GNSS spoofing detection based on uncultivated wolf pack algorithm

In order to solve the problem that the global navigation satellite system（GNSS） receivers can hardly detect the GNSS spoofing when they are deceived by a spoofer,a model-based approach for the identification of the GNSS spoofing is proposed.First,a Hammerstein model is applied to model the spoofer/GNSS transmitter and the wireless channel.Then,a novel method based on the uncultivated wolf pack algorithm（UWPA） is proposed to estimate the model parameters.Taking the estimated model parameters as a feature vector,the identification of the spoofing is realized by comparing the Euclidean distance between the feature vectors.Simulations verify the effectiveness and the robustness of the proposed method.The results show that,compared with the other identification algorithms,such as least square（LS）,the iterative method and the bat-inspired algorithm（BA）,although the UWPA has a little more time-eomplexity than the LS and the BA algorithm,it has better estimation precision of the model parameters and higher identification rate of the GNSS spoofing,even for relative low signal-to-noise ratios.

Identification of ^(137)Cs Reference Sites in Southeastern China

The 137Cs vertical distributions in uncultivated and cultivated soils, developed from Quaternary red clay, granite, argillaceous shale, and red sandstone, were studied to develop reliable guidelines for selecting reference sites in southeastern China, which is dominated by strong acidic and/or clay-textured soils, and examine their reliability by comparing them to the reported 137Cs reference inventory data to see whether they agreed with the global distribution pattern. It was observed that a relatively high proportion of 137Cs was concentrated in the surface layers of soils with relatively high clay content. In the paddy soils developed from granite more 137Cs penetrated to depths below the plow layer (about 45.3%), when compared to those from the other three parent materials. The relatively low soil 137Cs inventories on crests excluded using the crest as the 137Cs reference site; instead the paddy field on the hillock plain was selected. Furthermore, within a specific county characterized by great systematic spatial variations of rainfall and topography across the landscape, a significant (P<0.01) and positive linear relationship (r2=0.81) between local 137Cs inventory and corresponding local annual rainfall was observed. Thus, for areas with large variations in rainfall, a single uniform value of local 137Cs reference inventory should be used with caution.

^(137)Cs and^(210)Pb_(ex) as Soil Erosion Tracers in the Hilly Sichuan Basin and the Three Gorges Area of China

Accelerated soil erosion and land degradation represent major environmental problems for agricultural lands.Reliable information on the rates of soil loss is urgently needed.The traditional techniques for documenting rates of soil loss may meet this need,but face many limitations.The fallout radionuclides,especially 137 Cs and 210 Pb ex,are increasingly used as effective tracers to quantify soil erosion rates,and they represent a valuable complement to the existing classical methods.This paper aims to introduce the basis for assessing soil erosion rates on cultivated and uncultivated slopes by using 137 Cs and 210 Pb ex measurements,to compare the 137 Cs and 210 Pb ex reference inventories,and to report several case studies undertaken in the hilly area of Sichuan Basin and the Three Gorges area of China.

Towards enriching and isolation of uncultivated archaea from marine sediments using a refined combination of conventional microbial cultivation methods

The archaea that can be readily cultivated in the laboratory are only a small fraction of the total diversity that exists in nature.Although molecular ecology methods,such as metagenomic sequencing,can provide valuable information independent of cell cultivation,it is only through cultivation-based experiments that they may be fully characterized,both for their physiological and ecological properties.Here,we report our efforts towards enriching and isolation of uncultivated archaea from marine sediments using a refined combination of conventional microbial cultivation methods.Initially,cells were retrieved from the sediment samples through a cell extraction procedure and the sediment-free mixed cells were then divided into different size-range fractions by successive filtration through 0.8µm,0.6µm and 0.2µm membranes.Archaeal 16S rRNA gene analyses indicated noticeable retention of different archaeal groups in different fractions.For each fraction,supplementation with a variety of defined substrates(e.g.,methane,sulfate,and lignin)and stepwise dilutions led to highly active enrichment cultures of several archaeal groups with Bathyarchaeota most prominently enriched.Finally,using a roll-bottle technique,three co-cultures consisting of Bathyarchaeota(subgroup-8)and a bacterial species affiliated with either Pseudomonas or Glutamicibacter were obtained.Our results demonstrate that a combination of cell extraction,size fractionation,and roll-bottle isolation methods could be a useful protocol for the successful enrichment and isolation of numerous slow-growing archaeal groups from marine sediments.

Elevated and atmospheric-level methane consumption by soil methanotrophs of three grasslands in China

Background:Methane(CH4)oxidation driven by soil aerobic methanotrophs demonstrates the capacity of grassland as a CH4 sink.Methods:In this study,we compared the oxidation characteristics of atmospheric-level and elevated concentration(10%)CH4 in a typical grassland(steppe)on the Loess Plateau,an alpine meadow(meadow)on the Qinghai-Tibet Plateau,and an inland arid-area artificial grassland(pasture)in northwest China and investigated the communities of active methanotrophs and their contribution to CH4 oxidation using DNA-based stable-isotope probing and Illumina Miseq sequencing.Results:The results showed that the oxidation of atmospheric CH4 only occurred in steppe and meadow soils where the USCγgroup of methanotrophs was numerically dominant in the methanotroph community.Pasture soils,with their very low relative abundance of USCγ,did not show atmospheric CH4 oxidation.However,a DNA-stable isotope probing experiment with 10%CH4 indicated that conventional CH4 oxidizers(Methylocaldum and Methylocystis)rather than USCγcommunities assimilated significant amounts of 13CH4 for growth.Conclusions:The CH4 oxidation mechanisms in the three experimental grassland soils varied significantly.The USCγgroup may be obligate oligotrophic microorganisms or their growth requires specific unknown conditions.