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Study on Tourism Development and Community Participation in Villages of the Minorities ——With the Dong Village in Zhaoxing,Southeast Guizhou Autonomous Prefecture of Miao and Dong Nationalities,China
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作者 徐燕 陆仙梅 +2 位作者 吴再英 陈洪智 石贤昌 《Journal of Landscape Research》 2011年第3期85-89,共5页
Based on basic theories of community participation in tourism development,questionnaires were designed for the field investigation in one of the largest Dong Villages in China,the Dong village in Zhaoxing.Community pa... Based on basic theories of community participation in tourism development,questionnaires were designed for the field investigation in one of the largest Dong Villages in China,the Dong village in Zhaoxing.Community participation in tourism development of the Dong Village was analyzed from the perspectives of residents' participation in decision-making and management,reception and catering services,group performance,making and selling ethnic artworks,protection of village tourist resources and environment.And meanwhile,problems were pointed out as "still maintaining in the early stage of its tourism development,fewer opportunities for its residents participating in tourism development,resource protection being divorced from tourism benefits and so on",on the basis of which corresponding solutions were offered as "expanding the field of participation,improving the level of participation,increasing benefits of local residents;insisting on the principle of "not participating intentionally is the best way of their participation,guaranteeing economic compensation for rural residents;enhancing infrastructure construction;establishing a museum of folk-custom materials;creating a favorable atmosphere of folk cultures;enhancing cooperation with its neighboring villages in tourism development,and creating a "local industrial chain";establishing a research center of cultural tourism development of the Dong Minority. 展开更多
关键词 village of the MINORITIEs Rural tourism development community participation village of the Dong MINORITY in Zhaoxing
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Community Tourism in Changxi Village:Research based on Community-participated Village Tourism 被引量:4
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作者 LEI Tianlai YAN Tinggan 《Journal of Landscape Research》 2013年第6期46-48,52,共4页
On the basis of investigating community participation in tourism development of Changxi Village,Wuyuan County,the study pictures a reasonable image for the community participation in tourism village,proposes the probl... On the basis of investigating community participation in tourism development of Changxi Village,Wuyuan County,the study pictures a reasonable image for the community participation in tourism village,proposes the problems and corresponding suggestions. 展开更多
关键词 TOURIsM community PARTICIPATION Changxi village
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Community Tourism as Practiced in the Mountainous Qiang Region of Sichuan Province,China-a Case Study in Zhenghe Village 被引量:2
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作者 ZHOU Lianbin LIU Kaibang 《Journal of Mountain Science》 SCIE CSCD 2008年第2期140-156,共17页
In China, community tourism is still a relatively new phenomenon, but the villagers of a small Qiang village in the Qiang Autonomous County of Beichuan in Southwestern Sichuan have initiated tourism in a way which con... In China, community tourism is still a relatively new phenomenon, but the villagers of a small Qiang village in the Qiang Autonomous County of Beichuan in Southwestern Sichuan have initiated tourism in a way which conforms to the basic theory of community tourism development. This demonstrates that community tourism possesses a strength and vitality that can promote the development of tourism in the rural and mountainous areas. In the district of Zhenghe Village, the tourism industry, based on the community tourism model, is the mainstay of its economy. The practice of community tourism in the village not only promotes the economic development of the village community, but also leads to the protection of the mountainous natural environment and of the culture of the Qiang people. This paper investigates the development process of community tourism in Zhenghe and shows how the local residents participate in this process. It also looks at how profits have been distributed within the community. It demonstrates that community tourism is a correct choice by the Zhenghe people as they have dearly been moving from poverty to prosperity, while the local ecology and environment have been simultaneously protected. The authors hope that other minority villages with similar local conditions and natural resources will be able to use this example to develop their own community tourism. 展开更多
关键词 community protection Qiang minority sichuan China tourism environmental region Zhenghe village
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The Community Renewal of Shantytown Transformation in Old Industrial Cities:Evidence from Tiexi Worker Village in Shenyang,China 被引量:1
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作者 DONG Lijing WANG Yongchao +1 位作者 LIN Jiayi ZHU Ermeng 《Chinese Geographical Science》 SCIE CSCD 2020年第6期1022-1038,共17页
