In order to explore the effects of amino acid foliar fertilizer on the yield and quality of different vegetables, we tested the spraying of an amino acid foliar fertilizer(Wanfeng 2) onto the leaves of cucumbers, eggp...In order to explore the effects of amino acid foliar fertilizer on the yield and quality of different vegetables, we tested the spraying of an amino acid foliar fertilizer(Wanfeng 2) onto the leaves of cucumbers, eggplants and water spinach through contrast experiment in a greenhouse. The results showed that the yield increments of amino acid foliar fertilizer treatments in cucumbers, eggplants and water spinach were 11.57%, 4.43% and 12.41% respectively, and the output-input ratios were 64.52 ∶ 1, 53.31 ∶ 1 and 55.14 ∶ 1 respectively. Amino acid foliar fertilizer can influence the quality of greenhouse vegetables. The total sugar content of the cucumbers, eggplants, and water spinach significantly increased by 12.00%, 22.30%and 12.10% respectively. The vitamin C content increased by 4.76%, 24.80%, and10.90% respectively compared with the control. In conclusion, the spraying of amino acid foliar fertilizer could improve the yield and the nutritive quality of vegetables such as cucumbers, eggplants and water spinach.展开更多
[Objective] The aim was to explore the effect of amino acid fertilizer on the growth, development and quality of flue-cured tobacco. [Method] With Yunyan 87 as tested flue-cured tobacco varieties, three treatments (c...[Objective] The aim was to explore the effect of amino acid fertilizer on the growth, development and quality of flue-cured tobacco. [Method] With Yunyan 87 as tested flue-cured tobacco varieties, three treatments (chemical fertilizer (control), chemical fertilizer+solid amino acid, chemical fertilizer+liquid amino acid) were set up to study the effects of amino acid fertilizer on flue-cured tobacco fertilizer reducing and efficiency increasing by means of contrast in the same field. [Result] The flue-cured tobacco applied solid amino acid fertilizer had the best performances of overall agronomic traits and the highest yield, while liquid amino acid fertilizer was conductive to the top leaf opening, making the raw tobacco appearance best, and could increase the proportion of middle and high quality tobacco, gaining good economic benefits. The flue-cured tobacco treated by liquid amino acid fertilizer had the optimal ratio of total sugar to nicotine and disaccharide differences in tobacco leaves, and the best coordination of chemical composition. Compared with the contrel, tobacco treated by amino acid fertilizer had less irritating and more comfortable taste, and the tobacco treated by liquid amino acid had light miscellaneous gas, and the best overall performance of sensory quality. [Conclusion] Chemical fertilizer+liquid amino acids is conductive to promote the growth and development of flue-cured tobacco and improve the quality of tobacco leaves.展开更多
[Objectives]This study was conducted to explore the treatment methods of amino acid selenium fertilizer suitable for increasing the selenium content of mangoes and improving the quality of mangoes,so as to provide a t...[Objectives]This study was conducted to explore the treatment methods of amino acid selenium fertilizer suitable for increasing the selenium content of mangoes and improving the quality of mangoes,so as to provide a theoretical basis for the production of selenium-enriched mangoes.[Methods]With Tainong mango as a test material,the amino acid selenium foliar fertilizer was applied by eight treatment methods to investigate the changes of selenium,soluble solid,vitamin C,and titratable acid contents in mango flesh and peel.[Results]Spraying amino acid selenium foliar fertilizer increased the selenium content of mango flesh.The selenium contents of the treatment groups T2,T3,T4 and T5 reached the selenium-enriched standard,and the T2 treatment group had the highest selenium content(0.020 mg/kg).The selenium contents in the peel of all treatment groups were greater than the corresponding selenium content in the flesh.Except for T1,the vitamin C contents of other treatment groups sprayed with amino acid selenium fertilizer increased compared with the control,and that of the T2 treatment group increased significantly(P<0.05).Compared with the control,the soluble solids of the treatment groups sprayed with amino acid selenium fertilizer once also increased.The treatment method of spraying amino acid selenium fertilizer with a dosage of 12000 ml/hm2 once(T2)achieved the best effect of increasing the selenium content and improving the quality of mangoes.[Conclusions]This study provides a scientific basis for increasing the selenium content of mangoes and improving the current situation of insufficient selenium intake.展开更多
The experiment aimed to investigate foliar fertilizer application effects on amino acid content of Indigofera leaves. lndigofera arrecta was planted in plots during both rainy and dry seasons. Foliar fertilizer concen...The experiment aimed to investigate foliar fertilizer application effects on amino acid content of Indigofera leaves. lndigofera arrecta was planted in plots during both rainy and dry seasons. Foliar fertilizer concentrations consisted of 10 g, 20 g, 30 g, 40 g and 50 g, which solved in 10 L solvent were appIied on sixteen individual plants per plot, The fertilizer was applied 4 times during vegetative growing period. Block randomized design with 3 replications was used in this experiment. The foliar fertilizer containing 20%-22% N (N total), 15%-16% P (P205), 15%-16% K, 1.5% Mg, 1% Ca, 1% Fe, 1.42% Zn, 0.53% Cu, 0.88% Mo, 0.53% B and growth hormone 0.025% indole acetic acid (1AA) and giberelic acid (GA) was applied. Samples of each plot were composited leaves from 3 harvest times. The result showed that the foliar fertilizer did not affect aspartic acid, seriue, histidine, threonine, tyrosine, valine, methionine, iso-leusine and leusine content oflndigofera leave. Foliar fertilizer application up to 50 g/10 L resulted in higher (P 〈 0.05) glutamate acid, glysine, arginine, alanine, proline and lysine than those of other fertilizer levels.展开更多
