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Low Co-Cultivation Temperature at 20°C Resulted in the Reproducible Maximum Increase in Both the Fresh Weight Yield and Stable Expression of GUS Activity after <i>Agrobacterium tumefaciens</i>-Mediated Transformation of Tobacco Leaf Disks 被引量:3
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作者 Guiying Su Sunjung Park +1 位作者 Seokhyun Lee Norimoto Murai 《American Journal of Plant Sciences》 2012年第4期537-545,共9页
The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Ni... The importance of controlled temperature during the four-days co-cultivation period was evaluated under the most physiologically relevant conditions for Agrobacterium tumefaciens-mediated transformation of tobacco (Nicotiana tabacum L. cv. Xanthi (nn, Smith)) leaf disks. We compared the effect of temperatures ranging from 15°C, 18°C, 20°C, 22°C to 25°C on the stable expression of β-glucuronidase (GUS) activity of 14 days old hygromycin-selected leaf disks, and on the increase in the fresh weight yield of 28 days old kanamycin-selected calli. The highest average of GUS activity was obtained at 20°C among the five temperatures tested although the difference between the 18°C and 20°C treatment was not statistically significant. The GUS activity at 15°C was statistically lower than those at 18°C and 20°C. The GUS activity in 22°C treatment was an intermediate between the highest (18/20°C) and second highest averages (15°C), and was not statistically significantly different. The lowest average of GUS activity was observed at 25°C. The highest increase in the plate average of fresh weight yield was obtained at 20°C among the five temperature tested. The 20°C treatment was statistically significantly better than the 15°C and 18°C treatments. The 20°C co-cultivation treatment resulted in the higher FW yield than 22°C and 25°C even though the differences were not statistically significant. In conclusion, low co-cultivation temperature at 20°C resulted in the reproducible maximum increase in both the fresh weight yield and stable expression of GUS activity after transformation of tobacco leaf disks. 展开更多
关键词 Agrobacterium TUMEFACIENS co-cultivation TEMPERATURE Fresh Weight Yield Stable GUS Gene Expression Tobacco Leaf DISKS Transformation
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Training System of Innovative and Entrepreneurial Talents based on University-Enterprise Collaboration
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作者 Zhang Jie Li Zijiang 《教育研究前沿(中英文版)》 2019年第3期122-126,共5页
With the continuous development of China's education,the diversification of personnel training has gradually become an important part of China's social development and construction.As a new teaching mode in hi... With the continuous development of China's education,the diversification of personnel training has gradually become an important part of China's social development and construction.As a new teaching mode in higher education,the university-enterprise collaborative training mode is an important guarantee for training high-quality,compound and practical professionals.Based on this,first of all,the current situation and problems of the training mode of innovative and entrepreneurial talents based on university-enterprise collaboration and its causes were expounded;then a new innovative and entrepreneurial talents training system based on university-enterprise collaboration was constructed from four aspects of education,practice,training and guidance;finally,the guarantee mechanism of the system was proposed from the aspects of culture,system and law,so as to promote the development of education in China. 展开更多
关键词 university-enterprise Collaboration Innovative and Entrepreneurial Talents Personnel Training Education Reform
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Integration of University-Enterprise in Applied Universities
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作者 Jingping Cao Jiancai Wang 《国际计算机前沿大会会议论文集》 2019年第2期428-429,共2页
Because higher education in China attaches more importance to theory rather than to practice, there is a gap between academic research and technical practice. With transformation and development of universities, to cu... Because higher education in China attaches more importance to theory rather than to practice, there is a gap between academic research and technical practice. With transformation and development of universities, to cultivate a large number of technical talents who can make scientific and technological achievements into practice, an educating strategy of integrating enterprises into higher education is proposed. From universities’ perspective, the cooperation with enterprises was further strengthened by linking the Industry- University-Research cooperation. With university-enterprise cooperation to indepth integration, the talent supply was further improved. And an in-depth ecological mechanism of government-led, market-driven and common development between universities and enterprises was established. The research result show that with the proposed strategy “win-win” cooperation between universities and enterprises is achieved. 展开更多
关键词 university-enterprise INTEGRATION ECOLOGICAL mechanism
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Research on the Teaching Reform of Radio-Frequency Identification Course Based on the Educational Philosophy of Outcome-Based Education
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作者 Dewen Cao 《Journal of Contemporary Educational Research》 2023年第4期7-11,共5页
