Boehmeria nivea var. strigosa (Urticaceae), a new variety from Southwest China

Boehmeria nivea var.strigosa Zeng Y.Wu&Y.Zhao,a new variety of B.nivea(Urticaceae)from Southwest China,is here described based on evidence from morphology and molecular phylogeny.This new variety is mainly characterized by its green abaxial leaf blade,partly connate stipules,and densely patent strigose hairs on stems and potioles.The phylogenetic analysis based on rbc L,nrDNA and rbc L+nrDNA datasets,revealed that all individuals of B.nivea var.strigosa formed a monophyletic group.The conservation status of B.nivea var.strigosa is assessed as“Near Threatened”(NT)according to IUCN evaluation criteria.The discovery of this new variety is not only crucial for the taxonomy of ramie,but also provides reference for the exploration and utilization of ramie.

<i>In-Vitro</i>Determination of Biological and Anabolic Functions of Weak Androgen Dehydroepiandrosterone (DHEA) Using a Variety of Cell Lines

Dehydroepiandrosterone (DHEA) is a weak androgen and is shown to have anti-cancer, anti-atherogenic, anti-adipogenic and anti-inflammatory effects on mouse, rat and rabbit models. However, human clinical trials data did not support animal findings and were inconclusive. These systemic differences in biological actions between rodents and humans were attributed to the low level of DHEA in rodents. In order to further understand the differences in biological functions between rodents and humans, we resorted to an in-vitroapproach involving mouse, rat and human cell lines to assess DHEA biological and anabolic functions separately and independently without systemic influence. Results indicated that DHEA was effective on mouse and rat cell lines but not on human cell lines, as observed in in-vivo studies. In addition, our in-vitrostudy showed that DHEA was able to induce myogenesis in mouse mesenchymal cells revealing its anabolic function, even though DHEA was considered as a weak androgen. This observation lent credence to the ban on DHEA by IOC medical commission, citing DHEA as an anabolic steroid. These in-vitro experiments suggested that the differences in biological actions of DHEA between rodents and humans existed not only in-vivo at the systemic level, but also in-vitro at the cellular level and thus paving the way to study the mechanism responsible for these differences at the cellular level itself.

LociScan,a tool for screening genetic marker combinations for plant variety discrimination

To reduce the cost and increase the efficiency of plant genetic marker fingerprinting for variety discrimination,it is desirable to identify the optimal marker combinations.We describe a marker combination screening model based on the genetic algorithm(GA)and implemented in a software tool,Loci Scan.Ratio-based variety discrimination power provided the largest optimization space among multiple fitness functions.Among GA parameters,an increase in population size and generation number enlarged optimization depth but also calculation workload.Exhaustive algorithm afforded the same optimization depth as GA but vastly increased calculation time.In comparison with two other software tools,Loci Scan accommodated missing data,reduced calculation time,and offered more fitness functions.In large datasets,the sample size of training data exerted the strongest influence on calculation time,whereas the marker size of training data showed no effect,and target marker number had limited effect on analysis speed.

Canine Myofascial Kinetic Lines: A Descriptive Dissection Study Including Related Function and Locomotion and Comparison of the Human and Equine Myofascial Kinetic Lines

Aim: This dissection study was conducted to verify if the Myofascial kinetic lines, outlined in detail in humans and recently documented in horses, were present in dogs. These dynamic lines present rows of interconnected muscles, myofascia and other fascia structures, which influence the biomechanics of the spine and limbs. Methods: Forty-two dogs of different breeds and genders were dissected, imaged, and videoed. Results: Similar kinetic lines were verified in the dog, as described in humans and horses, and additionally, three new branches of the lines were discovered. The kinetic lines described were three superficial lines: Dorsal, Ventral, and Lateral, which all started in the hindlimb and ended in the temporal and occipital regions. These lines act respectively in spinal extension, flexion, and lateral flexion. Three profound lines, which started in the tail and ended in the head. The Deep Dorsal Line followed the transversospinal myofascia. The Deep Ventral Line showed an additional start deep in the medial hind limb, continued in the hypaxial myofascia, and enveloped all the viscera. Also, the Deep Lateral Line started in the hindlimb but parted along the trunk in the deep lateral myofascial structures. Two helical lines crossed the midline two or three times and served to rotate the spine. The Functional Line established a sling from the axilla to the contralateral stifle and presented a new ipsilateral branch. The Spiral Line connected the head and the ipsilateral tarsus and additionally presented a new straight branch. The four front limb lines describe their motion: the Front Limb Protraction and Retraction, Adduction, and Abduction Lines. Conclusion: The canine lines mirrored the equine and human lines with exceptions due to differences in anatomy, foot posture, lumbosacral flexibility, and their biomechanical constitution as predator versus prey animals. Additionally, three new canine branches were verified and described.