There are many problems,such as poverty,unemployment,poor infrastructure and how to improve the ecological environment,in the shantytowns of old industrial cities.Shantytowns are the most centralized areas with differ... There are many problems,such as poverty,unemployment,poor infrastructure and how to improve the ecological environment,in the shantytowns of old industrial cities.Shantytowns are the most centralized areas with different contradictions,referred to as the‘problem areas’of urban sustainable development.The shantytown transformation of old industrial cities is a typical process of unit community disintegration,which is reflected not only in the reconstruction of physical space but also in the community renewal of social spatial integration and culture reconstruction.Based on qualitative research,questionnaires and in-depth interviews,taking Tiexi Worker Village in Shenyang as a research case,this paper attempts to analyse the characteristics and driving forces of the community renewal of shantytown transformation in old industrial cities.We found that the physical space of Tiexi Worker Village has changed considerably,which is embodied in its land use structure,living environment and community service facilities,reflecting the development of community function from simple industrial function to comprehensive functions.The residents in this community have experienced a transformation from a period of homogeneity to one of heterogeneity.The social network of the community has been destroyed.Social stratification,social differentiation and higher fluidity have occurred.Community renewal is mainly affected by macro factors such as policy regulation,economic driving,condition constraints,and micro factors such as residents’choice of living space and willingness to renew their communities.The transformation policy of the old industrial zone and the development policy of the new urban area are the fundamental and deep-seated reasons for the renewal of Tiexi Worker Village,which determine the direction of the renewal and development of the community.The paid use of land and the development of the real estate industry are the direct stimulating factors for the renewal of Tiexi Worker Village,which become the direct reasons for the spatial transformation.The changes in the population in urban and rural areas and the promotion of traffic are the driving factors for the renewal of the community,while the choice of residents and their willingness regarding community renewal are the endogenous forces for promoting community renewal.Finally,the author attempts to put forward a model of the interlaced mechanism of the forming of community renewal at the macroscopic and microcosmic levels,which are the urban renewal and reconstruction and social space differentiation of community residents,respectively. 展开更多
关键词 old industrial city sHANTYTOWN community renewal mechanism Tiexi Worker village
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Community Renewal Strategy for Improving Community Resilience:A Case Study of River Ring Area of Caoyang New Village in Shanghai
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作者 WANG Zhenbao ZHU Lin +1 位作者 HAO Shiyang FAN Yuting 《Journal of Landscape Research》 2021年第4期29-31,36,共4页
In a new round of urban renewal,China’s urban space expansion is shifting from incremental development to inventory mining.Residents’ demands for improvement of community material environment and community cultural ... In a new round of urban renewal,China’s urban space expansion is shifting from incremental development to inventory mining.Residents’ demands for improvement of community material environment and community cultural identity are increasing.Meantime,affected by the COVID-19 pandemic in 2019,community resilience has become an urgent problem to be solved in urban communities.Based on resilience theory,TOD theory and other planning concepts,the paper analyzes the vulnerability of Shanghai Caoyang river ring area,and puts forward a multi-level community resilience improvement strategy,including increasing diversified public service facilities,building public pedestrian network,and reshaping public open space to improve the stability,adaptability and resilience of the community,in order to build the development path of resilient communities.The study will provide inspiration for future micro-renewal of communities and promote the sustainable development of urban communities. 展开更多
关键词 community resilience community renewal Caoyang New village Waterfront space Reform strategy
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Shiyou Village the Last Community of Han People
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《China & The World Cultural Exchange》 2001年第6期34-35,共2页
关键词 shiyou village the Last community of Han People
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Spatiotemporal Distributions of Bacterioplankton Communities in the Qiantang River(Hangzhou Section),China
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作者 XU Jiaojiao ZHAO Li +4 位作者 LUKWAMBE Betina NICHOLAUS Regan ZHU Jinyong YANG Wen ZHENG Zhongming 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第4期1102-1114,共13页
