AIM： To elucidate the localization of vasodilator stimulated phosphoprotein （VASP）, a cytoskeletal organizing protein and a substrate of protein kinases A and G in mitotic gastric cancer cells. METHODS： Immunofluo...AIM： To elucidate the localization of vasodilator stimulated phosphoprotein （VASP）, a cytoskeletal organizing protein and a substrate of protein kinases A and G in mitotic gastric cancer cells. METHODS： Immunofluorescence microscopy was used to observe the localization of α-tubulin, VASP and Ser157 phosphorylated VASP （p-VASP） in interphase of mitotic gastric cancer of the cell line SGC-7901. RESULTS： Immunofluorescence staining showed that p-VASP but not VASP was co-localized with α-tubulin on spindle poles and fibers in prophase, metaphase and anaphase of the mitotic process of the gastric cancer cell line SGC-7901. H89, an inhibitor of protein kinases A and G, had no effect on the localization of p-VASP on the spindles. CONCLUSION： VASP may play a role in assembling and stabilizing the mitotic spindle of cells, and phosphorylation of the protein is the precondition for it to exert this function.展开更多
Aim:Extracellular matrix(ECM)-adhesions and their interaction with actin cytoskeleton are fundamental for hepatocellular carcinoma(HCC).Fascin-1,an actin-bundling protein,is correlated with poor HCC prognosis,and is k...Aim:Extracellular matrix(ECM)-adhesions and their interaction with actin cytoskeleton are fundamental for hepatocellular carcinoma(HCC).Fascin-1,an actin-bundling protein,is correlated with poor HCC prognosis,and is known regarding the molecular mechanism of its action.In this study,the authors investigated Fascin-1 basic molecular mechanism and cellular properties in HCC cells.Methods:Fascin-1 was silenced by small interfering RNA and the expression of actin.The ECM-adhesion-related proteins were assessed along with the cells’adhesion capacity in two cell lines that differ in terms of aggressiveness;the hepatoma cell line PLC/PRF/5(Alexander)and the highly invasive HCC cell line HepG2.Results:This study shows that Fascin-1 is upregulated in HepG2 cells compared to Alexander cells and when silenced leads to increased cell adhesion only in HepG2,while at the same time is associated with reduced migfilin and vasodilator-stimulated phosphoprotein(VASP)expression.Conclusion:This is the first study to show that Fascin-1 contributes to a more aggressive phenotype in HCC cells and acts through migfilin and VASP.展开更多
基金Supported by National Natural Science Foundation of China, No. 30340036 and 30470891 Startup Grant from Jiangsu University, and Grant of Zhenjiang Key Institute of Clinical Laboratory Medicine (SH2006066)
文摘AIM： To elucidate the localization of vasodilator stimulated phosphoprotein （VASP）, a cytoskeletal organizing protein and a substrate of protein kinases A and G in mitotic gastric cancer cells. METHODS： Immunofluorescence microscopy was used to observe the localization of α-tubulin, VASP and Ser157 phosphorylated VASP （p-VASP） in interphase of mitotic gastric cancer of the cell line SGC-7901. RESULTS： Immunofluorescence staining showed that p-VASP but not VASP was co-localized with α-tubulin on spindle poles and fibers in prophase, metaphase and anaphase of the mitotic process of the gastric cancer cell line SGC-7901. H89, an inhibitor of protein kinases A and G, had no effect on the localization of p-VASP on the spindles. CONCLUSION： VASP may play a role in assembling and stabilizing the mitotic spindle of cells, and phosphorylation of the protein is the precondition for it to exert this function.
基金supported by the European Association for the Study of the Liver Sheila Sherlock fellowship 2012.
文摘Aim:Extracellular matrix(ECM)-adhesions and their interaction with actin cytoskeleton are fundamental for hepatocellular carcinoma(HCC).Fascin-1,an actin-bundling protein,is correlated with poor HCC prognosis,and is known regarding the molecular mechanism of its action.In this study,the authors investigated Fascin-1 basic molecular mechanism and cellular properties in HCC cells.Methods:Fascin-1 was silenced by small interfering RNA and the expression of actin.The ECM-adhesion-related proteins were assessed along with the cells’adhesion capacity in two cell lines that differ in terms of aggressiveness;the hepatoma cell line PLC/PRF/5(Alexander)and the highly invasive HCC cell line HepG2.Results:This study shows that Fascin-1 is upregulated in HepG2 cells compared to Alexander cells and when silenced leads to increased cell adhesion only in HepG2,while at the same time is associated with reduced migfilin and vasodilator-stimulated phosphoprotein(VASP)expression.Conclusion:This is the first study to show that Fascin-1 contributes to a more aggressive phenotype in HCC cells and acts through migfilin and VASP.
