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Human-Machine Symbiosis:Philosophical Reflection on Virtual Human
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作者 TAO Feng 《Cultural and Religious Studies》 2024年第5期286-294,共9页
Virtual human is the simulation of human under the synthesis of virtual reality,artificial intelligence,and other technologies.Modern virtual human technology simulates both the external characteristics and the intern... Virtual human is the simulation of human under the synthesis of virtual reality,artificial intelligence,and other technologies.Modern virtual human technology simulates both the external characteristics and the internal emotions and personality of humans.The relationship between virtual human and human is a concrete expression of the modern symbiotic relationship between human and machine.This human-machine symbiosis can either be a fusion of the virtual human and the human or it can cause a split in the human itself. 展开更多
关键词 virtual human symbiosis SUSTAINABILITY MACHINE INDUSTRY
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An Efficient Approach for Transforming Unbalanced Transportation Problems into Balanced Problems in Order to Find Optimal Solutions
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作者 Abdur Rashid Md. Amirul Islam 《American Journal of Operations Research》 2024年第1期74-86,共13页
In operations research, the transportation problem (TP) is among the earliest and most effective applications of the linear programming problem. Unbalanced transportation problems reflect the reality of supply chain a... In operations research, the transportation problem (TP) is among the earliest and most effective applications of the linear programming problem. Unbalanced transportation problems reflect the reality of supply chain and logistics situations where the available supply of goods may not precisely match the demand at different locations. To deal with an unbalanced transportation problem (UTP), it is essential first to convert it into a balanced transportation problem (BTP) to find an initial basic feasible solution (IBFS) and hence the optimal solution. The present paper is concerned with introducing a new approach to convert an unbalanced transportation problem into a balanced one and as a consequence to obtain optimum total transportation cost. Numerical examples are provided to demonstrate the suggested method. 展开更多
关键词 unbalanced Transportation Problem (UTP) Supply DEMAND Initial Solution Optimal Solution
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Research on Geographical Environment and Culture Symbiosis of Chishui River Basin 被引量:1
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作者 王爱华 彭恩 《Journal of Landscape Research》 2011年第11期81-85,共5页
This paper took Chishui River Basin as the research object,and pointed out that its culture,which reflected local life and production and was featured with regional characteristics as well as abundant cultural connota... This paper took Chishui River Basin as the research object,and pointed out that its culture,which reflected local life and production and was featured with regional characteristics as well as abundant cultural connotation,was constituted by native natural and cultural geography,folk customs and historical feature.The relation between Chishui salt path and salt business culture under the typical closed and half-closed geographical environment was analyzed to indicate that the realignment of Chishui River had brought prosperity of Chishui River,booming of commercial towns,and development of ancient architectures and guild hall culture.The relation between rich natural resources and production and life culture of Chishui River was explained to show that Danxia landform nurtured stone culture,Chishui River resources cultivated fishing culture,special hydrogeological environment fostered liquor culture represented by Maotai,and Bamboo culture accumulated for hundreds of years.Finally,it introduced military culture and the spirit of the Long March forming based on the special location of Chishui River.It emphasized that geological environment was the important basis for human's survival and played an immeasurable role in the cultural development of a region. 展开更多
关键词 Chishui RIVER BASIN CULTURE GEOGRAPHICAL ENVIRONMENT symbiosis
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A Survey of Unbalanced Flow Diagnostics and Their Application 被引量:23
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作者 Fuqing Zhang Steven E. Koch Christopher A. Davis and Michael L. Kaplan 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2000年第2期165-183,共19页
This paper presents an extensive survey of the most commonly used tools for diagnosing unbalanced flow in the atmosphere, namely the Lagrangian Rossby number, Psi vector, divergence equation, nonlinear balance equatio... This paper presents an extensive survey of the most commonly used tools for diagnosing unbalanced flow in the atmosphere, namely the Lagrangian Rossby number, Psi vector, divergence equation, nonlinear balance equation, generalized omega-equation, and departure from fields obtained by potential vorticity (PV) inversion. The basic thoery, assumptions as well as implementation and limitations for each of the tools are all discussed. These tools are applied to high—resolution mesoscale model data to assess the role of unbalanced dynamics in the generation of a mesoscale gravity wave event over the East Coast of the United States. Comparison of these tools in this case study shows that these various methods agree to a large extent with each other though they differ in details. Key words Unbalanced flow - Geostrophic adjustment - Gravity waves - Nonlinear balance equation - Potential vorticity inversion - Omega equations - Rossby number This research was conducted under support from NSF grant ATM-9700626 of the United States. The numerical computations described herein were performed on the Cray T90 at the North Carolina Supercomputing Center and the Cray supercomputer at the NCAR Scientific Computing Division, which also provided the initialization fields for the MM5. Thanks are extended to Mark Stoelinga at University of Washington for the RIP post-processing package. 展开更多
