Utility of fluid inclusion paleo-temperature in petroleum system modelling:A case study from western offshore,India

The paleo-temperature(Th)data from fluid inclusions are utilized for thermal history modelling using PetroMod software.Generally,bottom hole temperature(BHT)and vitrinite reflectance(Ro)measurements are widely used in petroleum system modelling(PSM)in the oil industry for calibration purposes.Th representing the minimum temperature of fluid entrapment estimated from fluid-inclusion study provides extra support to build the thermal models for PSM.Fluid inclusion parameters along with Rock-Eval pyrolysis analysis have been used to predict the maturity of oil in terms of API gravity as well as the maturity of source rocks respectively.Two exploratory wells RV-1(Mumbai Offshore Basin)and KK4C-A-1(Kerala-Konkan Offshore Basin),India were examined and the T_(h)from most of the fluid inclusions of wells RV-1 and KK4C-A-1 fell in the oil window range of 60-140℃suggesting thermal conditions favourable for oil generation in both of the wells.T_(h)of coeval aqueous inclusions along with the Hydrocarbon Fluid inclusions(HCFIs)was used to calibrate PSM.Vital parameters show that source rocks of well RV-1 are mature and that of well KK4C-A-1 are immature.Two sets of PSM are created in terms of generation and expulsion for the dry wells RV-1 and KK4C-A-1 and calibrated each well using fluid inclusion Th and BHT.From the fluid inclusion analysis method,it is evident that hydrocarbon generation happened in both wells and the paleo-temperature indicates that the formations of both wells were subjected to temperatures in the oil window range,even though it was designated as dry wells in the present scenario.The present study highlights the application of fluid inclusion paleo-temperature(Th)during calibration instead of commonly used methods.We could obtain desirable and accurate data output from PSM using T_(h) calibration.

基于网络药理学和大鼠体内验证的藏药红景天改善脑微循环障碍作用机制研究

目的基于文献研究、网络药理学、分子对接与实验验证的方法,探究藏药红景天改善脑微循环障碍的核心靶点、关键成分和作用机制。方法通过文献和数据库收集红景天化学成分,利用反向药效团匹配预测红景天的潜在靶点;获取脑微循环障碍靶点,并与红景天靶点映射,构建交集靶点蛋白互作网络,获取核心靶点;构建“中药-成分-核心靶点-疾病”调控网络并获取关键成分;进行GO和KEGG富集分析,构建“核心靶点-信号通路-生物过程”网络;进行分子对接验证;采用RT-qPCR和Western blot进行动物实验验证,进一步证实网络药理学分析结果。结果从红景天中筛选出76个活性成分和285个靶点,获取脑微循环障碍靶点1074个,交集靶点97个,核心靶点6个,关键成分6个。分子对接结果表明,有3个关键成分与核心靶点的结合性大于核心靶点蛋白与其原始配体结合性。RT-qPCR结果显示红景天能下调核心靶点CASP3、AKT1 mRNA水平,Western blot检测结果显示藏药红景天能降低CASP3、AKT1蛋白表达(P<0.05)。结论藏药红景天可通过多成分、多靶点、多通路协同改善脑微循环障碍,该研究为临床应用藏药红景天治疗脑微循环障碍提供实验依据。

Efficiency of Integrated Traditional Chinese Medicine and Western Medicine on Outcomes in Patients with Sepsis: A Systematic Review and Meta-Analysis