Effect of Variety and Plant Spacing on Growth and Yield of Groundnuts (Arachis hypogaea L.)

A field experiment was carried out to determine the effect of variety and plant spacing on yield and growth of groundnuts. The field experiment was laid in a 3 × 3 factorial experiment in a Randomized Complete Block Design (RCBD) with three (3) replications. The factor A included three (3) groundnut varieties (Nkatie Sari, Sum Nutt 22 and Yenyawoso) and Factor B was the three (3) spacing of 30 cm × 15 cm, 30 cm × 30 cm and 30 cm × 40 cm. All recommended agronomic practices were followed. Data was collected from eight (8) tagged plants. Growth data were recorded on plant height, number of branches, number of leaves, and the number of flowers while yield data were collected on the number of flowers, number of pods per plant, 100 seeds weight and the pod yield (kg/ha). The plant spacing significantly influenced (P < 0.05) the growth and yield parameters. Groundnut grown at a spacing of 30 cm × 15 cm produced the maximum plant height, whereas the maximum number of leaves, number of branches and number of flowers were produced from 30 cm × 40 cm. Yenyawoso variety with a wider plant spacing performed better vegetatively among all the varieties. The Yenyawoso variety produced the highest number of pods, 100 seeds weight and pod yield as compared to the other varieties. Also, Yenyawoso at 30 cm × 40 cm spacing and Nkatie Sari at 30 cm × 15 cm spacing produced the maximum pod yield.

The Influence of Gratering, Fermentation and Variety on the Physio-Chemical Quality of Cassava Starch

The study was conducted to determine the influence of gratering and fermentation parameters on the physicochemical quality of starch obtained from two cassava varieties in Sierra Leone (i.e., SLICASS 11 and SLICASS 6). Fresh cassava roots harvested from the Department of Agricultural Engineering were peeled, washed and grated before fermentation and starch extraction. Fermentation was carried out under separate aerobic and anaerobic conditions for a period of Seven days. Physicochemical analyses were conducted at the Postharvest Food and Bioprocess Engineering Laboratory of the Department of Agricultural and Bio-System Engineering, Njala University to determine the solubility, water absorption capacity and swelling power of starch extracts obtained from various experimental treatments. Fermentation method and duration had significant effects on the solubility, water absorption capacity (WAC) and swelling power (SP) of starch extracts. Maximum solubility and WAC were recorded on the fifth day, for both cassava varieties tested, with apparent significant difference resulting from the two gratering bands (i.e., with 1.5-inch nail hole and 4-inch nail hole sizes, respectively). While swelling power increased consistently with temperature for starch obtained from SLICASS-11 variety, an irregular pattern was observed for SLICASS-6 variety. A multiple correlation analysis proposes a significant and weak correlation between temperature, WAC (+0.150) and swelling power (+0.048). Also multiple correlation analyses suggest a significant correlation between fermentation period, the functional properties of starch extracts obtained from both fermentation methods and cassava varieties (i.e., solubility (−0.226), water absorption capacity (+0.301) and swelling power (+0.329)).

Construction of apricot variety search engine based on deep learning

Apricot has a long history of cultivation and has many varieties and types. The traditional variety identification methods are timeconsuming and labor-consuming, posing grand challenges to apricot resource management. Tool development in this regard will help researchers quickly identify variety information. This study photographed apricot fruits outdoors and indoors and constructed a dataset that can precisely classify the fruits using a U-net model (F-score:99%), which helps to obtain the fruit's size, shape, and color features. Meanwhile, a variety search engine was constructed, which can search and identify variety from the database according to the above features. Besides, a mobile and web application (ApricotView) was developed, and the construction mode can be also applied to other varieties of fruit trees.Additionally, we have collected four difficult-to-identify seed datasets and used the VGG16 model for training, with an accuracy of 97%, which provided an important basis for ApricotView. To address the difficulties in data collection bottlenecking apricot phenomics research, we developed the first apricot database platform of its kind (ApricotDIAP, http://apricotdiap.com/) to accumulate, manage, and publicize scientific data of apricot.

Derivative Spectroscopy and its Application at Detecting the Weak Emission/Absorption Lines

The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line.

