Macroporous poly [(chloromethyl) styrene-co-divinylbenzene] continuous rod was prepared by direct polymerization of the monomers in the presence of a porogenic diluent inside an empty chromatographic column. A new ...Macroporous poly [(chloromethyl) styrene-co-divinylbenzene] continuous rod was prepared by direct polymerization of the monomers in the presence of a porogenic diluent inside an empty chromatographic column. A new 'in-situ' technique was used to modify the synthesized polymer rod for a weak cation exchanger and it has been used successfully for the separation of biopolymers. It was found that the back pressure of the continuous rod column was much lower and its surface was proved to be modified well.展开更多
Monodisperse poly (chloromethylstyrene divinylbenzene) particles were prepared by a two-step swelling method. It was demonstrated that the packings have comparative advantages for biopolymer separation with high colum...Monodisperse poly (chloromethylstyrene divinylbenzene) particles were prepared by a two-step swelling method. It was demonstrated that the packings have comparative advantages for biopolymer separation with high column efficiency, low interstitial volume and low back pressure to the packings prepared with suspension polymerization method. Using size exclusionchromatography and weak ion exchange chromatography as examples, it was shown that these packings could be modified chemically more easily than poly (styrene divinylbenzene) packings.展开更多
A novel stationary phase for weak cation exchange （WCX） chromatography was prepared by ＂grafting from＂ strategy. Surface initiated atom transfer radical polymerization （ATRP） of acrylic acid （AA） was conducted...A novel stationary phase for weak cation exchange （WCX） chromatography was prepared by ＂grafting from＂ strategy. Surface initiated atom transfer radical polymerization （ATRP） of acrylic acid （AA） was conducted in toluene medium, starting from the macromolecule initiators of poly（4-vinylbenzyl chloride-co-divinylbenzene） （PcMs/DvB） beads, The amounts of poly（acrylic acid） grafted chains with different ATRP formulations were calculated based on the elemental analyses. The poly（acrylic acid） grafted beads obtained with different ATRP formula- tions were tried as chromatographic packings in the separation of proteins by ion-exchange chromatography. The effect of the poly（acrylic acid） grafted chain lengths on PCMS/DVB beads for the separation of proteins was investigated in details. Simultaneously, characterization of the column was investigated as ion chromatographic stationary phase for the separation of inorganic cations. The results show that poly（acrylic acid） grafted columns had excellent performance for separation of proteins and inorganic cations. The highest of the dynamic capacity of the column was 35.55 mg/mL. The columns were provided with high column efficiency.展开更多
The monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads with macroporous in the range of 8.0—12.0 m were prepared by a single-step swelling and polymerization method. The seed particles prepared...The monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads with macroporous in the range of 8.0—12.0 m were prepared by a single-step swelling and polymerization method. The seed particles prepared by dispersion polymerization exhibited good absorption of the monomer phase. The pore size distribution of the beads was evaluated by gel permeation chromatography and mercury intrusion method. By using this media, a weak cation exchange (WCX) stationary phase for HPLC was synthesized by a new chemical modification method. The prepared resin has advantages of biopolymer separation, high column efficiency, low column backpressure, high protein mass recovery and good resolution for proteins. The measured bioactivity recovery for lysozyme was (96±5)%. The dynamic protein loading capacity of the synthesized WCX packings was 21.3 mg/g. Five proteins were completely separated in 8.0 min using the synthesized WCX stationary phase. The experimental results show that the obtained WCX resin has very weak hydrophobicity. The WCX resin was also used for the rapid separation and purification of lysozyme from egg white in 8 min with only one step . The purity and specific bioactivity of the purified lysozyme was found more than 92.0% and 70184 U/mg, respectively.展开更多
文摘Macroporous poly [(chloromethyl) styrene-co-divinylbenzene] continuous rod was prepared by direct polymerization of the monomers in the presence of a porogenic diluent inside an empty chromatographic column. A new 'in-situ' technique was used to modify the synthesized polymer rod for a weak cation exchanger and it has been used successfully for the separation of biopolymers. It was found that the back pressure of the continuous rod column was much lower and its surface was proved to be modified well.
文摘Monodisperse poly (chloromethylstyrene divinylbenzene) particles were prepared by a two-step swelling method. It was demonstrated that the packings have comparative advantages for biopolymer separation with high column efficiency, low interstitial volume and low back pressure to the packings prepared with suspension polymerization method. Using size exclusionchromatography and weak ion exchange chromatography as examples, it was shown that these packings could be modified chemically more easily than poly (styrene divinylbenzene) packings.
基金Project supported by the Foundation of the Ministery of Science and Technology of China （No. 2009CB626608） and the National Natural Science Foundation of China （No. 201065008）.
文摘A novel stationary phase for weak cation exchange （WCX） chromatography was prepared by ＂grafting from＂ strategy. Surface initiated atom transfer radical polymerization （ATRP） of acrylic acid （AA） was conducted in toluene medium, starting from the macromolecule initiators of poly（4-vinylbenzyl chloride-co-divinylbenzene） （PcMs/DvB） beads, The amounts of poly（acrylic acid） grafted chains with different ATRP formulations were calculated based on the elemental analyses. The poly（acrylic acid） grafted beads obtained with different ATRP formula- tions were tried as chromatographic packings in the separation of proteins by ion-exchange chromatography. The effect of the poly（acrylic acid） grafted chain lengths on PCMS/DVB beads for the separation of proteins was investigated in details. Simultaneously, characterization of the column was investigated as ion chromatographic stationary phase for the separation of inorganic cations. The results show that poly（acrylic acid） grafted columns had excellent performance for separation of proteins and inorganic cations. The highest of the dynamic capacity of the column was 35.55 mg/mL. The columns were provided with high column efficiency.
基金Project supported by the National Natural Science Foundation of China (Nos. 39880003 and 20175016 ).
文摘The monodisperse poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads with macroporous in the range of 8.0—12.0 m were prepared by a single-step swelling and polymerization method. The seed particles prepared by dispersion polymerization exhibited good absorption of the monomer phase. The pore size distribution of the beads was evaluated by gel permeation chromatography and mercury intrusion method. By using this media, a weak cation exchange (WCX) stationary phase for HPLC was synthesized by a new chemical modification method. The prepared resin has advantages of biopolymer separation, high column efficiency, low column backpressure, high protein mass recovery and good resolution for proteins. The measured bioactivity recovery for lysozyme was (96±5)%. The dynamic protein loading capacity of the synthesized WCX packings was 21.3 mg/g. Five proteins were completely separated in 8.0 min using the synthesized WCX stationary phase. The experimental results show that the obtained WCX resin has very weak hydrophobicity. The WCX resin was also used for the rapid separation and purification of lysozyme from egg white in 8 min with only one step . The purity and specific bioactivity of the purified lysozyme was found more than 92.0% and 70184 U/mg, respectively.