In order to improve the teaching effect and quality of the course Radio-Frequency Identification,we investigated the characteristics of the course and problems in its teaching practice.Based on the educational philoso... In order to improve the teaching effect and quality of the course Radio-Frequency Identification,we investigated the characteristics of the course and problems in its teaching practice.Based on the educational philosophy of outcome-based education(OBE),we carried out teaching reforms,which included the use of flipped classroom,the organic combination of theoretical and practical teaching,school-enterprise cooperation and collaborative education,as well as other measures.The results showed that the improved teaching method can effectively stimulate students’interest in learning,enhance students’learning initiative,improve students’ability to solve complex engineering problems in the field of radio-frequency identification,cultivate the spirit of teamwork among students,and nurture their innovative practical ability. 展开更多
关键词 OBE RFID courses Accreditation of Engineering Education university-enterprise integration Online publication:April 25 2023
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Enhanced methane production in an anaerobic digestion and microbial electrolysis cell coupled system with co-cultivation of Geobacter and Methanosarcina 被引量:9
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作者 Qi Yin Xiaoyu Zhu +6 位作者 Guoqiang Zhan Tao Bo Yanfei Yang Yong Tao Xiaohong He Daping Li Zhiying Yan 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2016年第4期210-214,共5页
The anaerobic digestion(AD)and microbial electrolysis cell(MEC)coupled system has been proved to be a promising process for biomethane production.In this paper,it was found that by co-cultivating Geobacter with Me... The anaerobic digestion(AD)and microbial electrolysis cell(MEC)coupled system has been proved to be a promising process for biomethane production.In this paper,it was found that by co-cultivating Geobacter with Methanosarcina in an AD–MEC coupled system,methane yield was further increased by 24.1%,achieving to 360.2 m L/g-COD,which was comparable to the theoretical methane yield of an anaerobic digester.With the presence of Geobacter,the maximum chemical oxygen demand(COD)removal rate(216.8 mg COD/(L·hr))and current density(304.3 A/m3)were both increased by 1.3 and 1.8 fold compared to the previous study without Geobacter,resulting in overall energy efficiency reaching up to 74.6%.Community analysis demonstrated that Geobacter and Methanosarcina could coexist together in the biofilm,and the electrochemical activities of both were confirmed by cyclic voltammetry.Our study observed that the carbon dioxide content in total gas generated from the AD reactor with Geobacter was only half of that generated from the same reactor without Geobacter,suggesting that Methanosarcina may obtain the electron transferred from Geobacter for the reduction of carbon dioxide to methane.Taken together,Geobacter not only can improve the performance of the MEC system,but also can enhance methane production. 展开更多
关键词 AD–MEC coupled system Geobacter Methanosarcina co-cultivation Methane production
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Potential one-step strategy for PET degradation and PHB biosynthesis through co-cultivation of two engineered microorganisms 被引量:8
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作者 Pan Liu Tong Zhang +3 位作者 Yi Zheng Qingbin Li Tianyuan Su Qingsheng Qi 《Engineering Microbiology》 2021年第1期3-10,共8页
The management and recycling of plastic waste is a challenging global issue.Polyethylene terephthalate(PET),one of the most widely used synthetic plastics,can be hydrolyzed by a series of enzymes.However,upcycling the... The management and recycling of plastic waste is a challenging global issue.Polyethylene terephthalate(PET),one of the most widely used synthetic plastics,can be hydrolyzed by a series of enzymes.However,upcycling the resulting monomers is also a problem.In this study,we designed a co-cultivation system,in which PET degradation was coupled with polyhydroxybutyrate(PHB)production.First,PETase from Ideonalla sakaiensis was expressed in Yarrowia lipolytica Po1f with a signal peptide from lipase.The engineered PETase-producing Y.lipolytica was confirmed to hydrolyze bis(2-hydroxyethyl)terephthalate(BHET)and PET powder into the monomers terephthalate(TPA)and ethylene glycol(EG).Simultaneously,a TPA-degrading Pseudomonas stutzeri strain isolated from PET waste was transformed with a recombinant plasmid containing the phb CAB operon from Ralstonia eutropha,which encodes enzymes for the biosynthesis of PHB.The two co-cultivated engineered microbes could directly hydrolyze BHET to produce the bioplastic PHB in one fermentation step.During this process,5.16 g/L BHET was hydrolyzed in 12 h,and 3.66 wt%PHB(3.54 g/L cell dry weight)accumulated in 54 h.A total of 0.31g/L TPA was produced from the hydrolyzation of PET in 228 h.Although PHB could not be synthesized directly from PET because of the low hydrolyzing efficiency of PETase,this study provides a new strategy for the biodegradation and upcycling of PET waste by artificial microflora. 展开更多
关键词 Polyethylene terephthalate Upcycling POLYHYDROXYBUTYRATE PETase co-cultivation Yarrowia lipolytica Pseudomonas stutzeri
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School-Enterprise Cooperation in Excellent Engineer Education and Training Program at UESTC 被引量:1
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作者 Ting Zhong Guobin Zhu 《计算机教育》 2018年第12期138-140,共3页
School of Information and Software Engineering at University of Electronic Science and Technology of China(abbreviated hereafter as "SISE") joined Excellent Engineer Education and Training Program launched b... School of Information and Software Engineering at University of Electronic Science and Technology of China(abbreviated hereafter as "SISE") joined Excellent Engineer Education and Training Program launched by Ministry of Education of China in 2011. This paper discussed nine models of school-enterprise cooperation practiced at SISE since 2011. The school-enterprise cooperation model effectively broadens the students' horizons and improves the students' practical ability and has been recognized by academic circles. 展开更多