Background:Sepsis is a life-threatening disease with high mortality and a poor prognosis,partly due to a lack of effective treatments.Therefore,more effective treatments are urgently needed.Methods:Eight databases were searched for use of traditional Chinese medicine(TCM) for sepsis.A meta-analysis was performed using Stata 15.0 software.The 28-day mortality,length of hospital stay,and Acute Physiology and Chronic Health Evaluation Ⅱ(APACHE-Ⅱ) score were the outcomes of this study.The Cochrane Collaboration tool for assessing risk of bias was used for quality assessment.Results:Ten studies with a total of 847 patients were included in this meta-analysis.Compared with the control group,the experimental group had lower 28-day mortality(relative risk [RR] 0.67;95% confidence interval [CI] 0.53 to 0.84;P=0.001),shorter hospital stay(weighted mean difference [WMD]-2.17;95% CI:-3.2 to -1.14;P=0.000) and lower APACHE-Ⅱ score(WMD-2.164;95% CI:-3.236 to -1.091;P=0.000).Conclusion:Our results demonstrate that TCM together with standard Western medicine was associated with significantly lower 28-day mortality,APACHE Ⅱ scores,and shorter hospital intensive care unit stays,suggesting that TCM might be an effective alternative treatment for sepsis.

Photosystem Development in Dark-grown Lotus (Nelumbo nucifera) Seedlings

It is well known that no chlorophyll synthesis and photosystem biogenesis have been detected in dark-grown angiosperm seedlings. However, in this report, we showed that both PS II and PS I could be formed in dark-grown lotus (Nelumbo nucifera Gaertn.) seedlings. Lots of evidence were given: First I during the dark-grown period, the single fluorescence emission peak at 679 nm in lotus embryo red-shifted and transformed into the normal PS II fluorescence emission; Simultaneously, PS I fluorescence emission at 730 nm appeared and increased obviously; Second, with partial denaturing SDS-PAGE method, PS I chlorophyll-protein complex could be clearly separated from 10 days dark-grown lotus seedlings; Third, the existence of Lhca1 was also proved by Western blots. Moreover, measurements of electron transfer rate demonstrated that both PS II and PS I core in dark-grown lotus seedlings were photochemically active.

A Physics-informed Deep-learning Intensity Prediction Scheme for Tropical Cyclones over the Western North Pacific

Accurate prediction of tropical cyclone(TC)intensity is challenging due to the complex physical processes involved.Here,we introduce a new TC intensity prediction scheme for the western North Pacific(WNP)based on a time-dependent theory of TC intensification,termed the energetically based dynamical system(EBDS)model,together with the use of a long short-term memory(LSTM)neural network.In time-dependent theory,TC intensity change is controlled by both the internal dynamics of the TC system and various environmental factors,expressed as environmental dynamical efficiency.The LSTM neural network is used to predict the environmental dynamical efficiency in the EBDS model trained using besttrack TC data and global reanalysis data during 1982–2017.The transfer learning and ensemble methods are used to retrain the scheme using the environmental factors predicted by the Global Forecast System(GFS)of the National Centers for Environmental Prediction during 2017–21.The predicted environmental dynamical efficiency is finally iterated into the EBDS equations to predict TC intensity.The new scheme is evaluated for TC intensity prediction using both reanalysis data and the GFS prediction data.The intensity prediction by the new scheme shows better skill than the official prediction from the China Meteorological Administration(CMA)and those by other state-of-art statistical and dynamical forecast systems,except for the 72-h forecast.Particularly at the longer lead times of 96 h and 120 h,the new scheme has smaller forecast errors,with a more than 30%improvement over the official forecasts.

Quantitative effect of kerogen type on the hydrocarbon generation potential of Paleogene lacustrine source rocks,Liaohe Western Depression,China

