Water-Soluble Chitosan(WSC)has been sucessfuly synthesized from squid pens waste.The synthesis of chitosan from chitin was carried out by optimization of deacetylation temperature and time.Chitin was obtained from squ...Water-Soluble Chitosan(WSC)has been sucessfuly synthesized from squid pens waste.The synthesis of chitosan from chitin was carried out by optimization of deacetylation temperature and time.Chitin was obtained from squid pens waste by demineralization and deproteinization process.HCl 7%was used for demineralization and NaOH 10%at 60℃ was applied for deproteinization process.Deacetylation reaction was carried out at varied temperatures i.e.,60℃,70℃,80℃,90℃ and 100℃ in NaOH 50%solution for 10 hours.Deacetylation reaction time were varied for 2 hours,4 hours,6 hours,8 hours,and 10 hours.The crude chitosan obtained then reacted with H2O230%to depolymerize.The synthesis product obtained then characterized by FTIR.The result of squid chitin yield was 33.9%.The optimum temperature and time of chitosan deacetylation process were 90℃ for 8 hours as indicated by the value of deacetylation degree(DD)that equal to 83.94%at optimum temperature and 82.22%at optimum reaction time.The percentage of WSC yield at optimum temperature(90℃)and optimum time(8 hours)were 27.59%and 23.16%,respectively.WSC solubility test was done in water and HCl 0,1N.The solubility of 2.8325 mg/mL and 0.8125 mg/mL were obtained in acid medium and water medium,respectively.展开更多
Water-soluble chitosan(WSC)has been studied for its ability to mobilize soil Pb and promote the phytoremediation by Hylotelephium spectabile in Pb-contaminated fields.We aimed to clarify the internal mechanism by whic...Water-soluble chitosan(WSC)has been studied for its ability to mobilize soil Pb and promote the phytoremediation by Hylotelephium spectabile in Pb-contaminated fields.We aimed to clarify the internal mechanism by which wSC impacts phytoremediation by examining plant growth and Pb accumulation performance of H.spectabile as well as the Pb form,functional groups,and mineral phases of Pb-contaminated soil.WSC effectively decreased soil pH and activated Pb migration in rhizosphere soils,with a considerable increase in water-soluble and acid-extractable Pb by 29%-102%and 9%-65%,respectively,and a clear decreasing trend in reducible and oxidizable Pb.Fourier-transform infrared spectroscopy revealed a significant increase in amino and hydroxyl groups in the soil generated by WSC.The coordination of Pb with amino and hydroxyl groups may play an important role in the formation of Pb complexes and activation of Pb in soil.In field trials,the application of WSC significantly increased Pb accumulation in H.spectabile by 125.44%,reaching 92 g/hm^(2).Moreover,the organic matter and nitrogen in the soils were increased by WSc,which improved the growth conditions of H.spectabile.No obvious growth inhibition was observed in either the pot or field trials.Therefore,WSC is a promising chelating agent for mobilizing Pb in soil.Additionally,WSC can be potentially used to boost H.spectabil-mediated phytoremediation of Pb-contaminatedfarmland.展开更多
为研究WSC-DTPA(水溶性低分子量壳聚糖WSC,二乙烯三胺五乙酸DTPA)纳米粒的辐射防护作用,采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒;MTT法检测其对6 Gy60Coγ射线照射后48 h BRL细胞存活率的影响;活细胞工作站...为研究WSC-DTPA(水溶性低分子量壳聚糖WSC,二乙烯三胺五乙酸DTPA)纳米粒的辐射防护作用,采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒;MTT法检测其对6 Gy60Coγ射线照射后48 h BRL细胞存活率的影响;活细胞工作站观察BRL细胞摄取FITC-WSC-DTPA纳米荧光探针的情况。结果表明:成功合成了游离氨基含量分别为92.7%、74.3%、1.59%的WSC-DTPA聚合物;WSC、WSC纳米粒以及WSC-DTPA纳米粒(氨基含量为92.7%,浓度在6.25μg/mL以上),随着药物浓度的增加,BRL细胞存活率均显著高于单纯照射组,差别有统计学意义(p<0.05),而游离氨基含量为1.59%的WSC-DTPA纳米粒无辐射保护作用;活细胞工作站检验结果显示2 h内WSC纳米粒、WSC-DTPA纳米粒能够进入BRL细胞,而非纳米化的WSC-DTPA聚合物无法进入细胞。展开更多
Amphiphilic CS-SA polymers were prepared using the SA modified by small molecule water-soluble chitosan, and CS-SA nanomicelles and FF/CS-SA nanomicelles were prepared by using CSSA polymers as the material with dialy...Amphiphilic CS-SA polymers were prepared using the SA modified by small molecule water-soluble chitosan, and CS-SA nanomicelles and FF/CS-SA nanomicelles were prepared by using CSSA polymers as the material with dialysis-ultrasound method. CS-SA polymers, CS-SA nanomicelles, and FF/CS-SA nanomicelles were characterized by FT-IR, TGA, and SEM. Results showed that the SA was grafted to the amino of CS by amide bond. CS-SA nanomicelles and FF/CS-SA nanomicelles were spherical, smooth without fold. The influence of different molar ratio of FF to CS-SA polymers on the encapsulation efficiency and