Biosynthesis of Unsaturated Fatty Acids via Mortierella Isabellina Cultivated in a Medium Containing Butanol

Mortierella isabellina was found to accumulate large amounts of unsaturated fatty acids when it was grown in a medium containing butanol(5 g/L) and yeast extract(10 g/L) and cultivated at 25 ℃ for 5 d during which additional feeding butanol of 2 g/L was fed after being cultivated for 48 h . The resultant mycelial lipids accounted for 40 1% of the dry mycelia, while about 34% of butanol in the medium was converted. The mycelial lipids contained four kinds of unsaturated fatty acids, i.e ., palmitoleic(4 9%), oleic(54 1%), linoleic(10 4%) and linolenic(5 4%) acids. Those accounted for 74 8% of the total fatty acids. The effects of the culture conditions, such as cultivation temperature, initial pH of the medium and additional feeding butanol in the course of cultivation, on the production of mycelial lipids by M. isabellina were studied.

Serum bile acid and unsaturated fatty acid profiles of non-alcoholic fatty liver disease in type 2 diabetic patients

BACKGROUND The understanding of bile acid(BA)and unsaturated fatty acid(UFA)profiles,as well as their dysregulation,remains elusive in individuals with type 2 diabetes mellitus(T2DM)coexisting with non-alcoholic fatty liver disease(NAFLD).Investigating these metabolites could offer valuable insights into the pathophy-siology of NAFLD in T2DM.AIM To identify potential metabolite biomarkers capable of distinguishing between NAFLD and T2DM.METHODS A training model was developed involving 399 participants,comprising 113 healthy controls(HCs),134 individuals with T2DM without NAFLD,and 152 individuals with T2DM and NAFLD.External validation encompassed 172 participants.NAFLD patients were divided based on liver fibrosis scores.The analytical approach employed univariate testing,orthogonal partial least squares-discriminant analysis,logistic regression,receiver operating characteristic curve analysis,and decision curve analysis to pinpoint and assess the diagnostic value of serum biomarkers.RESULTS Compared to HCs,both T2DM and NAFLD groups exhibited diminished levels of specific BAs.In UFAs,particular acids exhibited a positive correlation with NAFLD risk in T2DM,while theω-6:ω-3 UFA ratio demonstrated a negative correlation.Levels ofα-linolenic acid andγ-linolenic acid were linked to significant liver fibrosis in NAFLD.The validation cohort substantiated the predictive efficacy of these biomarkers for assessing NAFLD risk in T2DM patients.CONCLUSION This study underscores the connection between altered BA and UFA profiles and the presence of NAFLD in individuals with T2DM,proposing their potential as biomarkers in the pathogenesis of NAFLD.

Effect of dietary soybean oil and antioxidants on fatty acids and volatile compounds of tail subcutaneous and perirenal fat tissues in fattening lambs

Background： Fat is the primary source of the volatiles that determine the characteristic flavors of animal products.Because unsaturated fatty acids（UFAs） contribute to changes in flavor as a result of the oxidation process, a feeding trial was performed to investigate the effects of dietary soybean oil or antioxidants on the fatty acid and volatile profiles of the tail subcutaneous（SF） and perirenal fat tissues（PF） of fattening lambs. Thirty-six Huzhou lambs were assigned to four dietary treatments in a randomized block design. The lambs＇ diets were supplemented with soybean oil（0 or 3 % of DM） or antioxidants（0 or 0.025 % of DM）.Results： Neither soybean oil nor antioxidant supplementation had an effect on lamb growth（P 〉 0.05）. In regard to tail SF, soybean oil supplementation increased the 18：2n6t（P 〈 0.05） and the total amount of volatile acids,whereas antioxidant supplementation increased the content of C18：2n6c and C18：3n3（P 〈 0.05） but had no effect on the volatiles profile. In regard to PF, dietary soybean oil supplementation increased the C18：0 content（P 〈 0.01）;decreased the C18：1（P = 0.01）, C22：1 n9（P 〈 0.01） and total UFA（P = 0.03） contents; and tended to decrease the E-2-octenal（P = 0.08）, E, E-2, 4-decadienal（P = 0.10）, 2-undecenal（P = 0.14） and ethyl 9-decenoate（P = 0.10） contents.Antioxidant supplementation did not affect either the fatty acid content or the volatiles profile in the PF.Conclusions： Tail SF and PF responded to dietary soybean oil and antioxidant supplementation in different ways. For SF, both soybean oil and antioxidant supplementation increased the levels of unsaturated fatty acids but triggered only a slight change in volatiles. For PF, soybean oil supplementation decreased the levels of unsaturated fatty acids and oxidative volatiles, but supplementation with antioxidants had little effect on PF fatty acids and the volatiles profile.

