[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Resu...[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Result] The ob- tained sequence by usingthermal asymmetric interlaced PCRwas not ideal and the sequencing results were not matching to the known sequence. Though promoter se- quence of Hoxc8 in Mongolian Sheep was not obtained by thermal asymmetric in- terlaced PCR, but the results could provide references for the relevant studies in the future. [Conclusion] The research laid the foundation for further study on the methy- lation status Hoxc8 promoter in Mongolian Sheep.展开更多
In the present study we studied the genetic structure of five Chinese sheep populations of Hu sheep, Tong sheep, Small-tailed Han sheep, Tan sheep, and Wadi （WD） sheep using 15 microsatellite loci. The results showe...In the present study we studied the genetic structure of five Chinese sheep populations of Hu sheep, Tong sheep, Small-tailed Han sheep, Tan sheep, and Wadi （WD） sheep using 15 microsatellite loci. The results showed that the FIT, FST, and FIS statistics computed for the complete dataset had the following values, 0.523±0.140, 0.363±0.131 and 0.263±0.092, respectively. All loci were significantly contributed to the genetic differentiation among population （P0.001）. There is no relationship between the scatter of pairwise FST geographical distance points as geographical distance increases between the five populations. Membership probabilities and genetic structure of sheep populations were estimated when K=2, the populations were classified into Hu, Tong, Han and WD, and Tan sheep group. However, when K=3, the populations were classified into Hu and Tong, Han and WD, and Tan sheep group. The findings supported the previous literatures that these populations are originated on different time stages from the primogenitor population and communicated genetically with each other by natural and artificial selection in different ecological environment.展开更多
基金Supported by the Supporting Program of the"Eleventh Five-year Plan"for Sci&Tech Research of China(2006BDA13B08)National Natural Science Foundation of China(30960245)~~
文摘[Objective] The research aimed toexplore the method to obtain Hoxc8 pro- moter of Mongolian Sheep. [Method] Thermal asymmetric interlaced PCR was used to amplify the promoter sequence of Hoxc8 inMongolian Sheep. [Result] The ob- tained sequence by usingthermal asymmetric interlaced PCRwas not ideal and the sequencing results were not matching to the known sequence. Though promoter se- quence of Hoxc8 in Mongolian Sheep was not obtained by thermal asymmetric in- terlaced PCR, but the results could provide references for the relevant studies in the future. [Conclusion] The research laid the foundation for further study on the methy- lation status Hoxc8 promoter in Mongolian Sheep.
基金supported by the earmarked fund for China Agriculture Research System (CARS-39)the International Cooperation Item of the National Natural Science Foundation of China (30410103150)+7 种基金the China Postdoctoral Science Foundation Special Funded Project (200902154)the China Postdoctoral Science Foundation (20080430470)the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD13B08-07)the Natural Science Foundation of Jiangsu Province of China (BK2007556)the Basic Natural Science Foundation for Colleges and Universities, Jiangsu Province of China (NK051039)the Northern Jiangsu Technology Development Plan of China (BN2010004)the Science and Technology Program of Suzhou City of Jiangsu Province of China (SNG0911)the Jiangsu Government Scholarship for Overseas Studies Project, China
文摘In the present study we studied the genetic structure of five Chinese sheep populations of Hu sheep, Tong sheep, Small-tailed Han sheep, Tan sheep, and Wadi （WD） sheep using 15 microsatellite loci. The results showed that the FIT, FST, and FIS statistics computed for the complete dataset had the following values, 0.523±0.140, 0.363±0.131 and 0.263±0.092, respectively. All loci were significantly contributed to the genetic differentiation among population （P0.001）. There is no relationship between the scatter of pairwise FST geographical distance points as geographical distance increases between the five populations. Membership probabilities and genetic structure of sheep populations were estimated when K=2, the populations were classified into Hu, Tong, Han and WD, and Tan sheep group. However, when K=3, the populations were classified into Hu and Tong, Han and WD, and Tan sheep group. The findings supported the previous literatures that these populations are originated on different time stages from the primogenitor population and communicated genetically with each other by natural and artificial selection in different ecological environment.