关键词 unbalanced flow Geostrophic adjustment Gravity waves Nonlinear balance equation Potential vorticity inversion Omega equations Rossby number
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Influence and determinative factors of ion-to-atom arrival ratio in unbalanced magnetron sputtering systems 被引量:8
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作者 Jun Zhou Zhe Wu Zhanhe Liu 《Journal of University of Science and Technology Beijing》 CSCD 2008年第6期775-781,共7页
Low pressure sputtering with a controlled ratio of ion flux to deposited atom flux at the condensing surface is one of the main directions of development of magnetron sputtering methods. Unbalanced magnetron sputterin... Low pressure sputtering with a controlled ratio of ion flux to deposited atom flux at the condensing surface is one of the main directions of development of magnetron sputtering methods. Unbalanced magnetron sputtering, by producing dense secondary plasma around the substrate, provides a high ion current density. The closed-field unbalanced magnetron sputtering system (CFUBMS) has been established as a versatile technique for high-rate deposition high-quality metal, alloy, and ceramic thin films. The'key factor in the CFUBMS system is the ability to transport high ion currents to the substrate, which can enhance the formation of full dense coatings at relatively low value homologous temperature. The investigation shows that the energy of ions incidenced at the substrate and the ratio of the flux of these ions to the flux of condensing atoms are the fundamental parameters in determining the structure and properties of films produced by ion-assisted deposition processes. Increasing ion bombardment during deposition combined with increasing mobility of the condensing atoms favors the formation of a dense microstructure and a smooth surface. 展开更多
关键词 magnetron sputtering closed-field unbalanced magnetron sputtering system (CFUBMS) ion-to-atom ratio unbalancedmagnetron sputtering
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Symbiosis of Marshes and Permafrost in Da and Xiao Hinggan Mountains in Northeastern China 被引量:14
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作者 JIN Huijun SUN Guangyou +2 位作者 YU Shaopeng JIN Rui HE Ruixia 《Chinese Geographical Science》 SCIE CSCD 2008年第1期62-69,共8页
Recently, the degradation of permafrost and marsh environments in the Da and Xiao Hinggan Mountains has become a great concern as more human activities and pronounced climate warming were observed during the past 30 y... Recently, the degradation of permafrost and marsh environments in the Da and Xiao Hinggan Mountains has become a great concern as more human activities and pronounced climate warming were observed during the past 30 years and projected for the near future. The distr/bution patterns and development mechanisms of the permafrost and marshes have been examined both in theories and in field observations, in order to better understand the symbiosis of permafrost and marshes. The permafrost and marshes in the Da and Xiao Hinggan Mountains display discernible zonations in latitude and elevation. The marsh vegetation canopy, litter and peat soil have good thermal insulation properties for the underlying permafrost, resulting in a thermal offset of 3 ℃ to 4℃ and subsequently suppressing soil temperature. In addition, the much higher thermal conductivity of frozen and ice-rich peat in the active layer is conducive to the development or in favor of the protection of permafrost due to the semi-conductor properties of the soils overlying the permafrost. On the other hand, because permafrost is almost impervious, the osmosis of water in marsh soils can be effectively reduced, timely providing water supplies for helophytes growth or germination in spring. In the Da and Xiao Hinggan Mountains, the permafrost degradation has been accelerating due to the marked climate warming, ever increasing human activities, and the resultant eco-environmental changes. Since the permafrost and marsh environments are symbiotic and interdependent, they need to be managed or protected in a well-coordinated and integrated way. 展开更多
关键词 marsh PERMAFROST symbiosis thermal offset active layer Da and Xiao Hinggan Mountains
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LINEAR PROVABLE SECURITY FOR A CLASS OF UNBALANCED FEISTEL NETWORK 被引量:3
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作者 Wang Nianping Jin Chenhui Yu Zhaoping 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2005年第4期401-406,共6页
A structure iterated by the unbalanced Feistel networks is introduced. It is showed that this structure is provable resistant against linear attack. The main result of this paper is that the upper bound of r-round (r... A structure iterated by the unbalanced Feistel networks is introduced. It is showed that this structure is provable resistant against linear attack. The main result of this paper is that the upper bound of r-round (r≥2m) linear hull probabilities are bounded by q^2 when around function F is bijective and the maximal linear hull probabilities of round function F is q. Application of this structure to block cipher designs brings out the provable security against linear attack with the upper bounds of probabilities. 展开更多
关键词 unbalanced Feistel networks provable security against linear attack linear hull probabilities upper bound.
