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Measurement of very low-molecular weight metabolites by traveling wave ion mobility and its use in human urine samples
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作者 Alongkorn Kurilung Suphitcha Limjiasahapong +9 位作者 Khwanta Kaewnarin Pattipong Wisanpitayakorn Narumol Jariyasopit Kwanjeera Wanichthanarak Sitanan Sartyoungkul Stephen Choong Chee Wong Nuankanya Sathirapongsasuti Chagriya Kitiyakara Yongyut Sirivatanauksorn Sakda Khoomrung 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2024年第5期733-743,共11页
The collision cross-sections(CCS)measurement using ion mobility spectrometry(IMS)in combination with mass spectrometry(MS)offers a great opportunity to increase confidence in metabolite identification.However,owing to... The collision cross-sections(CCS)measurement using ion mobility spectrometry(IMS)in combination with mass spectrometry(MS)offers a great opportunity to increase confidence in metabolite identification.However,owing to the lack of sensitivity and resolution,IMS has an analytical challenge in studying the CCS values of very low-molecular-weight metabolites(VLMs250 Da).Here,we describe an analytical method using ultrahigh-performance liquid chromatography(UPLC)coupled to a traveling wave ion mobility-quadrupole-time-of-flight mass spectrometer optimized for the measurement of VLMs in human urine samples.The experimental CCS values,along with mass spectral properties,were reported for the 174 metabolites.The experimental data included the mass-to-charge ratio(m/z),retention time(RT),tandem MS(MS/MS)spectra,and CCS values.Among the studied metabolites,263 traveling wave ion mobility spectrometry(TWIMS)-derived CCS values(TWCCSN2)were reported for the first time,and more than 70%of these were CCS values of VLMs.The TWCCSN2 values were highly repeatable,with inter-day variations of<1%relative standard deviation(RSD).The developed method revealed excellent TWCCSN2 accuracy with a CCS difference(DCCS)within±2%of the reported drift tube IMS(DTIMS)and TWIMS CCS values.The complexity of the urine matrix did not affect the precision of the method,as evidenced by DCCS within±1.92%.According to the Metabolomics Standards Initiative,55 urinary metabolites were identified with a confidence level of 1.Among these 55 metabolites,53(96%)were VLMs.The larger number of confirmed compounds found in this study was a result of the addition of TWCCSN2 values,which clearly increased metabolite identification confidence. 展开更多
关键词 Very low-molecular-weight metabolites Traveling wave ion mobility Collision cross-section Human urine
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Effects of Different Diuretics on Water Drinking Amount, Urination Volume and Related Blood Indices of Sheep Fed with Cottonseed 被引量:1
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作者 郭璇 邵伟 +1 位作者 李艳丽 余雄 《Agricultural Science & Technology》 CAS 2013年第2期345-347,353,共4页
[Objective] This study aimed to investigate the effects of three kinds of diuretics on the water drinking amount and urination volume of sheep fed with cottonseed hulls. [Method] The method of self-control was adopted... [Objective] This study aimed to investigate the effects of three kinds of diuretics on the water drinking amount and urination volume of sheep fed with cottonseed hulls. [Method] The method of self-control was adopted. Five two-year-old male Kazak sheep about 40 kg which never had intake of the feeds containing gossypol were selected. The experiment was divided into five periods, namely the control period, a period with the supplement of 300 mg/d of hydrochlorothiazide, a period with the supplement of 200 mg/d of hydrochlorothiazide, a period with the supplement of 350 mg of GY-01 and a period with the supplement of 200 mg of GY-01. The sheep were fed with the concentrate and the cottonseed hulls in the control period, and corresponding diuretics were supplemented in the rest four periods. The water drinking amount, urination volume and the contents of the hormone and ion in the blood were determined in each period. [Result] After 350 mg GY-01 was supplemented, the water drinking amount of each sheep had an increase of 60.14% compared with that of the sheep in the control period and the urination volume was 2.67 times of that of the latter, and the differences were both significant (P0.01). The antidiuretic hormone declined by 32.59% compared with that of the sheep in the control period, with the significant difference (P 0.05). The adrenocorticotropic hormone content increased by 81.93% compared with that of the sheep in the control period and the difference was significant (P0.01). An increase of 28.04% (P 0.05) and 39.39% (P 0.01) was found in the contents of serum potassium and phosphorus respectively compared with that of sheep in the control period. [Conclusion] The diet with supplement of GY-01 can increase the urination volume of livestock fed with the cottonseed hulls, and the most appropriate supplement amount is 200 mg GY-01. 展开更多
