000,0. 000,0. 000 (q-test).Conclusion: The data suggested that NGF could stimulate the proliferation and DNAsynthesis of cultured of hRPE cells in vitro in a dose-dependent manner. 展开更多
关键词 眼色素上皮 神经生长因子 增殖 dna合成
下载PDF
Synthesis, Crystal Structure, and Thermal Stability of a Dibutyltin Complex {[4-Et_2NC_6H_3(O)C=NC_6H_3(O)-5-NO_2](n-Bu_2Sn)}_2 and Its Interaction with DNA
8
作者 张志坚 邝代治 +3 位作者 蒋伍玖 庾江喜 朱小明 张复兴 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2014年第9期1319-1325,共7页
The Schiff base organotin(IV) complex {[4-Et2NC6H3(O)C=NC6H3(O)-5-NO2](n- Bu2Sn)}2 has been synthesized via the reaction between 4-(diethylamino) salicylaldehyde-2-arnino- 4-nitrophenol Schiff base (H2L) a... The Schiff base organotin(IV) complex {[4-Et2NC6H3(O)C=NC6H3(O)-5-NO2](n- Bu2Sn)}2 has been synthesized via the reaction between 4-(diethylamino) salicylaldehyde-2-arnino- 4-nitrophenol Schiff base (H2L) and dibutyltin oxide. Complex C1 has been characterized by IR, 1H NMR, 13C NMR spectra, and elemental analysis, and its crystal structure was determined by X-ray diffraction, It crystallizes in the monoclinic system, space group P21/n with a = 15.6559(8), b = 9.1657(5), c = 18.8351(10) ]A, β = 107.3440(10)°, Z = 4, V = 2579.9(2) A3, Dc = 1.442 Mg.m-3, μ(MoKa) = 1.025 mm-1, F(000) = 1152, R = 0.0250 and wR = 0.0633. The central Sn atom is coordinated in a hexadentate manner to assume a distorted octahedral configuration. Complex C1 was studied by TGA analysis in air atmosphere. The interaction between complex C1 and the herring sperm DNA was realized through the intercalation of the complex based on the studies by EB fluorescent probe. 展开更多
关键词 organotin complex synthesis crystal structure thermal stability dna
下载PDF
EFFECT OF ASCORBIC ACID ON DNA SYNTHESIS,INTRACELLULAR ACCUMULATION OF ADM AND ADM RESISTANCE OF TUMOR CELL LINES
9
作者 谢佐福 林贤东 +1 位作者 周冬梅 林声 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1998年第2期32-35,共4页
Objective: To determine the effect of ascorbic acid (AA) on DNA synthesis, intracellular accumulation of ADM and ADM resistance of tumor cell lines. Methods: K562, K562/ADM and KB cell lines were used to study the e... Objective: To determine the effect of ascorbic acid (AA) on DNA synthesis, intracellular accumulation of ADM and ADM resistance of tumor cell lines. Methods: K562, K562/ADM and KB cell lines were used to study the effect of ascorbic acid on DNA synthesis, intracellular accumulation of ADM and ADM resistance by fluid scintillometry, MTT method, spectrofluorophotometry and immunocytochemistry. Results: Results showed that AA was capable of inhibiting DNA synthesis of K562 and K562/ADM in a dosedependence fashion, but not KB cell line, and significantly reducing ADM sensitivity in K562 and KB cell lines, as well as potentiating obviously ADM resistance in K562/ADM cell line. Conclusion: These effects of AA may be closely correlated with significant elevation of intracellular accumulation of ADM in KB cell line, and significant reduction of that in K562 and K562/ADM cell lines but possibly not correlated with the expression of Pglycoprotein. 展开更多
关键词 Ascorbic acid dna synthesis Drug resistance Tumor cell lines Experimental therapy
下载PDF
THE STUDIES OF REVERSAL ON INHIBITING DNA SYNTHESIS AND CLONAL FORMATION OF HL-60 CELLS WITH HYPERTHERMIA
10
作者 陈协群 沈素芸 +1 位作者 胡盛惠 伍柏松 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1994年第4期248-251,共4页
It was confirmed that the damage of HL-60 cells caused by heating (42℃, 60min) was of heterogeueity.The partial recovery of colony survival and 3H-TdR incorporation from inhibited HL-60 cells was acquired through sh... It was confirmed that the damage of HL-60 cells caused by heating (42℃, 60min) was of heterogeueity.The partial recovery of colony survival and 3H-TdR incorporation from inhibited HL-60 cells was acquired through short-term (24h) Iiquid culture. The resultsindicated that inhibition of DNA synthesis and colonyformation of leukemic cells by hyperthermia was partlyreversible. Its clinical significance and pathogenicmechanism were also discussed. 展开更多
关键词 HYPERTHERMIA dna synthesis HL-60 cells Bone marrow Transplantation Autologous.