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Modeling and optimization of unbalanced multi-stage logistic system
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作者 徐杭 徐榕 叶庆泰 《Journal of Southeast University(English Edition)》 EI CAS 2005年第2期220-224,共5页
To decompose an unbalanced multi-stage logistic system to multipleindependent single-stage logistic systems, a new notion of parameterized interface distribution ispresented. For encoding the logistic pattern on each ... To decompose an unbalanced multi-stage logistic system to multipleindependent single-stage logistic systems, a new notion of parameterized interface distribution ispresented. For encoding the logistic pattern on each stage, the Pruefer number is used. With theimproved decoding procedure, any Pruefer number produced stochastically can be decoded to a feasiblelogistic pattern, which can match with the capacities of the nodes of the logistic system. Withthese two innovations, a new modeling method based on parameterized interface distribution and thePriifer number coding is put forward. The corresponding genetic algorithm, named as PIP-GA, can findbetter solutions and require less computational time than st-GA. Although requiring a little moreconsumption of memory, PIP-GA is still an efficient and robust method in the modeling andoptimization of unbalanced multi-stage logistic systems. 展开更多
关键词 LOGISTICS optimization genetic algorithm Priifer number spanning tree parameterized interface distribution unbalanced multi-stage logistic system
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Torsional vibration analysis of lathe spindle system with unbalanced workpiece 被引量:5
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作者 郭瑞 JANG Sung-Hyun CHOI Young-Hyu 《Journal of Central South University》 SCIE EI CAS 2011年第1期171-176,共6页
For the purpose of analyzing the torsional vibration caused by the gravitational unbalance torque arisen in a spindle system when it is machining heavy work piece,a 10-DOF lumped parameter model was made for the machi... For the purpose of analyzing the torsional vibration caused by the gravitational unbalance torque arisen in a spindle system when it is machining heavy work piece,a 10-DOF lumped parameter model was made for the machine tool spindle system with geared transmission.By using the elementary method and Runge-Kutta method in Matlab,the eigenvalue problem was solved and the pure torsional vibration responses were obtained and examined.The results show that the spindle system cannot operate in the desired constant rotating speed as far as the gravitational unbalance torque is engaged,so it may cause bad effect on machining accuracy.And the torsional vibration increases infinitely near the resonant frequencies,so the spindle system cannot operate normally during these spindle speed ranges. 展开更多
关键词 unbalanced work piece gravitational unbalance torque torsional vibration spindle system geared transmission
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The Effect of Unbalanced Coefficient of Magnetron on the Structure and Properties of CN_x Coatings 被引量:1
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作者 文晓斌 李显 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2011年第2期274-280,共7页
The effect of unbalanced coefficient of magnetron (UCM) on the structure and tribological properties of CrNx hard coatings was studied.The CrNx coatings were deposited on both Si wafer and hardened tool steel substr... The effect of unbalanced coefficient of magnetron (UCM) on the structure and tribological properties of CrNx hard coatings was studied.The CrNx coatings were deposited on both Si wafer and hardened tool steel substrates using a closed-field unbalanced magnetron sputtering ion plating technique in a gas mixture of Ar+N2 under different unbalanced magnetron conditions.The coatings were characterized by means of XRD,XPS,SEM,microhardness tester and pin-on-disc tribometer to study respectively their structure,chemical bonding state,microstructure,hardness and tribological properties.The experimental results show that the UCM has a profound effect on the structure,hardness and tribological properties of the CrNx coatings.With increasing the values of UCM,the dominant phases in the deposited coatings evolved from Cr+Cr2N to Cr2N+CrN,the microstructure became denser and the hardness increased;in addition,reduced coefficient of friction and improved wear resistance of CrNx coatings were also observed under a larger UCM. 展开更多
关键词 magnetron sputtering unbalanced coefficient CrNx coating structure properties
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A Nonlinear Control Strategy for Vienna Rectifier Under Unbalanced Input Voltage 被引量:1