关键词 Cottonseed hulls SHEEP DIURETICS urination volume Hormone Inorganic ions
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Prevalence of Thyroid Nodules and Its Relationship with Iodine Status in Shanghai:a Population-based Study 被引量:27
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作者 SONG Jun ZOU Shu Rong +8 位作者 GUO Chang Yi ZANG Jia Jie ZHU Zhen Ni MI Ming HUANG Cui Hua YU Hui Ting LU Xi RUAN Ye WU Fan 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2016年第6期398-407,共10页
Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Meth... Objective This study was designed to evaluate the prevalence of thyroid nodules(TNs) and its relationship with urine iodine concentrations(UICs) after the regional rapid economic growth and lifestyle changes. Methods A cross-sectional survey was conducted in the general population aged 15-69 years. A questionnaire regarding general and personal characteristics and relevant information was administered. Ultrasonography of the thyroid was performed, and serum triiodothyronine(T3), tetraiodothyronine(T4), serum thyroid stimulating hormone(TSH), free triiodothyronine(FT3), free tetraiodothyronine(FT4), thyroglobulin antibody(Tg Ab), thyroid peroxidase antibody(TPOAb), and TSH receptor antibody(TRAb) levels were measured for each individual subject. Results The prevalence rates of TNs in the whole population, females and males were 27.76%, 34.04%, and 21.60%, respectively. The prevalence of multiple nodules increased with age, whereas the prevalence peaks differed between males and females. The median UICs in the whole population and females with non-TNs were higher than those of subjects with TNs(P=0.0035, P=0.0068). The median UICs in subjects with a single TN were higher than those in subjects with multiple TNs(P=0.0164, P=0.0127). The result showed a U-shaped curve relationship between UIC and prevalence of TNs. The prevalence of TNs was the lowest when the UIC was 140-400 μg/L. Conclusion The prevalence of TNs was nearly 30% and increased with age. The relationship between UIC and prevalence of TNs is U-shaped, with an increase in risk when the UIC was 〈140 μg/L and 〉400 μg/L. Very low or high UIC levels need attention and correction. 展开更多
关键词 Thyroid Nodules PREVALENCE Urine iodine EPIDEMIOLOGY
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Biotransformation of aesculin by human gut bacteria and identification of its metabolites in rat urine 被引量:5
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作者 Wei-Jun Ding Yun Deng +5 位作者 Hao Feng Wei-Wei Liu Rong Hu Xiang Li Zhe-Ming Gu Xiao-Ping Dong 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第12期1518-1523,共6页
AIM: To observe the biotransformation process of a Chinese compound, aesculin, by human gut bacteria, and to identify its metabolites in rat urine.METHODS: Representative human gut bacteria were collected from 20 he... AIM: To observe the biotransformation process of a Chinese compound, aesculin, by human gut bacteria, and to identify its metabolites in rat urine.METHODS: Representative human gut bacteria were collected from 20 healthy volunteers, and then utilized in vitro to biotransform aesculin under anaerobic conditions. At 0, 2, 4, 8, 12, 16, 24, 48 and 72 h postincubation, 10 mL of culture medium was collected. Metabolites of aesculin were extracted 3 × from rat urine with methanol and analyzed by HPLC. For in vivo metabolite analysis, aesculetin (100 mg/kg) was administered to rats via stomach gavage, rat urine was collected from 6 to 48 h post-administration, and metabolite analysis was performed by LC/ESI-MS and MS/MS in the positive and negative modes.RESULTS: Human gut bacteria could completely convert aesculin into aesculetin in vitro. The biotransformation process occurred from 8 to 24 h post-incubation, with its highest activity was seen from 8 to 12 h. The in vitro process was much slower than the in vivo process. In contrast to the in vitro model, six aesculetin metabolites were identified in rat urine, including 6-hydroxy-7-glucocoumarin(M1), 6-hydroxy-7-sulf-coumarin (M2), 6, 7-digluco-coumarin (M3), 6-glc-7-gluco-coumarin (M4), 6-O-methyl-7-gluco-coumarin (MS) and 6-O-methyl-7- sulf-coumarin (M6). Of which, M2 and M6 were novel metabolites.CONCLUSION: Aesculin can be transferred into aesculetin by human gut bacteria and is further modified by the host in vivo. The diverse metabolites of aesculin may explain its pleiotropic pharmaceutical effects. 展开更多
