AIM: To examine the effect of Weichang'an(WCA) and 5-fluorouracil(5-Fu) on colorectal tumor and hepatic metastasis in a mouse model. METHODS: Quantitative determination of hesperidin, the effective component in WC...AIM: To examine the effect of Weichang'an(WCA) and 5-fluorouracil(5-Fu) on colorectal tumor and hepatic metastasis in a mouse model. METHODS: Quantitative determination of hesperidin, the effective component in WCA decoction, was performed using Hp LC. In vitro cytotoxicity of WCA was determined by treating HCT-116 cells with WCA diluents or serum extracted from rats that received WCA by oral gavage for 1 wk and MTT assays. Forty-eight nude mice received cecum implantation with tumor blocks subcutaneously amplified from human colon cancer cell line HCT-116. Mice were randomly divided into four treatment groups: control(CON), WCA, 5-Fu and combination(WCA + 5-Fu). pathological examination of tumors consisted of tissue sectioning and hematoxylin and eosin staining. Tumor weight and size were measured, and the number of metastatic l e s i o n s wa s c o u n t e d. S e r u m c a r c i n o e m b r yo n i c antigen(CEA) level was determined by ELISA. The expression levels of tumor genesis and metastasisrelated proteins β-catenin and matrix metalloproteinase(MMp)-7 were measured by real-time quantitative reverse transcriptase polymerase chain reaction(RTp CR), immunohistochemistry and immunoblotting. Cell fractionation was used to investigate intracellular distribution of β-catenin.RESULTS: parenchymal tumors were palpable in the abdomens of nude mice 2 wk post-implantation and orthotopic tumor formation rate was 100% in all groups. 5-Fu treatment alone significantly decreased tumor weight compared to the CON group(1.203 ± 0.284 g vs 1.804 ± 0.649 g, p < 0.01). WCA treatment alone reduced the rate of metastasis(50% vs 100%,p < 0.05). Combination treatment of WCA + 5-Fu was the most effective, reducing the tumor weight(1.140 ± 0.464 g vs 1.804 ± 0.649 g, p < 0.01) and size(1493.438 ± 740.906 mm3 vs 2180.259 ± 816.556 mm3, p < 0.05), the rate of metastases(40% vs 100%, p < 0.01), and serum CEA levels(31.263 ± 7.421 μg/L vs 43.040 ± 11.273 μg/L, p < 0.05). Expression of β-catenin and MMp-7 was decreased in drug-treated groups compared to controls, as detected using realtime quantitative RT-p CR, immunohistochemistry and immunoblotting, respectively. Cell fractionation assays revealed that nuclear translocation of β-catenin was reduced by WCA and/or 5-Fu treatments. CONCLUSION: Combination of WCA with 5-Fu significantly inhibited colon tumor growth and hepatic metastases. Decreased expression of β-catenin and MMp-7 may be important.展开更多
OBJECTIVE:To systematically evaluate the efficacy and safety of Weichang’an pill(胃肠安丸,WCA)combined with Western Medicine(WM)for the treatment of gastrointestinal diseases.METHODS:Eight databases,including China N...OBJECTIVE:To systematically evaluate the efficacy and safety of Weichang’an pill(胃肠安丸,WCA)combined with Western Medicine(WM)for the treatment of gastrointestinal diseases.METHODS:Eight databases,including China National Knowledge Infrastructure Database,Wanfang Data,China Science and Technology Journal Database,SinoMed,PubMed,Web of Science,Cochrane Library,and Embase,were searched for randomized controlled trials(RCTs)of WCA from inception to 30 September 2021.We independently screened the literature,extracted data,and then evaluated the bias risk,effectiveness,safety,and other indicators of the included articles.RESULTS:A total of 33 RCTs were included in this study with 3368 patients.After analysis,it was found that WCA combined with WM could effectively prevent and treat antibiotic-associated gastrointestinal reaction,functional dyspepsia(FD),irritable bowel syndrome,rotavirus diarrhea(RVD),and ulcerative colitis(UC);no serious adverse reactions occurred.Moreover,compared with the control group,the experimental group showed significantly improved symptoms and some biochemical parameters.CONCLUSIONS:WCA combined with WM for the treatment of gastrointestinal diseases had better clinical efficacy than the control group,without serious adverse reactions.Notably,in the treatment of FD,RVD,and UC,WCA improved clinical symptoms and biochemical indicator expression.Nevertheless,owing to the restricted quality and quantity of the literature,the results need to be further studied using high-quality RCTs.展开更多
