Forsythia fructus has been shown to have antioxidative, anti-inflammatory, antibacterial, anti-aging and whitening effects. Hoechunyangkyeok-san (Forsythia viridissima-prescription) is a traditional herbal medicine, w...Forsythia fructus has been shown to have antioxidative, anti-inflammatory, antibacterial, anti-aging and whitening effects. Hoechunyangkyeok-san (Forsythia viridissima-prescription) is a traditional herbal medicine, which has been clinically used for treating febrile and inflammatory disorders. This work was carried out to investigate the skin whitening effects of Forsythia viridissima-prescription extract (a hydrolyzed extract of Hoechunyangkyeok-san: SID White HYC) on skin. The effects of SID White HYC were assessed the melanin contents in B161 melanoma cells and the pigmented equivalent with HMB45 and Fontana Masson staining in 3D skin model. Then, we examined the expression of major pigment enzymes regulating melanin synthesis and melanosome transport related proteins in B16F1 cells. SID White HYC significantly inhibited the melanin synthesis (56.7% and 30.6% inhibition at 100 μg/mL, intracellular and secreted, respectively) in B16F1 cells and 3D skin model. In addition, western blotting analysis showed that SID White HYC reduced the expression of melanin synthesis and melanosome transport related proteins in B16F1 cells. In clinical trials, the cream containing 0.05% SID White HYC showed skin depigmentation effect without any irritation. These results suggest that SID White HYC may be useful inhibition of melanogenesis and melanosome transport. Therefore, SID White HYC may have potential as a skin-whitening ingredient in cosmetics.展开更多
阔叶杂草是苗圃中重要的危害性杂草,然而控制苗圃阔叶杂草的有效化学方法却很少,主要原因是能防除阔叶草的除草剂往往对苗木不安全或缺少对其安全性研究。选择甲嘧磺隆、乙氧氟草醚和扑草净3种杀草谱较广的除草剂,通过2015年田间小区试...阔叶杂草是苗圃中重要的危害性杂草,然而控制苗圃阔叶杂草的有效化学方法却很少,主要原因是能防除阔叶草的除草剂往往对苗木不安全或缺少对其安全性研究。选择甲嘧磺隆、乙氧氟草醚和扑草净3种杀草谱较广的除草剂,通过2015年田间小区试验,研究不同用量下这3种除草剂对苗圃常见苗木云南黄馨、金钟扦插苗成苗、发芽、生根、株高等因素的影响。结果显示:用药时雨水、土壤湿度可严重影响植物生长状况,雨水多、湿度大,则发根、出叶少;用药时间影响药剂效应,扦插前用药,药剂影响大于扦插后用药,这可能是扦插前、扦插后苗在遇药时的叶芽状态及水分不同所造成的。研究还发现,药剂对苗木成苗无不良影响,7.5 g a.i./hm^275%甲嘧磺隆WDG处理对苗木出叶、株高无影响;而在生根方面,在药后30 d,只有对照的71.1%,用药后100 d,与对照相当,乙氧氟草醚144、288 g a.i./hm^2,扑草净450、900 g a.i./hm^24个处理的结果与甲嘧磺隆7.5 g a.i./hm^2处理相同;对于甲嘧磺隆15 g a.i./hm^2处理,在前期对苗木出叶、生根都有较大影响,3个月后影响逐渐减少甚至消失;甲嘧磺隆22.5 g a.i./hm^2处理对苗木出叶、生根、株高的抑制可持续3个月以上。展开更多
文摘Forsythia fructus has been shown to have antioxidative, anti-inflammatory, antibacterial, anti-aging and whitening effects. Hoechunyangkyeok-san (Forsythia viridissima-prescription) is a traditional herbal medicine, which has been clinically used for treating febrile and inflammatory disorders. This work was carried out to investigate the skin whitening effects of Forsythia viridissima-prescription extract (a hydrolyzed extract of Hoechunyangkyeok-san: SID White HYC) on skin. The effects of SID White HYC were assessed the melanin contents in B161 melanoma cells and the pigmented equivalent with HMB45 and Fontana Masson staining in 3D skin model. Then, we examined the expression of major pigment enzymes regulating melanin synthesis and melanosome transport related proteins in B16F1 cells. SID White HYC significantly inhibited the melanin synthesis (56.7% and 30.6% inhibition at 100 μg/mL, intracellular and secreted, respectively) in B16F1 cells and 3D skin model. In addition, western blotting analysis showed that SID White HYC reduced the expression of melanin synthesis and melanosome transport related proteins in B16F1 cells. In clinical trials, the cream containing 0.05% SID White HYC showed skin depigmentation effect without any irritation. These results suggest that SID White HYC may be useful inhibition of melanogenesis and melanosome transport. Therefore, SID White HYC may have potential as a skin-whitening ingredient in cosmetics.
文摘阔叶杂草是苗圃中重要的危害性杂草,然而控制苗圃阔叶杂草的有效化学方法却很少,主要原因是能防除阔叶草的除草剂往往对苗木不安全或缺少对其安全性研究。选择甲嘧磺隆、乙氧氟草醚和扑草净3种杀草谱较广的除草剂,通过2015年田间小区试验,研究不同用量下这3种除草剂对苗圃常见苗木云南黄馨、金钟扦插苗成苗、发芽、生根、株高等因素的影响。结果显示:用药时雨水、土壤湿度可严重影响植物生长状况,雨水多、湿度大,则发根、出叶少;用药时间影响药剂效应,扦插前用药,药剂影响大于扦插后用药,这可能是扦插前、扦插后苗在遇药时的叶芽状态及水分不同所造成的。研究还发现,药剂对苗木成苗无不良影响,7.5 g a.i./hm^275%甲嘧磺隆WDG处理对苗木出叶、株高无影响;而在生根方面,在药后30 d,只有对照的71.1%,用药后100 d,与对照相当,乙氧氟草醚144、288 g a.i./hm^2,扑草净450、900 g a.i./hm^24个处理的结果与甲嘧磺隆7.5 g a.i./hm^2处理相同;对于甲嘧磺隆15 g a.i./hm^2处理,在前期对苗木出叶、生根都有较大影响,3个月后影响逐渐减少甚至消失;甲嘧磺隆22.5 g a.i./hm^2处理对苗木出叶、生根、株高的抑制可持续3个月以上。
