In terms of special geological conditions of the Western Route of South-to-North Water Transfer Project, the classification method for surrounding rocks is discussed by combining with the construction method of tunnel...In terms of special geological conditions of the Western Route of South-to-North Water Transfer Project, the classification method for surrounding rocks is discussed by combining with the construction method of tunnel boring machine （TBM）. The classification standard of surrounding rocks is put forward on the basis of physical simulations and engineering practices. Damage, deformation and evolution of surrounding rocks induced by TBM excavation are discussed. Meanwhile, the long-term deformation mechanisms and stability of surrounding rocks are also studied. On this basis, a three-dimensional constitutive model for interbedded sandstone slate and a fiat shell-joint element-foundation system for calculating internal forces of segment lining are established. The deformation features of surrounding rocks of deep and steep interbedded sandstone slate and their influences on internal forces of segment lining are presented. Finally, the design methods of segment lining constructed in deep and steep flysch are proposed.展开更多
It is well known that there is abundant water resources in basin of the Yangtze River, the first largest river in China, which is mainly located in Southern China. However, water resources is very scarce in the basin ...It is well known that there is abundant water resources in basin of the Yangtze River, the first largest river in China, which is mainly located in Southern China. However, water resources is very scarce in the basin of the Yellow River, which is mainly located in Northern China. So the western route project of south-north water transfer scheme （WRP-SNWTS） aims to transfer water from the Yangtze River to the Yellow River. The area of WRP-SNWT, located in the upper reaches of the Yangtze River and the main areas of Sichuan and the marginal areas of the Qinghai-Tibet Plateau, has sufficient water resources but fragile ecology and environment. Therefore, it is necessary for WRP-SNWT to analyze the ecology water required. Based on the planning principles of from low elevation to high elevation, from small to large, from short to long and from easy to difficulty, the WRP-SNWT will be constructed through three stage projects. The western route first stage project of the south-north water transfer scheme （WRFST-SNWTS） is planned to transfer 4×10^9m^3/a from six tributaries of the Yalong river and from Dadu river to Jiaqu of Yellow River.. Daqu river and Niqu river are the branches of Xianshui river. Sequ river, Duke river, Make river and Ake river are the branches of Dadu river, which account for 65-70% of the total river run-off. It need more research and the rest run-off can satisfy channel ecology water required. According to analysis ecological water required which mainly satisfy for aquicolous biology in water-exporting region, such as low air temperature. Fish and aquicolous biology main living from May to August, and rivers are iced up from December to March of next year, ecology water required mainly for fish and aquicolous biology. The flow criterion of Tennant method is modified. The ecology water required of WRFSP-SNWTS is estimated by the flow data of Zhuwo gauging station, Zhuba gauging station, Chuosijia gauging station and Zumuzu gauging station. The result show that the ecology water required calculated by modified Tennant less 1 l percent than that of Tennant. This estimating result can supply more water resources for transferring to Yellow River. Meanwhile, this can supply gist for research transferring water of WRFSP-SNWTS.展开更多
In this paper, a two-region endogenous growth model is adapted to analyse Western China's development,and regional divergence and convergence processes. Dynamic optimization is utilized to calculate the growth rat...In this paper, a two-region endogenous growth model is adapted to analyse Western China's development,and regional divergence and convergence processes. Dynamic optimization is utilized to calculate the growth rate of the Chinese economy on the optimal growth path, and backward integration is utilized to simulate the evolvement of some key indicators, including relative GDP per capita of Western China to Eastern China, regional capital growth rate and regional GDP growth rate. The calibrations of this model after 1992 are conducted. The calibration results provide evidence about the speed of Western China's relative development, and also shed some light on the future regional convergence process under the Western Development Programme launched in 2000.展开更多
Western blotting is a popular technique for examining expression levels of proteins using gel-based electrophoretic fractionation followed by blotting and antibody reactions. Although this is a mature technique, one o...Western blotting is a popular technique for examining expression levels of proteins using gel-based electrophoretic fractionation followed by blotting and antibody reactions. Although this is a mature technique, one of the major limitations is the need to prepare an individual electrophoretic gel for each of the protein species to be analyzed. Since most analyses require the detection of multiple protein species, a procedure that allows utilization of a single gel for detecting multiple protein species should significantly save time and resources. In this paper, we developed a novel multiprotein detection device, which enabled simultaneous detection of several proteins species from a single electrophoretic gel. In this device, a protein transfer unit utilized a multi-anode plate that generated a non-uniform voltage profile. This voltage profile enabled uniform transfer regardless of molecular mass of proteins. In vitro experiments using samples, isolated from boneforming osteoblast cells, showed that the expression levels of 5 - 7 different proteins were detectable in the presence and absence of mechanical stimulation that activated genes necessary for bone formation. The result supports the notion that through simultaneous detection of multiple protein species, the described device contributes to reduction in procedural time and sample amounts, as well as a removal of variations among multiple gels.展开更多
BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs ...BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.展开更多
The Western Route of the South-to-North Water Transfer Project will divert water from the upper Yangtze River and its tributaries, the Dadu River and Yalong River, to the upper Yellow River. The project may ease the w...The Western Route of the South-to-North Water Transfer Project will divert water from the upper Yangtze River and its tributaries, the Dadu River and Yalong River, to the upper Yellow River. The project may ease the water shortage in the Yellow River Basin. However, it may also have some effects on the ecosystem in the upper Yangtze River Basin. Benthic invertebrates play an important role in the river ecosystem, particularly in the circulation of materials and nutrition. Benthic invertebrates are widely used to quickly assess river ecosystems because of their rapid response to changes in the water environment. The diversity of benthic invertebrates is closely associated with the aquatic habitat area. This study examined this interaction by sampling the benthic invertebrates in an expanding area. The conclusions are that the diversity of benthic invertebrates begins to decrease when the aquatic habitat area is reduced to 45% of the original area, and decreases dramatically when the aquatic habitat area is reduced to 10% of the original area. The aquatic habitat area should be kept at more than 45% of the original area in order to maintain the significant diversity of benthic invertebrates.展开更多
With the depth development of economic globalization, the multi-culture conflict, communication and integration are strengthened. Meanwhile, series of problems about cross-cultural communication for international busi...With the depth development of economic globalization, the multi-culture conflict, communication and integration are strengthened. Meanwhile, series of problems about cross-cultural communication for international business have happened. One of the core problem is negative cultural transfer. This paper gives the analysis about its causes and effects. At last, the way to solve it have been found.展开更多
基金Supported by the National Key Technology R&D Program in the 11th Five-year Plan of China (2006BAB04A06)
文摘In terms of special geological conditions of the Western Route of South-to-North Water Transfer Project, the classification method for surrounding rocks is discussed by combining with the construction method of tunnel boring machine （TBM）. The classification standard of surrounding rocks is put forward on the basis of physical simulations and engineering practices. Damage, deformation and evolution of surrounding rocks induced by TBM excavation are discussed. Meanwhile, the long-term deformation mechanisms and stability of surrounding rocks are also studied. On this basis, a three-dimensional constitutive model for interbedded sandstone slate and a fiat shell-joint element-foundation system for calculating internal forces of segment lining are established. The deformation features of surrounding rocks of deep and steep interbedded sandstone slate and their influences on internal forces of segment lining are presented. Finally, the design methods of segment lining constructed in deep and steep flysch are proposed.
文摘It is well known that there is abundant water resources in basin of the Yangtze River, the first largest river in China, which is mainly located in Southern China. However, water resources is very scarce in the basin of the Yellow River, which is mainly located in Northern China. So the western route project of south-north water transfer scheme （WRP-SNWTS） aims to transfer water from the Yangtze River to the Yellow River. The area of WRP-SNWT, located in the upper reaches of the Yangtze River and the main areas of Sichuan and the marginal areas of the Qinghai-Tibet Plateau, has sufficient water resources but fragile ecology and environment. Therefore, it is necessary for WRP-SNWT to analyze the ecology water required. Based on the planning principles of from low elevation to high elevation, from small to large, from short to long and from easy to difficulty, the WRP-SNWT will be constructed through three stage projects. The western route first stage project of the south-north water transfer scheme （WRFST-SNWTS） is planned to transfer 4×10^9m^3/a from six tributaries of the Yalong river and from Dadu river to Jiaqu of Yellow River.. Daqu river and Niqu river are the branches of Xianshui river. Sequ river, Duke river, Make river and Ake river are the branches of Dadu river, which account for 65-70% of the total river run-off. It need more research and the rest run-off can satisfy channel ecology water required. According to analysis ecological water required which mainly satisfy for aquicolous biology in water-exporting region, such as low air temperature. Fish and aquicolous biology main living from May to August, and rivers are iced up from December to March of next year, ecology water required mainly for fish and aquicolous biology. The flow criterion of Tennant method is modified. The ecology water required of WRFSP-SNWTS is estimated by the flow data of Zhuwo gauging station, Zhuba gauging station, Chuosijia gauging station and Zumuzu gauging station. The result show that the ecology water required calculated by modified Tennant less 1 l percent than that of Tennant. This estimating result can supply more water resources for transferring to Yellow River. Meanwhile, this can supply gist for research transferring water of WRFSP-SNWTS.