关键词 school-enterprise COOPERATION co-cultivation ENGINEER Education and TRAINING
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面向市场需求 加强企业合作——浅谈软件学院的企业合作工作(英文)
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作者 陈洑 徐谡 卢如海 《中山大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第S2期86-89,共4页
By exploring the reasons leading to problems in university-enterprise cooperation,the paper explains the collaboration necessity in talents training between demonstrative software school and enterprises,puts forward c... By exploring the reasons leading to problems in university-enterprise cooperation,the paper explains the collaboration necessity in talents training between demonstrative software school and enterprises,puts forward conventional solutions and probes into the feasible modes of introducing resources from different channels.In addition,analysis is also made into possible stages in which problems might arise. 展开更多
关键词 demonstrative software SCHOOL university-enterprise COOPERATION training TAILORED to ENTERPRISE NEED
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Teaching Reform and Practice of Augmented Reality Course at Xiamen University
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作者 Shihui Guo Weiqiang Lin +1 位作者 Juanjuan Huang Minghong Liao 《计算机教育》 2020年第12期60-65,共6页
Augmented Reality(AR)is a new mode of interactive media that is becoming increasingly popular.Few universities,however,offer courses specifically in AR.This raises questions on appropriate teaching paradigms for this ... Augmented Reality(AR)is a new mode of interactive media that is becoming increasingly popular.Few universities,however,offer courses specifically in AR.This raises questions on appropriate teaching paradigms for this fast-developing area.This paper,therefore,discusses current challenges for teaching AR including a shortage of teaching staff,segmented teaching materials,and theory-driven assessment methods.As such,we present several strategies currently used in the AR technology and application course at Xiamen University.To improve teaching performance,we introduced part-time tutors who are currently employed within the AR industry.Furthermore,we improved the systematic presentation of our course materials to allow an integrative understanding of them.Students developed AR projects using Huawei,the most popular mobile platform in China.This paper concludes that students who underwent this teaching paradigm developed effective skills and knowledge in AR and successfully advanced their careers. 展开更多
关键词 augmented reality teaching reform and practice university-enterprise cooperation model
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Mode Analysis and Deepening Path of the Collaborative Education between Local Engineering Universities and Enterprises
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作者 Zhe Zhao Dan Song 《Journal of Educational Theory and Management》 2018年第3期65-69,共5页
At present, China's local engineering universities have basically formed five modes of collaborative education between Engineering Universities and Enterprises, namely the mode relied on university-enterprise alli... At present, China's local engineering universities have basically formed five modes of collaborative education between Engineering Universities and Enterprises, namely the mode relied on university-enterprise alliance, the mode centered on the "Excellent Engineer Education and Training Program", the mode rooted in key disciplines, the mode based on the innovation and entrepreneurship education activities and the mode of carrying out international joint training, etc. However, in the process of university -enterprise collaborative education, there are still some shortcomings such as long-term deficiency, low fit and insufficient system. Therefore, three main ways to deepen the university-enterprise collaborative education are proposed: The first is to improve and implement the local government's policy guarantee system and incentive measures, fully mobilize and stimulate the enthusiasm and initiative of enterprises to participate in university -enterprise collaborative education;The second is to innovate the long-tenn operation mechanism of university-enterprise collaborative education, and to open up the last mile of educating and employing people;The third is to innovate the university-enterprise joint university-running mode and build a system of engineering talents that integrates innovation and practical capabilities. 展开更多
关键词 LOCAL ENGINEERING UNIVERSITIES university-enterprise collaborative education Mode PATH
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The Detailed Countermeasures for the Excellent Program in the University-enterprise Cooperation
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作者 Hongying Liu 《好家长》 2019年第21期86-86,共1页
1. Establish a flexible entry and exit mechanism for the students and the enterprises.Implementation of Excellence program is a long term project for companies and students, which would meet a lot of variables.From th... 1. Establish a flexible entry and exit mechanism for the students and the enterprises.Implementation of Excellence program is a long term project for companies and students, which would meet a lot of variables.From the business perspective, it is the economic organization to make profits for the purpose. 展开更多
关键词 COUNTERMEASURES university-enterprise COOPERATION
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Exploring the Gaps between Master's Degree Holders' Competence and Industrial Demands in Urban and Rural Planning: Senior Field Engineers' Observations and Reflexive Narrative