Kerogen types exert a decisive effect on the onset and capacity of hydrocarbon generation of source rocks.Lacustrine source rocks in the Liaohe Western Depression are characterized by thick deposition,high total organic carbon(TOC)content,various kerogen types,and a wide range of thermal maturity.Consequently,their hydrocarbon generation potential and resource estimation can be misinterpreted.In this study,geochemical tests,numerical analysis,hydrocarbon generation kinetics,and basin modeling were integrated to investigate the differential effects of kerogen types on the hydrocarbon generation potential of lacustrine source rocks.Optimized hydrocarbon generation and expulsion(HGE)models of different kerogen types were established quantitatively upon abundant Rock-Eval/TOC/vitrinite reflectance(R_(o))datasets.Three sets of good-excellent source rocks deposited in the fourth(Es4),third(Es3),and first(Es1)members of Paleogene Shahejie Formation,are predominantly types I-II_(1),II_(1)-II_(2),and II-III,respectively.The activation energy of types I-II_(2)kerogen is concentrated(180-230 kcal/mol),whereas that of type III kerogen is widely distributed(150-280 kcal/mol).The original hydrocarbon generation potentials of types I,II_(1),II_(2),and III kerogens are 790,510,270,and 85 mg/g TOC,respectively.The Ro values of the hydrocarbon generation threshold for type I-III source rocks gradually increase from 0.42%to 0.74%,and Ro values of the hydrocarbon expulsion threshold increase from 0.49%to 0.87%.Types I and II_(1)source rocks are characterized by earlier hydrocarbon generation,more rapid hydrocarbon expulsion,and narrower hydrocarbon generation windows than types II_(2)and III source rocks.The kerogen types also affect the HGE history and resource potential.Three types(conventional,tight,and shale oil/gas)and three levels(realistic,expected,and prospective)of hydrocarbon resources of different members in the Liaohe Western Depression are evaluated.Findings suggest that the Es3 member has considerable conventional and unconventional hydrocarbon resources.This study can quantitatively characterize the hydrocarbon generation potential of source rocks with different kerogen types,and facilitate a quick and accurate assessment of hydrocarbon resources,providing strategies for future oil and gas exploration.

CREB1和CREB3在胃癌及癌前病变组织中的表达及其临床意义

目的探究环磷腺苷反应元件结合蛋白1(CREB1)和环磷腺苷反应元件结合蛋白3(CREB3)在胃癌及癌前病变组织中的表达及临床意义。方法收集慢性浅表性胃炎40例,慢性萎缩性胃炎伴肠化生40例,异型增生40例,胃癌50例,采用免疫组化法检测CREB1和CREB3在四种组织中的表达,并分析其与临床病理参数的关系。另收集同期新鲜组织慢性浅表性胃炎、慢性萎缩性胃炎伴肠化生、胃癌共30例,应用蛋白印迹法(Western blot)检测CREB1和CREB3在不同胃组织中的表达。利用Kaplan-Meier plotter分析CREB1和CREB3的表达与胃癌患者总生存时间(OS)和无进展生存时间(FP)的相关性。利用STRING数据库分析CREB1和CREB3在信号通路中的位置以及与之相关的上下游基因。结果免疫组化显示:胃癌组、异型增生组、萎缩性胃炎伴肠化生组CREB1、CREB3阳性表达率明显高于慢性浅表性胃炎组(P<0.05);并且CREB1、CREB3在胃癌组的阳性表达率明显高于慢性萎缩性胃炎伴肠化生组(P<0.05),但与异型增生组无统计学差异(P>0.05)。临床病理参数分析得出两者表达水平与浸润深度、TNM分期、脉管侵犯、淋巴结转移相关(P<0.05);与患者性别、年龄、肿瘤大小、分化程度无明显相关性(P>0.05)。Western blot结果显示:CREB1、CREB3蛋白表达在慢性浅表性胃炎、慢性萎缩性胃炎伴肠化生、胃癌中依次增高,差异有统计学意义(P<0.01)。Kaplan-Meier plotter分析显示CREB1、CREB3高表达的胃癌患者OS和FP均缩短。结论CREB1、CREB3可能与胃癌的发生、发展与转移相关,且蛋白高表达可能与胃癌患者预后不良有关。

Natural gas exploration potential and favorable targets of Permian Fengcheng Formation in the western Central Depression of Junggar Basin,NW China