In order to select suitable mini watermelon varieties for spring greenhouse cultivation under substrate cultivation,the field character,yield character,quality character and trade trait of six new mini watermelon vari...In order to select suitable mini watermelon varieties for spring greenhouse cultivation under substrate cultivation,the field character,yield character,quality character and trade trait of six new mini watermelon varieties with red flesh were compared in the test.The results showed that mini watermelon applied with alanine and arginine had obvious advantages in quality character,yield character and trade trait.The yields reached 3876.8 and 44120.20 kg/667m2;the weight of single melon reached 1.98 and 2.10 kg;and the central sugar content reached 13.34%and 13.25%,respectively.Meantime,mini watermelon was juicy,sweet,tasty and refreshing,and was suitable for planting under substrate cultivation.展开更多
We analyzed the amino acid residues present in the water-soluble and transmembrane proteins of 6 thermophilic and 6 mesophilic species of the domains Archaea and Eubacteria, and characterized them as favorable or unfa...We analyzed the amino acid residues present in the water-soluble and transmembrane proteins of 6 thermophilic and 6 mesophilic species of the domains Archaea and Eubacteria, and characterized them as favorable or unfavorable. The characterization was performed by comparing the observed number of each amino acid residue to the expected number calculated from the percentage of nucleotides present in each gene. Amino acids that were more or less abundant than expected were considered as favorable or unfavorable, respectively. Comparisons of amino acid compositions indicated that the water-soluble proteins were rich in charged residues such as Glu, Asp, Lys, and His, whereas hydrophobic residues such as Trp, Phe, and Leu were abundant in transmembrane proteins. Interestingly, our results found that although the Trp residue was abundant in transmembrane proteins, it was not defined as favorable by our calculations, indicating that increased numbers of a particular amino acid does not necessary indicate it is a favorable residue. Amino acids with high G + C content such as Ala, Gly, and Pro were frequently observed as favorable in species with low G + C content. Comparatively, amino acids with low G + C content such as Phe, Tyr, Lys, Ile, and Met were frequently observed as favorable in species with high G + C content. These are the examples to increase the supply of amino acids than expected. Amino acids with neutral G + C content, i.e., Glu and Asp were favorable in water-soluble proteins from all species analyzed, and Cys was unfavorable both in water-soluble and transmembrane proteins. These results indicate that amino acid compositions are essentially determined by the nucleotide sequence of the genes, and the amino acid content is altered by a deviation from expectation.展开更多
To explore fertilization methods for wine bamboo cultivation in southwestern semi-arid areas of China, this study analyzed annual changes in sap yield and nutrient composition from May 2013 to March 2015 by using bamb...To explore fertilization methods for wine bamboo cultivation in southwestern semi-arid areas of China, this study analyzed annual changes in sap yield and nutrient composition from May 2013 to March 2015 by using bamboo charcoal-based bio-fertilizer (ZT) and organic fertilizer treatments (CK). The study also provided basic data for functional beverage preparation and for application of ZT. The results of the two experimental cycles revealed that under the ZT treatment, sap was available for collection from May, the beginning of the rainy season, to November, the beginning of the dry season. The period of abundance was July to October with the highest yield of sap of 3.18 L stalk-1 in September, 2014, still lower than the moso bamboo sap, which was likely due to the scale of sap production of monopodial bamboos being different from that of sympodial bamboos. In January, trace amounts of sap were still detected, suggesting that the effect of the treatment was significant. Moreover,in the dry season, soil water content and soil temperatures at 10-15 cm depths indicated that the fertilizer had the ability to maintain soil temperatures and moisture. In both fertilizer treatments, the correlation between the collected sap and environmental parameters was significant. In the ZT treatment for the entire 2 years, the effectual environ- mental factors were soil water at 10-15 cm, air tempera- tures, and wind speeds. The same determining factors were observed for the rainy season. In the CK treatments, the effectual environmental factors for the entire year and the rainy season were soil water at 0-5 cm and air moisture. The bamboo charcoal-based bio-fertilizer elevated the potassium, calcium, iron, manganese, copper, and total phosphorus content, simultaneously increasing the sap yield, protein and reducing sugar contents, and with a relative increase in sap pH. The wine bamboo sap con- tained 18 amino acids. Glutamic acid, alanine and proline were the most abundant. Compared to the controls, the treatment showed higher levels of all amino acids. Thus, the ZT treatment could be more beneficial to the development of root systems because the function of heat preservation and moisture retention prolong the sap collection period, increase sap yields, and elevate mineral element, conventional nutrients, and amino acid contents with evident fertilization effects and broader application prospects.展开更多
