A Objective3 This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate （A/Chicken/ Hebei/WD/98, abbreviated as WD98） by comparing with other reference strains. I...A Objective3 This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate （A/Chicken/ Hebei/WD/98, abbreviated as WD98） by comparing with other reference strains. I-Method3 Eight complete genes were amplified by RT-PCR and sequenced. The homology and genetic evolution relationship were analyzed between these sequences and that of the seven reference strains. [Result] The whole genomic sequence of WD98 strain was 91.1% -95.8% homologous to that of seven reference strains tested. This isolate shared the highest homology （95.8%） to D/HK/Y280/97 and the lowest homology （91.1% ） to C/Pak/2/99. The HA cleavage site of the WD98 strain was R-S-S-R G, and the 226th amino acid at receptor-binding site was Gin. [ Condmion] WD98 strain belongs to mildly pathogenic avian in- fluenza virus and may not infect human. The genetic relationship is the closest between A/Chicken/Hebei/wD/98 and A/duck/HongKong/Y280/ 97, both of which belong to the sub-line of A/Chicken/Beijing/1/94 in Eurasian line. And A/Chicken/Hebei/WD/98 and A/Chicken/Beijing/1/94 are genetically distant within the same sub-line.展开更多
The Coefficient of Variation(CV)of hectometer yarn's weight is one of the guidelines to evaluate its intrinsic quality.In the spinning manufacturing,the control of cotton yarn's weight unevenness is accomplish...The Coefficient of Variation(CV)of hectometer yarn's weight is one of the guidelines to evaluate its intrinsic quality.In the spinning manufacturing,the control of cotton yarn's weight unevenness is accomplished mainly in terms of a spot-check on semi-product and a succedent adjust in process parameters during spinning based on technicians' experience.However,it is theoretically believed among manufacturers that with fixed technical levels and parameters in the spinning process,the quality parameters of assorted cotton have a certain influence on the CV.In order to find out a rule of the influence that assorted cotton has on the CV,a GM(1,N)model,correlated raw cotton's quality parameter with the CV,has firstly been developed according to the modeling theory of grey system,and then been applied in the designing step to predict the CV.It has been approved by practical modeling and validation that the model could fit preferably an accrual CV value,and provide a method of quantitative predicting analysis for textile manufacturers to design cotton yarn's quality.展开更多
To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber techniqu...To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber technique, from Sept 3, 2002 to Oct 30, 2003 in two types of soil ecosystems, of which one was covered with litters on the surface soil, and the other had no litters. The results showed that litters had significant influences on CO2, N2O and CH4 fluxes (p<0.05). Their diurnal change patterns of plot with litters and litter-free plot were similar, and they all showed emission/absorption peak at 18:00. The diurnal change fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the diurnal flux of CH4 of plot with litters was lower than that of litter-free plot. The fluxes of CO2, N2O, and CH4 showed the similar seasonal patternsfor both plots. The fluxes of CO2, CH4 showed their peak fluxes in June, but the fluxes of N2O showed its peak emissions in August. The annual fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the annual flux of CH4 of plot with litters was lower than that of litter-free plot. Keywords Flux - CO2, N2O and CH4 - Seasonal variation - Diurnal variation CLC number S718.5 Document code A Foundation item: The study was supported by innovation research project of Institute of Appiied Ecology, Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (30271068)Bigraphy: XIAO Dong-mei (1979-), female, master of Institute of Applied Ecology. Chinese Academy of Sciences, Shenyang 110016. P. R. China.Responsible editor: Song Funan展开更多
基金supported by subproject of National Program on Key Basic Research Project (973 Program )(2005CB523001)
文摘A Objective3 This study was to understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate （A/Chicken/ Hebei/WD/98, abbreviated as WD98） by comparing with other reference strains. I-Method3 Eight complete genes were amplified by RT-PCR and sequenced. The homology and genetic evolution relationship were analyzed between these sequences and that of the seven reference strains. [Result] The whole genomic sequence of WD98 strain was 91.1% -95.8% homologous to that of seven reference strains tested. This isolate shared the highest homology （95.8%） to D/HK/Y280/97 and the lowest homology （91.1% ） to C/Pak/2/99. The HA cleavage site of the WD98 strain was R-S-S-R G, and the 226th amino acid at receptor-binding site was Gin. [ Condmion] WD98 strain belongs to mildly pathogenic avian in- fluenza virus and may not infect human. The genetic relationship is the closest between A/Chicken/Hebei/wD/98 and A/duck/HongKong/Y280/ 97, both of which belong to the sub-line of A/Chicken/Beijing/1/94 in Eurasian line. And A/Chicken/Hebei/WD/98 and A/Chicken/Beijing/1/94 are genetically distant within the same sub-line.
基金Hunan Provincial Basic Science Foundation of China(No.2007FJ3046)Key Scientific Research Fundof Hunan Provincial Education Department,China(No.07A048)
文摘The Coefficient of Variation(CV)of hectometer yarn's weight is one of the guidelines to evaluate its intrinsic quality.In the spinning manufacturing,the control of cotton yarn's weight unevenness is accomplished mainly in terms of a spot-check on semi-product and a succedent adjust in process parameters during spinning based on technicians' experience.However,it is theoretically believed among manufacturers that with fixed technical levels and parameters in the spinning process,the quality parameters of assorted cotton have a certain influence on the CV.In order to find out a rule of the influence that assorted cotton has on the CV,a GM(1,N)model,correlated raw cotton's quality parameter with the CV,has firstly been developed according to the modeling theory of grey system,and then been applied in the designing step to predict the CV.It has been approved by practical modeling and validation that the model could fit preferably an accrual CV value,and provide a method of quantitative predicting analysis for textile manufacturers to design cotton yarn's quality.
基金The study was supported by innovation research project of Institute of Applied Ecology Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (302
文摘To understand influence of litters on the emission/absorption of CO2, N2O and CH4 in broadleaved/Korean pine forest in Changbai Mountain, fluxes of soil CO2, N2O and CH4 were measured by closed static chamber technique, from Sept 3, 2002 to Oct 30, 2003 in two types of soil ecosystems, of which one was covered with litters on the surface soil, and the other had no litters. The results showed that litters had significant influences on CO2, N2O and CH4 fluxes (p<0.05). Their diurnal change patterns of plot with litters and litter-free plot were similar, and they all showed emission/absorption peak at 18:00. The diurnal change fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the diurnal flux of CH4 of plot with litters was lower than that of litter-free plot. The fluxes of CO2, N2O, and CH4 showed the similar seasonal patternsfor both plots. The fluxes of CO2, CH4 showed their peak fluxes in June, but the fluxes of N2O showed its peak emissions in August. The annual fluxes of CO2 and N2O of plot with litters were significantly higher than those of the litter-free plot, while the annual flux of CH4 of plot with litters was lower than that of litter-free plot. Keywords Flux - CO2, N2O and CH4 - Seasonal variation - Diurnal variation CLC number S718.5 Document code A Foundation item: The study was supported by innovation research project of Institute of Appiied Ecology, Chinese Academy of Sciences (SCXZD0101-02) and National Natural Science Foundation of China (30271068)Bigraphy: XIAO Dong-mei (1979-), female, master of Institute of Applied Ecology. Chinese Academy of Sciences, Shenyang 110016. P. R. China.Responsible editor: Song Funan
