Pathogenic bacterial contaminations in water cause serious or even lethal threats.Strategies that effectively kill bacteria without causing environmental contamination are urgently needed in a wide range of applicatio...Pathogenic bacterial contaminations in water cause serious or even lethal threats.Strategies that effectively kill bacteria without causing environmental contamination are urgently needed in a wide range of applications.We prepared recyclable antimicrobial magnetic nanoparticles,Fe304@P(St-coAcQAC),through surfactant-free seeded emulsion polymerization involving a polymerizable,hydrophobic quaternary ammonium compound(QAC).Fe304 particles were first synthesized by a solvothermal reaction,followed by functionalization with a methacrylic silane(MPS),and then copolymerized with a QAC-containing acrylic monomer(AcQAC),leading to Fe304@P(St-co-AcQAC) nanoparticles.As confirmed by antibacterial assays,these Fe304@P(St-co-AcQAC) nanoparticles exhibited strong antimicrobial action against both Gram-positive Staphylococcus epidermidis and Gram-negative Escherichia coli,without leaching out any bactericidal agent.An additional benefit of antimicrobial magnetic particles is that they can be easily recycled while maintaining excellent antimicrobial efficacy.展开更多
Antimicrobial resistance(AMR)has become a global health crisis in need of novel solutions.To this end,antibiotic combination therapies,which combine multiple antibiotics for treatment,have at-tracted significant atten...Antimicrobial resistance(AMR)has become a global health crisis in need of novel solutions.To this end,antibiotic combination therapies,which combine multiple antibiotics for treatment,have at-tracted significant attention as a potential approach for combating AMR.To facilitate advances in anti-biotic combination therapies,most notably in investigating antibiotic interactions and identifying synergistic antibiotic combinations however,there remains a need for automated high-throughput plat-forms that can create and examine antibiotic combinations on-demand,at scale,and with minimal reagent consumption.To address these challenges,we have developed a Robotic-Printed Combinatorial Droplet(RoboDrop)platform by integrating a programmable droplet microfuidic device that generates antibiotic combinations in nanoliter droplets in automation,a robotic arm that arranges the droplets in an array,and a camera that images the array of thousands of droplets in parallel.We further implement a resazurin-based bacterial viability assay to accelerate our antibiotic combination testing.As a demonstration,we use RoboDrop to corroborate two pairs of antibiotics with known interactions and subsequently identify a new synergistic combination of cefsulodin,penicillin,and oxacillin against a model E.coli strain.We therefore envision RoboDrop becoming a useful tool to efficiently identify new synergistic antibiotic combinations toward combating AMR.展开更多
基金the Distinguished Chair in Materials Science Endowment Fund at Georgia Southern University for the partial financial support of this research
文摘Pathogenic bacterial contaminations in water cause serious or even lethal threats.Strategies that effectively kill bacteria without causing environmental contamination are urgently needed in a wide range of applications.We prepared recyclable antimicrobial magnetic nanoparticles,Fe304@P(St-coAcQAC),through surfactant-free seeded emulsion polymerization involving a polymerizable,hydrophobic quaternary ammonium compound(QAC).Fe304 particles were first synthesized by a solvothermal reaction,followed by functionalization with a methacrylic silane(MPS),and then copolymerized with a QAC-containing acrylic monomer(AcQAC),leading to Fe304@P(St-co-AcQAC) nanoparticles.As confirmed by antibacterial assays,these Fe304@P(St-co-AcQAC) nanoparticles exhibited strong antimicrobial action against both Gram-positive Staphylococcus epidermidis and Gram-negative Escherichia coli,without leaching out any bactericidal agent.An additional benefit of antimicrobial magnetic particles is that they can be easily recycled while maintaining excellent antimicrobial efficacy.
基金Research reported in this publication was financially supported by the National Institutes of Health(R01AI117032,R01AI137272,and R01AI138978,USA).
文摘Antimicrobial resistance(AMR)has become a global health crisis in need of novel solutions.To this end,antibiotic combination therapies,which combine multiple antibiotics for treatment,have at-tracted significant attention as a potential approach for combating AMR.To facilitate advances in anti-biotic combination therapies,most notably in investigating antibiotic interactions and identifying synergistic antibiotic combinations however,there remains a need for automated high-throughput plat-forms that can create and examine antibiotic combinations on-demand,at scale,and with minimal reagent consumption.To address these challenges,we have developed a Robotic-Printed Combinatorial Droplet(RoboDrop)platform by integrating a programmable droplet microfuidic device that generates antibiotic combinations in nanoliter droplets in automation,a robotic arm that arranges the droplets in an array,and a camera that images the array of thousands of droplets in parallel.We further implement a resazurin-based bacterial viability assay to accelerate our antibiotic combination testing.As a demonstration,we use RoboDrop to corroborate two pairs of antibiotics with known interactions and subsequently identify a new synergistic combination of cefsulodin,penicillin,and oxacillin against a model E.coli strain.We therefore envision RoboDrop becoming a useful tool to efficiently identify new synergistic antibiotic combinations toward combating AMR.