The amino acids and the volatile substances in grapes and wines play important roles in their quality,and the concentrations of these substances can be changed by how a vineyard is managed,e.g.,irrigation and fertigat...The amino acids and the volatile substances in grapes and wines play important roles in their quality,and the concentrations of these substances can be changed by how a vineyard is managed,e.g.,irrigation and fertigation regimes.This study aimed to evaluate the effect of fertilizer and water management on the distribution of amino acids,the volatile component profiles,and the sensory characteristics of Cabernet Sauvignon grapes and wines.The results showed that the amino acid concentration in grape berries was the highest under the 100%local fertilizer rate(HF)and 100%water irrigation quota(HW)treatment,and the volatile component concentration in wine was the highest under HF and 80%water irrigation quota(MW)treatment.The effect of irrigation on the amino acid content in grapes was greater than that of fertigation.The synergistic effect of fertilizer and water on arginine,serine,and glutamine in grape berries was significant.The interactive effect of fertigation and irrigation on the volatile substance in grapes was greater than that of fertigation and irrigation alone.The influence of irrigation on volatile substances in wines was greater than that of fertigation.In addition,there was also a correlation between the concentrations of multiple amino acids in grapes and volatile components in wines.Principal component analysis showed that the wine from the HFMW treatment had the best quality among all treatments.展开更多
Nutrient loading in the fall is a practical way to improve seedling quality and has been proven to increase nutrient accumulation,translocation and utilization.Few studies have reported on the variation in free amino ...Nutrient loading in the fall is a practical way to improve seedling quality and has been proven to increase nutrient accumulation,translocation and utilization.Few studies have reported on the variation in free amino acids as a result of fall fertilization,especially for diff erent seasonal needle habits(evergreen,deciduous).Therefore,a balanced two-factor factorial design with one fall fertilization treatment(10 mg N/seedling)and Chinese pine(Pinus tabulaeformis Carr.)and Prince Rupprecht’s larch(Larix principis-rupprechtii Mayr.)seedlings was used to examine growth response over one nursery season.Associated changes between fall fertilization,N storage and free amino acids were analyzed.Results showed that:(1)stem height,diameter and biomass for both species were similar between controls and fall fertilization treatments;(2)compared to controls,fall fertilization increased Chinese pine needle and root N by 17.7%and 36.9%,respectively.For Prince Rupprecht’s larch,fall fertilization resulted in 26.3%and 34.54%more N in stem and roots,respectively,than controls;(3)the three main amino acids in control and fertilization treatments in Prince Rupprecht’s larch seedlings were glutamine,arginine and proline,and in Chinese pine seedlings were glutamine,arginine andγ-amino butyric acid;(4)total amino acid contents were not signifi cantly increased by fall fertilization,but glutamine in Chinese pine and Prince Rupprecht’s larch increased by 64.2%and 35.2%,respectively.Aboveground biomass of Prince Rupprecht’s larch had higher proline contents than Chinese pine,which suggests that the stress resistance of the aboveground tissue may be higher for Prince Rupprecht’s larch.The results indicate that diff erent plant organs with various response are well adapted to nitrogen loading for nutrient storage in evergreen and deciduous conifer seedlings.展开更多
基金Supported by National Science and Technology Support Program(2015BAD 23B00)National Key Research&Development Project(2016YFD0300901-03)~~
文摘In order to explore the effects of amino acid foliar fertilizer on the yield and quality of different vegetables, we tested the spraying of an amino acid foliar fertilizer(Wanfeng 2) onto the leaves of cucumbers, eggplants and water spinach through contrast experiment in a greenhouse. The results showed that the yield increments of amino acid foliar fertilizer treatments in cucumbers, eggplants and water spinach were 11.57%, 4.43% and 12.41% respectively, and the output-input ratios were 64.52 ∶ 1, 53.31 ∶ 1 and 55.14 ∶ 1 respectively. Amino acid foliar fertilizer can influence the quality of greenhouse vegetables. The total sugar content of the cucumbers, eggplants, and water spinach significantly increased by 12.00%, 22.30%and 12.10% respectively. The vitamin C content increased by 4.76%, 24.80%, and10.90% respectively compared with the control. In conclusion, the spraying of amino acid foliar fertilizer could improve the yield and the nutritive quality of vegetables such as cucumbers, eggplants and water spinach.
