To research the hair-care efficacy of prinsepia utilis oil.We evaluated the haircare efficacies of prinsepia utilis oil through methods such as differential scanning calorimetry(DSC),measuring difficulty level and mec...To research the hair-care efficacy of prinsepia utilis oil.We evaluated the haircare efficacies of prinsepia utilis oil through methods such as differential scanning calorimetry(DSC),measuring difficulty level and mechanical resistance against combing of wet and dry hair,scanning electron microscopy(SEM).According to the analysis of differential scanning calorimetry(DSC),the testing sample has repairing effect on the hairα-keratin damage induced by heated blowing.According to the pictures of scanning electron microscopy(SEM),The test results indicate the repairing or resisting thermal damage effects induced by hair drier of prinsepia utilis oil.By comparison with the blank control group,the strength and work of dry comb and wet comb were significantly reduced in the samples tested with 2%prinsepia utilis oil concentration(P﹤0.05).This indicates the testing sample has the efficacy to improve the easiness of combing and anti-tangle.And friction work is significantly reduced This indicates the smoothing efficacy of the testing sample.and the hair scales status is better;this indicates hair scales repairing efficacy of testing sample.Conclusion:PRINSEPIA UTILIS OIL has the effect of repairing hair scales,resisting thermal damage caused by hair dryer,easy to comb,anti-tangle,smoothing.展开更多
According to the brief understanding of Multiple Intelligences Theory, the author states that the eight intelligences should be awaked and utilized in teaching and reality. The teacher's job is to observe his stud...According to the brief understanding of Multiple Intelligences Theory, the author states that the eight intelligences should be awaked and utilized in teaching and reality. The teacher's job is to observe his students, diagnose his students and offer a prescription for their educational problems through exerting all his multiple intelligences skills.展开更多
The biosorption capacity differences of copper cation by yeast Candida utilis cells and isolated cell walls were investigated. The results showed that the metal accumulated by cell walls was usually 50% higher than th...The biosorption capacity differences of copper cation by yeast Candida utilis cells and isolated cell walls were investigated. The results showed that the metal accumulated by cell walls was usually 50% higher than that by intact cells. This suggested that the cell walls of yeast were the main sites for heavy metal accumulation. Treatments of cell wall with proteinases demonstrated that the copper accumulated by trypsin-treated cell walls was almost the same as that by intact cell walls (95%), while the subtilisin-treated cell walls almost lost the potential of metal accumulateion (only 3%), suggesting that the main sites of copper accumulation in Candida utilis cell walls were trypsin-unsensitive integral proteins. Fig 1, Ref展开更多
A new hemiterpenoid, （＋）-（2R,3S）-2-chloro-3-hydroxy-3-methyl-T-buty-rolactone was isolatexi from the leaves of Prinsepia utilis Royle. Its structure was clucidatexi by spectroscopic methods and X-ray crystallogra...A new hemiterpenoid, （＋）-（2R,3S）-2-chloro-3-hydroxy-3-methyl-T-buty-rolactone was isolatexi from the leaves of Prinsepia utilis Royle. Its structure was clucidatexi by spectroscopic methods and X-ray crystallographic analysis.展开更多
The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11.The results of our researches...The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11.The results of our researches were obtained as follow : the cell suspending mixture obtained the highest enzyme activity(20.4U/mL),after being treated with by Triton X-100 and β-ME(5%,v/v) and ultrasonic.The enzyme activity obtained by this method was obviously higher than by single treating method,e.g.ultrasonic(7.6U/mL) or Triton X-100 and β-ME(15.2U/mL).展开更多
Under the optimal condition of copper ions adsorption on yeast,we found some different effects among static adsorption, shaking adsorption and negative pressure cavitation adsorption, and the methods of yeast with dif...Under the optimal condition of copper ions adsorption on yeast,we found some different effects among static adsorption, shaking adsorption and negative pressure cavitation adsorption, and the methods of yeast with different pretreatments also affect adsorption of copper ions. At the same time, the change of intercellular pH before and after adsorption of copper with BCECF was studied. The copper distribution was located by using PhenGreen （dipotassium salt and diacetate）, and the surface of yeast was observed by an atomic force microscope. The results showed that negative pressure cavitation can improve bioadsorption capacity of copper ions on yeast. However, the yeasts＇ pretreatment has a higher effect on bioadsorption. It indicates that heavy metal bioadsorption on yeast has much relation with its cellular molecule basis. With the adsorping, the intercellular pH of yeast increased gradually and changed from acidity to alkalescence. These results may suggest that negative pressure cavitation can compel heavy metals to transfer from the cell surface into inside cell and make the surface of yeast coarse.展开更多
