As an important factor that directly affects agricultural production, the social economy, and policy implementation,observed changes in dry/wet conditions have become a matter of widespread concern. However, previous ...As an important factor that directly affects agricultural production, the social economy, and policy implementation,observed changes in dry/wet conditions have become a matter of widespread concern. However, previous research has mainly focused on the long-term linear changes of dry/wet conditions, while the detection and evolution of the non-linear trends related to dry/wet changes have received less attention. The non-linear trends of the annual aridity index, obtained by the Ensemble Empirical Mode Decomposition(EEMD) method, reveal that changes in dry/wet conditions in China are asymmetric and can be characterized by contrasting features in both time and space in China. Spatially, most areas in western China have experienced transitions from drying to wetting, while opposite changes have occurred in most areas of eastern China. Temporally, the transitions occurred earlier in western China compared to eastern China. Research into the asymmetric spatial characteristics of dry/wet conditions compensates for the inadequacies of previous studies, which focused solely on temporal evolution;at the same time, it remedies the inadequacies of traditional research on linear trends over centennial timescales. Analyzing the non-linear trend also provides for a more comprehensive understanding of the drying/wetting changes in China.展开更多
Ecological stability is a core issue in ecological research and holds significant implications forhumanity. The increased frequency and intensity of drought and wet climate events resulting from climatechange pose a m...Ecological stability is a core issue in ecological research and holds significant implications forhumanity. The increased frequency and intensity of drought and wet climate events resulting from climatechange pose a major threat to global ecological stability. Variations in stability among different ecosystemshave been confirmed, but it remains unclear whether there are differences in stability within the sameterrestrial vegetation ecosystem under the influence of climate events in different directions and intensities.China's grassland ecosystem includes most grassland types and is a good choice for studying this issue.This study used the Standardized Precipitation Evapotranspiration Index-12 (SPEI-12) to identify thedirections and intensities of different types of climate events, and based on Normalized DifferenceVegetation Index (NDVI), calculated the resistance and resilience of different grassland types for 30consecutive years from 1990 to 2019 (resistance and resilience are important indicators to measurestability). Based on a traditional regression model, standardized methods were integrated to analyze theimpacts of the intensity and duration of drought and wet events on vegetation stability. The resultsshowed that meadow steppe exhibited the highest stability, while alpine steppe and desert steppe had thelowest overall stability. The stability of typical steppe, alpine meadow, temperate meadow was at anintermediate level. Regarding the impact of the duration and intensity of climate events on vegetationecosystem stability for the same grassland type, the resilience of desert steppe during drought was mainlyaffected by the duration. In contrast, the impact of intensity was not significant. However, alpine steppewas mainly affected by intensity in wet environments, and duration had no significant impact. Ourconclusions can provide decision support for the future grassland ecosystem governance.展开更多
Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breedin...Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG.展开更多
In this study,the perovskite nanocomposite PrFe_(x)Co_(1-x)O_(3)(Pr(S))was successfully synthesized by the sol-gel method;PrFe_(x)Co_(1-x)O_(3)/Al-pillared montmorillonite(Pr(S)/Mt)catalysts were prepared by impregnat...In this study,the perovskite nanocomposite PrFe_(x)Co_(1-x)O_(3)(Pr(S))was successfully synthesized by the sol-gel method;PrFe_(x)Co_(1-x)O_(3)/Al-pillared montmorillonite(Pr(S)/Mt)catalysts were prepared by impregnation(D)method and solid-melting(G)method,respectively,with Pr(S)as the active component and Al-pillared montmorillonite as the carrier.The catalysts were applied to treat the 2-hydroxybenzoic acid(2-HA)-simulated wastewater by catalytic wet peroxide oxidation(CWPO)technique,and the chemical oxygen demand(COD)removal rate and the 2-HA degradation rate were used as indicators to evaluate the catalytic performance.The results of the experiment indicated