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EXPLANT AXENISATION FOR TISSUE CULTURE IN MARINE MACROALGAE 被引量:1
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作者 刘学武 Bernard Kloareg 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 1992年第3期268-277,共10页
Unialgal explants from Luminaria digitata, and from a variety of red algae were obtained by hand removing the visible epiphytes, and stirring the tissue in the presence of glass beads. Two antibiotic mixtures were fou... Unialgal explants from Luminaria digitata, and from a variety of red algae were obtained by hand removing the visible epiphytes, and stirring the tissue in the presence of glass beads. Two antibiotic mixtures were found to be efficient in removing the contaminating fungi and bacteria from the algae. The procedure proved suitable as a primary step in the tissue culture of the investigated species. 展开更多
关键词 unialgai. a.xenic. antibiotics disinfectants callus regeneration
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