Characterization of the infectivity of an Indonesian Zika virus strain in mammalian cell lines

Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.

Variety Selection and Green Cultivation Technology of Courtyard Ornamental and Edible Peppers

In order to comply with the development trend of the multifunctional use of peppers,we conducted an investigation into the characteristics and features of varieties,potting management techniques,and the methods of extending the fruit ornamental period and other aspects of courtyard ornamental and edible peppers.A set of cultivation techniques suitable for courtyard ornamental and edible peppers has been developed,including timely sowing and seedling,nutrient soil preparation,water and fertilizer management,trimming and pruning,preservation of flowers and fruits,green prevention and control of diseases and pests,harvesting,and so on.

GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines

BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.

Co-variability Between the Broad Absorption Lines and Narrow Absorption Lines

We investigate the relationship between the variability of broad absorption lines(BALs)or narrow absorption lines(NALs)and that of continuum using a data set of two-epoch SDSS spectra containing 134 C IV NAL-BAL pairs.Our analysis reveals an anti-correlation between the fractional equivalent width(EW)variations in NALs(or BALs)and the fractional flux variations of the continuum,with Spearman rank correlation coefficients of r=-0.47(p=1E-08)and r=-0.58(p=1E-13),respectively.In addition,we find a positive correlation between the fractional EW variations in NALs and BALs(r=0.72,p=1E-22),and derive a regression equation ΔEW_(NAL)/<EW_(NAL)>=0.803ΔEW_(BAL)/<EW_(BAL)>+0.008,with an intrinsic scatter of 0.14.These results suggest that the variability in the ionizing continuum may play a significant role in the observed changes in C IV NALs and BALs,supporting the idea of photoionization-driven variability.The co-variability between C IV NALs and BALs may imply that they originate from outflows with similar physical conditions.

基于Hessian矩阵的改进EDlines输电线识别算法

随着电力网络的飞速发展,采用无人机搭载高清摄像头来进行输电线巡检已成常态。为提高巡检的实时性和准确性,本研究提出了一种基于Hessian矩阵的改进EDlines输电线识别算法。首先,通过伽马变换对输电线图像进行预处理,利用Hessian矩阵特征值和特征向量以求取像素点主方向和主曲率,并获得输电线主体轮廓,从而摒弃了传统方法中梯度计算锚点和像素方向的繁琐步骤。接着,在主体轮廓基础上连接锚点以得到潜在直线线段像素链,并运用随机抽样一致性(RANSAC)算法来进行线段拟合。最后,根据直线间的距离和角度,迭代拟合以得到最终的输电线。实验结果表明,该方法能应对多种复杂环境下的输电线识别任务,抗干扰能力强,误检率显著降低,为高空输电线巡检提供了可靠的技术支持,具有重要的工程应用价值。

New 4-imino-4H-Chromeno[2,3-d]Pyrimidin-3(5H)-Amine: Synthesis, Cytotoxic Effects on Tumoral Cell Lines and in Silico ADMET Properties

The synthesis of new 4-imino-4H-chromeno[2,3-d]pyrimidin-3(5H)-amine in four steps including one step under microwave dielectric heating is reported. The structural identity of the synthesized compounds was established according to their spectroscopic analysis, such as FT-IR, NMR and mass spectroscopy. These new compounds were tested for their antiproliferative activities on seven representative human tumoral cell lines (Huh7 D12, Caco2, MDA-MB231, MDA-MB468, HCT116, PC3 and MCF7) and also on fibroblasts. Among them, only the compounds 6c showed micromolar cytotoxic activity on tumor cell lines (1.8 50 50 > 25 μM). Finally, in silico ADMET studies ware performed to investigate the possibility of using of the identified compound 6c as potential anti-tumor compound.

Magnetic Field Curves and Magnetic Equipotential Surfaces around Crossing Electrical Wires Replacing Classical Magnetic Field Lines

This article is based on a recent model specifically defining magnetic field values around electrical wires. With this model, calculations of field around parallel wires were obtained. Now, this model is extended with the new concept of magnetic equipotential surface to magnetic field curves around crossing wires. Cases of single, double, and triple wires are described. Subsequent article will be conducted for more general scenarios where wires are neither infinite nor parallel.

