Four peptides PSPP1,PSPP2,PSPP3 and PSPP4 were purified from the water-extract of Papaver somniferum pollen.Their sequences,with 21,17,13 and 16 amino acid residues respectively,have been determined by Edman degradati...Four peptides PSPP1,PSPP2,PSPP3 and PSPP4 were purified from the water-extract of Papaver somniferum pollen.Their sequences,with 21,17,13 and 16 amino acid residues respectively,have been determined by Edman degradation-N-terminal dansylation.PSPP2, PSPP3 and PSPP4 were synthesized using solid phase method.The immunopromotive activities of PSPP1,PSPP2,PSPP3,PSPP4 and the initially separated sample PSPP have been also observed by the methods of counting erythrocyte rosette forming cells(ERFC) and T-lymphocyte transformation test in vitro.展开更多
[Objectives]This study was conducted to optimize the soaking conditions of Miao medicine Xiange Zuyu Powder.[Methods]With the water-soluble extract as an investigation index,an L_(9)(3^(4))orthogonal test was carried ...[Objectives]This study was conducted to optimize the soaking conditions of Miao medicine Xiange Zuyu Powder.[Methods]With the water-soluble extract as an investigation index,an L_(9)(3^(4))orthogonal test was carried out to investigate the amount of water added,soaking temperature,soaking times and soaking time,and the best soaking conditions for Xiange Zuyu Powder were screened.[Results]The optimal soaking conditions were as follows:adding 1000 ml of boiling water at 100℃,soaking for 20 min each time,3 times in total.[Conclusions]The soaking scheme is reasonable and can provide a reasonable basis for clinical medication.展开更多
文摘Four peptides PSPP1,PSPP2,PSPP3 and PSPP4 were purified from the water-extract of Papaver somniferum pollen.Their sequences,with 21,17,13 and 16 amino acid residues respectively,have been determined by Edman degradation-N-terminal dansylation.PSPP2, PSPP3 and PSPP4 were synthesized using solid phase method.The immunopromotive activities of PSPP1,PSPP2,PSPP3,PSPP4 and the initially separated sample PSPP have been also observed by the methods of counting erythrocyte rosette forming cells(ERFC) and T-lymphocyte transformation test in vitro.
基金Supported by Intra-college Scientific Research Project of Guizhou University of Traditional Chinese Medicine(3040-040210084,3040-04020001406)。
文摘[Objectives]This study was conducted to optimize the soaking conditions of Miao medicine Xiange Zuyu Powder.[Methods]With the water-soluble extract as an investigation index,an L_(9)(3^(4))orthogonal test was carried out to investigate the amount of water added,soaking temperature,soaking times and soaking time,and the best soaking conditions for Xiange Zuyu Powder were screened.[Results]The optimal soaking conditions were as follows:adding 1000 ml of boiling water at 100℃,soaking for 20 min each time,3 times in total.[Conclusions]The soaking scheme is reasonable and can provide a reasonable basis for clinical medication.