基金Supported by the Research and Application of PVY Disease Control Technology in Shaotong Tobacco Planting Area of the Science and Technology Program of Yunnan Company of China Tobacco Cooperation(2015YN2)~~
文摘[Objective] The aim was to explore the effect of amino acid fertilizer on the growth, development and quality of flue-cured tobacco. [Method] With Yunyan 87 as tested flue-cured tobacco varieties, three treatments (chemical fertilizer (control), chemical fertilizer+solid amino acid, chemical fertilizer+liquid amino acid) were set up to study the effects of amino acid fertilizer on flue-cured tobacco fertilizer reducing and efficiency increasing by means of contrast in the same field. [Result] The flue-cured tobacco applied solid amino acid fertilizer had the best performances of overall agronomic traits and the highest yield, while liquid amino acid fertilizer was conductive to the top leaf opening, making the raw tobacco appearance best, and could increase the proportion of middle and high quality tobacco, gaining good economic benefits. The flue-cured tobacco treated by liquid amino acid fertilizer had the optimal ratio of total sugar to nicotine and disaccharide differences in tobacco leaves, and the best coordination of chemical composition. Compared with the contrel, tobacco treated by amino acid fertilizer had less irritating and more comfortable taste, and the tobacco treated by liquid amino acid had light miscellaneous gas, and the best overall performance of sensory quality. [Conclusion] Chemical fertilizer+liquid amino acids is conductive to promote the growth and development of flue-cured tobacco and improve the quality of tobacco leaves.
基金Guangxi Science and Technology Major Project(GK AA17202037-2,GK AA17202026)Guangxi Mango Innovation Team Cultivation Post Project of National Modern Agriculture Industrial Technology System(nycytxgxcxtd-06-02)+4 种基金Special Fund of Central Government for Local Science and Technology Development(GK ZY19183007)Special Action of the Science and Technology Vanguard of Guangxi Academy of Agricultural Sciences(GNK JZ202016)Guangxi Key R&D Program(GK AB16380164)Guangxi Selenium-enriched Crop Experiment Station(GTS2016011)Fundamental Scientific Research Fund of Guangxi Academy of Agricultural Sciences(GNK 2020YM109)。
文摘[Objectives]This study was conducted to explore the treatment methods of amino acid selenium fertilizer suitable for increasing the selenium content of mangoes and improving the quality of mangoes,so as to provide a theoretical basis for the production of selenium-enriched mangoes.[Methods]With Tainong mango as a test material,the amino acid selenium foliar fertilizer was applied by eight treatment methods to investigate the changes of selenium,soluble solid,vitamin C,and titratable acid contents in mango flesh and peel.[Results]Spraying amino acid selenium foliar fertilizer increased the selenium content of mango flesh.The selenium contents of the treatment groups T2,T3,T4 and T5 reached the selenium-enriched standard,and the T2 treatment group had the highest selenium content(0.020 mg/kg).The selenium contents in the peel of all treatment groups were greater than the corresponding selenium content in the flesh.Except for T1,the vitamin C contents of other treatment groups sprayed with amino acid selenium fertilizer increased compared with the control,and that of the T2 treatment group increased significantly(P<0.05).Compared with the control,the soluble solids of the treatment groups sprayed with amino acid selenium fertilizer once also increased.The treatment method of spraying amino acid selenium fertilizer with a dosage of 12000 ml/hm2 once(T2)achieved the best effect of increasing the selenium content and improving the quality of mangoes.[Conclusions]This study provides a scientific basis for increasing the selenium content of mangoes and improving the current situation of insufficient selenium intake.