The spatial and temporal distribution of bacterioplankton communities plays a vital role in understanding the ecological dynamics and health of aquatic ecosystems.In this study,we conducted a comprehensive investigati... The spatial and temporal distribution of bacterioplankton communities plays a vital role in understanding the ecological dynamics and health of aquatic ecosystems.In this study,we conducted a comprehensive investigation of the bacterioplankton communities in the Qiantang River(Hangzhou section).Water samples were collected quarterly from seven sites over a one-year period.Physical and chemical parameters,including dissolved oxygen(DO),water temperature(WT),chemical oxygen demand(COD),nitrite(NO_(2)^(-)),active phosphate(PO_(4)^(3-))and other indices were determined.In this study,theαdiversity,βdiversity and abundance differences of bacterial communities were investigated using 16S rRNA high-throughput sequencing analysis.The spatial and temporal distribution characteristics and main driving factors of the bacterioplankton community structure and diversity were discussed.The results showed that a total of 57 phyla were detected in the bacterioplankton community,among which Proteobacteria and Actinomycetes were the two dominant groups with the highest relative abundance.The results of PCoA based on Bray-Curtis distance showed that the sampling season had a slightly greater effect on the changes in bacterioplankton community structure in the Qiantang River.Mantel and partial Mantel test showed that environmental variables(Mantel r=0.6739,P<0.0001;partial Mantel r=0.507,P=0.0001)were more important than geographical distance(Mantel r=0.5322,P<0.001;partial Mantel r=0.1563,P=0.001).The distance attenuation model showed that there was significant distance attenuation in all four seasons,and the maximum limit of bacterial community diffusion was found in spring.RDA analysis showed that nine environmental factors,including COD,WT and DO,significantly affected community distribution(P<0.05).This study provides valuable insights into the spatial and temporal distribution characteristics of bacterioplankton communities,shedding light on their ecological roles in the Qiantang River.The information obtained can guide future research on the interactions between bacterioplankton and their environment,enabling the development of effective conservation strategies and sustainable management practices for aquatic ecosystems. 展开更多
关键词 Qiantang River bacterioplankton community spatial and temporal distribution 16s rRNA range attenuation
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Knowledge Level about HIV/AIDS among the Villagers of Comilla District, Bangladesh 被引量:1
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作者 Md. Shafiur Rahman Md. Nuruzzaman Khan +3 位作者 Md. Nazrul Islam Mondal Md. Shamsher Alam Kazi Mohiuddin Ahmed Md. Rafiqul Islam 《World Journal of AIDS》 2014年第4期438-445,共8页
The HIV/AIDS epidemic has grown on an unprecedented scale since it was first recognized, and presently it is considered as a well known global public health crisis. In making the health policy where millions of popula... The HIV/AIDS epidemic has grown on an unprecedented scale since it was first recognized, and presently it is considered as a well known global public health crisis. In making the health policy where millions of populations are at risk, it is necessary to understand the knowledge level about HIV/AIDS. So, this study aimed at to investigate the knowledge level about HIV/AIDS among the villagers. Data and necessary information were collected from one hundred villagers of Salmanpur from Comilla district, Bangladesh through a structured questionnaire. The collected data were used for univariate analysis, to carry out the description of the variables;and to justify the total score of knowledge about HIV/AIDS among the respondents, Cronbach’s alpha (α) was calculated. The results revealed that almost all the respondents (92.00%) were ever heard about HIV/AIDS and half of them (51.00%) were known it as a communicable disease. Majority of the respondents (62.00%) believed that it is caused by heterosexual relation as well as sex with HIV positive persons. However, a good number of respondents were found unaware about the major causes and preventive ways of AIDS. Less than half of the respondents (45.00%) had medium knowledge (α = 0.34 - 0.66) and only 36.00% had the enough knowledge about HIV/AIDS. The knowledge level is increasing with the increase of education level, and the respondents who involved in agricultural works are found the lowest knowledge level (47.00%) about HIV/AIDS. The villagers were known about HIV/AIDS but they do not have accurate information about the ways in which AIDS virus can or cannot be transmitted. Therefore, effective programs should be taken to increase the knowledge level about HIV/AIDS among the villagers in Bangladesh. 展开更多
关键词 AIDs villageRs sexually Transmitted DIsEAsEs Cronbach’s ALPHA BANGLADEsH
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Architectural Culture of Hakka Walled Villages in Gannan: A Case Study of Yanyi Wei
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作者 WANG Zhongqing LAI Shihao 《Journal of Landscape Research》 2017年第6期94-98,102,共6页
This paper took Yanyi Wei for example to explore the architectural culture of Hakka walled vil ages in Gannan, including clan culture, defense culture, sacrificial culture, and feng shui culture. It pointed out that t... This paper took Yanyi Wei for example to explore the architectural culture of Hakka walled vil ages in Gannan, including clan culture, defense culture, sacrificial culture, and feng shui culture. It pointed out that there exist problems in Yanyi Wei, such as the disharmony between newly established houses and original buildings, the lack of proper maintenance, and the lack of heirs of walled village culture, and proposed reasonable suggestions about architectural conservation and cultural heritage. 展开更多