This paper describes the fossil fruits of Scirpus weichangensis X.Q.Liang,sp.nov.from the early Miocene of Guangyongfa Village,Weichang County,Hebei Province,North China.The fossil fruits are obovate in shape and thei...This paper describes the fossil fruits of Scirpus weichangensis X.Q.Liang,sp.nov.from the early Miocene of Guangyongfa Village,Weichang County,Hebei Province,North China.The fossil fruits are obovate in shape and their lateral sections are plumply trigonous.The cell walls of the surface are straight.The persistent stout bristles have downward-directed barbellae in distal 1/2.The occurrence of the fossil Scirpus indicates that Guangfayong was a wetland in the early Miocene.Based on the fossil data,the genus likely originated in Western Siberia in the Oligocene,spread during the Miocene,and was finally distributed worldwide in the Holocene.展开更多
Spirematospermum is a well-known extinct zingiberalean taxon, characterized by trilocular capsules containing many distinctive, spirally striate and arillate seeds. It is frequently found and studied in European Neoge...Spirematospermum is a well-known extinct zingiberalean taxon, characterized by trilocular capsules containing many distinctive, spirally striate and arillate seeds. It is frequently found and studied in European Neogene carpological floras, but is scarcely represented in East Asia floras. In this work we recognize a new fossil record of Spirematospermum wetzleri（Heer） Chandler based on the capsules and seeds from the Miocene of Weichang, Hebei Province, North China. These fossils represent the first record of the species in the Miocene of China. Fossil data indicate that Spirematospermum probably originated in the Late Cretaceous of North America or Central Europe.The genus still existed in the Paleocene of North America, but became extinct after that time. However, the genus successively survived in Europe from the Eocene to Pliocene, and flourished luxuriantly during the Oligocene to Miocene. As there was Turgai Strait between Europe and Asia during the Eocene, the genus did not spread to Central Asia and West Siberia until the strait closed in the late Eocene/early Oligocene, and further expanded eastwardly to eastern Siberia, Russia, northern China and central Japan during the Miocene, but became extinct in Asia after the Miocene. The genus contracted its distribution to Europe in the Pliocene, and afterwards it became extinct in the world.展开更多
目的研究胃肠安丸(Weichang′an pill,WCA)、胃肠安丸醇提物(Ethanol extract of Weichang′an pill,EE)、胃肠安丸水提物(Water extract of Weichang′an pill,WE)及其活性成分对乙酰胆碱(Acetylcholine,ACh)诱导的大鼠离体回肠平滑肌...目的研究胃肠安丸(Weichang′an pill,WCA)、胃肠安丸醇提物(Ethanol extract of Weichang′an pill,EE)、胃肠安丸水提物(Water extract of Weichang′an pill,WE)及其活性成分对乙酰胆碱(Acetylcholine,ACh)诱导的大鼠离体回肠平滑肌收缩的影响及机制。方法采用离体组织浴实验,在ACh的作用下,加入WCA、EE、WE或其活性成分,记录离体大鼠回肠平滑肌的收缩张力;通过分子对接的方法探究活性成分与毒蕈碱型乙酰胆碱M3受体的结合亲和力。结果WCA、EE、WE均可明显抑制ACh诱导的回肠平滑肌兴奋性收缩。活性成分木香烃内酯、去氢木香内酯、檀香醇、麝香酮、大黄素、大黄酚、大黄素甲醚、巴豆苷、厚朴酚及和厚朴酚也对ACh诱导的回肠平滑肌收缩有明显的抑制作用。结论WCA、EE、WE及其活性成分可能通过阻断回肠平滑肌细胞膜上的M3受体与ACh的结合,发挥促进肠平滑肌松弛的作用。展开更多
基金Supported by Natural Science Research Fund of Longhua Hospital Affiliated to Shanghai University of Traditional Chinese MedicineBudgetary Scientific Research Project of the Education Commission of Shanghai"The impact of Weichang’an decoction onβ-catenin/MMP7 signaling pathway in nude mice with hepatic metastasis from colorectal cancer:a study on the molecular mechanism",No.2011JW33Young Talent Scientific Research Project of Shanghai Municipal Commission of Health and Family Planning"Efficacy Evaluation of combined therapy with TCM and western medicine for the treatment of hepatic metastasis from unresectable colorectal cancer",No.20134y141