that the solid-melting method was more conducive to preparing the catalyst when the Co/Fe molar ratio of 7:3 and the optimal structural properties of the catalysts were achieved.The influence of operating parameters,including reaction temperature,catalyst dosage,H_(2)O_(2)dosage,pH,and initial 2-HA concentration,were optimized for the degradation of 2-HA by CWPO.The results showed that 97.64%of 2-HA degradation and 75.23%of COD removal rate were achieved under more suitable experimental conditions.In addition,after the catalyst was used five times,the degradation rate of 2-HA could still reach 76.93%,which implied the high stability and reusability of the catalyst.The high catalytic activity of the catalyst was due to the doping of Co into PrFeO_(3),which could promote the generation of HO·,and the high stability could be attributed to the loading of Pr(S)onto Al-Mt,which reduced the leaching of reactive metals.The study of reaction mechanism and kinetics showed that the whole degradation process conformed to the pseudo-firstorder kinetic equation,and the Langmuir-Hinshelwood method was applied to demonstrate that catalysis was dominant in the degradation process.展开更多
The traditional standard wet sieving method uses steel sieves with aperture?0.063 mm and can only determine the particle size distribution(PSD)of gravel and sand in general soil.This paper extends the traditional meth...The traditional standard wet sieving method uses steel sieves with aperture?0.063 mm and can only determine the particle size distribution(PSD)of gravel and sand in general soil.This paper extends the traditional method and presents an extended wet sieving method.The extended method uses both the steel sieves and the nylon filter cloth sieves.The apertures of the cloth sieves are smaller than 0.063 mm and equal 0.048 mm,0.038 mm,0.014 mm,0.012 mm,0.0063 mm,0.004 mm,0.003 mm,0.002 mm,and 0.001 mm,respectively.The extended method uses five steps to separate the general soil into many material sub-groups of gravel,sand,silt and clay with known particle size ranges.The complete PSD of the general soil is then calculated from the dry masses of the individual material sub-groups.The extended method is demonstrated with a general soil of completely decomposed granite(CDG)in Hong Kong,China.The silt and clay materials with different particle size ranges are further examined,checked and verified using stereomicroscopic observation,physical and chemical property tests.The results further confirm the correctness of the extended wet sieving method.展开更多
●AIM:To determine the teaching effects of a real-time three dimensional(3D)visualization system in the operating room for early-stage phacoemulsification training.●METHODS:A total of 10 ophthalmology residents of th...●AIM:To determine the teaching effects of a real-time three dimensional(3D)visualization system in the operating room for early-stage phacoemulsification training.●METHODS:A total of 10 ophthalmology residents of the first-year postgraduate were included.All the residents were novices to cataract surgery.Real-time cataract surgical observations were performed using a custom-built 3D visualization system.The training lasted 4wk(32h)in all.A modified International Council of Ophthalmology’s Ophthalmology Surgical Competency Assessment Rubric(ICO-OSCAR)containing 4 specific steps of cataract surgery was applied.The self-assessment(self)and expert-assessment(expert)were performed through the microsurgical attempts in the wet lab for each participant.●RESULTS:Compared with pre-training assessments(self 3.2±0.8,expert 2.5±0.6),the overall mean scores of posttraining(self 5.2±0.4,expert 4.7±0.6)were significantly improved after real-time observation training of 3D visualization system(P<0.05).Scores of 4 surgical items were significantly improved both self and expert assessment after training(P<0.05).●CONCLUSION:The 3D observation training provides novice ophthalmic residents with a better understanding of intraocular microsurgical techniques.It is a useful tool to improve teaching efficiency of surgical education.展开更多
Integrated data and energy transfer(IDET)enables the electromagnetic waves to transmit wireless energy at the same time of data delivery for lowpower devices.In this paper,an energy harvesting modulation(EHM)assisted ...Integrated data and energy transfer(IDET)enables the electromagnetic waves to transmit wireless energy at the same time of data delivery for lowpower devices.In this paper,an energy harvesting modulation(EHM)assisted multi-user IDET system is studied,where all the received signals at the users are exploited for energy harvesting without the degradation of wireless data transfer(WDT)performance.The joint IDET performance is then analysed theoretically by conceiving a practical time-dependent wireless channel.With the aid of the AO based algorithm,the average effective data rate among users are maximized by ensuring the BER and the wireless energy transfer(WET)performance.Simulation results validate and evaluate the IDET performance of the EHM assisted system,which also demonstrates that the optimal number of user clusters and IDET time slots should be allocated,in order to improve the WET and WDT performance.展开更多