Based on the organic geochemical data and the molecular and stable carbon isotopic compositions of natural gas of the Lower Permian Fengcheng Formation in the western Central Depression of Junggar Basin,combined with sedimentary environment analysis and hydrocarbon-generating simulation,the gas-generating potential of the Fengcheng source rock is evaluated,the distribution of large-scale effective source kitchen is described,the genetic types of natural gas are clarified,and four types of favorable exploration targets are selected.The results show that:(1)The Fengcheng Formation is a set of oil-prone source rocks,and the retained liquid hydrocarbon is conducive to late cracking into gas,with characteristics of high gas-generating potential and late accumulation;(2)The maximum thickness of Fengcheng source rock reaches 900 m.The source rock has entered the main gas-generating stage in Penyijingxi and Shawan sags,and the area with gas-generating intensity greater than 20×10^(8) m^(3)/km^(2) is approximately 6500 km^(2).(3)Around the western Central Depression,highly mature oil-type gas with light carbon isotope composition was identified to be derived from the Fengcheng source rocks mainly,while the rest was coal-derived gas from the Carboniferous source rock;(4)Four types of favorable exploration targets with exploration potential were developed in the western Central Depression which are structural traps neighboring to the source,stratigraphic traps neighboring to the source,shale-gas type within the source,and structural traps within the source.Great attention should be paid to these targets.

Differences in spring precipitation over southern China associated with multiyear La Ni?a events

Composite analyses were performed in this study to reveal the difference in spring precipitation over southern China during multiyear La Ni?a events during 1901 to 2015. It was found that there is significantly below-normal precipitation during the first boreal spring, but above-normal precipitation during the second year. The difference in spring precipitation over southern China is correlative to the variation in western North Pacific anomalous cyclone(WNPC), which can in turn be attributed to the different sea surface temperature anomaly(SSTA) over the Tropical Pacific. The remote forcing of negative SSTA in the equatorial central and eastern Pacific and the local air-sea interaction in the western North Pacific are the usual causes of WNPC formation and maintenance.SSTA in the first spring is stronger than those in the second spring. As a result, the intensity of WNPC in the first year is stronger, which is more likely to reduce the moisture in southern China by changing the moisture transport, leading to prolonged precipitation deficits over southern China. However, the tropical SSTA signals in the second year are too weak to induce the formation and maintenance of WNPC and the below-normal precipitation over southern China. Thus, the variation in tropical SSTA signals between two consecutive springs during multiyear La Ni?a events leads to obvious differences in the spatial pattern of precipitation anomaly in southern China by causing the different WNPC response.

基于特征多肽抗原的阿胶基原鉴定

目的 以特征多肽为半抗原并制备多克隆抗体,鉴定阿胶原材料基原。方法 经文献调研及数据库比对筛选特征多肽,将多肽抗原与血蓝蛋白偶联并免疫Balb/c小鼠制备多克隆抗体,通过ELISA、dot blot和Western blot分析与验证多克隆抗体的产生、效价及特异性。结果 筛选所得5个特征多肽序列均具有T细胞表位和B细胞表位,亲水性-1~0,二级结构大多为无规则卷曲。多肽抗原ECS1、ECS3~ECS5在二次加强免疫后,效价分别为1∶6 400、1∶400、1∶12 800和1∶800。制备所得的多克隆抗体之间不产生交叉反应,而且多克隆抗体Ab-ECS3与驴皮及其伪品蛋白的结合显现出较大差异。结论 以特征多肽为半抗原制备所得的多克隆抗体可通过Western blot对驴皮进行真伪鉴别,为胶类中药的基原鉴定提供了参考。

SIRT6在胃癌中的表达及临床意义

目的探讨沉默信息调节因子6(Sirtuin 6,SIRT6)在胃癌组织中的表达及临床意义。方法生物信息学分析SIRT6 mRNA在胃癌中的表达及其与生存、肿瘤突变负荷(TMB)及TIDE评分的关系;收集95例胃癌及癌旁组织标本,免疫组织化学方法检测SIRT6的表达水平,分析其与临床病理特征的关系;Western blot法检测10例新鲜胃癌及癌旁组织中SIRT6的表达情况。结果生物信息学分析显示,SIRT6 mRNA在胃癌中表达高于正常胃组织(P<0.05),Kaplan-Meier生存曲线显示SIRT6高表达组生存时间较短,突变相关分析及TIDE评分显示SIRT6高表达组具有更高的TMB及TIDE评分(P<0.05),且SIRT6 mRNA表达水平与TMB及TIDE评分呈显著正相关(P<0.05)。免疫组织化学结果显示SIRT6在胃癌组织中表达率为85.2%(81/95);SIRT6在胃癌中高表达与高Ki-67增殖指数相关,差异具有统计学意义(P<0.05),SIRT6表达在不同年龄、性别、肿瘤直径及P53等组间差异均无统计学意义(P>0.05);Western blot显示胃癌组织中SIRT6的表达水平高于癌旁组织(9/10),差异具有统计学意义(P<0.05)。结论SIRT6在胃癌组织中高表达,可能通过影响基因组稳定性及胃癌细胞增殖能力参与胃癌的发生发展。