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作者 REN Xiaoyong CHEN Yu +2 位作者 TONG Dan ZHANG Zhiliang CHEN Qianhong 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2019年第5期703-714,共12页
The Vienna rectifier with unbalanced input voltage and load transient is analyzed.A nonlinear control strategy for Vienna rectifier under unbalanced input is proposed.From the view of positive and negative sequence co... The Vienna rectifier with unbalanced input voltage and load transient is analyzed.A nonlinear control strategy for Vienna rectifier under unbalanced input is proposed.From the view of positive and negative sequence components,the proposed nonlinear control strategy suppresses the twice frequency ripple and guarantees the dynamic response characteristic at the same time.Thanks to the proposed nonlinear control strategy,the DC bus capacitor can be reduced a lot since the voltage ripple and drop can be suppressed.A 10 kW Vienna rectifier is built to verify the proposed control strategy.After applying the proposed nonlinear control strategy,the voltage ripple is only7 V and decreases over 75%over the traditional PI control when the unbalanced degree is 20%.The voltage drop can be reduced about 80%than former control strategy which is helpful to reduce the DC bus capacitor and achieve higher power density.The volume of the capacitor can be reduced by 83.3%with the new control method. 展开更多
关键词 VIENNA RECTIFIER radars unbalanced INPUT voltage NONLINEAR control strategy DC BUS CAPACITANCE
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Cu Films Deposited by Unbalanced Magnetron Sputtering Enhanced by ICP and External Magnetic Field Confinement 被引量:1
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作者 齐雪莲 任春生 +1 位作者 马腾才 王友年 《Plasma Science and Technology》 SCIE EI CAS CSCD 2008年第3期319-322,共4页
Metallic copper(Cu) films were deposited on a Si (100) substrate by unbalanced magnetron sputtering enhanced by radio-frequency plasma and external magnetic field confinement. The morphology and structure of the f... Metallic copper(Cu) films were deposited on a Si (100) substrate by unbalanced magnetron sputtering enhanced by radio-frequency plasma and external magnetic field confinement. The morphology and structure of the films were examined by scanning electron microscopy (SEM), atomic force microscope (AFM) and X-ray diffraction (XRD). The surface average roughness of the deposited Cu films was characterized by AFM data and resistivity was measured by a four-point probe. The results show that the Cu films deposited with radio-frequency discharge enhanced ionization and external magnetic field confinement have a smooth surface, low surface roughness and low resistivity. The reasons may be that the radio-frequency discharge and external magnetic field enhance the plasma density, which further improves the ion bombardment effect under the same bias voltage conditions. Ion bombardment can obviously influence the growth features and characteristics of the deposited Cu films. 展开更多
关键词 unbalanced magnetron sputtering RADIO-FREQUENCY magnetic field Cu film
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Gyroscope Fault Diagnosis Using Fuzzy SVM to Unbalanced Samples 被引量:1
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作者 罗秋凤 张锐 +1 位作者 李勇 杨忠清 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI CSCD 2015年第1期16-21,共6页
A novel fuzzy support vector machine based on unbalanced samples(FSVM-US)is proposed to solve the high false positive rate problem since the gyroscope output is susceptible to unmanned aerial vehicle(UAV)airborne elec... A novel fuzzy support vector machine based on unbalanced samples(FSVM-US)is proposed to solve the high false positive rate problem since the gyroscope output is susceptible to unmanned aerial vehicle(UAV)airborne electromagnetic environment and the gyroscope abnormal signal sample is rather rare.Firstly,the standard deviation of samples projection to normal vector for SVM classifier hyper plane is analyzed.The imbalance feature expression reflecting the hyper plane shift for the number imbalance between samples and the dispersion imbalance within samples is derived.At the same time,the denoising factor is designed as the exponential decay function based on the Euclidean distance between each sample and the class center.Secondly,the imbalance feature expression and denoising factor are configured into the membership function.Each sample has its own weight denoted the importance to the classifier.Finally,the classification simulation experiments on the gyroscope fault diagnosis system are conducted and FSVM-US is compared with the standard SVM,FSVM,and the four typical class imbalance learning(CIL)methods.The results show that FSVM-US classifier accuracy is 12% higher than that of the standard SVM.Generally,FSVM-US is superior to the four CIL methods in total performance.Moreover,the FSVMUS noise tolerance is also 17% higher than that of the standard SVM. 展开更多