The main soil type, principle contributor of nutrients and available agricultural land in the Hula Valley is the organic Peat. Nevertheless, the relative contribution of Phosphorus from the Hula Valley to the Lake Kin...The main soil type, principle contributor of nutrients and available agricultural land in the Hula Valley is the organic Peat. Nevertheless, the relative contribution of Phosphorus from the Hula Valley to the Lake Kinneret inputs is lower than regional outsourcing. The Nitrogenic matter, mostly Nitrate migration from the Peat soil is significant. The implementation of efficient development is the key factor of Hula Land use. The financial beneficial success of the Hula land use is therefore dependent of Peat soil properties. The porosity of the Peat Soil is high and preferential pathway volume is low and Hydraulic Conductivity is therefore low. Consequently, the Mobile Spray Irrigation line was found as most suitable for cultivation in Peat Soil. Enhancement of Summer irrigation creating moisture elevation reduces Phosphorus migration from Peat Soil and is therefore recommended and recently implemented.展开更多
Cell-free(CF)multiple-input multiple-output(MIMO)is a promising technique to enable the vision of ubiquitous wireless connectivity for next-generation network communications.Compared to traditional co-located massive ...Cell-free(CF)multiple-input multiple-output(MIMO)is a promising technique to enable the vision of ubiquitous wireless connectivity for next-generation network communications.Compared to traditional co-located massive MIMO,CF MIMO allows geographically distributed access points(APs)to serve all users on the same time-frequency resource with spatial multiplexing techniques,resulting in better performance in terms of both spectral efficiency and coverage enhancement.However,the performance gain is achieved at the expense of deploying more APs with high cost and power consumption.To address this issue,the recently proposed reconfigurable intelligent surface(RIS)technique stands out with its unique advantages of low cost,low energy consumption and programmability.In this paper,we provide an overview of RIS-assisted CF MIMO and its interaction with advanced optimization designs and novel applications.Particularly,recent studies on typical performance metrics such as energy efficiency(EE)and spectral efficiency(SE)are surveyed.Besides,the application of RIS-assisted CF MIMO techniques in various future communication systems is also envisioned.Additionally,we briefly discuss the technical challenges and open problems for this area to inspire research direction and fully exploit its potential in meeting the demands of future wireless communication systems.展开更多
Introduction:Cupping therapy is an integrative and complementary medicine therapy that practiced worldwide by several civilizations for thousands of years.Cupping therapy is reported to be a relatively safe practice e...Introduction:Cupping therapy is an integrative and complementary medicine therapy that practiced worldwide by several civilizations for thousands of years.Cupping therapy is reported to be a relatively safe practice especially if performed by qualified therapists.Subconjunctival hemorrhage(SCH)is a common cause of eye redness and can be classified into traumatic and spontaneous.Case presentation:This case report discussed an adverse event related to wet cupping therapy.The patient developed SCH one day after performing cupping therapy.He was completely recovered after 2 weeks.This case report should increase the knowledge of cupping therapists regarding increasing safety of patient when performing wet cupping therapy on specific body parts especially,head,neck and cervical areas.Furthermore,it highlighted the importance of conducting research regarding the mechanical and physiological effects of cupping therapy.Conclusion:Adverse events related to cupping therapy should be reported with every patient.Cupping therapists should be aware of this possible mild adverse event.To the best of our knowledge,this is the first published adult case of SCH related to wet cupping therapy session.More studies are critically needed for evaluation of cupping therapy effects and adverse events especially when performed on head and neck.展开更多