drug-loading rate determined the best molar ratio that was 1：1.09. In vitro release experiments, drug release performance study found that hydrophobic drug releasing from FF/CS-SA nanomicelles presented sustainedrelease. In vitro bacteriostasis experiments, when the concentration was higher than 4.98 mg/mL, FF/CSSA nanomicelles had antibacterial action and a positive correlation with concentration. The results revealed that amphiphilic chitosan derivative nanomicelles were carriers with broad prospects, increasing solubility of hydrophobic drugs and presenting sustained release for hydrophobic drugs, which provided a new research idea for drug delivery and targeted drug delivery of hydrohobic drugs.展开更多
High MW chitosan (CS) solutions have already been proposed as vehicles for protein delivery. The aim of the present work is to investigate the potential utility of water-soluble chitosan (WSC) as vehicles to load and ...High MW chitosan (CS) solutions have already been proposed as vehicles for protein delivery. The aim of the present work is to investigate the potential utility of water-soluble chitosan (WSC) as vehicles to load and deliver proteins. WSC nanoparticles (WSC NP) with various formations were prepared based on ionic gelation of WSC with pentasodium tripolyphosphate (TPP) anions. Bovine serum albumin (BSA) was used as a model protein drug incorporated into the WSC nanoparticles. Blank and BSA-loaded WSC nanoparticles were examined and determined to have a spherical shape with diameters between 35―190 nm, and zeta potential between 35―42 mV. FTIR confirmed that the tripolyphosphoric groups of TPP linked to the ammonium groups of WSC in the nanoparticles. Some factors affecting delivery properties of BSA have been investigated. Altering the concentration of BSA from 0.05 to 1 mg/mL enhanced the loading capacity of BSA but decreased loading efficiency simultaneously. Also, with the introduction of poly ethylene glycol (PEG), BSA release accelerated. Nanoparticle preparation from WSC with various deacetylation degrees (DDs) from 72.6% to 90% and MWs ranging from 3.5 to 15.8 kDa promoted loading efficiency and decreased the release rate. These results indicate that WSC nanoparticles are promising carriers for protein delivery.展开更多
Introduction: Benign Prostatic Hypertrophy (BPH) and Lower Urinary Tract Symptoms (LUTS) frequently affect the male population after the age of 50. Moreover, it is well known that there is a correlation of these sympt...Introduction: Benign Prostatic Hypertrophy (BPH) and Lower Urinary Tract Symptoms (LUTS) frequently affect the male population after the age of 50. Moreover, it is well known that there is a correlation of these symptoms with Erectile Dysfunction (ED) that can reach a frequency of roughly 52% in men aged between 40 and 70 years old. In this study, we aimed at evaluating the effect of a new natural compound “Tradamixina TP” (composed of 80 mg of the algae Ecklonia Bicyclis, 100 mg of Tribulus terrestris and 100 mg of Chitosan Water-soluble oligosaccharide 320 mg of Serenoa repens) in the improvement of Lower Urinary Tract Symptoms (LUTS). Materials & Methods: One-hundred patients with an average age of 64 years old and who were not taking any medication or phytotherapy for prostatic hypertrophy or erectile dysfunction were recruited in the study. All participants were subjected to serum prostate antigen (PSA) and uroflowmetry measuring maximal urine flow/sec (Qmax), at baseline and after 12 weeks. In addition, patients were asked to take the Tradamixina TP for 3 months and to complete at the beginning and end of the treatment the following questionnaires: 1) the IPSS investigating the level of LUTS, and 2) the IIEF-15 investigating the erectile dysfunction. Results: After the treatment, the IPSS scores were lower than those before the treatment. Moreover, a significant effect was found for the IIEF erectile subdomain with scores that were significantly higher at 12 weeks than at baseline. A similar result was found for the IIEF overall subdomain. A decrease of total serum PSA was also observed. Conclusions: After 3 months of daily treatment, the new compound “Tradamixina TP” improved patients’ sexual function, LUTS and led also to a decrease in serum PSA levels.展开更多
基金funded by Institute for Research and Community Services(LPPM,Lembaga Penelitian and Pengabdian Masyarakat)ITS(1438/PKS/ITS/2018)The research facilities were supported by Chemistry Department,Faculty Sciences,Institut Teknologi Sepuluh Nopember,Surabaya(ITS).
