The acute toxicity of Sodium dodecylbenzene sulfonate (SDBS) to subcamersed macrophyte Hydrilla verticillata (L.f) Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes: superoxide d...The acute toxicity of Sodium dodecylbenzene sulfonate (SDBS) to subcamersed macrophyte Hydrilla verticillata (L.f) Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes: superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), peroxidase (POD, EC 1.11.1.7) in the leaves were investigated under different concentrations of SDBS, The chlorophyll contents in leaves of experimental plants decreased gradually, while the electrical conductivity of culture solution increased gradually with the increased dose of SDBS. Under higher concentrations of SDBS (32 mg/L and 128 mg/L) treatment, SOD lost its activities completely after 24 h. The native PAGE analysis indicated the diversity of POD isoenzymes was obvious under different concentrations of SDBS stress.展开更多
Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were r...Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were randomly divided into (1) normal control group, (2) DSS group, (3) DSS + SASP treatment group, (4) DSS + PP treatment group. After the experiment, the mice in each group were observed daily general conditions, Immunohistochemistry was used to detect the expression of ERK, p38 MAPK and JNK protein in colonic mucosa of mice in each group. The expression of TNF-_and IL-6 cytokines in colonic tissue of mice was detected by ELASA. Results: The general situation of SASP group and PP group was better than DSS group;Compared with DSS group, the expression of ERK, p38 MAPK and JNK protein was significantly decreased. The levels of TNF-a and IL-6 in colon tissue of SASP group and PP group were significantly decreased. Conclusion: The results suggest that pectin polysaccharide from Rauvolfia hainanensis extract has a significant therapeutic effect on colitis in DSS mice, which may via regulating the MAPK signal pathway pathways.展开更多
基金the National Natural Science Foundation of China (39925007)the National High Technology Research and Development Program of China (863 Program) (2002AA601021)the Knowledge Innovation Program Key Project of Chinese Academy Sciences (KSCX2-SW-102)
文摘The acute toxicity of Sodium dodecylbenzene sulfonate (SDBS) to subcamersed macrophyte Hydrilla verticillata (L.f) Royle was studied. Chlorophyll contents and the activities of 3 antioxidant enzymes: superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), peroxidase (POD, EC 1.11.1.7) in the leaves were investigated under different concentrations of SDBS, The chlorophyll contents in leaves of experimental plants decreased gradually, while the electrical conductivity of culture solution increased gradually with the increased dose of SDBS. Under higher concentrations of SDBS (32 mg/L and 128 mg/L) treatment, SOD lost its activities completely after 24 h. The native PAGE analysis indicated the diversity of POD isoenzymes was obvious under different concentrations of SDBS stress.
基金National Natural Science Foundation of China(No.81360603)Natural Science Foundation of Hainan Province(No.813215).
文摘Objective: To investigate the effect and mechanism of Rauwolfia verticillata (Lour.) Baill. var. hainanensis Tsiang extract on mice with colitis induced by Dextran sulphate sodium (DSS). Methods: 80 BALB/C mice were randomly divided into (1) normal control group, (2) DSS group, (3) DSS + SASP treatment group, (4) DSS + PP treatment group. After the experiment, the mice in each group were observed daily general conditions, Immunohistochemistry was used to detect the expression of ERK, p38 MAPK and JNK protein in colonic mucosa of mice in each group. The expression of TNF-_and IL-6 cytokines in colonic tissue of mice was detected by ELASA. Results: The general situation of SASP group and PP group was better than DSS group;Compared with DSS group, the expression of ERK, p38 MAPK and JNK protein was significantly decreased. The levels of TNF-a and IL-6 in colon tissue of SASP group and PP group were significantly decreased. Conclusion: The results suggest that pectin polysaccharide from Rauvolfia hainanensis extract has a significant therapeutic effect on colitis in DSS mice, which may via regulating the MAPK signal pathway pathways.