Geomorphic signatures and active tectonics in western Saurashtra,Gujarat,India

Active tectonics in an area includes ongoing or recent geologic events.This paper investigates the tectonic influence on the subsidence,uplift and tilt of western Saurashtra through morphotectonic analysis of ten watersheds along with characteristics of relief and drainage orientation.Watersheds 7-9 to the north(N)are tectonically active,which can be linked with the North Kathiawar Fault System(NKFS)and followed by watersheds 6,10,1,4 and 5.Stream-length gradient index and sinuosity index indicate the effect of tectonic events along the master streams in watersheds 6-9.Higher R^(2)values of the linear curve fit for watershed 7 indicate its master stream is much more tectonically active than the others.The R^(2)curve fitting model and earthquake magnitude/depth analysis confirm the region to be active.The reactivation of the NKFS most likely led to the vertical movement of western Saurashtra.

Climate warming is significantly influenced by rising summer maximum temperatures: insights from tree-ring evidence of the Western Tianshan Mountains, China

As one of the regions most affected by global cli-mate warming,the Tianshan mountains has experienced sev-eral ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is considered mainly to be caused by increases in minimum temperatures and winter temperatures,while the influence of maximum temperatures is unclear.In this study,a 300-year tree-ring chronology developed from the Western Tianshan Moun-tains was used to reconstruct the summer(June-August)maximum temperature(T_(max6-8))variations from 1718 to 2017.The reconstruction explained 53.1% of the variance in the observed T_(max6-8).Over the past 300 years,the T_(max6-8)reconstruction showed clear interannual and decadal vari-abilities.There was a significant warming trend(0.18°C/decade)after the 1950s,which was close to the increasing rates of the minimum and mean temperatures.The increase in maximum temperature was also present over the whole Tianshan mountains and its impact on climate warming has increased.The T_(max6-8) variations in the Western Tianshan mountains were influenced by frequent volcanic eruptions combined with the influence of solar activity and the sum-mer North Atlantic Oscillation.This study reveals that cli-mate warming is significantly influenced by the increase in maximum temperatures and clarifies possible driving mech-anisms of temperature variations in the Western Tianshan mountains which should aid climate predictions.

Climate warming is significantly influenced by rising summer maximum temperatures:insights from tree-ring evidence of the Western Tianshan Mountains,China

As one of the regions most affected by global climate warming,the Tianshan mountains has experienced several ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is considered mainly to be caused by increases in minimum temperatures and winter temperatures,while the influence of maximum temperatures is unclear.In this study,a 300-year tree-ring chronology developed from the Western Tianshan Mountains was used to reconstruct the summer(June-August)maximum temperature(Tmax6-8) variations from 1718 to2017.The reconstruction explained 53.1% of the variance in the observed Tmax6-8.Over the past 300 years,the Tmax6-8reconstruction showed clear interannual and decadal variabilities.There was a significant warming trend(0.18 ℃/decade) after the 1950s,which was close to the increasing rates of the minimum and mean temperatures.The increase in maximum temperature was also present over the whole Tianshan mountains and its impact on climate warming has increased.The Tmax6-8variations in the Western Tianshan mountains were influenced by frequent volcanic eruptions combined with the influence of solar activity and the summer North Atlantic Oscillation.This study reveals that climate warming is significantly influenced by the increase in maximum temperatures and clarifies possible driving mechanisms of temperature variations in the Western Tianshan mountains which should aid climate predictions.