文摘Water-Soluble Chitosan(WSC)has been sucessfuly synthesized from squid pens waste.The synthesis of chitosan from chitin was carried out by optimization of deacetylation temperature and time.Chitin was obtained from squid pens waste by demineralization and deproteinization process.HCl 7%was used for demineralization and NaOH 10%at 60℃ was applied for deproteinization process.Deacetylation reaction was carried out at varied temperatures i.e.,60℃,70℃,80℃,90℃ and 100℃ in NaOH 50%solution for 10 hours.Deacetylation reaction time were varied for 2 hours,4 hours,6 hours,8 hours,and 10 hours.The crude chitosan obtained then reacted with H2O230%to depolymerize.The synthesis product obtained then characterized by FTIR.The result of squid chitin yield was 33.9%.The optimum temperature and time of chitosan deacetylation process were 90℃ for 8 hours as indicated by the value of deacetylation degree(DD)that equal to 83.94%at optimum temperature and 82.22%at optimum reaction time.The percentage of WSC yield at optimum temperature(90℃)and optimum time(8 hours)were 27.59%and 23.16%,respectively.WSC solubility test was done in water and HCl 0,1N.The solubility of 2.8325 mg/mL and 0.8125 mg/mL were obtained in acid medium and water medium,respectively.
基金The research was financially supported by the National Natural Science Foundation of China(Nos.42277237,22206149,and 41907125)the Applied Basic Research Project of Shanxi Province(China)(No.20210302124220)the Horizontal Scientific Research Funds of Taiyuan University of Technology(Nos.RH2200002532 and RH2300002821).
文摘Water-soluble chitosan(WSC)has been studied for its ability to mobilize soil Pb and promote the phytoremediation by Hylotelephium spectabile in Pb-contaminated fields.We aimed to clarify the internal mechanism by which wSC impacts phytoremediation by examining plant growth and Pb accumulation performance of H.spectabile as well as the Pb form,functional groups,and mineral phases of Pb-contaminated soil.WSC effectively decreased soil pH and activated Pb migration in rhizosphere soils,with a considerable increase in water-soluble and acid-extractable Pb by 29%-102%and 9%-65%,respectively,and a clear decreasing trend in reducible and oxidizable Pb.Fourier-transform infrared spectroscopy revealed a significant increase in amino and hydroxyl groups in the soil generated by WSC.The coordination of Pb with amino and hydroxyl groups may play an important role in the formation of Pb complexes and activation of Pb in soil.In field trials,the application of WSC significantly increased Pb accumulation in H.spectabile by 125.44%,reaching 92 g/hm^(2).Moreover,the organic matter and nitrogen in the soils were increased by WSc,which improved the growth conditions of H.spectabile.No obvious growth inhibition was observed in either the pot or field trials.Therefore,WSC is a promising chelating agent for mobilizing Pb in soil.Additionally,WSC can be potentially used to boost H.spectabil-mediated phytoremediation of Pb-contaminatedfarmland.