关键词 Hakka community in Gannan Architectural culture of walled villages Yanyi Wei Cultural heritage
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Comparative Analysis between Community and Occupants’ Rating Systems for Sustainable Urban Communities (SUC)
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作者 Mianda Khattab Salah El Haggar Ahmed El Gendy 《Journal of Environmental Protection》 CAS 2022年第11期881-894,共14页
Overpopulation globally is an addressed issue impacting human lives, marine lives, and the surrounding ecosystem;it is adding pressure on the available resources that should be optimized to suit the needs. Yet with im... Overpopulation globally is an addressed issue impacting human lives, marine lives, and the surrounding ecosystem;it is adding pressure on the available resources that should be optimized to suit the needs. Yet with improper management of resources and monitoring of daily activities, the environment will be further negatively impacted. With overpopulation higher urbanization rates are noticed with the demand of seeking better health facilities, better education, better jobs and better well-being;this progression is driving more demand into the infrastructure sector to be able to accommodate the growth rates. Hence, the need to having sustainable communities aiming at optimizing the resources used, working towards more feasible, environmentally friendly and cost-effective communities with a better occupant’s experience is in action. Sustainable development goals (SDG) are vital goals developed by the United Nations Development Program (UNDP) in 2015 to address and guide through 17 interconnected global goals serving the previously mentioned trend. Out of the 17 goals, Sustainable Cities and Communities (goal #11) and Good Health and Well-Being (goal #3) are the focus of this paper directed towards holding a comparative analysis between the community scale commonly known and mostly used rating system Leadership of Energy and Environmental Design (LEED-Cities and Communities) (USA) versus similar rating systems like Tarsheed-Communities (Egypt) and Estidama-Pearl (UAE) rating systems meeting sustainable development goal #11. Conjointly, another complimenting comparative review of the occupant’s health and wellbeing rating systems, such as Fitwel (USA) and Well (USA) are studied under sustainable development goal #3;however, they are focused on a building scale assessment. Living Community Challenge (LCC, USA) rating system linking community rating system with health & wellbeing credits was first issued in 2006, yet is it not cost effective neither easy to apply acting as a primary step while being affordable, accessible, and easy to implement. The objective of this paper is to highlight the pros and gaps under both categories of studies of community rating system and occupants’ health & wellbeing rating systems based on scientific content and commercial acceptance and do-ability. This comparison is done via comparing credits and sections within each rating system type;this will support in addressing the focal points needed for an integrated rating system between both categories that will serve in meeting SDG Sustainable Cities and Communities (goal #11) and Good Health and Well-Being (goal #3). 展开更多
关键词 sustainable Communities sustainable community Rating systems Occupant’s Health & Well-Being Rating system sDG#3 (Good Health and Well-Being) sDG#11 (sustainable Cities and Communities)
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Zhongguan Village,China's Silicon Valley
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作者 Liu Xinwen 《China's Foreign Trade》 2008年第14期000-000,共2页
In 1999,driven by the dream of using technology to change people's lives, Li Yanhong,returned to Zhongguan- cun (Zhongguan Village in Chinese), Beijing from Silicon Valley in the U.S.to create Baidu.com.Over the y... In 1999,driven by the dream of using technology to change people's lives, Li Yanhong,returned to Zhongguan- cun (Zhongguan Village in Chinese), Beijing from Silicon Valley in the U.S.to create Baidu.com.Over the years,Baidu has become the most frequently hitted website in China as well as the largest Chinese search engine and Chinese lan- guage website in the world.In 展开更多
关键词 Zhongguan village China’s silicon Valley CAs
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"Emperor's Power Far Away from the Countryside"and"Town in Charge of Village Affairs":The Extreme Grass-roots Management System is Not Conducive to the Healthy Development of the Countryside
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作者 Xiang CHEN 《Asian Agricultural Research》 2022年第8期13-14,共2页
This paper compares the two different rural management methods of"emperor’s power far away from the countryside"and"town in charge of village affairs",which shows that the extreme grass-roots mana... This paper compares the two different rural management methods of"emperor’s power far away from the countryside"and"town in charge of village affairs",which shows that the extreme grass-roots management system is not conducive to rural development.This paper also points out that rural development needs to find a road of sustainable development in line with its own characteristics,which is the fundamental shortcut to change poverty and become rich for a long time. 展开更多
关键词 "Emperor’s power far away from the countryside" "Town in charge of village affairs" Grass-roots management Rural management
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It's a Village Life!