关键词 Aesculin BIOTRANSFORMATIon Human gutbacteria Rat urine Sulfated derivatives LC/ESI-MS Aseculetin
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The effects of application of an ancient type of acupuncture needle on increase in urination of hospitalized oldest-old people 被引量:3
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作者 Mayumi Watanabe Osamu Takano +6 位作者 Chikako Tomiyama Jianjun Guan Guanhui Hou Hidetoshi Mori Kazushi Nishijo Toru Abo Kohei Akazawa 《Health》 2013年第7期1092-1098,共7页
The di-zhen (DZ) is an ancient type of acupuncture needle with a history dating back to more than 2000 years. Unlike modern acupuncture needles, the DZ is not inserted subcutaneously and is considered to be safe for c... The di-zhen (DZ) is an ancient type of acupuncture needle with a history dating back to more than 2000 years. Unlike modern acupuncture needles, the DZ is not inserted subcutaneously and is considered to be safe for common use, even at the bedside of terminally ill patients. In this case study, DZ application was selected and applied 14 times on a 92-year-old patient who had suffered severe oliguria along with administration of diuretics. Urine volume increased 12 out of 14 times following DZ treatment;however, the other two times it was ineffective. To investigate the reason and the differences in effective/ineffective DZ application, two internal conditions (effective vs ineffective) were compared by analyzing daily nursing reports (vital signs such as blood pressure, urine volume and body temperature) and weekly clinical data (including renal, liver and immune function, urea nitrogen, creatinine, ALT, AST, the ratio and number of leukocytes, erythrocytes). DZ application increased urine volume via a transient modification of the autonomic nervous system when internal homeostasis was maintained. Aging populations, who often have several diseases and medications, are rapidly increasing in developed countries. Therefore, the study and application of DZ may contribute to a reduction in medical costs, which have been rapidly and heavily expanding recently. 展开更多
关键词 Di-Zhen (DZ) Acupuncture OLDEST-OLD AUTonOMIC Nervous System (ANS) LEUKOCYTE urination Terminal Care
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Study on Fluorescence Property of Sparfloxacin Derivatizing System and its Application 被引量:1
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作者 Du, LM Zhou, J +2 位作者 Xu, QQ Fan, ZF Yuan, R 《Chinese Chemical Letters》 SCIE CAS CSCD 2000年第3期255-258,共4页
In acid medium, sparfloxacin is oxidized by nitrous acid, then reacts with hydrobromic acid to form a new fluorescence substance, which can emit the strong fluorescence. which is 151 fold more than that of sparfloxaci... In acid medium, sparfloxacin is oxidized by nitrous acid, then reacts with hydrobromic acid to form a new fluorescence substance, which can emit the strong fluorescence. which is 151 fold more than that of sparfloxacin itself. By this, a new sensitive method for the determination of sparfloxacin in human urine by derivative-synchronous fluorescence is presented with good results. 展开更多
关键词 SPARFLOXACIN nitrous acid derivative-synchronous fluorescence human urine
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Clinical Observation on 50 Cases with Child Neuro Frequent Urination Treated by Acupuncture
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作者 Liu Yi Xiang ShenJie(Anshan Hospital of Women and Children,Liao Ning 114014) 《中国针灸》 CAS CSCD 北大核心 1995年第S2期318-319,共2页
ClinicalObservationon50CaseswithChildNeuroFrequentUrinationTreatedbyAcupuncture¥LiuYiXiang;ShenJie(AnshanHos... ClinicalObservationon50CaseswithChildNeuroFrequentUrinationTreatedbyAcupuncture¥LiuYiXiang;ShenJie(AnshanHospitalofWomenandCh... 展开更多
关键词 ACUPUNCTURE urination Observation Clinical Frequent NEURO CASES TREATED CHILD by
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WHAT POINTS SHOULD BE PAID ATTENTION TO IN THE TCM DIFFERENTIAL TREATMENT OF SENILE PATIENTS WITH FREQUENT URINATION?