Branched chain amino acids alter fatty acid profile in colostrum of sows fed a high fat diet

Background:Branched chain amino acids(BCAAs)are important substrates for milk protein synthesis in the mammary gland,and are tightly related to lipid metabolism.No study has been performed examining the role of BCAAs with high fat diets on milk fat synthesis.This study was designed to investigate the effect of dietary BCAAs on growth performance of piglets,progeny body weight,and milk fat composition in sows fed a high fat diet.Four diets(CON=control diet;HF=high fat diet with 8%soybean oil;HF-MB=HF plus 0.39%BCAAs;HF-HB=HF plus 0.78%BCAAs)were fed to sows from late gestation to weaning.Results:Compared to HF,BCAAs(HF-MB and HF-HB)increased the litter weight(P<0.05)and overall litter weight gain(P<0.05)at weaning and increased colostrum fat content by 27.3–35.8%(P<0.01).Fatty acid profiles between the two doses of BCAAs were similar.Compared with HF,HF-MB tended to decrease the percentage of C18:3n3(P=0.063)and increased the percentage of C18:1n9c(P=0.03).In addition,BCAAs in HF-MB increased the concentration of total fatty acid by 22.1%in colostrum(P=0.03)but decreased that in serum at parturition by 53.2%(P=0.027).The fatty acids in colostrum that increased with BCAAs were C15:0,C17:0,C20:3n6,C20:4n6,C20:5n3 and C22:6n3(P=0.00~0.04).Colostrum fatty acids of C20:0,C21:0,C22:0,C16:1,C20:1,C18:1n9c also tended to be increased(0.05<P<0.1)with BCAAs.The change in sow serum fatty acid profile due to BCAAs was different from that in colostrum.Conclusions:BCAAs in high fat diet of sows altered the fatty acid composition in colostrum and enhanced litter growth.Our study indicated that BCAAs supplementation can enhance mammary fatty acid uptake and mammary fat synthesis and that supplemental BCAAs and fat in late gestation and lactation diets for sows can improve reproductive performance.

Growth Response and Fatty Acid Composition of Juvenile Procambarus clarkii Fed Different Sources of Dietary Lipid

An experiment was conducted to evaluate the effects of feeding various sources of dietary lipid on weight gain, feed conversion, survival and fatty acid composition of juvenile red swamp crawfish, Procam-barus clarkii. Six semi-purified diets containing vitamin-free casein, defatted soybean meal, 0. 5% choline chloride, 0. 5% glycine and 0. 5% cholesterol were supplemented with 6. 0% of either anchovy oil, linseed oil, soybean oil, rapeseed oil, safflower oil or pork lard. Each diet was fed to crawfish (3. 07±0. 21 g average weight) in three replicate aquaria for 60 days. Survival rate, weight gain and feed conversion were best for crawfish fed the diet containing anchovy oil. Crawfish fed the linseed oil diet had the second highest weight gain, followed by crawfish on soybean oil, repaseed oil, safflower oil and pork lard diets, respectively. Feed conversion values were a reflection of weight gain. Results showed that both n-6 and n-3 fatty acids are dietary essential for juvenile Procambarus clarkii, although n-3 fatty acids promoted faster growth than n-6. However, highly unsaturated fatty acids (HUFA) (20 : 5n-3 and 22 :6n-3) had better growth-promoting effect than 18 : 3n-3, due probably to the limited ability of crawfish to bioconvert fatty acids to polyenoic forms of longer chain length. The fatty acid composition of the crawfish generally reflected that of the dietary lipids, especially for the diets containing unsaturated fatty acids.