The present investigation focuses on the thermal performance of a fully wet stretching/shrinking longitudinal fin of exponential profile coated with a mechanism like a conveyer belt.The modeled equation is non-dimensi...The present investigation focuses on the thermal performance of a fully wet stretching/shrinking longitudinal fin of exponential profile coated with a mechanism like a conveyer belt.The modeled equation is non-dimensionalized and solved by applying the Runge-Kutta-Fehlberg(RKF)method.The effects of parameters such as the wet parameter,the fin shape parameter,and the stretching/shrinking parameter on the heat transfer and thermal characteristics of the fin are graphically analyzed and discussed.It is inferred that the negative effects of motion and internal heat generation on the fin heat transfer rate can be lessened by setting a shrinking mechanism on the fin surface.The current examination is inclined towards practical applications and is beneficial to the design of fins.展开更多
Extensive dust control on the dry Owens Lake mainly uses constructed basins that are flooded with shallow depths of fresh water. This dust control is mandated by law as a minimum percent of the area of each individual...Extensive dust control on the dry Owens Lake mainly uses constructed basins that are flooded with shallow depths of fresh water. This dust control is mandated by law as a minimum percent of the area of each individual wetting basin. Wetted surfaces are evaluated for area and degree of wetness using the shortwave infrared (SWIR) band of Landsat TM, or similar earth observation satellite sensor. The SWIR region appropriate for these measurements lies within the electromagnetic spectrum between about 1.5 and 1.8 μm wavelengths. A threshold value for Landsat TM5 band 5 reflectance of 0.19 was found to conform with surfaces having a threshold for adequate wetting at a nascent point where rapid drying would occur following loss of capillary connection with groundwater. This threshold is robust and requires no atmospheric correction for the effects of aerosol scatter and attenuation as long as the features on the image appear clear. Monthly monitoring of surface wetting has proven accurate, verifiable and repeatable using these methods. This threshold can be calibrated for any Earth observation satellite that records the appropriate SWIR region. The monitoring program is expected to provide major input for the final phase of the dust control program that will have a focus to conserve water and resources.展开更多
Water–rock interaction(WRI)is a topic of interest in geology and geotechnical engineering.Many geological hazards and engineering safety problems are severe under the WRI.This study focuses on the water weakening of ...Water–rock interaction(WRI)is a topic of interest in geology and geotechnical engineering.Many geological hazards and engineering safety problems are severe under the WRI.This study focuses on the water weakening of rock strength and its infuencing factors(water content,immersion time,and wetting–drying cycles).The strength of the rock mass decreases to varying degrees with water content,immersion time,and wetting–drying cycles depending on the rock mass type and mineral composition.The corresponding acoustic emission count and intensity and infrared radiation intensity also weaken accordingly.WRI enhances the plasticity of rock mass and reduces its brittleness.Various microscopic methods for studying the pore characterization and weakening mechanism of the WRI were compared and analyzed.Various methods should be adopted to study the pore evolution of WRI comprehensively.Microscopic methods are used to study the weakening mechanism of WRI.In future work,the mechanical parameters of rocks weakened under long-term water immersion(over years)should be considered,and more attention should be paid to how the laboratory scale is applied to the engineering scale.展开更多