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作者 魏子孝 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1997年第1期73-74,共2页
Frequent urination is a commonlyseen symptom in our clinical practice. The degrees of severity may be different as to the various chief complaints presented by patients such as frequent urination, oliguria, emergent u... Frequent urination is a commonlyseen symptom in our clinical practice. The degrees of severity may be different as to the various chief complaints presented by patients such as frequent urination, oliguria, emergent urination, dysuria and vesical tenesmus etc. Among the young and middle-aged patients, it is mostly seen in female urinary tract infection, which is gen- 展开更多
关键词 emergent severity URINARY mostly young frequent Radix CLEARING STOOL urine
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ACUPUNCTURE TREATMENT OF HIRSUTISM AND ITS EFFECT ON THE ENDOCRINOSITY
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作者 吴泽森 蔡信安 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 1989年第3期207-209,共3页
Hirsutism or ectopic polytrichosis comprisestwo categories,congenital and acquired.Clini-cally,most patients pertain to acquired localhypertrichosis.We adopted acupuncture for thetreatment of hypertrichosis in women w... Hirsutism or ectopic polytrichosis comprisestwo categories,congenital and acquired.Clini-cally,most patients pertain to acquired localhypertrichosis.We adopted acupuncture for thetreatment of hypertrichosis in women with goodresults and investigated the levels of 展开更多
关键词 acupuncture ECTOPIC ConGENITAL Observation ENDOCRINE women URINE minutes needle TESTOSTERonE
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Urinary Creatinine Concentrations and Its Explanatory Variables in General Chinese Population:Implications for Creatinine Limits and Creatinine Adjustment
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作者 JI Sai Sai LYU Yue Bin +23 位作者 QU Ying Li HU Xiao Jian LU Yi Fu CAI Jun Fang SONG Shi Xun ZHANG Xu LIU Ying Chun YANG Yan Wei ZHANG Wen Li LI Ya Wei ZHANG Ming Yuan CHEN Chen LI Cheng Cheng LI Zheng GU Heng LIU Ling CAI Jia Yi QIU Tian FU Hui JI S.John ZHAO Feng ZHU Ying CAO Zhao Jin SHI Xiao Ming 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2022年第10期899-910,共12页
Objective The study aimed to analyze the applicability of the World Health Organization’s exclusionary guidelines for Urinary creatinine(Ucr)in the general Chinese population,and to identify Ucr related factors.Metho... Objective The study aimed to analyze the applicability of the World Health Organization’s exclusionary guidelines for Urinary creatinine(Ucr)in the general Chinese population,and to identify Ucr related factors.Methods We conduct a cross-sectional study using baseline data from 21,167 participants in the China National Human Biomonitoring Program.Mixed linear models and restricted cubic splines(RCS)were used to analyze the associations between explanatory variables and Ucr concentration.Results The geometric mean and median concentrations of Ucr in the general Chinese population were 0.90 g/L and 1.01 g/L,respectively.And 9.36%samples were outside 0.3-3.0 g/L,including 7.83%below the lower limit and 1.53%above the upper limit.Middle age,male,obesity,smoking,higher frequency of red meat consumption and chronic kidney disease were associated significantly with higher concentrations of Ucr.Results of the RCS showed Ucr was positively and linearly associated with body mass index,inversely and linearly associated with systolic blood pressure,diastolic blood pressure,triglycerides level,and glomerular filtration rate,and were non-linearly associated with triiodothyronine.Conclusion The age-and gender-specific cut-off values of Ucr that determine the validity of urine samples in the general Chinese population were recommended.To avoid introducing bias into epidemiologic associations,the potential predictors of Ucr observed in the current study should be considered when using Ucr to adjust for variations in urine dilution. 展开更多
关键词 BIOMonITORING URINE Hydration correction CREATININE