基金Supported by the National Natural Science Foundation of China(No.71403015)
文摘In this paper, a two-region endogenous growth model is adapted to analyse Western China's development,and regional divergence and convergence processes. Dynamic optimization is utilized to calculate the growth rate of the Chinese economy on the optimal growth path, and backward integration is utilized to simulate the evolvement of some key indicators, including relative GDP per capita of Western China to Eastern China, regional capital growth rate and regional GDP growth rate. The calibrations of this model after 1992 are conducted. The calibration results provide evidence about the speed of Western China's relative development, and also shed some light on the future regional convergence process under the Western Development Programme launched in 2000.
文摘Western blotting is a popular technique for examining expression levels of proteins using gel-based electrophoretic fractionation followed by blotting and antibody reactions. Although this is a mature technique, one of the major limitations is the need to prepare an individual electrophoretic gel for each of the protein species to be analyzed. Since most analyses require the detection of multiple protein species, a procedure that allows utilization of a single gel for detecting multiple protein species should significantly save time and resources. In this paper, we developed a novel multiprotein detection device, which enabled simultaneous detection of several proteins species from a single electrophoretic gel. In this device, a protein transfer unit utilized a multi-anode plate that generated a non-uniform voltage profile. This voltage profile enabled uniform transfer regardless of molecular mass of proteins. In vitro experiments using samples, isolated from boneforming osteoblast cells, showed that the expression levels of 5 - 7 different proteins were detectable in the presence and absence of mechanical stimulation that activated genes necessary for bone formation. The result supports the notion that through simultaneous detection of multiple protein species, the described device contributes to reduction in procedural time and sample amounts, as well as a removal of variations among multiple gels.
文摘BlotMan is a protein blotting device that allows generating multiple membranes from a single polyacrylamide gel. To transfer all proteins uniformly with the same efficiency regardless of protein size, BlotMan employs pulse-width-modulated (PWM) voltage that applies a higher average voltage to a larger protein species. BlotMan can be controlled not only by its custom-made interface but also by a smart phone via Bluetooth technology. In this study, we examined effects of PWM signals (50%, 60%, and 80% duty cycle) on transfer efficiency and signal intensity in comparison to a constant voltage signal (100% duty cycle). The result revealed that in response to the same average voltage of 150 V, a lower duty cycle with a higher maximum voltage increased transfer efficiency as well as sharpness of transferred proteins. We validated BlotMan’s capability using a chondrosarcoma cell line (SW1353 cells) and a breast cancer cell line (MDA-MB231 cells) in response to antitumor chemical agents. BlotMan successfully generated 5 membranes from a single gel and detected 5 protein species such as c-Src, eukaryotic translation initiation factor 2 alpha (eIF2), phosphorylated eIF2, lamin B, and actin. Collectively, we demonstrated herein that BlotMan reduces an amount of protein samples by generating multiple membranes from a single gel and improving signal intensity with PWM voltage signals.
基金supported by the National Hi-Tech Research and Development Program of China ("863" Project) (Grant No. 2006BAB04A08)the National Natural Science Foundation of China (Grant No. 50779027)
文摘The Western Route of the South-to-North Water Transfer Project will divert water from the upper Yangtze River and its tributaries, the Dadu River and Yalong River, to the upper Yellow River. The project may ease the water shortage in the Yellow River Basin. However, it may also have some effects on the ecosystem in the upper Yangtze River Basin. Benthic invertebrates play an important role in the river ecosystem, particularly in the circulation of materials and nutrition. Benthic invertebrates are widely used to quickly assess river ecosystems because of their rapid response to changes in the water environment. The diversity of benthic invertebrates is closely associated with the aquatic habitat area. This study examined this interaction by sampling the benthic invertebrates in an expanding area. The conclusions are that the diversity of benthic invertebrates begins to decrease when the aquatic habitat area is reduced to 45% of the original area, and decreases dramatically when the aquatic habitat area is reduced to 10% of the original area. The aquatic habitat area should be kept at more than 45% of the original area in order to maintain the significant diversity of benthic invertebrates.
文摘With the depth development of economic globalization, the multi-culture conflict, communication and integration are strengthened. Meanwhile, series of problems about cross-cultural communication for international business have happened. One of the core problem is negative cultural transfer. This paper gives the analysis about its causes and effects. At last, the way to solve it have been found.