Protective role of concomitant administration of flax lignan concentrate and omega-3-fatty acid on myocardial damage in doxorubicin-induced cardiotoxicity

The severe cardiotoxicity incurred due to doxorubicin limits the use of their therapeutic potential.The current study aims to investigate the cardioprotective effect of concomitant administration of flax lignan concentrate(FLC)and omega-3-fatty acid(ω-3-FA)on myocardial damage in doxorubicin-induced cardiotoxicity in Wistar rats.Cardiotoxicity was induced by intraperitoneal injection of doxorubicin(4 mg/kg)on day 7th,14th,21st and 28th day in normal saline.Concomitant administration of FLC(500 mg/kg)andω-3-FA(1 mL/kg)lowered TNF-αlevel,normalized ST,QT and mean arterial blood pressure,elevation in endogenous enzymes levels such as glutathione and lowering in malondialdehyde,super oxide dismutase followed by normalized lipid profile and reduced the mortality rate.The treatment had antiapoptotic potential at cellular level also histopathology of heart tissue(light and electron microscopical).Thus concomitant action of FLC andω-3-FA may be antioxidant,antihyperlipidemic,anti-inflammatory and anti-apoptotic actions seem to the probable mechanisms in doxorubicin induced cardiotoxicity.It can be concluded that FLC andω-3-FA both have distinct mechanism for cardioprotection and hence the additive effect was observed in the present study due to concomitant administration of FLC andω-3-FA.©2013 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved.

Changes in Unsaturated Levels of Fatty Acids in Thylakoid PSII Membrane Lipids During Chilling-induced Resistance in Rice

Temperature is one of the abiotic factors limiting growth and productivity of plants. In the present work, the effect of low non-freezing temperature, as an inducer of ＂chilling resistance＂, was studied in three cultivars of rice （Oryza sativa L.）, japonica cv. 9516 （j-9516）, the two parental lines of superhigh-yield hybrid rice between subspecies, Peiai/E32 （ji-PE）, and the traditional indica hybrid rice Shanyou 63 （i-SY63）. Leaves of chill-treated rice showed chilling-induced resistance, as an increase of their low-temperature tolerance was measured using chlorophyll fluorescence measurements, revealing a change in photosystem II （PSII） efficiency. After 5 d of exposure to 11 ~C under low light （100 pmol·m^-2·s^-1）, levels of unsaturated fatty acids in PSII thylakoid membrane lipids decreased during the initial 1-2 d, then increased slowly and reached 99.2%, 95.3% and 90.1% of the initial value （0 d） in j-9516, ji-PE and i-SY63, respectively, on the fifth day. However, under medium light （600 μmol·m^-2·s^-1）, all cultivars experienced similar substantial photoinhibition, which approached steady state levels after a decline in levels of unsaturated fatty acids in PSII thylakoid membrane lipids to about 57.1%, 53.8% and 44.5% of the initial values （0 d） in j-9516, ji-PE and i-SY63 on the fifth day. Under either chilling-induced resistance （the former） or low temperature photoinhibition （the latter） conditions, the changes of other physiological parameters such as D1 protein contents, electron transport activities of PSII （ETA）, Fv/Fm, xanthophyl cycle activities expressed by DES （deepoxide state） were consistent with that of levels of unsaturated fatty acids in PSII thylakoid membrane lipids. So there were negative correlations between saturated levels of fatty acids （16：1（3t）, 16：0, 18：0）, especially the 16：1（3t） fatty acid on thylakoid membrane and other physiological parameters, such as D1 protein contents, ETA and （A＋Z）/（A＋V＋Z）. A specific role of desaturation of fatty acids and the photoprotective pigments of the xanthophyl cycle, leading to an acclimation response in thylakoid membrane lipids may be involved. We conclude that chilling-induced resistance is accelerated by the unsaturation of thylakoid membranes, and the ability of rice plants to cold-harden can be enhanced by genetic engineering.

The AD/HD Syndrome as a Group of Biological Disorders

Problem: There is a need to collect the many and varied data on AD/HD (Attention deficit hyper-activity disorder) into a meaningful overview. Method: Based on peer reviewed and published data as well as own research we try to make sense of the physiological mechanisms resulting in the relevant symptoms. Conclusion: AD/HD clearly has a genetic disposition, but as with many other genetic syndromes, the resulting proteomics must be stressed to become manifest as disease/ disorder. A common trait in the different etiologies is lower arousal.