The purpose of this study is to isolate the active fraction from the crude extract of Prinsepia utilis Royle and to determine its antibacterial activity against the standard and drug-resistant bacteria strains in vitr...The purpose of this study is to isolate the active fraction from the crude extract of Prinsepia utilis Royle and to determine its antibacterial activity against the standard and drug-resistant bacteria strains in vitro. The Prinsepia utilis Royle was extracted in three portions by using petroleum ether, ethanol and water by the continuation recirculate extraction. The antibacterial activity of the extracts were evaluated by the determination of Minimum Inhibitory Concentration （MIC） and Minimum Bactericidal Concentration （MBC） values. The results showed that the ethanol extract and aqueous extract of petroleum ether showed the weakest inhibition. MIC values of the aqueous Prinsepia utilis Royle were found that they had significant effects against bacteria, while ethanol extracts against three standard bacteria （S. aureus ATCC25923, E. coli 44102, Salmonella 50041） were 25, 25, 50 mg·mL^-1 and 50, 100, 100 mg·mL^-1, MIC values of three drug-resistant bacteria （S. aureus GL17, E. coli EYAC08-56, Salmonella STQD2G.） were 50, 50, 50 mg·mL^-1 and 100, 100, 200 mg·mL^-1, respectively. MBC values of two extracts against three standard strains were 25, 50, 100mg·mL^-1 and 50, 100, 200 mg·mL^-1, MBC values of three drug-resistant bacteria were 100, 100, 100mg·mL^-1 and 200, 200, 200 mg·mL^-1, respectively.展开更多
Seven fractions were isolated from the ethyl acetate extract of Prinsepia utilis Royle by silica gel column chromatography. The antibacterial activities determined by disc diffusion method in vitro, indicated that the...Seven fractions were isolated from the ethyl acetate extract of Prinsepia utilis Royle by silica gel column chromatography. The antibacterial activities determined by disc diffusion method in vitro, indicated that the first and fourth fractions showed better antibacterial activity than the other fractions, while the sixth and seventh fractions did not showed any antibacterial activity. The diameters of the inhibition zone of first and fourth fractions were greater than 10 mm against three standard strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC44102 and Salmonella sp. CMCC(B)50041) at the concentration of 20 mg/ml. The first fraction was then repeatedly recrystallized in acetone to yield a white snowflake-like compound A, the inhibition zones of which were 14.03 mm, 11.54 mm and 12 mm, respectively. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were measured by broth dilution method at the concentrations ranging from 20 to 0.313 mg/mL. The MIC and MBC values of the first, fourth fractions and compound A were lower than that of oregano oil (positive control) against S. aureus ATCC25923.展开更多
Betula utilis D.Don.is an important species of alpine Himalaya and forms the major treeline component of western Himalaya.The different populations of B.utilis are declining and are under high risk.In the present stud...Betula utilis D.Don.is an important species of alpine Himalaya and forms the major treeline component of western Himalaya.The different populations of B.utilis are declining and are under high risk.In the present study,novel expressed sequence tag-simple sequence repeat(EST-SSR)primers were developed from expressed sequence tag(EST)data of different Betula species.Of the10,796 designed primers,the percentages of di-,tri-,tetra-,penta-,and hexa-repeats were 36%,35%,15%,5.5%and7.7%,respectively.For validation,50 primers were synthesized randomly and were characterized in 20 different B.utilis accessions from north-western Himalaya.Of these,45 primers amplified fragments in a range of 1-6.The 24 polymorphic primers produced 111 fragments in aggregate with 4.6 fragments on average.Polymorphism information content(PIC)ranged from 0.288 in marker BUMS-24 to 0.497 in BUMS-3 and BUMS-7,with an average of 0.447 among polymorphic markers.Dendrogram based on Jaccard’s similarity coefficient and UPGMA method showed that newly developed SSR markers distinguished twenty accessions of B.utilis into two groups.As no SSR markers were available in this species,the newly developed markers will foster molecular genetics research and conservation efforts for this species.展开更多