文摘The experiment aimed to investigate foliar fertilizer application effects on amino acid content of Indigofera leaves. lndigofera arrecta was planted in plots during both rainy and dry seasons. Foliar fertilizer concentrations consisted of 10 g, 20 g, 30 g, 40 g and 50 g, which solved in 10 L solvent were appIied on sixteen individual plants per plot, The fertilizer was applied 4 times during vegetative growing period. Block randomized design with 3 replications was used in this experiment. The foliar fertilizer containing 20%-22% N (N total), 15%-16% P (P205), 15%-16% K, 1.5% Mg, 1% Ca, 1% Fe, 1.42% Zn, 0.53% Cu, 0.88% Mo, 0.53% B and growth hormone 0.025% indole acetic acid (1AA) and giberelic acid (GA) was applied. Samples of each plot were composited leaves from 3 harvest times. The result showed that the foliar fertilizer did not affect aspartic acid, seriue, histidine, threonine, tyrosine, valine, methionine, iso-leusine and leusine content oflndigofera leave. Foliar fertilizer application up to 50 g/10 L resulted in higher (P 〈 0.05) glutamate acid, glysine, arginine, alanine, proline and lysine than those of other fertilizer levels.
文摘In order to select suitable mini watermelon varieties for spring greenhouse cultivation under substrate cultivation,the field character,yield character,quality character and trade trait of six new mini watermelon varieties with red flesh were compared in the test.The results showed that mini watermelon applied with alanine and arginine had obvious advantages in quality character,yield character and trade trait.The yields reached 3876.8 and 44120.20 kg/667m2;the weight of single melon reached 1.98 and 2.10 kg;and the central sugar content reached 13.34%and 13.25%,respectively.Meantime,mini watermelon was juicy,sweet,tasty and refreshing,and was suitable for planting under substrate cultivation.
文摘We analyzed the amino acid residues present in the water-soluble and transmembrane proteins of 6 thermophilic and 6 mesophilic species of the domains Archaea and Eubacteria, and characterized them as favorable or unfavorable. The characterization was performed by comparing the observed number of each amino acid residue to the expected number calculated from the percentage of nucleotides present in each gene. Amino acids that were more or less abundant than expected were considered as favorable or unfavorable, respectively. Comparisons of amino acid compositions indicated that the water-soluble proteins were rich in charged residues such as Glu, Asp, Lys, and His, whereas hydrophobic residues such as Trp, Phe, and Leu were abundant in transmembrane proteins. Interestingly, our results found that although the Trp residue was abundant in transmembrane proteins, it was not defined as favorable by our calculations, indicating that increased numbers of a particular amino acid does not necessary indicate it is a favorable residue. Amino acids with high G + C content such as Ala, Gly, and Pro were frequently observed as favorable in species with low G + C content. Comparatively, amino acids with low G + C content such as Phe, Tyr, Lys, Ile, and Met were frequently observed as favorable in species with high G + C content. These are the examples to increase the supply of amino acids than expected. Amino acids with neutral G + C content, i.e., Glu and Asp were favorable in water-soluble proteins from all species analyzed, and Cys was unfavorable both in water-soluble and transmembrane proteins. These results indicate that amino acid compositions are essentially determined by the nucleotide sequence of the genes, and the amino acid content is altered by a deviation from expectation.