Study on the Design Scheme of Vehicle Flow Lines in Unilateral Agglomerative Open Service Area

In recent years,with the introduction of the strategy to strengthen the country’s transportation infrastructure and the continuous implementation of new economic development models such as integrating transportation with tourism,various open service areas integrating highway service areas with local tourism have emerged nationwide.Examples include the Yangcheng Lake service area in Jiangsu and the Lengshui service area in Chongqing.This paper focuses on the design example of the Dazu Stone Carving service area on the Chongqing section of the Yurong Expressway,comprehensively considering factors such as construction scale,terrain conditions,and local urban planning.It analyzes and studies the traffic flow design of unilateral agglomerative open service areas around two vehicle traffic conversion links:between the main expressway and the service area,and between the service area and local roads.The aim is to provide a case study reference for similar projects.

Exploration and Practice of the“One Core,Two Lines,Four Dimensions”Ideological and Political Teaching Model:Taking the Interpretation of Concrete Plane Construction Drawings Course as an Example

This paper first deeply interprets the connotation of the“One Core,Two Lines,Four Dimensions”ideological and political teaching model in courses.Then,it explores the significant meaning of integrating this teaching model into the Interpretation of Concrete Plane Construction Drawings course.Finally,based on the challenges faced in this work,corresponding educational countermeasures are proposed to help improve the effectiveness and quality of ideological and political construction in the Interpretation of Concrete Plane Construction Drawings course and enhance the level of talent cultivation.

桃品种陇金5号栽培技术规程

甘肃省农业科学院林果花卉研究所针对甘肃省桃产业发展过程中对新品种的需求,通过多年的培育,选育出了品质优异的晚熟桃新品种陇金5号。从范围、规范性引用文件、术语和定义、品种来源、植物学特征、生物学特性、品质性状指标及栽培技术要点等方面对其规范,使其更好地服务于甘肃省桃产业发展。

诸城烟区烤烟品种(系)筛选试验

为筛选出适宜山东诸城烟区种植的烤烟新品种,以NC89作为常规对照,以当地主栽品种中烟100作为副对照,对QY2329、YY021和HB0901等9个品种(系)进行比较试验,测定其生育期、植物学性状、农艺性状、抗病性和经济性状。结果表明,11个品种(系)的生育期在130~140 d;植物学性状方面,HB0901田间生长不齐,其余品种(系)田间生长均为较齐或整齐,YY021、LY2804、CF241、HB056、龙江0110和LY716-2整个生育期长势强;农艺性状方面,各品种(系)株高118.5~162.8 cm,叶数20.0~29.3片,节距2.9~4.8 cm,茎围9.9~11.4 cm,腰叶长61.6~72.8 cm,腰叶宽30.1~39.8 cm;抗病性方面,各品种(系)主要发生病害为黑胫病、角斑/野火病和病毒病;经济性状方面,各品种(系)产量在2141.70~2795.10 kg/hm^(2),均高于对照NC89,其中HB0901、CF241、YM2020、龙江0110和LY716-2的产量高于副对照中烟100,CF241经济性状表现最好,均价31.32元,产值85672.73元/hm^(2),上中等烟比例达96.45%。综合田间生长状况及产值情况来看,CF241株型中等,生育期适中,分层落黄明显,田间长势及抗病性强,经济性状表现突出,综合表现最好;另外HB0901和龙江0110株型中等,分层落黄明显,抗病性强,经济性状表现优良;LY716-2株型高大,抗病性强且经济性状表现优良。综上,CF241、HB0901、龙江0110和LY716-2品种(系)适宜在诸城烟区进一步试种。

黑龙江省中晚熟地区旱直播水稻适宜品种筛选

为促进旱直播水稻新品种选育及推广应用,以黑龙江省第二、第三、第四积温带26个主栽水稻品种为试验材料,采用小区对比法研究供试品种生长发育特性、出苗情况、生育期、抗倒伏相关性状、产量及产量构成因素及品质差异情况,筛选适合黑龙江省中晚熟地区的旱直播品种。结果表明,绥粳25、绥粳306、龙庆稻8号、龙庆稻5号、龙粳66、龙粳31、绥粳27、绥粳103、绥粳18、龙粳1624和绥粳309出苗率高、出苗整齐。其中绥粳18、龙粳31、龙粳1624、绥粳27和龙庆稻5号,表现为抗倒伏性强、产量高、品质优良,另外还筛选出实际产量在7400 kg·hm^(-2)以上的品种包括绥粳106、三江6号、绥粳18、龙粳31、绥粳27、龙粳1624、龙庆稻8号、龙庆稻5号和绥粳309。综上,绥粳18、龙粳31、龙粳1624、绥粳27和龙庆稻5号可作为中晚熟地区的旱直播品种。