关键词 fault diagnosis GYROSCOPE fuzzy support vector machine(FSVM) unbalanced samples membership function
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Enhanced control of DFIG-used back-to-back PWM VSC under unbalanced grid voltage conditions 被引量:20
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作者 HU Jia-bing HE Yi-kang NIAN Heng 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2007年第8期1330-1339,共10页
This paper presents a unified positive-and negative-sequence dual-dq dynamic model of wind-turbine driven doubly-fed induction generator(DFIG) under unbalanced grid voltage conditions. Strategies for enhanced control ... This paper presents a unified positive-and negative-sequence dual-dq dynamic model of wind-turbine driven doubly-fed induction generator(DFIG) under unbalanced grid voltage conditions. Strategies for enhanced control and operation of a DFIG-used back-to-back(BTB) PWM voltage source converter(VSC) are proposed. The modified control design for the grid-side converter in the stationary αβ frames diminishes the amplitude of DC-link voltage ripples of twice the grid frequency,and the two proposed control targets for the rotor-side converter are alternatively achieved,which,as a result,improve the fault-ride through(FRT) capability of the DFIG based wind power generation systems during unbalanced network supply. A complete unbalanced control scheme with both grid-and rotor-side converters included is designed. Finally,simulation was carried out on a 1.5 MW wind-turbine driven DFIG system and the validity of the developed unified model and the feasibility of the proposed control strategies are all confirmed by the simulated results. 展开更多
关键词 unbalanced grid voltage Doubly-fed induction generator (DFIG) Back-to-back (BTB) Fault-ride through (FRT) Voltage source converter (VSC)
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Arbuscular mycorrhizal symbiosis and ecosystem processes: Prospects for future research in tropical soils 被引量:1
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作者 Geofrey Soka Mark Ritchie 《Open Journal of Ecology》 2014年第1期11-22,共12页
Arbuscular mycorrhizal fungi (AMF) are more widely distributed and can associate with a wide range of plant species. AMF are keystone organisms that form an interface between soils and plant roots. They are also sensi... Arbuscular mycorrhizal fungi (AMF) are more widely distributed and can associate with a wide range of plant species. AMF are keystone organisms that form an interface between soils and plant roots. They are also sensitive to environmental changes. AMF are important microbial symbioses for plants under conditions of P-limitation. The AMF are crucial for the functioning of terrestrial ecosystems as they form symbiotic interactions with plants. Mycorrhizal fungi are known to influence plant diversity patterns in a variety of ecosystems globally. AMF hyphae form an extensive network in the soil. The length is a common parameter used to quantifying fungal hyphae. The mycelial network of AM fungi extends into the soil volume and greatly increases the surface area for the uptake of immobile nutrients. Also, AM symbioses improve plants tolerance to drought and enhance plants’ tolerance of or resistance to root pathogens. Also, the networks of AM hyphae play a crucial role in the formation of stable soil aggregates and in the building up of a macroporous structure of soil that allows penetration of water and air and thereby prevents erosion. The functioning of AMF symbiosis is mediated by direct and indirect effects of biotic and abiotic factors of the surrounding rhizosphere, the community, and the ecosystem. AMF have great potential in the restoration of disturbed land and low fertility soil. However, despite the importance of AMF to terrestrial ecosystems, little is known about the effects of environmental changes on AMF abundance, activity and the impact of these changes on the ecosystem services. Therefore, it is important to gain a clearer understanding of the effects of environmental changes on the AM fungal species to guide conservation and restoration efforts. 展开更多
关键词 ARBUSCULAR MYCORRHIZAL symbiosis HYPHAE Soil TROPICAL ECOSYSTEM
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Study on Acid Tolerance in Rhizobium meliloti and Its Symbiosis System 被引量:1