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作者 Chuan Ping Xiaoshuo Zheng 《教育技术与创新》 2022年第2期13-21,共9页
This paper examines the academic-work gap in terms of performance reality and performance expectations by expanding on the cultivation model of Master's students in the university context and the most recent indus... This paper examines the academic-work gap in terms of performance reality and performance expectations by expanding on the cultivation model of Master's students in the university context and the most recent industry requirements for talents'competence.The subjects of this paper are Master's degree holders,both academic and professional.The paper examines common issues encountered by enterprise professionals when employing Master's degree holders and proposes a university-enterprise joint cultivation model to achieve win-win outcomes for both higher education institutions and businesses. 展开更多
关键词 Urban and rural planning Graduates with an academic Master’s degree Graduates with a professional Master’s degree university-enterprise joint cultivation model
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Effect of zinc substitution in hydroxyapatite coating on osteoblast and osteoclast differentiation under osteoblast/osteoclast co-culture 被引量:5
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作者 Guolong Meng Xiaoli Wu +3 位作者 Ruijuan Yao Jing He Wu Yao Fang Wu 《Regenerative Biomaterials》 SCIE 2019年第6期349-359,共11页
Zinc is an essential trace element required for bone remodelling process,but its role in such process remains to be elucidated.In particular,inconsistent results have been reported on the effect of Zn on osteoclastic ... Zinc is an essential trace element required for bone remodelling process,but its role in such process remains to be elucidated.In particular,inconsistent results have been reported on the effect of Zn on osteoclastic responses,and supplement of receptor activator of nuclear factor kappa-B ligand(RANKL)factors has been commonly adopted.Co-culture is a suitable approach to elucidating the role of Zn in bone remodelling process,by better imitating the cellular environment as the presence of osteoblasts plays critical role in modulating osteoclastic functions.In this study,zincsubstituted HA coatings have been deposited using a liquid precursor plasma spraying process at two different concentrations(1,2 wt.%).The effect of zinc substitution on osteoblastic and osteoclastic differentiation has been studied in vitro.In particular,a cultivation regime was designed to first induce osteoblastic differentiation of rat bone marrow stromal cells(BMSCs)for 14 days,and then induce osteoclastic differentiation of osteoclast-like precursor RAW 264.7 cells through the aid of the osteoblasts formed for additional 14 days,in the absence of the external addition of RANKL.The results showed that Zn substitution moderately promoted the BMSC differentiation into the osteoblasts and reduced the osteoclastic activity in early time(1 day co-culture).However,promotion of the osteoclastic activity were observed at later stages,as indicated by the significantly enhanced expressions of trap5b and IL-1(8-and 15-day co-culture)and moderate stimulation of the nucleus integration and formation of the multinucleated cells(14-day co-culture).Such stimulating effect of the osteoclastic activity was absent under mono-culture of RAW 264.7 cell,with simple RANKL supplementation.The results suggest that both the zinc and the presence of MSC/osteoblast play profound and highly interacted roles on osteoclast differentiation and activity,which is critical in modulating the bone remodelling process. 展开更多
关键词 ZINC bone marrow stromal cell osteoclast precursor OSTEOCLAST co-cultivation bone remodelling process
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Viability of combining microalgae and macroalgae cultures for treating anaerobically digested piggery effluent
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作者 Ashiwin Vadiveloo Emeka Godfrey Nwoba Navid Reza Moheimani 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2019年第8期132-144,共13页
Algal phytoremediation represents a practical green solution for treating anaerobically digested piggery effluent(ADPE). The potential and viability of combining microalgae and macroalgae cultivation for the efficient... Algal phytoremediation represents a practical green solution for treating anaerobically digested piggery effluent(ADPE). The potential and viability of combining microalgae and macroalgae cultivation for the efficient treatment of ADPE were evaluated in this study.Bioprospecting the ability of different locally isolated macroalgae species illustrated the potential of Cladophora sp. to successfully grow and treat ADPE with up to 150 mg/L NH_4^+ with a biomass productivity of(0.13 ± 0.02) g/(L·day) and ammonium removal rate of(10.23 ± 0.18) mg/(L·day) NH_4^+. When grown by itself, the microalgae consortium used in this study consisting of Chlorella sp. and Scenedesmus sp. was found to grow and treat undiluted ADPE(up to 525 mg/L NH_4^+) with an average ammonium removal rate of 25 mg/(L·day) NH_4^+ and biomass productivity of(0.012 ± 0.0001) g/(L·day). Nevertheless, when combined together, despite the different cultivation systems(attached and non-attached) evaluated,microalgae and macroalgae were unable to co-exist together and treat ADPE as their respective growth were inversely related to each other due to direct competition for nutrients and available resources as well as the negative physical interaction between both algal groups. 展开更多
关键词 Phytoremediation PIGGERY EFFLUENT co-cultivation CLADOPHORA Chlorella sp. SCENEDESMUS SP
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