Objective To evaluate the safety and efficacy of early high intensity blood purification for severe wasp venom poisoning with acute kidney injury(AKI).Methods We retrospectively analyzed 120 patients[(47±14)year,...Objective To evaluate the safety and efficacy of early high intensity blood purification for severe wasp venom poisoning with acute kidney injury(AKI).Methods We retrospectively analyzed 120 patients[(47±14)year,F/M=68/52]with severe wasp venom poisoning(creatine kinase>20,000 IU/L;lactic展开更多
This study evaluated the effects of purified paper wasp Ropalidia marginata venoms on various biomolecules in the blood serum of albino mice. Changes in the concentration of some important macromolecules, i.e., protei...This study evaluated the effects of purified paper wasp Ropalidia marginata venoms on various biomolecules in the blood serum of albino mice. Changes in the concentration of some important macromolecules, i.e., proteins, free amino acids, uric acid, cholesterol, pyruvic acid, total lipids and glucose were noted down. These alterations were measured after intraperitoneal injection of 40% and 80% 24-hour LD50 purified Ropalidia marginata venom toxin. Serum total protein levels were found to decrease to 78% after 6 hrs, while serum free amino acid levels were significantly increased to 117% 6 hrs after venom injection compared to control. It was also found that serum uric acid levels increased to 138% after 8 hrs of venom injection compared to control. The increase in serum cholesterol i.e. (101% and 106%) and pyruvic acid increased significantly to a maximum value of 106% after 6 hrs of treatment at 40% LD<sub>50</sub>. Glycogen levels in the gastrocnemius muscle were found to decrease significantly (p-0.05) to 43% and 92% at LD<sub>50</sub> after injection of purified Ropalidia marginata venom after 8 h and 80% at LD<sub>50</sub> compared to control. Moreover, up to 71% and 81% were obtained at 10 hrs of treatment with the same dose. In the present study, the purified toxins significantly changed the levels of biomolecules in blood serum, indicating their wider effects on cellular physiology due to toxic effects and stress on the animal. These toxins can be good antigens and stimulate immune responses in experimental mice.展开更多
Yellow Wasp Polistes flavus venom toxins were isolated and purified on a Sepharose CL-6B 200 column. Purified proteins were investigated for its antibacterial and antifungal activity against 13 infectious microbial pa...Yellow Wasp Polistes flavus venom toxins were isolated and purified on a Sepharose CL-6B 200 column. Purified proteins were investigated for its antibacterial and antifungal activity against 13 infectious microbial pathogens. Paper disc diffusion and serial micro-dilution assays were performed for the determination of inhibition zone (DIZ) diameters and minimal inhibitory concentration, respectively. Triton X-100 (0.1%) proved to be a good solubilizing agent for toxin/proteins. Higher protein solubilization was observed in the supernatant than in the residue, except TCA (tri-chloroacetic acid). The elution pattern of purified and homogenized sting glands exhibited two major peaks at 280 nm in fraction No. 41 - 61 and 81 - 101. The total yield of protein was 69.21% and specific activity was determined in each fraction. Molecular weights in protein fractions were ranging from 6 - 70 kD. MIC (Minimum Inhibitory Concentration) values were 12.3 μg/ml against K. pneumonia 12.3 μg/ml against E. coli and L. acidophilus, 24.6 μg/ml against B. cereus;49.24 μg/ml against S. aureus and M. luteus. By agar disc diffusion method, the diameter of inhibition zones in mm in presence of yellow wasp toxins is at a concentration range of 98.56 - 6.9 μg/ml E. coli 18.36 ± 0.14, Bacillus cereus 14.566 ± 0.21, L. acidophilus 18.10 ± 0.21, Micrococcus luteus 18.76 ± 0.19, S. aeurus 17.36 ± 0.43, Klebsiella pneuminiae 19.56 ± 0.21, Salmonella typhi 19.96 ± 0.31, Vibrio cholera 21.83 ± 0.22, Pseudomonas aeruginosa 22.90 ± 0.09, Aspergillus niger 21.66 ± 0.16, Candida albicans 23.33 ± 0.26, Rhizopus stolonifer 24.96 ± 0.16 respectively. The antibacterial and antifungal activity of venom toxin may be due to action on cell membrane, its destruction and cell lysis. The wasp toxins may be used as strong biological agents to control microbes.展开更多
