Detection of the vitality of wounds is essential in forensic practice.The present study used Illumina RNA-seq technology to determine gene expression profiles in contused mouse skin.In obtained high quality sequencing...Detection of the vitality of wounds is essential in forensic practice.The present study used Illumina RNA-seq technology to determine gene expression profiles in contused mouse skin.In obtained high quality sequencing reads,the reads were mapped onto a reference transcriptome (Mus_musculus.GRCm38.83).The results revealed that there were 659 upregulated and 996 down-regulated differentially expressed genes (DEGs) in contused mouse skin.The DEGs were further analyzed using the Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases.Genes from different functional categories and signalling pathways were enriched,including the immune system process,immune response,defense response,cytokine-cytokine receptor interaction,complement and coagulation cascades and chemokine signalling pathway.Expression patterns of 11 DEGs were verified by RT-qPCR in mice skins.In addition,alterations of five DEGs were also analyzed in postmortem human wound samples.The results were in concordance with the results of RNA-seq.These findings suggest that RNA-seq is a powerful tool to reveal DEGs as potential markers for vital reaction in terms of forensic practices.展开更多
In forensic practice,the identification of antemortem burns and postmortem burns is of the utmost importance.Reports from previous studies have shown that miRNAs,with lengths stretching over 18–25 nucleotides,are hig...In forensic practice,the identification of antemortem burns and postmortem burns is of the utmost importance.Reports from previous studies have shown that miRNAs,with lengths stretching over 18–25 nucleotides,are highly stable and resistant to degradation.However,there has been little research into the application of miRNAs in identifying antemortem and postmortem burns.This study compared the expression of miR-711 and miR-183-3p levels in mouse and postmortem human burned skins using RT-qPCR assay.RT-qPCR examination of burned mouse skins showed that increased miR-711 and miR-183-3p expression in comparison to intact skin tissues.The increased expressions of these two miRNAs were observed until 120 h after death in burned mouse skins,whereas no significant changes were found in postmortem burned skins.In human burned skins,the increased levels of these two miRNAs at 48 h following autopsy occurred in 19 of 26 subjects,which appeared to be related to the severity of the burn.These findings suggest that miR-711 and miR-183-3p may act as biomarkers for vital reaction of skin burn.展开更多
基金National Natural Science Foundation of China[grant num-bers 81430045 and 81401556]Natural Science Foundation of Guangdong Province[grant number 2014A030310293].
文摘Detection of the vitality of wounds is essential in forensic practice.The present study used Illumina RNA-seq technology to determine gene expression profiles in contused mouse skin.In obtained high quality sequencing reads,the reads were mapped onto a reference transcriptome (Mus_musculus.GRCm38.83).The results revealed that there were 659 upregulated and 996 down-regulated differentially expressed genes (DEGs) in contused mouse skin.The DEGs were further analyzed using the Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases.Genes from different functional categories and signalling pathways were enriched,including the immune system process,immune response,defense response,cytokine-cytokine receptor interaction,complement and coagulation cascades and chemokine signalling pathway.Expression patterns of 11 DEGs were verified by RT-qPCR in mice skins.In addition,alterations of five DEGs were also analyzed in postmortem human wound samples.The results were in concordance with the results of RNA-seq.These findings suggest that RNA-seq is a powerful tool to reveal DEGs as potential markers for vital reaction in terms of forensic practices.
基金supported by the National Natural Science Foundation of China[grant numbers 81871526 and 81601641]the Scientific Research Foundation for the Returned Overseas Chinese Scholarsand the National Education Ministry[grant number 2015-311].
文摘In forensic practice,the identification of antemortem burns and postmortem burns is of the utmost importance.Reports from previous studies have shown that miRNAs,with lengths stretching over 18–25 nucleotides,are highly stable and resistant to degradation.However,there has been little research into the application of miRNAs in identifying antemortem and postmortem burns.This study compared the expression of miR-711 and miR-183-3p levels in mouse and postmortem human burned skins using RT-qPCR assay.RT-qPCR examination of burned mouse skins showed that increased miR-711 and miR-183-3p expression in comparison to intact skin tissues.The increased expressions of these two miRNAs were observed until 120 h after death in burned mouse skins,whereas no significant changes were found in postmortem burned skins.In human burned skins,the increased levels of these two miRNAs at 48 h following autopsy occurred in 19 of 26 subjects,which appeared to be related to the severity of the burn.These findings suggest that miR-711 and miR-183-3p may act as biomarkers for vital reaction of skin burn.
