Objective To further investigate the effects of ultraviolet (UV) irradiated urocanic acid (UCA) on T lymphocytes, the effects of cis UCA on T lymphocyte IL 10 secretion was studied Methods Four groups of normal...Objective To further investigate the effects of ultraviolet (UV) irradiated urocanic acid (UCA) on T lymphocytes, the effects of cis UCA on T lymphocyte IL 10 secretion was studied Methods Four groups of normal lymphocytes were cultured in RPMI 1640 containing 15% fetal calf serum Besides negative and phytohemagglutinin (PHA) positive control, the cis UCA and trans UCA groups were set The number of lymphocytes was 1 5×10 6 PHA was 0 05mg/ml in each group Both cis UCA and trans UCA were detected by 1 00, 0 75, 0 50 and 0 20mmol/L respective concentrations IL 10 was detected by ELISA method after 48 hours' culture IL 10 mRNA expression level was assayed by RT PCR Results The results indicated cis UCA could reduce IL 10 secreted by T lymphocytes much more than trans UCA (t=2 91, P<0 05) could It was also found that the concentrations of cis UCA were negatively correlated with IL 10 levels (r=-0 99, t r=9 23, P<0 01) The IL 10 mRNA expression of T lymphoctytes was inhibited by cis UCA Conclusion Our investigations revealed that cis UCA, a major UV receptor in the stratum corneum of epidermis, could inhibit T lymphoctes secreting IL 10 by suppressing IL 10 mRNA expression, finally affects the humoral immune response展开更多
文摘Objective To further investigate the effects of ultraviolet (UV) irradiated urocanic acid (UCA) on T lymphocytes, the effects of cis UCA on T lymphocyte IL 10 secretion was studied Methods Four groups of normal lymphocytes were cultured in RPMI 1640 containing 15% fetal calf serum Besides negative and phytohemagglutinin (PHA) positive control, the cis UCA and trans UCA groups were set The number of lymphocytes was 1 5×10 6 PHA was 0 05mg/ml in each group Both cis UCA and trans UCA were detected by 1 00, 0 75, 0 50 and 0 20mmol/L respective concentrations IL 10 was detected by ELISA method after 48 hours' culture IL 10 mRNA expression level was assayed by RT PCR Results The results indicated cis UCA could reduce IL 10 secreted by T lymphocytes much more than trans UCA (t=2 91, P<0 05) could It was also found that the concentrations of cis UCA were negatively correlated with IL 10 levels (r=-0 99, t r=9 23, P<0 01) The IL 10 mRNA expression of T lymphoctytes was inhibited by cis UCA Conclusion Our investigations revealed that cis UCA, a major UV receptor in the stratum corneum of epidermis, could inhibit T lymphoctes secreting IL 10 by suppressing IL 10 mRNA expression, finally affects the humoral immune response
