A high performance liquid chromatographic method for the simultaneous determination of four fat soluble vitamins: retinol (vitamin A), cholecalciferol(vitamin D), tocopherol(vitamin E) and phylloquinone(vitamin K_1) i...A high performance liquid chromatographic method for the simultaneous determination of four fat soluble vitamins: retinol (vitamin A), cholecalciferol(vitamin D), tocopherol(vitamin E) and phylloquinone(vitamin K_1) in fortified milk powders and egg yolk has been developed. The method requires enzymatic hydrolysis of lipid component of the sample as a pretreatment. Several factors which influence the enzymatic hydrolysis were studied-Separation was achieved using μ-Bondapak C-18 column(3.9×300mm), 98% methanol as mobile phase, a double channel detection was selected; vitamins D_3 E_1 K_1 were detected by UV spectrophotometry (265 nm) first, then vitamin A by fluorometry (EX 325nm, EM 480mm). The retention times of vitamin A_1 D_3, E and K_1 4.87, 9.00, 10.58 and 15.45 min respectively. Detection limit were 0.64, 0.25, 0.50 and 0.07 ng; and the recoveries were 90.5%～103.6% 90.0%～95.6%, 91.7%～98.8%, 91.5%～98.6%, respectively. The vitamins A, D_0, E, K_1 contents in foods were determined satisfactorily.展开更多
作者将1～2月龄的人工喂养儿随机分为两组,试验组食用维生素 D 强化牛奶,对照组食用普通牛奶另加鱼肝油,观察三个月。结果体格发育指标及血清钙、磷和碱性磷酸酶,两组间无显著性差异(P>0.05)。开始时未患佝偻病的21人,结束时仍保持...作者将1～2月龄的人工喂养儿随机分为两组,试验组食用维生素 D 强化牛奶,对照组食用普通牛奶另加鱼肝油,观察三个月。结果体格发育指标及血清钙、磷和碱性磷酸酶,两组间无显著性差异(P>0.05)。开始时未患佝偻病的21人,结束时仍保持正常。表吸两种补充方法均能收到预防佝偻病的效果,但每日另加鱼肝油既麻烦,且剂量也难以掌握。展开更多
文摘A high performance liquid chromatographic method for the simultaneous determination of four fat soluble vitamins: retinol (vitamin A), cholecalciferol(vitamin D), tocopherol(vitamin E) and phylloquinone(vitamin K_1) in fortified milk powders and egg yolk has been developed. The method requires enzymatic hydrolysis of lipid component of the sample as a pretreatment. Several factors which influence the enzymatic hydrolysis were studied-Separation was achieved using μ-Bondapak C-18 column(3.9×300mm), 98% methanol as mobile phase, a double channel detection was selected; vitamins D_3 E_1 K_1 were detected by UV spectrophotometry (265 nm) first, then vitamin A by fluorometry (EX 325nm, EM 480mm). The retention times of vitamin A_1 D_3, E and K_1 4.87, 9.00, 10.58 and 15.45 min respectively. Detection limit were 0.64, 0.25, 0.50 and 0.07 ng; and the recoveries were 90.5%～103.6% 90.0%～95.6%, 91.7%～98.8%, 91.5%～98.6%, respectively. The vitamins A, D_0, E, K_1 contents in foods were determined satisfactorily.
文摘作者将1～2月龄的人工喂养儿随机分为两组,试验组食用维生素 D 强化牛奶,对照组食用普通牛奶另加鱼肝油,观察三个月。结果体格发育指标及血清钙、磷和碱性磷酸酶,两组间无显著性差异(P>0.05)。开始时未患佝偻病的21人,结束时仍保持正常。表吸两种补充方法均能收到预防佝偻病的效果,但每日另加鱼肝油既麻烦,且剂量也难以掌握。