下载PDF
ENHANCEMENT OF DNA SYNTHESIS IN CULTURED ADULT RAT HEPATOCYTES BY 5-HT THROUGH STIMULATION OF 5-HT_2RECEPTOR
11
作者 符兆英 《Journal of Pharmaceutical Analysis》 CAS 1999年第1期15-19,共5页
Hepatocytes were isolated from livers of adult male SpragueDawley rats and cultured in Williams'E Medium with 3 H thymidine. The effect of 5hydroxytryptamine (5HT) was investigated through adding various concentra... Hepatocytes were isolated from livers of adult male SpragueDawley rats and cultured in Williams'E Medium with 3 H thymidine. The effect of 5hydroxytryptamine (5HT) was investigated through adding various concentrations (10-810-3 mol/L) of 5HT to the hepatocyte cultures in the presence or absence of epidermal growth factor (EGF) and insulin. The involvement of 5HT2 receptor was examined by adding a 5HT2 receptor antagonist, ketanserin (10-6 mol/L), to some of the cultures containing 5HT. The increment of DNA synthesis was measured by 3 H thymidine incorporation. The results showed that 5HT2 (10-6 mol/L) significantly (P<005) increased the amount of DNA synthesis induced by EGF and insulin in the cultured adult rat hepaptocytes. The effect of 5HT in enhancing DNA synthesis began to appear at a concentration between 10-7 and 10-6 mol/L and reached maximum at concentrations of 10-4 mol/L. The enhancement of DNA synthesis by 5HT was significantly (P<005) antagonized by ketanserin, suggesting that this effect of 5HT was mediated by 5HT2 receptor subtype. 展开更多
关键词 dna synthesis HEPATOCYTE 5hydroxytryptamine (5HT) 5HT2 receptor
下载PDF
Jak_1/STAT_3 pathway mediates the inhibition of lipoxin A_4 on TNF-α-induced DNA synthesis of glomerular mesangial cells in rats
12
作者 Shenghua Wu Chao LU +1 位作者 Ling Dong Ziqing Chen 《Journal of Nanjing Medical University》 2005年第5期223-226,共4页
Objective: To examine whether lipoxin A4 (LXA4) has an inhibitory effect on tumor necrosis factor-α(TNF-α-induced DNA synthesis of glomerular mesangial cells of rat, and explore the molecular mechanisms of LXA4 ... Objective: To examine whether lipoxin A4 (LXA4) has an inhibitory effect on tumor necrosis factor-α(TNF-α-induced DNA synthesis of glomerular mesangial cells of rat, and explore the molecular mechanisms of LXA4 action. Methods: Glomerular mesangial cells of rat were cultured and preincubated with LXA4 at different concentrations, and then treated with TNF-α( 10 ng/ml). DNA synthesis was assessed by the incorporation of [^3H]-thymidine in mesangial cells. Expression of cyclin E protein was determined by Western blotting analysis. Activities of signal transducers and activators of transcription-3 (STAT3) were analyzed by electrophoretic mobility shift assay (EMSA). Results: TNF-α-stimulated DNA synthesis of mesangial cells, upregulafion of cyclin E protein and STAT3 activities were inhibited by LXA4 in a dose-dependent manner. Conclusion: TNF-α-induced DNA synthesis of mesangial cells can be inhibited by TXA4 probably through the mechanism of Jak1/STAT3 pathway-dependent signal transduction. 展开更多
关键词 LIPOXIN tumor necrosis factor dna synthesis CYCLIN STAT mesangial cell
下载PDF
EFFECT OF HYPOXIA ON DNA SYNTHESIS AND C-MYC GENE EXPRESSION OF PULMONARY ARTERY SMOOTH MUSCLE CELLS 
13
作者 罗兰 李世强 蔡英年 《Chinese Medical Sciences Journal》 CAS CSCD 1996年第4期224-227,共4页