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Electrochemical behavior of paclitaxel and its determination at glassy carbon electrode
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作者 Jayant I.Gowda Sharanappa T.Nandibewoor 《Asian Journal of Pharmaceutical Sciences》 SCIE CAS 2014年第1期42-49,共8页
The electrochemical behavior of paclitaxel drug was studied at a glassy carbon electrode in phosphate buffer solutions using cyclic and differential-pulse voltammetric techniques.The oxidation process was shown to be ... The electrochemical behavior of paclitaxel drug was studied at a glassy carbon electrode in phosphate buffer solutions using cyclic and differential-pulse voltammetric techniques.The oxidation process was shown to be irreversible over the pH range(3.0e10.4)and was diffusion controlled.Effects of anodic peak potential(E_(p)),anodic peak current(Ipa),scan rate,pH,heterogeneous rate constant(k^(0)),etc have been discussed.A possible electrooxidation mechanism was proposed.An analytical method was developed for the determination of paclitaxel in phosphate buffer solution at pH¼7.0 as a supporting electrolyte.The anodic peak current varied linearly with paclitaxel concentration in the range 1.0×10^-(6)M to 1.0×10^-(5)M with a limit of detection(LOD)of 1.23×10^(-8)M and limit of quantification(LOQ)of 4.10×10^(-8)M.The proposed method was successfully applied to the determination of paclitaxel in pure and real samples. 展开更多
关键词 PACLITAXEL Glassy carbon electrode VOLTAMMETRY Injection analysis URINE
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Metabolic dynamics in chronic gastritis:Examining urinary profiles post Helicobacter pylori eradication 被引量:1
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作者 Imshaal Musharaf Abdulqadir J Nashwan 《World Journal of Clinical Cases》 SCIE 2024年第16期2698-2700,共3页
Chronic gastritis is the persistent and insidious inflammation of the gastric lining.Helicobacter pylori(H.pylori)has been identified as the most common cause of chronic gastritis and consequently elimination of H.pyl... Chronic gastritis is the persistent and insidious inflammation of the gastric lining.Helicobacter pylori(H.pylori)has been identified as the most common cause of chronic gastritis and consequently elimination of H.pylori can lead to its cure.This editorial explores the use of urinary metabolic profiles before and after eradication to identify biomarkers that can aid in prognosis and treatment.Despite providing promising insights,there are limitations such as a small sample size(17 patients),a narrow treatment period of 2 wk,and treatment heterogeneity,which raise concerns.Nevertheless,these findings have opened a gateway to enhancing the treatment and prognosis of chronic gastritis through urinary metabolomics. 展开更多
关键词 GASTRITIS Helicobacter pylori Chronic gastritis Urine metabolomics Quadruple therapy Precancerous lesions
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Synchronous fluorescence technique and its use in identification of polynuclear aromatic hydrocarbons in urine samples
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作者 Zhao Zhenhua and Quan Wenyi Beijing Municipal Research Insitute of Environmental Protection 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 1989年第2期109-115,共7页
The synchronous fluorescence spectroscopy of three polymiclear aromatic hydrocarbons was studied. It was shown that the specific Δλ of the spectra were 10nm for benzo(k) fluoranthene, 25 nm for benzo(a) pyrene, and ... The synchronous fluorescence spectroscopy of three polymiclear aromatic hydrocarbons was studied. It was shown that the specific Δλ of the spectra were 10nm for benzo(k) fluoranthene, 25 nm for benzo(a) pyrene, and 40 nm for pyrene. The peaks of the spectra were at 407, 404, and 373 nm for the three chemicals, respectively. Benzo(k) fluoranthene, benzo(a) pyrene, and pyrene in the urine samples from smokers were identified by high pressure liquid chromatography combined with the synchronous fluorescence spectroscopy. 展开更多
关键词 polynuclear aromatic hydrocarbons synchronous fluorescence spec-troscopy urine.