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《China Today》 2002年第9期48-51,共4页
I love Beijing, but after a long working week, nothing suits me better than "heading for the hills," or really the village! We have found a quiet place to while away the weekend...no telephone, no computer, ... I love Beijing, but after a long working week, nothing suits me better than "heading for the hills," or really the village! We have found a quiet place to while away the weekend...no telephone, no computer, no honking horns and busy weekend shopping trips, no entertaining or being entertained. Friday afternoon ends and we are anxious to be out of the city as soon as possible. 展开更多
关键词 It’s a village Life
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HANDICRAFTS BOOST VILLAGER’S INCOME IN MAINLING
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作者 Palden Nyima 《China's Tibet》 2017年第6期29-31,共3页
Some Tibetan people regard it as a luxury or of high class to drink tea or wine in a silver-plated wooden bowl,with such bowls mostly made in Tibet’s places of lower attitude like in eastern Nyingchi City.Known as th... Some Tibetan people regard it as a luxury or of high class to drink tea or wine in a silver-plated wooden bowl,with such bowls mostly made in Tibet’s places of lower attitude like in eastern Nyingchi City.Known as the Tibetan medicinal world,the region’s Mainling 展开更多
关键词 BOOsT HANDICRAFTs BOOsT villageR’s INCOME IN MAINLING
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An Ancient Village in Beijing's Suburbs
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《China Today》 2000年第8期14-19,共6页
关键词 An Ancient village in Beijing’s suburbs
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Advancing Arts One village’s oil paintings lead the development of south China’s cultural industries
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作者 Zheng Yang 《ChinAfrica》 2013年第8期52-53,共2页
The Dafen art museum, the largest such museum in Shenzhen, was built int he village in 2007 to he a platform for art exhibitions and exchange,
关键词 Advancing Arts One village’s oil paintings lead the development of south China’s cultural industries
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Changes of Bacterial Community Structure in Copper Mine Tailings After Colonization of Reed (Phragmites communis) 被引量:10
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作者 CHEN Yu-Qing REN Guan-Ju +3 位作者 AN Shu-Qing SUN Qing-Ye LIU Chang-Hong SHUANG Jing-Lei 《Pedosphere》 SCIE CAS CSCD 2008年第6期731-740,共10页
Soil samples were collected from both bare and vegetated mine railings to study the changes in bacterial communities and soil chemical properties of copper mine tailings due to reed (Phragmites communis) colonizatio... Soil samples were collected from both bare and vegetated mine railings to study the changes in bacterial communities and soil chemical properties of copper mine tailings due to reed (Phragmites communis) colonization. The structures of bacterial communities were investigated using culture-independent 16S rRNA gene sequencing method. The bacterial diversity in the bare mine tailing was lower than that of the vegetated mine tailing. The former was dominated by sulfur metabolizing bacteria, whereas the latter was by nitrogen fixing bacteria. The bare mine tailing was acidic (pH = 3.78), whereas the vegetated mine tailing was near neutral (pH : 7.28). The contents of organic matter, total nitrogen, and ammonium acetate-extractable potassium in vegetated mine tailings were significantly higher than those in the bare mine railings (P 〈 0.01), whereas available phosphorus and electrical conductivity were significantly lower than those in the bare mine tailings (P 〈 0.01). The results demonstrated that 16S rRNA gene sequencing could be successfully used to study the bacterial diversity in mine tailings. The colonization of the mine tailings by reed significantly changed the bacterial community and the chemical properties of tailings. The complex interactions between bacteria and plants deserve further investigation. 展开更多
关键词 16s rRNA gene bacterial diversity bacterial community functional group mine tailings
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Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere 被引量:7
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作者 Likun Gu Zhihui Bai +4 位作者 Bo Jin Qing Hu Huili Wang Guoqiang Zhuang HongxunZhang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2010年第1期134-141,共8页
Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere.... Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the γ-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application. 展开更多
关键词 FUNGICIDE PHYLLOsPHERE microbial community 16s rDNA clone library PCR-DGGE
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Effects of reclaimed water irrigation and nitrogen fertilization on the chemical properties and microbial community of soil 被引量:11
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作者 GUO Wei Mathias N Andersen +4 位作者 QI Xue-bin LI Ping LI Zhong-yang FAN Xiang-yang ZHOU Yuan 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第3期679-690,共12页
The ecological effect of reclaimed water irrigation and fertilizer application on the soil environment is receiving more attention.Soil microbial activity and nitrogen(N)levels are important indicators of the effect... The ecological effect of reclaimed water irrigation and fertilizer application on the soil environment is receiving more attention.Soil microbial activity and nitrogen(N)levels are important indicators of the effect of reclaimed water irrigation on environment.This study evaluated soil physicochemical properties and microbial community structure in soils irrigated with reclaimed water and receiving varied amounts of N fertilizer.The results indicated that the reclaimed water irrigation increased soil electrical conductivity(EC)and soil water content(SWC).The N treatment has highly significant effect on the ACE,Chao,Shannon(H)and Coverage indices.Based on a 16S ribosomal RNA(16S rRNA)sequence analysis,the Proteobacteria,Gemmatimonadetes and Bacteroidetes were more abundant in soil irrigated with reclaimed water than in soil irrigated with clean water.Stronger clustering of microbial communities using either clean or reclaimed water for irrigation indicated that the type of irrigation water may have a greater influence on the structure of soil microbial community than N fertilizer treatment.Based on a canonical correspondence analysis(CCA)between the species of soil microbes and the chemical properties of the soil,which indicated that nitrate N(NO3-–-N)and total phosphorus(TP)had significant impact on abundance of Verrucomicrobia and Gemmatimonadetes,meanwhile the p H and organic matter(OM)had impact on abundance of Firmicutes and Actinobacteria significantly.It was beneficial to the improvement of soil bacterial activity and fertility under 120 mg kg^-1 N with reclaimed water irrigation. 展开更多
关键词 reclaimed water NITROGEN soil chemical properties 16s rRNA sequence soil microbe community
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Eubacteria and Archaea community of simultaneous methanogenesis and denitrification granular sludge 被引量:8
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作者 SUN Yujiao ZUO Jiane +1 位作者 CHEN Lili WANG Yong 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2008年第5期626-631,共6页
Based on the successful performance of a lab-scale upflow anaerobic sludge blanket (UASB) reactor with the capacity of simultaneous methanogenesis and denitrification (SMD), the specific phylogenetic groups and co... Based on the successful performance of a lab-scale upflow anaerobic sludge blanket (UASB) reactor with the capacity of simultaneous methanogenesis and denitrification (SMD), the specific phylogenetic groups and community structure of microbes in the SMD granule in the UASB reactor were investigated by the construction of the Eubacteria and Archaea 16S rDNA clone libraries, fragment length polymorphism, and sequence blast. Real time quantitative-polymerase chain reaction (RTQ-PCR) technique was used to quantify the contents of Eubacteria and Archaea in the SMD granule. The contents of some special predominant methanogens were also investigated. The results indicated that the Methanosaeta and Methanobacteria were the predominant methanogens in all Archaea in the SMD granule, with contents of 71.59% and 22.73% in all 88 random Archaea clones, respectively. The diversity of Eubacteria was much more complex than that of Archaea. The low GC positive gram bacteria and ε-Protebacteria were the main predominant Eubacteria species in SMD granule, their contents were 49.62% and 12.03% in all 133 random Eubacteria clones respectively. The results of RTQ-PCR indicated that the content of Archaea was less than Eubacteria, the Archaea content in total microorganisms in SMD granule was about 27.6%. 展开更多
关键词 simultaneous methanogenesis and denitrification sMD) granular sludge 16s rDNA clone libraries RTQ-PCR community structure
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