The neonate is particularly susceptible to the development of hypoxic pulmonary hypertension. The present study was undertaken to observe the effect of hypoxia on DNA synthesis and c-myc gene expression between newbor... The neonate is particularly susceptible to the development of hypoxic pulmonary hypertension. The present study was undertaken to observe the effect of hypoxia on DNA synthesis and c-myc gene expression between newborn calf and adult bovine PASMC in vilro. DNA synthesis measured by 3H- TdR incorporation was increased after hypoxic challenge for 24h. Hypoxia enhanced the increment in 3H-TdR incorporation induced by EGF. Northern blot analysis revealed that PASMC cultured in both normoxia and hypoxia expressed c- myc gene transcript of 2. 2Kb ,but there is a higher 2. 2Kb mRNA expression in hypoxic PASMC than that in normoxia. We speculate that newborn calf PASMC exhibited potential response to hypoxia than adult,which was augmented by EGF. Enhanced c-myc gene expression may lead to a great understanding of the mechanism of PASMC growth in the development of pulmonary hypertension. 展开更多
关键词 HYPOXIA dna synthesis c-myc gene pulmonary artery smooth muscle cell
下载PDF
Evaluation of DNA synthesis with carbon-11-labeled 4′-thiothymidine
14
作者 Jun Toyohara 《World Journal of Radiology》 CAS 2016年第9期799-808,共10页
In the cancer research field, the preferred method for evaluating the proliferative activity of cancer cells in vivo is to measure DNA synthesis rates. The cellular proliferation rate is one of the most important canc... In the cancer research field, the preferred method for evaluating the proliferative activity of cancer cells in vivo is to measure DNA synthesis rates. The cellular proliferation rate is one of the most important cancer characteristics, and represents the gold standard of pathological diagnosis. Positron emission tomography (PET) has been used to evaluate in vivo DNA synthetic activity through visualization of enhanced nucleoside metabolism. However, methods for the quantitative measurement of DNA synthesis rates have not been fully clarified. Several groups have been engaged in research on 4&#x02032;-[methyl-<sup>11</sup>C]-thiothymidine (<sup>11</sup>C-4DST) in an effort to develop a PET tracer that allows quantitative measurement of in vivo DNA synthesis rates. This mini-review summarizes the results of recent studies of the in vivo measurement of cancer DNA synthesis rates using <sup>11</sup>C-4DST. 展开更多
关键词 4′-[methyl-11C]-thiothymidine dna synthesis Cell proliferation TUMOR Positron emission tomography
下载PDF
STUDIES ON THE SYNTHESIS AND DNA-BINDING ABILITY OF ZINC FINGER MOTIF
15
作者 Xiao Yu HU Rui WANG Xiang Qun LI (State Key Laboratory of Applied Organic Chemistry. Department of Biology.Lanzhou University. Lanzhou. 730000) 《Chinese Chemical Letters》 SCIE CAS CSCD 1994年第12期1023-1026,共4页
Transcription factor SPI is a protcin present in mammalian cells that binds to GC box promoter clements of Gene and selectively activates mRNA synthesis. The gene contains functional recognition sites. It contains thr... Transcription factor SPI is a protcin present in mammalian cells that binds to GC box promoter clements of Gene and selectively activates mRNA synthesis. The gene contains functional recognition sites. It contains three continuous zinc finger motifs, which are believed being mctalloprotein structures that interact with DNA. We synthesized the second zine finger fragment of SP1 (SP1-ZF2) and its mutant (SP1-ZF2 / HT. E20→H. R23→T), we also synthesized the Cys-Cys loop (ZF6) and the His-His loop (ZF5) of SPI and linked the twoloops together using a β-turn structure to obtain a finger mimic analogue (ZF-15) by stepwise solid-phase technique. Atomic absorption studies show that SP 1-ZF2 and SP1-ZF2 / HT bind zinc cquimolarly, but ZF-15 docs not bind Zn anyway. The CD experiments demonstrate a significant change in secondary structure in the prescnce or absence of Zn to SP1-ZF2 and SP1-ZF2/ HT, but there is no change about ZF-15. Gcl-retardation clectrophoresis assays indicate that SP1-ZF2 binds to DNA sequence specifically in the presence of Zn, but SP1-ZF2 / HT docs not bind as SP 1-ZF2 did. We observed that a single zine finger like SP1-ZF2 is able to bind DNA sequence specifically. 展开更多
关键词 dna STUDIES ON THE synthesis AND dna-BINDING ABILITY OF ZINC FINGER MOTIF
下载PDF
DNA-有机分子杂化体的合成及应用研究
16
作者 赵晓换 《辽宁化工》 CAS 2024年第5期768-770,808,共4页
随着DNA化学修饰技术的发展,疏水分子和DNA通过固相反应合成了两亲性DNA有机杂化物,两亲性有机杂化物可以被视为新的构建块,然后在溶液中自发组装成各种纳米结构,组装体在药物输送、靶向递送及生物传感等领域有着重要的应用。论述了DNA... 随着DNA化学修饰技术的发展,疏水分子和DNA通过固相反应合成了两亲性DNA有机杂化物,两亲性有机杂化物可以被视为新的构建块,然后在溶液中自发组装成各种纳米结构,组装体在药物输送、靶向递送及生物传感等领域有着重要的应用。论述了DNA-有机分子杂化体的组装、合成方法以及在生物医药方面的研究进展。 展开更多
关键词 dna 有机分子 合成 组装体 应用
下载PDF
A novel strategy for in situ maskless synthesis of biochips:Self-driving micro-fluid porous type printing (SMPTP)
17
作者 Nong Yue He Ya Fei Guo +1 位作者 Song Li Jian-Xin Tang 《Chinese Chemical Letters》 SCIE CAS CSCD 2007年第1期111-114,共4页
A novel maskless technique, self-driving micro-fluid porous type printing (SMPTP), was reported to in situ synthesize oligonucleotide arrays on glass slide, which has the merits of low cost, high quality and simple ... A novel maskless technique, self-driving micro-fluid porous type printing (SMPTP), was reported to in situ synthesize oligonucleotide arrays on glass slide, which has the merits of low cost, high quality and simple craft. In SMPTP for fabricating gene- chips, porous fiber tubes with a number of nanometric or micron channels functioned as "active letters" and were assembled in designed patterns, which are identical to the distribution of monomers in each layer of the array, and four patterns were needed for each layer. By means of capillarity, the synthesis solution was automatically taken into porous tubes assembled in a printing plate and reached the surface. An oligonucleotide array of 160 features with four different 15-mer probes was in situ synthesized using this technique. The four specific oligonucleotide probes, including the matched and the mismatched by the fluorescent target sequence, gave obviously different hybridization fluorescent signals. 展开更多
关键词 TYPOGRAPHY OLIGONUCLEOTIDE In situ synthesis dna microarrays BIOCHIPS
下载PDF
Chemical and enzymatic synthesis and doning of human epidermal growth factor gene
18
作者 赵忠良 陈南春 +1 位作者 李飚 陈苏民 《Journal of Medical Colleges of PLA(China)》 CAS 1992年第2期146-148,157,共4页