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Developing a Questionnaire for Prediction of High Salt Intake Based on Salt Usage Behavior: Analysis from Dietary Habits and Urinary Sodium Excretion in Japan
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作者 Chika Horikawa Nobuko Murayama +14 位作者 Eri Tochikura Emi Ishida Miyo Kojima Sumie Isobe Kazue Suzuki Kyoko Fueta Miyoko Kira Mizue Sasaya Misato Koide Asako Oyanagi Rika Yonekura Yae Iriyama Yumi Nakajima Chiho Hatano Junichi Horii 《Food and Nutrition Sciences》 2017年第1期1-18,共18页
Salt reduction policies have been implemented throughout the world as well as in Japan. However, most of the current questionnaires for estimating salt intake are apt to be based on empirical evidence. This study was ... Salt reduction policies have been implemented throughout the world as well as in Japan. However, most of the current questionnaires for estimating salt intake are apt to be based on empirical evidence. This study was aimed to develop and validate a questionnaire for predicting high salt intake based on salt usage using dietary behaviors that affect salt intake in cooperation with Niigata prefecture, Japan. The study was comprised of 760 participants aged 21 - 79 years (Men%: 49%) living in Niigata. Participants completed a questionnaire about dietary behaviors potentially relevant to salt intake. Second morning voiding urine for estimating dietary salt intake per day was collected on the same day. The relationship of estimated dietary salt intake to answers on the questionnaire was examined using analysis of covariance for age, gender, body mass index, and whether taking medication or not. Ten items on the questionnaire revealed an association with dietary salt intake per day: making oneself gorge on a meal, having ≥2 different staples per meal, having a 1-dish meal, number of simmered dishes per day, variety of pickled vegetables per day, having salt-cured fish eggs per day, quantity of soup consumed when eating noodles, preference for strong-tasting meals, frequency of eating out, and frequency of alcohol consumption.The current study identified 10 dietary behaviors that enabled the development of a salt intake questionnaire for identifying specific dietary behaviors for assessing regions and/or individuals when attempting to encourage salt reduction. 展开更多
关键词 SALT Intake Dietary Behavior Second MORNING VOIDING URINE URINARY Sodium Japanese
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SIMULTANEOUS DETERMINATION OF EPHEDRINE AND ITS ANALOGUES IN URINE BY CAPILLARY GAS CHROMATOGRAPHY
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作者 Lin LI Jian Fang CUI Kai Rong CUI Yi ZHOU Mu Zou WANG Tong Hui ZHOU (Institute of Materia Medica,Chinese Academy of Medical Sciences,Beijing 100050) 《Chinese Chemical Letters》 SCIE CAS CSCD 1990年第2期175-178,共4页
A method is developed for the simultaneous determination of ephedrine,pseudoephedrine, norephedrine, norpseudoephedrine and methylephedrine in urine on a capillary column using nitrogen-phosphorus detector.Diphenylami... A method is developed for the simultaneous determination of ephedrine,pseudoephedrine, norephedrine, norpseudoephedrine and methylephedrine in urine on a capillary column using nitrogen-phosphorus detector.Diphenylamine is used as the internal standard.Calibration graphs are linear down to 1.30ug/ml urine. 展开更多
关键词 GAS IV SIMULTANEOUS DETERMINATIon OF EPHEDRINE AND its ANALOGUES IN URINE BY CAPILLARY GAS CHROMATOGRAPHY its
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The effect of complete and incomplete Pelvic Autonomic nerves Preservation on Urination Function in Patient With Resection of Rectal Carcinoma
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作者 Shixin Xu Huaxiang Cao Ping. He 《Journal of Nanjing Medical University》 2006年第3期169-171,共3页