文摘Objective To evaluate the safety and efficacy of early high intensity blood purification for severe wasp venom poisoning with acute kidney injury(AKI).Methods We retrospectively analyzed 120 patients[(47±14)year,F/M=68/52]with severe wasp venom poisoning(creatine kinase>20,000 IU/L;lactic
文摘This study evaluated the effects of purified paper wasp Ropalidia marginata venoms on various biomolecules in the blood serum of albino mice. Changes in the concentration of some important macromolecules, i.e., proteins, free amino acids, uric acid, cholesterol, pyruvic acid, total lipids and glucose were noted down. These alterations were measured after intraperitoneal injection of 40% and 80% 24-hour LD50 purified Ropalidia marginata venom toxin. Serum total protein levels were found to decrease to 78% after 6 hrs, while serum free amino acid levels were significantly increased to 117% 6 hrs after venom injection compared to control. It was also found that serum uric acid levels increased to 138% after 8 hrs of venom injection compared to control. The increase in serum cholesterol i.e. (101% and 106%) and pyruvic acid increased significantly to a maximum value of 106% after 6 hrs of treatment at 40% LD<sub>50</sub>. Glycogen levels in the gastrocnemius muscle were found to decrease significantly (p-0.05) to 43% and 92% at LD<sub>50</sub> after injection of purified Ropalidia marginata venom after 8 h and 80% at LD<sub>50</sub> compared to control. Moreover, up to 71% and 81% were obtained at 10 hrs of treatment with the same dose. In the present study, the purified toxins significantly changed the levels of biomolecules in blood serum, indicating their wider effects on cellular physiology due to toxic effects and stress on the animal. These toxins can be good antigens and stimulate immune responses in experimental mice.
文摘Yellow Wasp Polistes flavus venom toxins were isolated and purified on a Sepharose CL-6B 200 column. Purified proteins were investigated for its antibacterial and antifungal activity against 13 infectious microbial pathogens. Paper disc diffusion and serial micro-dilution assays were performed for the determination of inhibition zone (DIZ) diameters and minimal inhibitory concentration, respectively. Triton X-100 (0.1%) proved to be a good solubilizing agent for toxin/proteins. Higher protein solubilization was observed in the supernatant than in the residue, except TCA (tri-chloroacetic acid). The elution pattern of purified and homogenized sting glands exhibited two major peaks at 280 nm in fraction No. 41 - 61 and 81 - 101. The total yield of protein was 69.21% and specific activity was determined in each fraction. Molecular weights in protein fractions were ranging from 6 - 70 kD. MIC (Minimum Inhibitory Concentration) values were 12.3 μg/ml against K. pneumonia 12.3 μg/ml against E. coli and L. acidophilus, 24.6 μg/ml against B. cereus;49.24 μg/ml against S. aureus and M. luteus. By agar disc diffusion method, the diameter of inhibition zones in mm in presence of yellow wasp toxins is at a concentration range of 98.56 - 6.9 μg/ml E. coli 18.36 ± 0.14, Bacillus cereus 14.566 ± 0.21, L. acidophilus 18.10 ± 0.21, Micrococcus luteus 18.76 ± 0.19, S. aeurus 17.36 ± 0.43, Klebsiella pneuminiae 19.56 ± 0.21, Salmonella typhi 19.96 ± 0.31, Vibrio cholera 21.83 ± 0.22, Pseudomonas aeruginosa 22.90 ± 0.09, Aspergillus niger 21.66 ± 0.16, Candida albicans 23.33 ± 0.26, Rhizopus stolonifer 24.96 ± 0.16 respectively. The antibacterial and antifungal activity of venom toxin may be due to action on cell membrane, its destruction and cell lysis. The wasp toxins may be used as strong biological agents to control microbes.