Acetylenes and fatty acids from Codonopsis pilosula

Four new acetylenes(1–4) and one new unsaturated ω-hydroxy fatty acid(5),together with 5known analogues,were isolated from an aqueous extract of Codonopsis pilosula roots.Their structures were determined by spectroscopic and chemical methods.The new acetylenes are categorized as an unusual cyclotetradecatrienynone(1),tetradecenynetriol(2),and rare octenynoic acids(3 and 4),respectively,and 3 and 4 are possibly derived from oxidative metabolic degradation of 1 and/or 2.The absolute configuration of 1 was assigned by comparison of the experimental circular dichroism(CD)spectrum with the calculated electronic circular dichroism(ECD) spectra of stereoisomers based on the quantum-mechanical time-dependent density functional theory,while the configuration of 2 was assigned by using modified Mosher's method based on the MPA determination rule of ΔδRSvalues for diols.

Effects of the ratio of unsaturated fatty acid to saturated fatty acid on the growth performance,carcass and meat quality of finishing pigs

The effects on finishing pigs(80-100 kg BW) fed diets supplemented with oil sources containing different ratios of unsaturated to saturated fatty acids(UFA:SFA ratio) were evaluated in 15 barrows and15 gilts(Duroc × Large White × Landrace). Three experimental diets were evaluated using a randomized complete block design, with broken rice, soybean meal and rice bran as the main feedstuffs in the control diet. Diets 2 and 3 consisted of the control diet supplemented with 3% oil, with UFA:SFA ratios of 2.5:1 and 5:1, respectively. Overall, there was no significant difference(P> 0.05) found in the average daily gain(ADG) of the pigs fed the treatment diets; however, the pigs fed the control diet and diet 3 had better(P < 0.05) feed conversion ratios(FCR) than the pigs fed diet 2. The pigs fed diets 2 and 3, which were supplemented with oil at UFA:SFA ratios of 2.5:1 and 5:1, had greater(P < 0.05) average daily feed intakes(ADFI) than the pigs in the control group. Additionally, it was found that the gender of the pigs had an effect(P < 0.05) on the FCR. Interaction effects between the experimental diets and the gender of the pigs(P < 0.05) were found in the ADFI and FCR. There were no significance differences(P > 0.05)among the treatment groups with regard to the carcass quality of the pigs; however, it was found that the gilts had greater(P < 0.01) loin eye areas than the barrows fed diets 2 and 3 and the loin eye area of pig fed diet 2 was the largest(P < 0.05); In the case of the meat quality parameters, it was clearly found that the pigs fed the control diet had a greater(P < 0.05) lightness(L~*) in the meat colour, and the lowest cooking loss was found in the pigs fed the diet supplemented with fat containing the UFA:SFA ratio of 5:1.Overall, the dietary treatment did not significantly affect the drip loss, thawing loss and shear force of the pork. In conclusion, the supplementation of oil with UFA:SFA ratios of 2.5:1 and 5:1 has the potential to improve pork quality.

METABOLISM -OF LOW DENSITY LIPOPROTEIN-DEPENDENT UNSATURATED FATTY ACID IN CULTURED CELLS

The traditional view of the regulation of eicosanoid formation holds that agonists have to activate receptor-coupled phospholipases before eicosanoid syntheiss can be initiated. Action of phospholipases results in an increase in the intracellular concentration of unesterified arachidonic acid (AA). Once unesterified AA is present in sufficient amounts it can be metabolized by a variety of enzymes that catalyze

Research Status of Alpha Linolenic Acid(ALA) and Its Application Advances in Livestock and Poultry Production

Alpha linolenic acid （ ALA）, a kind of polyunsaturated fatty acid extracted from plants and fruits, has a variety of biological functions. In recent years, ALA has attracted much attention as a natural green feed additive. The physical and chemical properties, metabolic process, physiological function of ALA as well as its application and mechanisms in livestock and poultry production are summarized in the paper.

CHH methylation of genes associated with fatty acid and jasmonate biosynthesis contributes to cold tolerance in autotetraploids of Poncirus trifoliata

Polyploids have elevated stress tolerance,but the underlying mechanisms remain largely elusive.In this study,we showed that naturally occurring tetraploid plants of trifoliate orange(Poncirus trifoliata(L.) Raf.) exhibited enhanced cold tolerance relative to their diploid progenitors.Transcriptome analysis revealed that whole-genome duplication was associated with higher expression levels of a range of well-characterized cold stress-responsive genes.Global DNA methylation profiling demonstrated that the tetraploids underwent more extensive DNA demethylation in comparison with the diploids under cold stress.CHH methylation in the promoters was associated with up-regulation of related genes,whereas CG,CHG,and CHH methylation in the 3’-regions was relevant to gene down-regulation.Of note,genes involved in unsaturated fatty acids(UFAs) and jasmonate(JA)biosynthesis in the tetraploids displayed different CHH methylation in the gene flanking regions and were prominently up-regulated,consistent with greater accumulation of UFAs and JA when exposed to the cold stress.Collectively,our findings explored the difference in cold stress response between diploids and tetraploids at both transcriptional and epigenetic levels,and gained new insight into the molecular mechanisms underlying enhanced cold tolerance of the tetraploid.These results contribute to uncovering a novel regulatory role of DNA methylation in better cold tolerance of polyploids.