基金supported by Zhejiang Provincial Natural Science Foundation of China(LY14C030008)Forestry Industry Standard Project of China(2015LY-080)
文摘To explore fertilization methods for wine bamboo cultivation in southwestern semi-arid areas of China, this study analyzed annual changes in sap yield and nutrient composition from May 2013 to March 2015 by using bamboo charcoal-based bio-fertilizer (ZT) and organic fertilizer treatments (CK). The study also provided basic data for functional beverage preparation and for application of ZT. The results of the two experimental cycles revealed that under the ZT treatment, sap was available for collection from May, the beginning of the rainy season, to November, the beginning of the dry season. The period of abundance was July to October with the highest yield of sap of 3.18 L stalk-1 in September, 2014, still lower than the moso bamboo sap, which was likely due to the scale of sap production of monopodial bamboos being different from that of sympodial bamboos. In January, trace amounts of sap were still detected, suggesting that the effect of the treatment was significant. Moreover,in the dry season, soil water content and soil temperatures at 10-15 cm depths indicated that the fertilizer had the ability to maintain soil temperatures and moisture. In both fertilizer treatments, the correlation between the collected sap and environmental parameters was significant. In the ZT treatment for the entire 2 years, the effectual environ- mental factors were soil water at 10-15 cm, air tempera- tures, and wind speeds. The same determining factors were observed for the rainy season. In the CK treatments, the effectual environmental factors for the entire year and the rainy season were soil water at 0-5 cm and air moisture. The bamboo charcoal-based bio-fertilizer elevated the potassium, calcium, iron, manganese, copper, and total phosphorus content, simultaneously increasing the sap yield, protein and reducing sugar contents, and with a relative increase in sap pH. The wine bamboo sap con- tained 18 amino acids. Glutamic acid, alanine and proline were the most abundant. Compared to the controls, the treatment showed higher levels of all amino acids. Thus, the ZT treatment could be more beneficial to the development of root systems because the function of heat preservation and moisture retention prolong the sap collection period, increase sap yields, and elevate mineral element, conventional nutrients, and amino acid contents with evident fertilization effects and broader application prospects.
基金the S&T Program of Hebei,China(Grant No.20327001D)the leading Talent Project of Science and Technology Innovation in Ningxia Hui Autonomous Region(Grant No.2022GKLRLX07).
文摘The amino acids and the volatile substances in grapes and wines play important roles in their quality,and the concentrations of these substances can be changed by how a vineyard is managed,e.g.,irrigation and fertigation regimes.This study aimed to evaluate the effect of fertilizer and water management on the distribution of amino acids,the volatile component profiles,and the sensory characteristics of Cabernet Sauvignon grapes and wines.The results showed that the amino acid concentration in grape berries was the highest under the 100%local fertilizer rate(HF)and 100%water irrigation quota(HW)treatment,and the volatile component concentration in wine was the highest under HF and 80%water irrigation quota(MW)treatment.The effect of irrigation on the amino acid content in grapes was greater than that of fertigation.The synergistic effect of fertilizer and water on arginine,serine,and glutamine in grape berries was significant.The interactive effect of fertigation and irrigation on the volatile substance in grapes was greater than that of fertigation and irrigation alone.The influence of irrigation on volatile substances in wines was greater than that of fertigation.In addition,there was also a correlation between the concentrations of multiple amino acids in grapes and volatile components in wines.Principal component analysis showed that the wine from the HFMW treatment had the best quality among all treatments.
基金We gratefully acknowledge the reviewers and many other members from the Beijing Forestry University for their insightful comments of the manuscript.
文摘Nutrient loading in the fall is a practical way to improve seedling quality and has been proven to increase nutrient accumulation,translocation and utilization.Few studies have reported on the variation in free amino acids as a result of fall fertilization,especially for diff erent seasonal needle habits(evergreen,deciduous).Therefore,a balanced two-factor factorial design with one fall fertilization treatment(10 mg N/seedling)and Chinese pine(Pinus tabulaeformis Carr.)and Prince Rupprecht’s larch(Larix principis-rupprechtii Mayr.)seedlings was used to examine growth response over one nursery season.Associated changes between fall fertilization,N storage and free amino acids were analyzed.Results showed that:(1)stem height,diameter and biomass for both species were similar between controls and fall fertilization treatments;(2)compared to controls,fall fertilization increased Chinese pine needle and root N by 17.7%and 36.9%,respectively.For Prince Rupprecht’s larch,fall fertilization resulted in 26.3%and 34.54%more N in stem and roots,respectively,than controls;(3)the three main amino acids in control and fertilization treatments in Prince Rupprecht’s larch seedlings were glutamine,arginine and proline,and in Chinese pine seedlings were glutamine,arginine andγ-amino butyric acid;(4)total amino acid contents were not signifi cantly increased by fall fertilization,but glutamine in Chinese pine and Prince Rupprecht’s larch increased by 64.2%and 35.2%,respectively.Aboveground biomass of Prince Rupprecht’s larch had higher proline contents than Chinese pine,which suggests that the stress resistance of the aboveground tissue may be higher for Prince Rupprecht’s larch.The results indicate that diff erent plant organs with various response are well adapted to nitrogen loading for nutrient storage in evergreen and deciduous conifer seedlings.