文摘AIM: To examine the effect of Weichang'an(WCA) and 5-fluorouracil(5-Fu) on colorectal tumor and hepatic metastasis in a mouse model. METHODS: Quantitative determination of hesperidin, the effective component in WCA decoction, was performed using Hp LC. In vitro cytotoxicity of WCA was determined by treating HCT-116 cells with WCA diluents or serum extracted from rats that received WCA by oral gavage for 1 wk and MTT assays. Forty-eight nude mice received cecum implantation with tumor blocks subcutaneously amplified from human colon cancer cell line HCT-116. Mice were randomly divided into four treatment groups: control(CON), WCA, 5-Fu and combination(WCA + 5-Fu). pathological examination of tumors consisted of tissue sectioning and hematoxylin and eosin staining. Tumor weight and size were measured, and the number of metastatic l e s i o n s wa s c o u n t e d. S e r u m c a r c i n o e m b r yo n i c antigen(CEA) level was determined by ELISA. The expression levels of tumor genesis and metastasisrelated proteins β-catenin and matrix metalloproteinase(MMp)-7 were measured by real-time quantitative reverse transcriptase polymerase chain reaction(RTp CR), immunohistochemistry and immunoblotting. Cell fractionation was used to investigate intracellular distribution of β-catenin.RESULTS: parenchymal tumors were palpable in the abdomens of nude mice 2 wk post-implantation and orthotopic tumor formation rate was 100% in all groups. 5-Fu treatment alone significantly decreased tumor weight compared to the CON group(1.203 ± 0.284 g vs 1.804 ± 0.649 g, p < 0.01). WCA treatment alone reduced the rate of metastasis(50% vs 100%,p < 0.05). Combination treatment of WCA + 5-Fu was the most effective, reducing the tumor weight(1.140 ± 0.464 g vs 1.804 ± 0.649 g, p < 0.01) and size(1493.438 ± 740.906 mm3 vs 2180.259 ± 816.556 mm3, p < 0.05), the rate of metastases(40% vs 100%, p < 0.01), and serum CEA levels(31.263 ± 7.421 μg/L vs 43.040 ± 11.273 μg/L, p < 0.05). Expression of β-catenin and MMp-7 was decreased in drug-treated groups compared to controls, as detected using realtime quantitative RT-p CR, immunohistochemistry and immunoblotting, respectively. Cell fractionation assays revealed that nuclear translocation of β-catenin was reduced by WCA and/or 5-Fu treatments. CONCLUSION: Combination of WCA with 5-Fu significantly inhibited colon tumor growth and hepatic metastases. Decreased expression of β-catenin and MMp-7 may be important.
基金Training Project of Innovation Team of Colleges and Universities in Tianjin:Study on Compound Chinese Medicine (Grant No.TD13-5053)Scientific Research Project of Tianjin Educational Committee:Research on the Basic Biology of the Wind Drugs Effects of “Dissipate Wind to Dissipate Heat,Dispel Wind to Relieve Pain” via TLR4/NF-κB Signalling Pathway (Grant No.2018KJ007)+2 种基金Innovation Team and Talents Cultivation Program of National Administration of Traditional Chinese Medicine (Grant No.ZYYCXTD-C-202009)National Natural Science Foundation of China:Research on the Therapeutic Effect and Mechanism of “Wind Dispeling” Herb Vitex Trifolia for Migraine Treatment via CGRP/ERK/NF-κB/NLRP3 Signaling Pathway (No.82004331)National Natural Science Foundation of China:Research on the Effects of Erzhi pills via 11β-HSD1-GC/GR Pathway on Cognitive Function and Its Mechanism of Chronic Mild and Unpredictable Stress Depression Mice (No.81973792)
文摘OBJECTIVE:To systematically evaluate the efficacy and safety of Weichang’an pill(胃肠安丸,WCA)combined with Western Medicine(WM)for the treatment of gastrointestinal diseases.METHODS:Eight databases,including China National Knowledge Infrastructure Database,Wanfang Data,China Science and Technology Journal Database,SinoMed,PubMed,Web of Science,Cochrane Library,and Embase,were searched for randomized controlled trials(RCTs)of WCA from inception to 30 September 2021.We independently screened the literature,extracted data,and then evaluated the bias risk,effectiveness,safety,and other indicators of the included articles.RESULTS:A total of 33 RCTs were included in this study with 3368 patients.After analysis,it was found that WCA combined with WM could effectively prevent and treat antibiotic-associated gastrointestinal reaction,functional dyspepsia(FD),irritable bowel syndrome,rotavirus diarrhea(RVD),and ulcerative colitis(UC);no serious adverse reactions occurred.Moreover,compared with the control group,the experimental group showed significantly improved symptoms and some biochemical parameters.CONCLUSIONS:WCA combined with WM for the treatment of gastrointestinal diseases had better clinical efficacy than the control group,without serious adverse reactions.Notably,in the treatment of FD,RVD,and UC,WCA improved clinical symptoms and biochemical indicator expression.Nevertheless,owing to the restricted quality and quantity of the literature,the results need to be further studied using high-quality RCTs.
