Near infrared spectroscopy （NIR） is now probably the most popular process analytical technology （PAT） for pharmaceutical and some other industries. However, unlike mid-IR, NIR is known to have difficulties in moni...Near infrared spectroscopy （NIR） is now probably the most popular process analytical technology （PAT） for pharmaceutical and some other industries. However, unlike mid-IR, NIR is known to have difficulties in monitoring crystallization or precipitation processes because the existence of solids could cause distortion of the spectra. This phenomenon, seen as unfavorable previously, is however an indication that NIR spectra contain rich information about both solids and liquids, giving the possibility of using the same instrument for multiple property characterization. In this study, transflectance NIR calibration data was obtained using solutions and slurries of varied solution concentration, particle size, solid concentration and temperature. The data was used to build calibration models for prediction of the multiple properties of both phases. Predictive models were developed for this challenging application using an approach that combines genetic algorithm （GA） and support vector machine （SVM）. GA is used for wavelength selection and SVM for mode building. The new GA-SVM approach is shown to outperform other methods including GA-PLS （partial least squares） and traditional SVM. NIR is thus successfully applied to monitoring seeded and unseeded cooling crystallization processes of L-glutamic acid.展开更多
N^(6)-methyldeoxyadenosine(6 mdA) modification is considered as a new epigenetic mark that may play important roles in various biological processes.However,it remains unclear about the effect of 6 mdA on DNA replicati...N^(6)-methyldeoxyadenosine(6 mdA) modification is considered as a new epigenetic mark that may play important roles in various biological processes.However,it remains unclear about the effect of 6 mdA on DNA replication in human cells.Herein,we combined next-generation sequencing with shuttle vector technology to explore how 6 mdA affects the efficiency and accuracy of DNA replication in human cells.Our results showed that 6 mdA neither blocked DNA replication nor induced mutations in human cells.Moreover,we found that the depletion of translesion synthesis DNA polymerase(Pol) κ,Pol η,Pol ι or Pol ζ did not significantly change the biological consequences of 6 mdA during replication in human cells.The negligible impact of 6 mdA on DNA replication is consistent with its potential role in epigenetic gene expression.展开更多
基金UK Engineering and Physical Sciences Research Council for funding the research (EPSRCGrant Reference: EP/C001788/1)
文摘Near infrared spectroscopy （NIR） is now probably the most popular process analytical technology （PAT） for pharmaceutical and some other industries. However, unlike mid-IR, NIR is known to have difficulties in monitoring crystallization or precipitation processes because the existence of solids could cause distortion of the spectra. This phenomenon, seen as unfavorable previously, is however an indication that NIR spectra contain rich information about both solids and liquids, giving the possibility of using the same instrument for multiple property characterization. In this study, transflectance NIR calibration data was obtained using solutions and slurries of varied solution concentration, particle size, solid concentration and temperature. The data was used to build calibration models for prediction of the multiple properties of both phases. Predictive models were developed for this challenging application using an approach that combines genetic algorithm （GA） and support vector machine （SVM）. GA is used for wavelength selection and SVM for mode building. The new GA-SVM approach is shown to outperform other methods including GA-PLS （partial least squares） and traditional SVM. NIR is thus successfully applied to monitoring seeded and unseeded cooling crystallization processes of L-glutamic acid.
基金supported by the National Natural Science Foundation of China (Nos. 21807030, 21907028)the Science and Technology Innovation Program of Hunan Province(No. 2019RS2020)+1 种基金Natural Science Foundation of Hunan Province(No. 2020JJ5046)the Fundamental Research Funds for the Central Universities (Nos. 531118010061, 531118010259)。
文摘N^(6)-methyldeoxyadenosine(6 mdA) modification is considered as a new epigenetic mark that may play important roles in various biological processes.However,it remains unclear about the effect of 6 mdA on DNA replication in human cells.Herein,we combined next-generation sequencing with shuttle vector technology to explore how 6 mdA affects the efficiency and accuracy of DNA replication in human cells.Our results showed that 6 mdA neither blocked DNA replication nor induced mutations in human cells.Moreover,we found that the depletion of translesion synthesis DNA polymerase(Pol) κ,Pol η,Pol ι or Pol ζ did not significantly change the biological consequences of 6 mdA during replication in human cells.The negligible impact of 6 mdA on DNA replication is consistent with its potential role in epigenetic gene expression.
