Samples of fresh Volvariella volvaceae, dry seeds of Irvingia gabonensis and dry seeds of Beilschmiedia mannii), three traditional vegetables found on the market in Abidjan were studied in relation to their nutrients ...Samples of fresh Volvariella volvaceae, dry seeds of Irvingia gabonensis and dry seeds of Beilschmiedia mannii), three traditional vegetables found on the market in Abidjan were studied in relation to their nutrients composition (protein, fat, carbohydrates), energy value, moisture, ash and minerals (Ca, Na, K, Mg, Fe). The results show that: Volvariella vol- vaceae is mushroom distinguished by its high rate of moisture (81% ± 0.530% fresh matter), high carbohydrate content (79.440% ± 0.24% dry matter) and protein content (17.010% ± 0.04% dry matter) very significant. Mineral composition contains more Na (1.880% ± 0.02% dry matter) and K (1.260% ± 0.12% dry matter). Seed of Irvingia gabonensis is rich in lipid (63.610% ± 0.70% dry matter) but it also contains appreciable levels of carbohydrates (23.250% ± 0.17% dry matter), protein (7.230% ± 0. 80% dry matter) and mineral matter (5.910% ± 0, 30% dry matter). These seeds of Irvingia gabonensis contain more K (0.678% ± 0.01% dry matter) and Ca (0.452% ± 0.09% dry matter). Seed of Beil- schmiedia mannii is rich in carbohydrates (92.080% ± 1.20% dry matter), and has a significant protein content (7.160% ± 0.11% dry matter), its mineral composition contains more than K (0.872% ± 0.05% dry matter).展开更多
Cellulose binding domains (CBDs) are present in the majority of fungal cellulases and hemicellulases. Based on the conserved region of CBDs, degenerate primers were designed and used to amplify the 5′ end cDNA fragme...Cellulose binding domains (CBDs) are present in the majority of fungal cellulases and hemicellulases. Based on the conserved region of CBDs, degenerate primers were designed and used to amplify the 5′ end cDNA fragment of xylanase from Volvariella volvacea by 5′ RACE. Gene specific primer was then designed based on extreme region of 5′ end cDNA fragment and used to amplify the full length cDNA of xylanase. The cDNA of xyn 1 was 1 287 bp in length, including 3′ and 5′ non coding region. The xyn 1 cDNA contained an ORF of 1101 bp encoding 367 amino acids, in which there was a putative signal peptide with 19 amino acids. Alignment of the deduced amino acid sequence of xyn 1 with other xylanases showed that the homology with family 10 xylanases from Agaricus bisporus xyl1,Aspergillus sojae xyn1, Aspergillus kawachii xynA, Fusarium oxysporum f.sp xyl3 was 64%,55%,52%,55%, respectively.展开更多
Genetic diversity study of wild Philippine paddy straw mushroom （Volvariella volvacea） was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains...Genetic diversity study of wild Philippine paddy straw mushroom （Volvariella volvacea） was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains were collected from different geographical areas in Northern and Central Luzon region. Strains were differentiated using random amplified polymorphic DNA （RAPD）. A single 10-based primer was used to generate randomly amplified polymorphic DNA （RAPD） in V. volvacea and differences were noted in band size （bp） ranging from 1,800 bp to 550 bp. Principal component analysis （PCA） of the RAPD data revealed 4 groups from wild strains. One strain showed RAPD pattern with band appearance at 1,750, 950 and 750 bp; 3 strains at 1,800 and 750 bp; 8 strains at 1,500 and 550; and the most abundant group with 29 strains at 750 bp. With observed lack of heterogeneity among strains, it is recommended that more collections from the wild should be undertaken for more diverse germplasm collection. Moreover, it is suggested that RAPD can be used to delineate strains of V. volvacea with potential importance on genetic diversity conservation and breeding.展开更多
Volvariella volvacea is a popular variety because of its distinct flavor, pleasant taste, higher protein content and shorter cropping duration compared with other cultivated mushrooms. It is also the most expensive mu...Volvariella volvacea is a popular variety because of its distinct flavor, pleasant taste, higher protein content and shorter cropping duration compared with other cultivated mushrooms. It is also the most expensive mushroom in artificial mushrooms.With the development of economy and the industrialization of edible fungi, more and more straw mushroom has been cultivated in northern China, and southern mushroom is moving to northern areas. In this study, based on many years of cultivation experience, the high yield cultivating techniques of V. volvacea was summarized. V. volvacea cultivation consists of five steps, i.e.,composting, pasteurization, spawning, pining and harvesting.展开更多
Greenhouse summer squash is a major type of greenhouse vegetable in Shandong Province.In summer,there is a three-month idle period of greenhouse summer squash.Volvariella volvacea is a high temperature resistant high-...Greenhouse summer squash is a major type of greenhouse vegetable in Shandong Province.In summer,there is a three-month idle period of greenhouse summer squash.Volvariella volvacea is a high temperature resistant high-quality edible fungus.In practice,it is able to use the spent mushroom substrate of Flammulina velutipes to cultivate V.volvacea.In the idle period of greenhouse summer squash,cultivating V.volvacea using the spent mushroom substrate of F.velutipes has high economic and environmental benefits.The cultivation techniques mainly include the preparation of cultivation materials,preliminary preparation for the greenhouse,planting management of V.volvacea,and returning the spent mushroom substrate to the field.By comparison with the conventional summer squash planting,this technique has higher economic benefits and its application prospects will be broad.展开更多