产朊假丝酵母是生物安全(Generally Recognized as Safe,GRAS)的微生物,也是一种重要的工业微生物。近20年来,随着分子生物学技术的发展,产朊假丝酵母的基因表达系统和基因工程研究及开发应用取得了显著的进展,使得利用该菌表达多种物...产朊假丝酵母是生物安全(Generally Recognized as Safe,GRAS)的微生物,也是一种重要的工业微生物。近20年来,随着分子生物学技术的发展,产朊假丝酵母的基因表达系统和基因工程研究及开发应用取得了显著的进展,使得利用该菌表达多种物质成为可能。本文概述了产朊假丝酵母的生物学特点、外源基因表达系统、基因敲除、遗传转化等方面的研究和应用进展。展开更多
文摘To research the hair-care efficacy of prinsepia utilis oil.We evaluated the haircare efficacies of prinsepia utilis oil through methods such as differential scanning calorimetry(DSC),measuring difficulty level and mechanical resistance against combing of wet and dry hair,scanning electron microscopy(SEM).According to the analysis of differential scanning calorimetry(DSC),the testing sample has repairing effect on the hairα-keratin damage induced by heated blowing.According to the pictures of scanning electron microscopy(SEM),The test results indicate the repairing or resisting thermal damage effects induced by hair drier of prinsepia utilis oil.By comparison with the blank control group,the strength and work of dry comb and wet comb were significantly reduced in the samples tested with 2%prinsepia utilis oil concentration(P﹤0.05).This indicates the testing sample has the efficacy to improve the easiness of combing and anti-tangle.And friction work is significantly reduced This indicates the smoothing efficacy of the testing sample.and the hair scales status is better;this indicates hair scales repairing efficacy of testing sample.Conclusion:PRINSEPIA UTILIS OIL has the effect of repairing hair scales,resisting thermal damage caused by hair dryer,easy to comb,anti-tangle,smoothing.
文摘According to the brief understanding of Multiple Intelligences Theory, the author states that the eight intelligences should be awaked and utilized in teaching and reality. The teacher's job is to observe his students, diagnose his students and offer a prescription for their educational problems through exerting all his multiple intelligences skills.
文摘The biosorption capacity differences of copper cation by yeast Candida utilis cells and isolated cell walls were investigated. The results showed that the metal accumulated by cell walls was usually 50% higher than that by intact cells. This suggested that the cell walls of yeast were the main sites for heavy metal accumulation. Treatments of cell wall with proteinases demonstrated that the copper accumulated by trypsin-treated cell walls was almost the same as that by intact cell walls (95%), while the subtilisin-treated cell walls almost lost the potential of metal accumulateion (only 3%), suggesting that the main sites of copper accumulation in Candida utilis cell walls were trypsin-unsensitive integral proteins. Fig 1, Ref
文摘A new hemiterpenoid, （＋）-（2R,3S）-2-chloro-3-hydroxy-3-methyl-T-buty-rolactone was isolatexi from the leaves of Prinsepia utilis Royle. Its structure was clucidatexi by spectroscopic methods and X-ray crystallographic analysis.
文摘The combination of ultrasonic and Triton X-100 and β-ME made it easy to release Uricase from Torula utilis Henneb 2.281 quickly when the pH of cell suspending mixture varied from 7 to 11.The results of our researches were obtained as follow : the cell suspending mixture obtained the highest enzyme activity(20.4U/mL),after being treated with by Triton X-100 and β-ME(5%,v/v) and ultrasonic.The enzyme activity obtained by this method was obviously higher than by single treating method,e.g.ultrasonic(7.6U/mL) or Triton X-100 and β-ME(15.2U/mL).
基金The National Basic Research Program (973) of China (No. 2004CB418505)
文摘Under the optimal condition of copper ions adsorption on yeast,we found some different effects among static adsorption, shaking adsorption and negative pressure cavitation adsorption, and the methods of yeast with different pretreatments also affect adsorption of copper ions. At the same time, the change of intercellular pH before and after adsorption of copper with BCECF was studied. The copper distribution was located by using PhenGreen （dipotassium salt and diacetate）, and the surface of yeast was observed by an atomic force microscope. The results showed that negative pressure cavitation can improve bioadsorption capacity of copper ions on yeast. However, the yeasts＇ pretreatment has a higher effect on bioadsorption. It indicates that heavy metal bioadsorption on yeast has much relation with its cellular molecule basis. With the adsorping, the intercellular pH of yeast increased gradually and changed from acidity to alkalescence. These results may suggest that negative pressure cavitation can compel heavy metals to transfer from the cell surface into inside cell and make the surface of yeast coarse.