Objective: To evaluate the influence of complete and incomplete pelvic autonomic nerve preservation (PANP) on urination function in patients with resection of rectal carcinoma. Methods: The changes of pre-and post... Objective: To evaluate the influence of complete and incomplete pelvic autonomic nerve preservation (PANP) on urination function in patients with resection of rectal carcinoma. Methods: The changes of pre-and post-operative residual urine volume (RUV) in patient undergoing complete PANP (n = 15) and incomplete PANP (n = 17) were observed. Results: In fifteen cases with complete PANP, preoperative RUV was 4.09 ml, 14 days and 3 months after operation RUV were 8.00 ml and 7.02 ml (P 〉 0.05). In seventeen cases with incomplete PANP, preoperative RUV was 3.90 ml, 14 days and 3 months after operation RUV were 36.55 ml and 22.64 ml (P 〈 0.001 ). Conclusion: Complete and incomplete pelvic autonomic preservation may affect urination function in patients with resection of rectal carcinoma and RUV is an effective indicator if the pelvic autonomic nerves are preserved completely. 展开更多
关键词 Rectal carcinoma Pelvic autonomic nerve urination function
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Analysis of γ-Hydroxybutyrate in Human Urine by LC-MSIMS Method and Its Forensic Application
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作者 Luan Yujing Wang Ruihua Dong Ying 《Journal of Chemistry and Chemical Engineering》 2017年第3期87-89,共3页
GHB (γ-hydroxybutyrate) is becoming popular recreational drugs. As a result of its strong sedative and amnesiac effects, GHB has been implicated in a number of DFSA cases. The natural presence of GHB in the human b... GHB (γ-hydroxybutyrate) is becoming popular recreational drugs. As a result of its strong sedative and amnesiac effects, GHB has been implicated in a number of DFSA cases. The natural presence of GHB in the human body and its rapid elimination after ingestion make it difficult to detect and to evaluate its roles in suspected GHB-facilitated assaults. The paper describes an analytical method for the determination of GHB in urine using LC-MS/MS. Samples were acidified by ammonium chloride solution and extracted with ethyl acetate, and then the extracts were analysed by LC-MS/MS. The limit of detection was 0.05 p.g/mL (S/N = 3). The intra- and inter-day precision was within 10.0% at three concentrations. The methods were found to be sensitive, accurate, rapid and suitable for the forensic toxicology to test GHB in real cases. 展开更多
关键词 γ-Hydroxybutyrate (GHB) URINE LC/MS/MS forensic application.
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Expression of CD80, TNF-αand IL-4 in preeclampsia and its value on clinical prognosis
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作者 Li Wang Yi-Ling Ding 《Journal of Hainan Medical University》 2019年第23期40-44,共5页
Objective:We assessed the value of measuring the expression of CD80 and its correlations of TNF-α、and IL-4 in preeclampsia.Methods 30 patients with mild preeclampsia,27 patients with severe preeclampsia and 30 case ... Objective:We assessed the value of measuring the expression of CD80 and its correlations of TNF-α、and IL-4 in preeclampsia.Methods 30 patients with mild preeclampsia,27 patients with severe preeclampsia and 30 case of normal pregnancy were recruited.mRNA and protein expression levels of CD80,TNF-αand IL-4 were detected by qRT-PCR and ELISA.Result(1)The protein of CD80and TNF-αincreased significantly(P<0.05),while the expression level of IL-4 decreased significantly in mild and severe preeclampsia(P<0.05).(2).The mRNA of CD80and TNF-αincreased significantly in peripheral blood mononuclear cell of mild and severe preeclampsia,while the expression level of IL-4 decreased significantly.(3)There was a correlation between CD80 and TNF-αand IL-4(P<0.05).CD80 were positively correlated wi th TNF-a,but negatively with IL-4 levels.Conclusion This suggests that CD80 may cause the occurrence of preeclampsia by affecting the balance of Th1/Th2. 展开更多
关键词 URINE CD80 PREECLAMPSIA Progress INF-a IL-4
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Multivariate Analysis of Female Stress Urinary Incontinence and Establishment of a Prediction Model