文摘Samples of fresh Volvariella volvaceae, dry seeds of Irvingia gabonensis and dry seeds of Beilschmiedia mannii), three traditional vegetables found on the market in Abidjan were studied in relation to their nutrients composition (protein, fat, carbohydrates), energy value, moisture, ash and minerals (Ca, Na, K, Mg, Fe). The results show that: Volvariella vol- vaceae is mushroom distinguished by its high rate of moisture (81% ± 0.530% fresh matter), high carbohydrate content (79.440% ± 0.24% dry matter) and protein content (17.010% ± 0.04% dry matter) very significant. Mineral composition contains more Na (1.880% ± 0.02% dry matter) and K (1.260% ± 0.12% dry matter). Seed of Irvingia gabonensis is rich in lipid (63.610% ± 0.70% dry matter) but it also contains appreciable levels of carbohydrates (23.250% ± 0.17% dry matter), protein (7.230% ± 0. 80% dry matter) and mineral matter (5.910% ± 0, 30% dry matter). These seeds of Irvingia gabonensis contain more K (0.678% ± 0.01% dry matter) and Ca (0.452% ± 0.09% dry matter). Seed of Beil- schmiedia mannii is rich in carbohydrates (92.080% ± 1.20% dry matter), and has a significant protein content (7.160% ± 0.11% dry matter), its mineral composition contains more than K (0.872% ± 0.05% dry matter).
文摘Cellulose binding domains (CBDs) are present in the majority of fungal cellulases and hemicellulases. Based on the conserved region of CBDs, degenerate primers were designed and used to amplify the 5′ end cDNA fragment of xylanase from Volvariella volvacea by 5′ RACE. Gene specific primer was then designed based on extreme region of 5′ end cDNA fragment and used to amplify the full length cDNA of xylanase. The cDNA of xyn 1 was 1 287 bp in length, including 3′ and 5′ non coding region. The xyn 1 cDNA contained an ORF of 1101 bp encoding 367 amino acids, in which there was a putative signal peptide with 19 amino acids. Alignment of the deduced amino acid sequence of xyn 1 with other xylanases showed that the homology with family 10 xylanases from Agaricus bisporus xyl1,Aspergillus sojae xyn1, Aspergillus kawachii xynA, Fusarium oxysporum f.sp xyl3 was 64%,55%,52%,55%, respectively.
文摘Genetic diversity study of wild Philippine paddy straw mushroom （Volvariella volvacea） was conducted to establish a germplasm collection accessible to volvariella producers in the Philippines. Forty one wild strains were collected from different geographical areas in Northern and Central Luzon region. Strains were differentiated using random amplified polymorphic DNA （RAPD）. A single 10-based primer was used to generate randomly amplified polymorphic DNA （RAPD） in V. volvacea and differences were noted in band size （bp） ranging from 1,800 bp to 550 bp. Principal component analysis （PCA） of the RAPD data revealed 4 groups from wild strains. One strain showed RAPD pattern with band appearance at 1,750, 950 and 750 bp; 3 strains at 1,800 and 750 bp; 8 strains at 1,500 and 550; and the most abundant group with 29 strains at 750 bp. With observed lack of heterogeneity among strains, it is recommended that more collections from the wild should be undertaken for more diverse germplasm collection. Moreover, it is suggested that RAPD can be used to delineate strains of V. volvacea with potential importance on genetic diversity conservation and breeding.
基金Supported by the Earmarked Fund for Shanghai Modern Edible Fungi-industry Technology Research System(Grant No.201709)
文摘Volvariella volvacea is a popular variety because of its distinct flavor, pleasant taste, higher protein content and shorter cropping duration compared with other cultivated mushrooms. It is also the most expensive mushroom in artificial mushrooms.With the development of economy and the industrialization of edible fungi, more and more straw mushroom has been cultivated in northern China, and southern mushroom is moving to northern areas. In this study, based on many years of cultivation experience, the high yield cultivating techniques of V. volvacea was summarized. V. volvacea cultivation consists of five steps, i.e.,composting, pasteurization, spawning, pining and harvesting.
基金Sub-topics of National Key R&D program(2016YFD201306-03)Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2016B09).
文摘Greenhouse summer squash is a major type of greenhouse vegetable in Shandong Province.In summer,there is a three-month idle period of greenhouse summer squash.Volvariella volvacea is a high temperature resistant high-quality edible fungus.In practice,it is able to use the spent mushroom substrate of Flammulina velutipes to cultivate V.volvacea.In the idle period of greenhouse summer squash,cultivating V.volvacea using the spent mushroom substrate of F.velutipes has high economic and environmental benefits.The cultivation techniques mainly include the preparation of cultivation materials,preliminary preparation for the greenhouse,planting management of V.volvacea,and returning the spent mushroom substrate to the field.By comparison with the conventional summer squash planting,this technique has higher economic benefits and its application prospects will be broad.
