An acute toxicity test in Sprague Dawley(SD)rats and a chromosomal aberration toxicity test in Chinese hamster lung(CHL)fibroblasts were conducted to promote the application of insect wax and its policosanol.Results o...An acute toxicity test in Sprague Dawley(SD)rats and a chromosomal aberration toxicity test in Chinese hamster lung(CHL)fibroblasts were conducted to promote the application of insect wax and its policosanol.Results of oral acute toxicity test showed that the LD;values of insect wax and its policosanol were higher than 5000 mg/kg.The rats showed no obvious toxic symptoms and survived,suggesting that these substances were not toxic.The chromosomal aberration rates of the CHL cells incubated with insect wax for 3-6 h under metabolic activation conditions and for 3-6 h and 24 h under non-metabolic activation conditions were lower than 5%.No significant difference was found compared with the negative control group.Also,no significant difference in chromosomal aberration rates was found between each dose group of policosanol under metabolic or non-metabolic activation conditions and the negative control group.Therefore,the chromosomal aberration rates of CHL cells treated with insect wax and its policosanol were negative regardless of metabolic activation conditions.These results indicate the absence of acute toxicity and potential chromosomal aberration in the tested dose range of insect wax and its policosanol.展开更多
The ultra_morphology and chemical composition of waxes secreted by the scale insect Physokermes shanxiensis (Homoptera: Coccidae) was researched with the techniques of scanning electron microscope (SEM), infrared abso...The ultra_morphology and chemical composition of waxes secreted by the scale insect Physokermes shanxiensis (Homoptera: Coccidae) was researched with the techniques of scanning electron microscope (SEM), infrared absorption spectra (IR) and gas chromatography/mass spectrometry (GC/MS). The result indicated that the scale insect secreted wet wax in its nymph stage. The wet wax coagulated into translucent wax covering on the body surface. However, the female adult secreted its wax secretion with white color and long thread form. According to the infrared absorption spectra characteristic of the waxes, it can be known that the both waxes either from male nymph or female adult were composed by a series of saturated and unsaturated long carbon chain compounds. But the two waxes have different in the chemical functional groups kind and numbers that composed the compounds, because of the differences in absorption peaks numbers and forms which appeared in the two infrared spectra from the wave number 1 800 cm -1 to 1 000 cm -1 . Through detected with GC/MS, 14 compounds with carbon atom number form C 13 to C 32 were obtained form the wax of the male scale insect. These compounds included 5 saturated higher fatty acids, 1 unsaturated higher fatty acid, 1 saturated higher fatty alcohol, 1 unsaturated higher fatty alcohol, 1 unsaturated ester, 1 saturated aldehyde, and 4 saturated alkane compounds.展开更多
基金the support of the Fundamental Research Funds for the Central Non-profit Research Institution of CAF(CAFYBB2018SY025)the National High Technology Research and Development Program of China(863 Program)(2014AA021801)。
文摘An acute toxicity test in Sprague Dawley(SD)rats and a chromosomal aberration toxicity test in Chinese hamster lung(CHL)fibroblasts were conducted to promote the application of insect wax and its policosanol.Results of oral acute toxicity test showed that the LD;values of insect wax and its policosanol were higher than 5000 mg/kg.The rats showed no obvious toxic symptoms and survived,suggesting that these substances were not toxic.The chromosomal aberration rates of the CHL cells incubated with insect wax for 3-6 h under metabolic activation conditions and for 3-6 h and 24 h under non-metabolic activation conditions were lower than 5%.No significant difference was found compared with the negative control group.Also,no significant difference in chromosomal aberration rates was found between each dose group of policosanol under metabolic or non-metabolic activation conditions and the negative control group.Therefore,the chromosomal aberration rates of CHL cells treated with insect wax and its policosanol were negative regardless of metabolic activation conditions.These results indicate the absence of acute toxicity and potential chromosomal aberration in the tested dose range of insect wax and its policosanol.
文摘The ultra_morphology and chemical composition of waxes secreted by the scale insect Physokermes shanxiensis (Homoptera: Coccidae) was researched with the techniques of scanning electron microscope (SEM), infrared absorption spectra (IR) and gas chromatography/mass spectrometry (GC/MS). The result indicated that the scale insect secreted wet wax in its nymph stage. The wet wax coagulated into translucent wax covering on the body surface. However, the female adult secreted its wax secretion with white color and long thread form. According to the infrared absorption spectra characteristic of the waxes, it can be known that the both waxes either from male nymph or female adult were composed by a series of saturated and unsaturated long carbon chain compounds. But the two waxes have different in the chemical functional groups kind and numbers that composed the compounds, because of the differences in absorption peaks numbers and forms which appeared in the two infrared spectra from the wave number 1 800 cm -1 to 1 000 cm -1 . Through detected with GC/MS, 14 compounds with carbon atom number form C 13 to C 32 were obtained form the wax of the male scale insect. These compounds included 5 saturated higher fatty acids, 1 unsaturated higher fatty acid, 1 saturated higher fatty alcohol, 1 unsaturated higher fatty alcohol, 1 unsaturated ester, 1 saturated aldehyde, and 4 saturated alkane compounds.