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作者 Jiang Hua Duan Xinhui +1 位作者 Shan Guilian He Chenggang 《Animal Husbandry and Feed Science》 CAS 2014年第3期104-106,112,共4页
Soil pH is a main factor that affects the nodule formation and nitrogen fixation. The largely reduced nitrogen fixation effect caused by acid soil is the ma- jor limitation of alfalfa growth in the south of China. Thu... Soil pH is a main factor that affects the nodule formation and nitrogen fixation. The largely reduced nitrogen fixation effect caused by acid soil is the ma- jor limitation of alfalfa growth in the south of China. Thus selection of anti-acid nodule for inoculating on alfalfa is of great significance in practical production. Using nine candidate rhizobia ( Rhizobium meliloti L. ) as study objects, their colony diameters and the nodule number of each strain inoculated alfalfa variety were investigated at different pH conditions. The YNCY006 strain presented the strongest resistance to acid, while YNCY007 strain presented the weakest. For nedulation, GT13R alfalfa inoculated with YNCY006 or YNCY008 strains was the best symbiosis system in the acidic soil. 展开更多
关键词 ALFALFA Rhizobium meliloti L. Acid tolerance symbiosis system
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Symbiosis Analysis on Industrial Ecological System 被引量:1
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作者 汪刚 冯宵 Khim Hoong Chu 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2014年第6期690-698,共9页
Industrial ecological system is a sustainable mode of modern industry development. Industrial symbiosis, a sub-field of industrial ecology, engages traditionally separate industries in a collective approach, involving... Industrial ecological system is a sustainable mode of modern industry development. Industrial symbiosis, a sub-field of industrial ecology, engages traditionally separate industries in a collective approach, involving exchange of materials, energy, water, and/or by-products, to enhance competitive ability and environmental performance. To construct a symbiosis analysis method, this article employs a number of parameters embodying information about materials, energy and economics as the main essential parameters in system analysis and introduces symbiosis profit and symbiotic consumption elements as the economic indicators. A modeling and simulation program is designed using the agent-based modeling approach to simulate the evolvement of a hypothetical coal-based industrial system and the change of symbiosis conditions in the process of construction is examined. The simulation program built using the Swarm library, which is a freely available multi-agent simulation package, provides a useful demonstration for the symbiosis analysis method. 展开更多
关键词 symbiosis analysis symbiosis degree agent-based modeling multi-agent simulation coal-based industrial system
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Fabrication of Diamond-like Carbon Films by Ion Assisted Middle Frequency Unbalanced Magnetron Sputtering 被引量:1
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作者 ZHANG Yi-chen SUN Shao-ni +2 位作者 ZHOU Yi MA Sheng-ge BA De-chun 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2006年第B12期119-125,共7页
Diamond-like carbon (DLC) films are deposited by the Hall ion source assisted by the mid-frequency unbalanced magnetron sputtering technique. The effects of the substrate voltage bias, the substrate temperature, the... Diamond-like carbon (DLC) films are deposited by the Hall ion source assisted by the mid-frequency unbalanced magnetron sputtering technique. The effects of the substrate voltage bias, the substrate temperature, the Hall discharging current and the argon/nitrogen ratio on the DLC film's performance were studied. The experimental results show that the film's surface roughness, the hardness and the Young's modulus increase firstly and then decrease with the bias voltage incrementally increases. Also when the substrate temperature rises, the surface roughness of the film varies slightly, but its hardness and Young's modulus firstly increase followed by a sharp decrease when the temperature surpassing 120 ℃. With the Hall discharging current incrementally rising, the hardness and Young's modulus of the film decrease and the surface roughness of the film on 316L stainless steel firstly decreased and then remains constant. 展开更多
关键词 fluid machinery and engineering diamond-like carbon films middle frequency unbalanced magnetron sputtering hall ion source