The human epidermal growth factor gene was synthesized with DNA synthesizer andby enzymes.Computer assisted to design the gene sequence and the restriction enzyme sites,andto elhninate the repeated and self-complement... The human epidermal growth factor gene was synthesized with DNA synthesizer andby enzymes.Computer assisted to design the gene sequence and the restriction enzyme sites,andto elhninate the repeated and self-complementary sequences.So that multiple doning and gene ex-pression were available for the designed gene sequence.The whole gene was formed by annealing8 chemically synthesized oligodeoxynucleotide fragments,enzymatically filling up the 4 twenty-basespaces and ligating the nicks,and was inserted into EcoR I and HInc Ⅱ sites ofplasmid pUC12.The recombinant plasmid was transformed into E.coli JM103 and 200 whitedones were obtained by X-gal and ampicilin screening.The positive clones were isolated andcharacterized by restriction enzyme analysis and DNA sequencing. 展开更多
关键词 EPIDERMAL GROWTH FACTOR dna RECOMBINANT GENE synthesis human
下载PDF
Chemical Synthesis and Cloning of Overlapping Concatenated Human Atrial Natriuretic Factor Gene with Gene Engineering
19
作者 杨静华 项秉懿 +1 位作者 陈南春 陈苏民 《Journal of Medical Colleges of PLA(China)》 CAS 1989年第2期181-185,187,共6页
Eight oligonucleotide fragments were designed with the aid of a computer and synthesizedaccording to the amino add sequcnce of human atrial natriuretic factor(ANF).By means of an-nealing and ligation,these fragments w... Eight oligonucleotide fragments were designed with the aid of a computer and synthesizedaccording to the amino add sequcnce of human atrial natriuretic factor(ANF).By means of an-nealing and ligation,these fragments were assembled into an overlapping concatenator consisting oftwo ANF genes ligated by TGATG for termination and initiation of translation.Theconcatenator was omserted into plasmid pRC23 and the recobinant DNA was transformed into E.coli strain TAP106.Analysis by restriction enzyme mapping,hybridization and DNA sequenongshowed that the orientation and reading frame of the gene were correct. 展开更多
关键词 atrial NATRIURETIC factor PLASMID dna synthesis OVERLAPPING CONCATENATED GENE
下载PDF
LONG TERM SUPPLEMENTATION OF SE-ENRICHED MALT FOR THE HIGH RISK POPULATION OF LUNG CANCER: INHIBITION OF ULTRAVIOLET LIGHT AND BENZOaPYRENE INDUCED UNSCHEDULED AND SYNTHESIS OF PERIPHERAL LYMPHOCYTES
20
作者 陈望秋 毛宝霖 +4 位作者 肖平 于维平 王应龙 黄常志 褶湘珍 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1993年第2期47-51,共5页
Se malt cakes containing 300μg selenium were taken up daily to men from high risk area in lung cancer and the influences of Ultraviolet (UV) and Benaopyrene (BαP) induced unscheduled DNA synthesis (UDS) were determi... Se malt cakes containing 300μg selenium were taken up daily to men from high risk area in lung cancer and the influences of Ultraviolet (UV) and Benaopyrene (BαP) induced unscheduled DNA synthesis (UDS) were determined. The Se levels in serum, hair and activity of GSH-px were increased by 89%, 67% and 178%, respectively, after Se-supplementation for half year. In the UV treatment, the ratio of UDS was decreased from the mean values of 2. 47 in the control to 1. 61 (P<0. 05) in the Se-group, In the BaP treatment, furthermore, the elevated Se levels of 78% in serum and 83% in the hair accompanied with 56% high in activity of GSH-px were followed by the Se intake for one year, while the mean value of UDS was reduced from 2. 21 in the control to 1. 47(P< 0. 05) in the group of selenium tested. The blocking effects of UV irradiation and BaP treatment induced UDS of peripheral lymphocytes were showed in the Se-supplementation. 展开更多
关键词 Unscheduled dna synthesis (UDS).Selenium ULTRAVIOLET Benzopyrene
下载PDF
上一页 1 2 28 下一页 到第
使用帮助 返回顶部