Synthesis of unsaturated phosphatidyl inositol-3,4,5-trisphosphate

Phosphatidylinositol polyphosphate (PIPx) are related with tyrosine kinase activation and cell proliferation and carcinogenesis. PIPx is an important target compound for synthetic organic chemist. PIPx having saturated fatty acid components have been reported. Recently we have succeeded in the synthesis of phosphatydylinositol-3,4,5-trisphosphate with unsaturated fatty acid component by the reaction of diethyl chlorophosphite with 1,2-di-O-cyclohexylidene-3,4-0-(tetraisopropyldisiloxane 1,3-diyl)-6-O-(4-oxopentanoyl)-myo-inositol.

Cytochrome b5 Reductase 1 Triggers Serial Reactions that Lead to Iron Uptake in Plants

Rhizosphere acidification is essential for iron （Fe） uptake into plant roots. Plasma membrane （PM） H＊-ATPases play key roles in rhizosphere acidification. However, it is not fully understood how PM H＋-ATPase activity is regulated to enhance root Fe uptake under Fe-deficient conditions. Here, we present evidence that cytochrome b5 reductase 1 （CBR1） increases the levels of unsaturated fatty acids, which stimulate PM H＋-ATPase activity and thus lead to rhizosphere acidification. CBRl-overexpressing （CBRI-OX） Arabidopsis thaliana plants had higher levels of unsaturated fatty acids （18：2 and 18：3）, higher PM H＊-ATPase activity, and lower rhizosphere pH than wild-type plants. By contrast, cbrl loss-of-function mutant plants showed lower levels of unsaturated fatty acids and lower PM H＊-ATPase activity but higher rhizosphere pH. Reduced PM H＊-ATPase activity in cbrl could be restored in vitro by addition of unsatu- rated fatty acids. Transcript levels of CBR1, fatty acids desaturase 2 （FAD2）, and fatty acids desaturase 3 （FAD3） were increased under Fe-deficient conditions. We propose that CBR1 has a crucial role in increasing the levels of unsaturated fatty acids, which activate the PM H＊-ATPase and thus reduce rhizosphere pH. This reaction cascade ultimately promotes root Fe uptake.

Alteration of membrane lipid biophysical properties and resistance of human lung adenocarcinoma A_(549) cells to cisplatin

Alterations of membrane lipid biophysical properties of sensitiveA549 and resistant A549/DDP cells to the Cis-dichlorodiammine platinum (Cisplatin) were performed by measurements of fluorescence and flow cytometry approaches using fluorescence dyes of DPH, N-AS and Merocyanine 540 (MC 540) respectively. Fatty acids of membrane lipid of the two cell lines were analyzed by gas chromatography. The results indicated clearly that fluorescence polarization (P) of the DPH probe is 0.169 for the sensitive A549 cell and 0.194 for the resistant A549/DDP cells. Statistical analysis showed significant difference between the two cell lines. The polarizations of 2-AS and 7-AS which reflect the fluidity of surface and middle of lipid bilayer are 0.134 and 0.144 for the sensitive A549 cells as well as 0.171 and 0.178 for the resistant A549/DDP cells respectively, but there is no significant difference of the polarization of 12-AS between the two cell lines. This shows that altera-tions of the membrane fluidity of both cells were mainly located on the surface and middle of the lipid bilayer. In addition, the packing density of phospholipid molecules in the membrane of the two cell lines detected by MC540 probe indicated that lipid packing of A549 cell membranes was looser than that of the A549/DDP cells. And unsaturation degree of plasma membrane fatty acids of the A549/DDP cells was also lower than that of A549 cells. Taken together, it was proposed that the al-teration of membrane lipid biophysical state may be involved in the resistance of A549/DDP cells to cisplatin.