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The full length <i>PtSRP</i>(<i>Pisolithus tinctorius</i>symbiosis related protein) fungal mRNA encodes a potential marker of ectomycorrhiza formation 被引量:1
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作者 Helder Elísio E. Vieira Cláudia E. P. Lima +2 位作者 Carlos E. Calzavara-Silva Bartolomeu Acioli-Santos Elaine Malosso 《American Journal of Molecular Biology》 2012年第3期258-264,共7页
The Pisolithus tinctorius symbiosis related protein expressed sequence tag (EST PtSRP) was previously identified in the first hours of the interaction between the fungus Pisolithus tinctorius and sweet chestnut Castan... The Pisolithus tinctorius symbiosis related protein expressed sequence tag (EST PtSRP) was previously identified in the first hours of the interaction between the fungus Pisolithus tinctorius and sweet chestnut Castanea sativa, and partially characterized as a fungal marker gene of ectomycorrhizal symbiosis formation. We used the 5’ rapid amplification of cDNA ends (RACE) to obtain the PtSRP mRNA 5’ region, and together with our previously reported 3’ mRNA region, the full mRNA sequence was assembled by use of bioinformatics tools and deposited to GenBank (Accession: GU733439). The full-length mRNA sequence (636 bp) revealed the locations of the 5’ and 3’ untranslated regions (UTRs) and contained the Kozak sequence (ccc aag ATG A) in the 5’ UTR. The in silico translated PtSRP open reading frame (ORF) codes for a 127 amino acid protein and contained four putative post-translational modification sites (two N-glycosylation and two phosphorylation). The protein secondary structure is postulated to be composed of one N-terminal hydrophobic transmembrane alpha helix and at least six hydrophilic beta-strands spread across the protein. Sub-cellular localization prediction suggests that the protein is involved in cellular secretory pathway, supported by the presence of a cleavage site motif close to the membrane anchor. The data presented herein indicate the role of PtSRP as a fungal membrane secreted protein involved in early stages of ectomycorrhizal formation, with application as a possible marker for nascent ectomy-corrhiza fungal development. 展开更多
关键词 ECTOMYCORRHIZA symbiosis RELATED Genes/Proteins RACE EST
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Arbuscular mycorrhizal symbiosis facilitates apricot seedling(Prunus sibirica L.)growth and photosynthesis in northwest China 被引量:1
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作者 Yinli Bi Linlin Xie +3 位作者 Zhigang Wang Kun Wang Wenwen Liu Wenwu Xie 《International Journal of Coal Science & Technology》 EI CAS CSCD 2021年第4期473-482,共10页
Arbuscular mycorrhizal(AM)fungi can successfully enhance photosynthesis(P_(n))and plants growth in agricultural or grassland ecosystems.However,how the symbionts affect species restoration in sunlight-intensive areas ... Arbuscular mycorrhizal(AM)fungi can successfully enhance photosynthesis(P_(n))and plants growth in agricultural or grassland ecosystems.However,how the symbionts affect species restoration in sunlight-intensive areas remains largely unexplored.Therefore,this study’s objective was to assess the effect of AM fungi on apricot seedling physiology,within a specific time period,in northwest China.In 2010,an experimental field was established in Shaanxi Province,northwest China.The experimental treatments included two AM fungi inoculation levels(0 or 100 g of AM fungal inoculum per seedling),three shade levels(1900,1100,and 550µmol m^(−2) s^(−1)),and three ages(1,3,and 5 years)of transplantation.We examined growth,Pn,and morphological indicators of apricot(Prunus sibirica L.)seedling performances in 2011,2013,and 2015.The colonization rate in mycorrhizal seedlings with similar amounts of shade is higher than the corresponding controls.The mycorrhizal seedling biomass is significantly higher than the corresponding non-mycorrhizal seedling biomass.Generally,P_(n),stomatal conductance(G_(s)),transpiration rate(T_(r)),and water use efficiency are also significantly higher in the mycorrhizal seedlings.Moreover,mycorrhizal seedlings with light shade(LS)have the highest Pn.WUE is increased in non-mycorrhizal seedlings because of the reduction in T_(r),while T_(r) is increased in mycorrhizal seedlings with shade.There is a significant increase in the N,P,and K fractions detected in roots compared with shoots.This means that LS had apparent benefits for mycorrhizal seedlings.Our results also indicate that AM fungi,combined with LS,exert a positive effect on apricot behavior. 展开更多
关键词 Apricot seedlings Arbuscular mycorrhizal symbiosis Plant growth Light shade Photosynthesis
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