基金supported by grants from the National Natural Science Foundation of China(Nos.31760057,31860049,41502017,41072022)
文摘This paper describes the fossil fruits of Scirpus weichangensis X.Q.Liang,sp.nov.from the early Miocene of Guangyongfa Village,Weichang County,Hebei Province,North China.The fossil fruits are obovate in shape and their lateral sections are plumply trigonous.The cell walls of the surface are straight.The persistent stout bristles have downward-directed barbellae in distal 1/2.The occurrence of the fossil Scirpus indicates that Guangfayong was a wetland in the early Miocene.Based on the fossil data,the genus likely originated in Western Siberia in the Oligocene,spread during the Miocene,and was finally distributed worldwide in the Holocene.
基金supported by the Natural Science Foundation of China (Grants No. 41502017 and No. 41072022)the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDPB05)
文摘Spirematospermum is a well-known extinct zingiberalean taxon, characterized by trilocular capsules containing many distinctive, spirally striate and arillate seeds. It is frequently found and studied in European Neogene carpological floras, but is scarcely represented in East Asia floras. In this work we recognize a new fossil record of Spirematospermum wetzleri（Heer） Chandler based on the capsules and seeds from the Miocene of Weichang, Hebei Province, North China. These fossils represent the first record of the species in the Miocene of China. Fossil data indicate that Spirematospermum probably originated in the Late Cretaceous of North America or Central Europe.The genus still existed in the Paleocene of North America, but became extinct after that time. However, the genus successively survived in Europe from the Eocene to Pliocene, and flourished luxuriantly during the Oligocene to Miocene. As there was Turgai Strait between Europe and Asia during the Eocene, the genus did not spread to Central Asia and West Siberia until the strait closed in the late Eocene/early Oligocene, and further expanded eastwardly to eastern Siberia, Russia, northern China and central Japan during the Miocene, but became extinct in Asia after the Miocene. The genus contracted its distribution to Europe in the Pliocene, and afterwards it became extinct in the world.
文摘目的研究胃肠安丸(Weichang′an pill,WCA)、胃肠安丸醇提物(Ethanol extract of Weichang′an pill,EE)、胃肠安丸水提物(Water extract of Weichang′an pill,WE)及其活性成分对乙酰胆碱(Acetylcholine,ACh)诱导的大鼠离体回肠平滑肌收缩的影响及机制。方法采用离体组织浴实验,在ACh的作用下,加入WCA、EE、WE或其活性成分,记录离体大鼠回肠平滑肌的收缩张力;通过分子对接的方法探究活性成分与毒蕈碱型乙酰胆碱M3受体的结合亲和力。结果WCA、EE、WE均可明显抑制ACh诱导的回肠平滑肌兴奋性收缩。活性成分木香烃内酯、去氢木香内酯、檀香醇、麝香酮、大黄素、大黄酚、大黄素甲醚、巴豆苷、厚朴酚及和厚朴酚也对ACh诱导的回肠平滑肌收缩有明显的抑制作用。结论WCA、EE、WE及其活性成分可能通过阻断回肠平滑肌细胞膜上的M3受体与ACh的结合,发挥促进肠平滑肌松弛的作用。