基金Supported by Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT0848)
文摘The purpose of this study is to isolate the active fraction from the crude extract of Prinsepia utilis Royle and to determine its antibacterial activity against the standard and drug-resistant bacteria strains in vitro. The Prinsepia utilis Royle was extracted in three portions by using petroleum ether, ethanol and water by the continuation recirculate extraction. The antibacterial activity of the extracts were evaluated by the determination of Minimum Inhibitory Concentration （MIC） and Minimum Bactericidal Concentration （MBC） values. The results showed that the ethanol extract and aqueous extract of petroleum ether showed the weakest inhibition. MIC values of the aqueous Prinsepia utilis Royle were found that they had significant effects against bacteria, while ethanol extracts against three standard bacteria （S. aureus ATCC25923, E. coli 44102, Salmonella 50041） were 25, 25, 50 mg·mL^-1 and 50, 100, 100 mg·mL^-1, MIC values of three drug-resistant bacteria （S. aureus GL17, E. coli EYAC08-56, Salmonella STQD2G.） were 50, 50, 50 mg·mL^-1 and 100, 100, 200 mg·mL^-1, respectively. MBC values of two extracts against three standard strains were 25, 50, 100mg·mL^-1 and 50, 100, 200 mg·mL^-1, MBC values of three drug-resistant bacteria were 100, 100, 100mg·mL^-1 and 200, 200, 200 mg·mL^-1, respectively.
文摘Seven fractions were isolated from the ethyl acetate extract of Prinsepia utilis Royle by silica gel column chromatography. The antibacterial activities determined by disc diffusion method in vitro, indicated that the first and fourth fractions showed better antibacterial activity than the other fractions, while the sixth and seventh fractions did not showed any antibacterial activity. The diameters of the inhibition zone of first and fourth fractions were greater than 10 mm against three standard strains (Staphylococcus aureus ATCC25923, Escherichia coli ATCC44102 and Salmonella sp. CMCC(B)50041) at the concentration of 20 mg/ml. The first fraction was then repeatedly recrystallized in acetone to yield a white snowflake-like compound A, the inhibition zones of which were 14.03 mm, 11.54 mm and 12 mm, respectively. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were measured by broth dilution method at the concentrations ranging from 20 to 0.313 mg/mL. The MIC and MBC values of the first, fourth fractions and compound A were lower than that of oregano oil (positive control) against S. aureus ATCC25923.
基金This work was nancially supported by DBT-IPLS scheme[Reference No.BT/PR4548/INF/22/146/2012].
文摘Betula utilis D.Don.is an important species of alpine Himalaya and forms the major treeline component of western Himalaya.The different populations of B.utilis are declining and are under high risk.In the present study,novel expressed sequence tag-simple sequence repeat(EST-SSR)primers were developed from expressed sequence tag(EST)data of different Betula species.Of the10,796 designed primers,the percentages of di-,tri-,tetra-,penta-,and hexa-repeats were 36%,35%,15%,5.5%and7.7%,respectively.For validation,50 primers were synthesized randomly and were characterized in 20 different B.utilis accessions from north-western Himalaya.Of these,45 primers amplified fragments in a range of 1-6.The 24 polymorphic primers produced 111 fragments in aggregate with 4.6 fragments on average.Polymorphism information content(PIC)ranged from 0.288 in marker BUMS-24 to 0.497 in BUMS-3 and BUMS-7,with an average of 0.447 among polymorphic markers.Dendrogram based on Jaccard’s similarity coefficient and UPGMA method showed that newly developed SSR markers distinguished twenty accessions of B.utilis into two groups.As no SSR markers were available in this species,the newly developed markers will foster molecular genetics research and conservation efforts for this species.
文摘产朊假丝酵母是生物安全(Generally Recognized as Safe,GRAS)的微生物,也是一种重要的工业微生物。近20年来,随着分子生物学技术的发展,产朊假丝酵母的基因表达系统和基因工程研究及开发应用取得了显著的进展,使得利用该菌表达多种物质成为可能。本文概述了产朊假丝酵母的生物学特点、外源基因表达系统、基因敲除、遗传转化等方面的研究和应用进展。