Heat events may be humid or dry.While several indices incorporate humidity,such combined indices obscure identification and exploration of heat events by their different humidity characteristics.The new HadISDH.extrem...Heat events may be humid or dry.While several indices incorporate humidity,such combined indices obscure identification and exploration of heat events by their different humidity characteristics.The new HadISDH.extremes global gridded monitoring product uniquely provides a range of wet and dry bulb temperature extremes indices.Analysis of this new data product demonstrates its value as a tool for quantifying exposure to humid verses dry heat events.It also enables exploration into“stealth heat events”,where humidity is high,perhaps enough to affect productivity and health,while temperature remains moderate.Such events may not typically be identified as“heat events”by temperature-focused heat indices.Over 1973-2022,the peak magnitude of humid extremes(maximum daily wet bulb temperature over a month;T_(w)X)for the global annual mean increased significantly at 0.13±0.04℃(10 yr)^(−1),which is slightly slower than the global annual mean T_(w) increase of 0.22±0.04℃(10 yr)^(−1).The frequency of moderate humid extreme events per year(90th per-centile daily maxima wet bulb temperature exceedance;T_(w)X90p)also increased significantly at 4.61±1.07 d yr^(−1)(10 yr)^(−1).These rates were slower than for temperature extremes,TX and TX90p,which respectively increased significantly at 0.27±0.04℃(10 yr)^(−1) and 5.53±0.72 d yr^(−1)(10 yr)^(−1).Similarly,for the UK/Europe focus region,JJA-mean T_(w)X increased significantly,again at a slower rate than for TX and mean T_(w).HadISDH.extremes shows some evidence of“stealth heat events”occurring where humidity is high but temperature remains more moderate.展开更多
HadISDH.extremes is an annually updated global gridded monthly monitoring product of wet and dry bulb temperature–based extremes indices,from January 1973 to December 2022.Data quality,including spatial and temporal ...HadISDH.extremes is an annually updated global gridded monthly monitoring product of wet and dry bulb temperature–based extremes indices,from January 1973 to December 2022.Data quality,including spatial and temporal stability,is a key focus.The hourly data are quality controlled.Homogeneity is assessed on monthly means and used to score each gridbox according to its homogeneity rather than to apply adjustments.This enables user-specific screening for temporal stability and avoids errors from inferring adjustments from monthly means for the daily maximum values.For general use,a score(HQ Flag)of 0 to 6 is recommended.A range of indices are presented,aligning with existing standardised indices.Uniquely,provision of both wet and dry bulb indices allows exploration of heat event character—whether it is a“humid and hot”,“dry and hot”or“humid and warm”event.It is designed for analysis of long-term trends in regional features.HadISDH.extremes can be used to study local events,but given the greater vulnerability to errors of maximum compared to mean values,cross-validation with independent information is advised.展开更多
The wide engineered application of compacted expansive soils necessitates understanding their behavior under field conditions.The results of this study demonstrate how seasonal climatic variation and stress and bounda...The wide engineered application of compacted expansive soils necessitates understanding their behavior under field conditions.The results of this study demonstrate how seasonal climatic variation and stress and boundary conditions individually or collectively influence the hydraulic and volume change behavior of compacted highly expansive soils.The cyclic wetting and drying(CWD)process was applied for two boundary conditions,i.e.constant stress(CS)and constant volume(CV),and for a wide range of axial stress states.The adopted CWD process affected the hydraulic and volume change behaviors of expansive soils,with the first cycle of wetting and drying being the most effective.The CWD process under CS conditions resulted in shrinkage accumulation and reduction in saturated hydraulic conductivity(k sat).On the other hand,CWD under CV conditions caused a reduction of swell pressure while has almost no impact on k sat.An elastic response to CWD was achieved after the third cycle for saturated hydraulic conductivity(k sat),the third to fourth cycle for the volume change potential under the CV conditions,and the fourth to fifth cycle for the volume change potential under the CS conditions.Finally,both swell pressure(s s)and saturated hydraulic conductivity(k sat)are not fundamental parameters of the expansive soil but rather depend on stress,boundary and wetting conditions.展开更多
