A possible alternative to synthetic agricultural chemicals is through the use of allelopathy. Adonis species are rich sources of secondary metabolites. Such allelochemicals offer potential for the development of futur...A possible alternative to synthetic agricultural chemicals is through the use of allelopathy. Adonis species are rich sources of secondary metabolites. Such allelochemicals offer potential for the development of future pesticides. Allelochemicals influence plant growth and cause morphological alterations. This visible effect could be due to primary effects at cellular or molecular level. Changes in the mitotic activity and disturbances in different phases of mitotic division are accepted as indicators of cytotoxic influence. Mitotic abnormalities and induction of micronuclei in interphase cells are parameters used to determine genotoxicity. The purpose of the current study was to establish the possible allelopathic effect of Adonis vernalis L. water extracts through evaluation of root growth inhibition effect and cytogenetic alterations. Adonis vernalis L. growing wild in Bulgaria was used in the present study. Two types of water extracts were prepared: Hot and Cold Water Extract of A. vernalis (HWЕА and СWЕА). A 72-h root growth inhibition test was provided in order to determine the toxicity level of extracts. EC50 values were determined. For toxicity test, seeds of Triticum aestivum L. cv. GTW were used. Cytotoxic and genotoxic potential of water extracts (EC50) were evaluated using Allium cepa L.-test. The EC50 for HWEA and CWEA was determined 1.83 g/l and 0.78 g/l respectively. Significant influence on mitotic activity values and a marked decrease in percentage of telophase cells were observed after treatment with both extracts. Adonis extracts also induced different mitotic abnormalities in root-tip cells of Allium cepa L. The percent of interphase cells with micronuclei increased significantly only after treatment with HWEA. The results indicated growth inhibitory, cytotoxic and genotoxic effects of crude water extracts of A. vernalis L. These effects demonstrated the presence of water soluble allelochemicals in Adonis aerial parts.展开更多
A. aegypti, the primary carrier for dengue viruses that cause dengue fever and are widespread over large areas of the tropics and subtropics. The copepod, Acanthocyclops vernalis was collected from a pond located in H...A. aegypti, the primary carrier for dengue viruses that cause dengue fever and are widespread over large areas of the tropics and subtropics. The copepod, Acanthocyclops vernalis was collected from a pond located in Hail, a city in northwestern Saudi Arabia. A. aegypti colonies established from Laboratory of Public Health Pests, Jeddah Municipality, Jeddah, Saudi Arabia. In the laboratory, the predatory capacity of copepod predator A. vernalis tested on first and second instars of Aedes aegypti. A single adult female of A. vernalis was tested against 50 larvae of A. aegypti in case the presence and absence of an alternative food. Several doses of A. vernalis (10, 15, 20 and 25 adults) against 100 larvae of mosquito were tested also in case the presence and absence of an alternative food under laboratory conditions.展开更多
文摘A possible alternative to synthetic agricultural chemicals is through the use of allelopathy. Adonis species are rich sources of secondary metabolites. Such allelochemicals offer potential for the development of future pesticides. Allelochemicals influence plant growth and cause morphological alterations. This visible effect could be due to primary effects at cellular or molecular level. Changes in the mitotic activity and disturbances in different phases of mitotic division are accepted as indicators of cytotoxic influence. Mitotic abnormalities and induction of micronuclei in interphase cells are parameters used to determine genotoxicity. The purpose of the current study was to establish the possible allelopathic effect of Adonis vernalis L. water extracts through evaluation of root growth inhibition effect and cytogenetic alterations. Adonis vernalis L. growing wild in Bulgaria was used in the present study. Two types of water extracts were prepared: Hot and Cold Water Extract of A. vernalis (HWЕА and СWЕА). A 72-h root growth inhibition test was provided in order to determine the toxicity level of extracts. EC50 values were determined. For toxicity test, seeds of Triticum aestivum L. cv. GTW were used. Cytotoxic and genotoxic potential of water extracts (EC50) were evaluated using Allium cepa L.-test. The EC50 for HWEA and CWEA was determined 1.83 g/l and 0.78 g/l respectively. Significant influence on mitotic activity values and a marked decrease in percentage of telophase cells were observed after treatment with both extracts. Adonis extracts also induced different mitotic abnormalities in root-tip cells of Allium cepa L. The percent of interphase cells with micronuclei increased significantly only after treatment with HWEA. The results indicated growth inhibitory, cytotoxic and genotoxic effects of crude water extracts of A. vernalis L. These effects demonstrated the presence of water soluble allelochemicals in Adonis aerial parts.
文摘A. aegypti, the primary carrier for dengue viruses that cause dengue fever and are widespread over large areas of the tropics and subtropics. The copepod, Acanthocyclops vernalis was collected from a pond located in Hail, a city in northwestern Saudi Arabia. A. aegypti colonies established from Laboratory of Public Health Pests, Jeddah Municipality, Jeddah, Saudi Arabia. In the laboratory, the predatory capacity of copepod predator A. vernalis tested on first and second instars of Aedes aegypti. A single adult female of A. vernalis was tested against 50 larvae of A. aegypti in case the presence and absence of an alternative food. Several doses of A. vernalis (10, 15, 20 and 25 adults) against 100 larvae of mosquito were tested also in case the presence and absence of an alternative food under laboratory conditions.