文摘为研究WSC-DTPA(水溶性低分子量壳聚糖WSC,二乙烯三胺五乙酸DTPA)纳米粒的辐射防护作用,采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒;MTT法检测其对6 Gy60Coγ射线照射后48 h BRL细胞存活率的影响;活细胞工作站观察BRL细胞摄取FITC-WSC-DTPA纳米荧光探针的情况。结果表明:成功合成了游离氨基含量分别为92.7%、74.3%、1.59%的WSC-DTPA聚合物;WSC、WSC纳米粒以及WSC-DTPA纳米粒(氨基含量为92.7%,浓度在6.25μg/mL以上),随着药物浓度的增加,BRL细胞存活率均显著高于单纯照射组,差别有统计学意义(p<0.05),而游离氨基含量为1.59%的WSC-DTPA纳米粒无辐射保护作用;活细胞工作站检验结果显示2 h内WSC纳米粒、WSC-DTPA纳米粒能够进入BRL细胞,而非纳米化的WSC-DTPA聚合物无法进入细胞。
基金the Science and Technology Development Project of Shandong Province(No.2012GNC11307)the People's Livelihood Science and Technology Project of Qingdao(NO.14232nsh)
文摘Amphiphilic CS-SA polymers were prepared using the SA modified by small molecule water-soluble chitosan, and CS-SA nanomicelles and FF/CS-SA nanomicelles were prepared by using CSSA polymers as the material with dialysis-ultrasound method. CS-SA polymers, CS-SA nanomicelles, and FF/CS-SA nanomicelles were characterized by FT-IR, TGA, and SEM. Results showed that the SA was grafted to the amino of CS by amide bond. CS-SA nanomicelles and FF/CS-SA nanomicelles were spherical, smooth without fold. The influence of different molar ratio of FF to CS-SA polymers on the encapsulation efficiency and drug-loading rate determined the best molar ratio that was 1：1.09. In vitro release experiments, drug release performance study found that hydrophobic drug releasing from FF/CS-SA nanomicelles presented sustainedrelease. In vitro bacteriostasis experiments, when the concentration was higher than 4.98 mg/mL, FF/CSSA nanomicelles had antibacterial action and a positive correlation with concentration. The results revealed that amphiphilic chitosan derivative nanomicelles were carriers with broad prospects, increasing solubility of hydrophobic drugs and presenting sustained release for hydrophobic drugs, which provided a new research idea for drug delivery and targeted drug delivery of hydrohobic drugs.
文摘High MW chitosan (CS) solutions have already been proposed as vehicles for protein delivery. The aim of the present work is to investigate the potential utility of water-soluble chitosan (WSC) as vehicles to load and deliver proteins. WSC nanoparticles (WSC NP) with various formations were prepared based on ionic gelation of WSC with pentasodium tripolyphosphate (TPP) anions. Bovine serum albumin (BSA) was used as a model protein drug incorporated into the WSC nanoparticles. Blank and BSA-loaded WSC nanoparticles were examined and determined to have a spherical shape with diameters between 35―190 nm, and zeta potential between 35―42 mV. FTIR confirmed that the tripolyphosphoric groups of TPP linked to the ammonium groups of WSC in the nanoparticles. Some factors affecting delivery properties of BSA have been investigated. Altering the concentration of BSA from 0.05 to 1 mg/mL enhanced the loading capacity of BSA but decreased loading efficiency simultaneously. Also, with the introduction of poly ethylene glycol (PEG), BSA release accelerated. Nanoparticle preparation from WSC with various deacetylation degrees (DDs) from 72.6% to 90% and MWs ranging from 3.5 to 15.8 kDa promoted loading efficiency and decreased the release rate. These results indicate that WSC nanoparticles are promising carriers for protein delivery.
文摘Introduction: Benign Prostatic Hypertrophy (BPH) and Lower Urinary Tract Symptoms (LUTS) frequently affect the male population after the age of 50. Moreover, it is well known that there is a correlation of these symptoms with Erectile Dysfunction (ED) that can reach a frequency of roughly 52% in men aged between 40 and 70 years old. In this study, we aimed at evaluating the effect of a new natural compound “Tradamixina TP” (composed of 80 mg of the algae Ecklonia Bicyclis, 100 mg of Tribulus terrestris and 100 mg of Chitosan Water-soluble oligosaccharide 320 mg of Serenoa repens) in the improvement of Lower Urinary Tract Symptoms (LUTS). Materials & Methods: One-hundred patients with an average age of 64 years old and who were not taking any medication or phytotherapy for prostatic hypertrophy or erectile dysfunction were recruited in the study. All participants were subjected to serum prostate antigen (PSA) and uroflowmetry measuring maximal urine flow/sec (Qmax), at baseline and after 12 weeks. In addition, patients were asked to take the Tradamixina TP for 3 months and to complete at the beginning and end of the treatment the following questionnaires: 1) the IPSS investigating the level of LUTS, and 2) the IIEF-15 investigating the erectile dysfunction. Results: After the treatment, the IPSS scores were lower than those before the treatment. Moreover, a significant effect was found for the IIEF erectile subdomain with scores that were significantly higher at 12 weeks than at baseline. A similar result was found for the IIEF overall subdomain. A decrease of total serum PSA was also observed. Conclusions: After 3 months of daily treatment, the new compound “Tradamixina TP” improved patients’ sexual function, LUTS and led also to a decrease in serum PSA levels.