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作者 Lei Li Lin Luo +2 位作者 Junnai Wang Ying Hong Jianfang Geng 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2024年第8期931-935,共5页
Stress urinary incontinence(SUI)is a symptom of uncontrolled urine outflow that affects millions of women worldwide[1].SUI is a significant healthcare issue that affects the quality of life of women across numerous do... Stress urinary incontinence(SUI)is a symptom of uncontrolled urine outflow that affects millions of women worldwide[1].SUI is a significant healthcare issue that affects the quality of life of women across numerous domains,including social activities,physical health,mental well-being,employment,and sexual life. 展开更多
关键词 URINE URINARY MULTIVARIATE
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Effect of mechanical ventilation on urine volume and expression of aquaporins in rabbits
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作者 Yan Sun Jiamin Yang +9 位作者 Shujing Zhang Yushan Gao Yixi Jin Min Wang Lei Ni Zhenyi Wang Pengfei Kang Yan Qiu Xiaolei Liu Yuhang Li 《Journal of Traditional Chinese Medical Sciences》 2017年第3期272-279,共8页
Objective:To explore biological mechanisms underlying the traditional Chinese medicine theory of 'lung governing the regulation of water passage' by observing effects of mechanical ventilation on urine volume ... Objective:To explore biological mechanisms underlying the traditional Chinese medicine theory of 'lung governing the regulation of water passage' by observing effects of mechanical ventilation on urine volume and related bioactive substances.Methods:A total of 16 male rabbits were randomly divided into control and lung expansion model groups (n =8 per group),which were examined during the following four respiratory intervention stages (10 minutes each):(1) free breathing without any respiratory intervention;(2) lung expansion induced by mechanical ventilator;(3) free breathing without ventilator intervention;(4) cutting of bilateral sympathetic,vagus,and depressor nerves with mechanical ventilation.Data for breathing rate,blood pressure,and urine drops were recorded with a Medlab data acquisition system.Levels of nitric oxide (NO),antidiuretic hormone (ADH),endothelin 1 (ET-1),angiotensin Ⅱ (AngⅡ),prostaglandin E2 (PGE2),and atrial natriuretic peptide (ANP) were detected in lung tissue,kidney tissue,and serum by enzyme-linked immunosorbent assay.Protein expression of aquaporins 1 and 2 (AQP1 and AQP2) was detected in kidney tissue by immunohistochemistry,while Aqp1 mRNA and Aqp2 mRNA levels in kidney tissue were detected by real-time quantitative polymerase chain reaction.Histopathological changes in lung and kidney tissues were examined by hematoxylin and eosin staining.Results:Compared with the control group,number of urine drops during the second and fourth stages is significantly decreased in the model group (P <.05).In addition,NO and PGE2 content in lung and kidney tissues was significantly decreased in the model group (P <.05).ANP content in model group lung and kidney tissues was significantly increased (P <.05),while ET-1 content in these tissues was significantly decreased (P <.05).AngⅡ content in kidney tissue was significantly decreased in the model group (P <.05),while serum AngⅡ content of the animals was significantly increased (P <.05).Expression of AQP1 and AQP2 proteins in kidney tissue was significantly higher in the model group than in the control group (P <.05).No obvious histopathological changes were observed in lung or kidney tissues of animals in either group.Conclusions:Mechanical ventilation reduced urine volume,increased expression of AQP1 and AQP2 in kidney tissue,and altered levels of NO,ADH,ET-1,AngⅡ,PGE2,and ANP in lung tissue,kidney tissue,and serum.These results provide an experimental basis for preliminary studies of mechanisms under pinning the theory of 'lung governing the regulation of water passage'. 展开更多
关键词 Mechanical ventilation Lung governing the regulation of water PASSAGE URINE volume AQUAPORIN-1 AQUAPORIN-2
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