Impacts of hydropower-induced flow alterations on composition and diversity of riparian vegetation in the Western Himalayas: A case study in Uttarakhand, India

The increasing demand for water and energy resources has led to widespread dam construction,particularly in ecologically sensitive regions like the Himalayan Range.This study focuses on the Uttarakhand state in the Western Himalayas,where hydroelectric projects(HEPs)have significantly altered river flow regimes.The research investigates the impact of flow alterations on the composition and structure of riparian vegetation in the Garhwal Himalayas,specifically analysing four rivers regulated by hydroelectric projects.Utilizing the paired-reach comparison method,control(undisturbed),diverted(downstream of barrage/dam),and altered flow conditions(downstream of water outlet)were examined.The research reveals diverse and unique riparian ecosystems,with 89 genera and 113 taxa identified,showcasing the dominance of families like Asteraceae and Lamiaceae.The study unveils the structural importance of key species such as Berberis asiatica and Artemisia nilagirica.The density,diversity,and richness of shrub and herb species vary significantly across flow conditions.Notably,altered flow conditions demonstrate resilience in vegetation structure,while diverted conditions exhibit decreased species richness and density.The study emphasizes the importance of nuanced environmental flow management for mitigating adverse effects on riparian biodiversity in the fragile Himalayan region.These findings contribute to the global discourse on dam impacts and riparian ecology,shedding light on the complexities of this dynamic relationship in a vulnerable ecosystem.

Research on the development methodology for clinical practice guidelines for organic integration of traditional Chinese medicine and Western medicine

Integrated traditional Chinese medicine(TCM)and Western medicine(WM)is a new medical science grounded in the knowledge bases of both TCM and WM,which then forms a unique modern medical system in China.Integrated TCM and WM has a long history in China,and has made important achievements in the process of clinical diagnosis and treatment.However,the methodological defects in currently published clinical practice guidelines(CPGs)limit its development.The organic integration of TCM and WM is a deeper integration of TCM and WM.To realize the progression of"integration"to"organic integration",a targeted and standardized guideline development methodology is needed.Therefore,the purpose of this study is to establish a standardized development procedure for clinical practice guidelines for the organic integration of TCM and WM to promote the systematic integration of TCM and WM research results into clinical practice guidelines in order to achieve optimal results as the whole is greater than the sum of the parts.

Environmental Conditions Conducive to the Formation of Multiple Tropical Cyclones over the Western North Pacific

There is limited understanding regarding the formation of multiple tropical cyclones(MTCs).This study explores the environmental conditions conducive to MTC formation by objectively determining the atmospheric circulation patterns favorable for MTC formation over the western North Pacific.Based on 199 MTC events occurring from June to October 1980–2020,four distinct circulation patterns are identified:the monsoon trough(MT)pattern,accounting for 40.3%of occurrences,the confluence zone(CON)pattern at 26.2%,the easterly wave(EW)pattern at 17.8%,and the monsoon gyre(MG)pattern at 15.7%.The MT pattern mainly arises from the interaction between the subtropical high and the monsoon trough,with MTCs forming along the monsoon trough and its flanks.The CON pattern is affected by the subtropical high,the South Asian high,and the monsoon trough,with MTCs emerging at the confluence zone where the prevailing southwesterly and southeasterly flows converge.The EW pattern is dominated by easterly flows,with MTCs developing along the easterly wave train.MTCs in the MG pattern arise within a monsoon vortex characterized by strong southwesterly flows.A quantitative analysis further indicates that MTC formation in the MT pattern is primarily governed by mid-level vertical velocity and low-level vorticity,while mid-level humidity and vertical velocity are significantly important in the other patterns.The meridional shear and convergence of zonal winds are essential in converting barotropic energy from the basic flows to disturbance kinetic energy,acting as the primary source for eddy kinetic energy growth.

Track-Pattern-Based Characteristics of Extratropical Transitioning Tropical Cyclones in the Western North Pacific

Based on the Regional Specialized Meteorological Center(RSMC)Tokyo-Typhoon Center best-track data and the NCEP-NCAR reanalysis dataset,extratropical transitioning(ET)tropical cyclones(ETCs)over the western North Pacific(WNP)during 1951–2021 are classified into six clusters using the fuzzy c-means clustering method(FCM)according to their track patterns.The characteristics of the six hard-clustered ETCs with the highest membership coefficient are shown.Most tropical cyclones(TCs)that were assigned to clusters C2,C5,and C6 made landfall over eastern Asian countries,which severely threatened these regions.Among landfalling TCs,93.2%completed their ET after landfall,whereas 39.8%of ETCs completed their transition within one day.The frequency of ETCs over the WNP has decreased in the past four decades,wherein cluster C5 demonstrated a significant decrease on both interannual and interdecadal timescales with the expansion and intensification of the western Pacific subtropical high(WPSH).This large-scale circulation pattern is favorable for C2 and causes it to become the dominant track pattern,owning to it containing the largest number of intensifying ETCs among the six clusters,a number that has increased insignificantly over the past four decades.The surface roughness variation and three-dimensional background circulation led to C5 containing the maximum number of landfalling TCs and a minimum number of intensifying ETCs.Our results will facilitate a better understanding of the spatiotemporal distributions of ET events and associated environment background fields,which will benefit the effective monitoring of these events over the WNP.

Old-growth mixed dipterocarp forests show variable losses and gains in aboveground biomass and standing carbon over forty years

Background:No studies have documented long-term trends in aboveground biomass(AGB)for mixed-dipterocarp forests(MDF),the dominant rain forest type in tropical wet equatorial Asia.In our study,we sought to document such trends over forty years across three sites representing lowland to lower montane elevations.Methods:To do this,we established fifty 100 m×25 m plots in 1978 across three sites sampled along an elevation gradient,identified as mature old-growth forest.We measured trees for diameter at breast height that we identified to species and tagged.We took wood samples to calculate species wood-specific gravity.We re-measured plots in 1998 and again in 2018.Results:We show standing AGB for all sites combined to be 517.52 Mg·ha^(-1)in 1978,but this declined by 17%over 40 years to 430.11 Mg·ha^(-1).No differences exist among sites in AGB primarily because of considerable within site variation;but interactions of time with site show declines across sites were not uniform,one remained about the same.Relatively few species represented a high proportion of the AGB with the top five species comprising between 34%and 65%,depending upon site and year sampled.One species,Mesua nagassarium,represented a disproportionately large amount of AGB and decline over time,particularly at the low elevation site.Conclusions:Our results are directly relevant to estimating AGB and standing carbon sequestered in MDF.Our study is the first to demonstrate varying but overall,declining trends in amounts of AGB among forests making predictions of biomass and standing carbon in MDF difficult over wide regions.

Population genetic insights into the conservation of common walnut(Juglans regia)in Central Asia

The common walnut(Juglans regia)is one of the most economically important nut trees cultivated worldwide.Despite its importance,no comprehensive evaluation of walnut tree population genetics has been undertaken across the range where it originated,Central Asia.In this study,we investigated the genetic diversity and population structure of 1082 individuals from 46 populations across Central Asia.We found moderate genetic diversity of J.regia across Central Asia,with 46 populations clustered into three groups with a weak relationship between genetic and geographic distance.Our findings reveal that the western Himalaya might be the core region of common walnut genetic diversity in Central Asia and that,except for two populations in Gongliu Wild Walnut Valley,humans might have introduced walnut populations to Xinjiang,China.The observed distribution of the genetic landscape has probably been affected by historical climate fluctuation,breeding system,and prolonged anthropogenic activity.We propose the conservation of the core genetic diversity resources in the western Himalaya and pay special attention to populations from Gongliu in Xinjiang.These findings enhance our understanding of the genetic variation throughout the distribution range of J.regia in Central Asia,which will provide a key prerequisite for evidence-based conservation and management.