The objective of this study was to analyze the response of runoff in the area of runoff yield of the upstream Shiyang River basin to climate change and to promote sustainable development of regional water resources an...The objective of this study was to analyze the response of runoff in the area of runoff yield of the upstream Shiyang River basin to climate change and to promote sustainable development of regional water resources and ecological environment.As the biggest tributary of the Shiyang River,Xiying River is the only hydrological station(Jiutiaoling) that has provincial natural river and can achieve long time series monitoring data in the basin.The data obtained from this station is representative of natural conditions because it has little human activites.This study built a regression model through identifying the characteristics of runoff and climate change by using Mann-Kendall nonparametric statistical test,cumulative anomaly,and correlation analysis.The results show that the average annual runoff is 320.6 million m3/a with the coefficient of variation of 0.18 and shows slightly decrease during 1956-2020.It has a significant positive correlation the average annual precipitation(P<0.01).Runoff is sensitive to climate change,and the climate has becoming warm and wet and annual runoff has entering wet period from 2003.Compared to the earlier period(1955-2000),the increases of average annual temperature,precipitation and runoff in recent two decades were 15%,9.3%,and 7.8%,respectively.Runoff in the Shiyang River is affected by temperature and precipitation among climate factors,and the simulation results of the runoff-climate response model(R=0.0052P-0.1589T+2.373) indicate that higher temperature leads to a weakening of the ecological regulation of surface runoff in the flow-producing area.展开更多
Wellbore instability,especially drilling with water-based drilling fluids(WBDFs)in complex shale for-mations,is a critical challenge for oil and gas development.The purpose of this paper is to study the feasibility of...Wellbore instability,especially drilling with water-based drilling fluids(WBDFs)in complex shale for-mations,is a critical challenge for oil and gas development.The purpose of this paper is to study the feasibility of using hydrophobically modified silica nanoparticle(HMN)to enhance the comprehensive performance of WBDFs in the Xinjiang Oilfield,especially the anti-collapse performance.The effect of HMN on the overall performance of WBDFs in the Xinjiang Oilfield,including inhibition,plugging,lu-bricity,rheology,and filtration loss,was studied with a series of experiments.The mechanism of HMN action was studied by analyzing the changes of shale surface structure and chemical groups,wettability,and capillary force.The experimental results showed that HMN could improve the performance of WBDFs in the Xinjiang Oilfeld to inhibit the hydration swelling and dispersion of shale.The plugging and lubrication performance of the WBDFs in the Xinjiang Oilfield were also enhanced with HMN based on the experimental results.HMN had less impact on the rheological and filtration performance of the WBDFs in the Xinjiang Oilfield.In addition,HMN significantly prevented the decrease of shale strength.The potential mechanism of HMN was as follows.The chemical composition and structure of the shale surface were altered due to the adsorption of HMN driven by electrostatic attraction.Changes of the shale surface resulted in significant wettability transition.The capillary force of the shale was converted from a driving force of water into the interior to a resistance.In summary,hydrophobic nanoparticles presented afavorable application potential for WBDFs.展开更多
Inflatable wing has significant application value in the design of loitering munitions because of its advantages such as lightweight and foldability.However,due to the flexible characteristics,aeroelastic behaviors of...Inflatable wing has significant application value in the design of loitering munitions because of its advantages such as lightweight and foldability.However,due to the flexible characteristics,aeroelastic behaviors of inflatable wings such as flutter are nonnegligible in flight.By designing a certain angle between the inflatable beam and the wing span,the structural dynamic and even the aeroelastic performance of the inflatable wing can be effectively improved.Based on the analysis of the mechanical and geometric characteristics of the inflatable structure,a new inflatable wing with sweep arranged inflatable beams is proposed,and the main design variables and methods are analyzed.For purpose of investigating the aeroelastic performance of the swept baffled inflatable wing,the modal behaviors by considering the wet mode are studied.In consideration of the deficiencies of the traditional wet modal analysis method,by introducing the influence on the additional stiffness of flow field,an added massstiffness method is proposed in this paper,and the advantages are verified by ground vibration experiments.On this basis,the effects of baffles sweep angle,pressure,and boundary conditions on the modal parameters and aeroelastic performance of inflatable wing are analyzed.The results show that the aeroelastic performance of the inflatable wing can be designed by changing the baffles sweep angle,which is enlightened for the aeroelastic tailoring design on inflatable wings.展开更多
Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was propo...Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was proposed to utilize water and nutrients more sustainable.In this study,we selected six rice cultivars(Centauro,Loto,Selenio,Vialone nano,JSendra and Puntal)grown under AWD conditions,and investigated their responsiveness to AM colonization and how they select diverse AM taxa.In order to investigate root-associated AM fungus communities,molecular cloning-Sanger sequencing on small subunit rDNA data were obtained from five out of the six rice cultivars and compared with Next Generation Sequencing(NGS)data,which were previously obtained in Vialone nano.The results showed that all the cultivars were responsive to AM colonization with the development of AM symbiotic structures,even if with differences in the colonization and arbuscule abundance in the root systems.We identified 16 virtual taxa(VT)in the soil compartment and 7 VT in the root apparatus.We emphasized that the NGS analysis gives additional value to the results thanks to a more in-depth reading of the less represented AM fungus taxa.展开更多
基金supported by the National key research and development program (2019YFA0607104)National Natural Science Foundation of China (Grant Nos. 41991231, 42275034, 41975076, 42075029, 42075017, and 42075018)the Gansu Provincial Science and Technology Project (22JR5RA405)。
文摘As an important factor that directly affects agricultural production, the social economy, and policy implementation,observed changes in dry/wet conditions have become a matter of widespread concern. However, previous research has mainly focused on the long-term linear changes of dry/wet conditions, while the detection and evolution of the non-linear trends related to dry/wet changes have received less attention. The non-linear trends of the annual aridity index, obtained by the Ensemble Empirical Mode Decomposition(EEMD) method, reveal that changes in dry/wet conditions in China are asymmetric and can be characterized by contrasting features in both time and space in China. Spatially, most areas in western China have experienced transitions from drying to wetting, while opposite changes have occurred in most areas of eastern China. Temporally, the transitions occurred earlier in western China compared to eastern China. Research into the asymmetric spatial characteristics of dry/wet conditions compensates for the inadequacies of previous studies, which focused solely on temporal evolution;at the same time, it remedies the inadequacies of traditional research on linear trends over centennial timescales. Analyzing the non-linear trend also provides for a more comprehensive understanding of the drying/wetting changes in China.
基金the National Natural Science Foundation of China(42271289).
文摘Ecological stability is a core issue in ecological research and holds significant implications forhumanity. The increased frequency and intensity of drought and wet climate events resulting from climatechange pose a major threat to global ecological stability. Variations in stability among different ecosystemshave been confirmed, but it remains unclear whether there are differences in stability within the sameterrestrial vegetation ecosystem under the influence of climate events in different directions and intensities.China's grassland ecosystem includes most grassland types and is a good choice for studying this issue.This study used the Standardized Precipitation Evapotranspiration Index-12 (SPEI-12) to identify thedirections and intensities of different types of climate events, and based on Normalized DifferenceVegetation Index (NDVI), calculated the resistance and resilience of different grassland types for 30consecutive years from 1990 to 2019 (resistance and resilience are important indicators to measurestability). Based on a traditional regression model, standardized methods were integrated to analyze theimpacts of the intensity and duration of drought and wet events on vegetation stability. The resultsshowed that meadow steppe exhibited the highest stability, while alpine steppe and desert steppe had thelowest overall stability. The stability of typical steppe, alpine meadow, temperate meadow was at anintermediate level. Regarding the impact of the duration and intensity of climate events on vegetationecosystem stability for the same grassland type, the resilience of desert steppe during drought was mainlyaffected by the duration. In contrast, the impact of intensity was not significant. However, alpine steppewas mainly affected by intensity in wet environments, and duration had no significant impact. Ourconclusions can provide decision support for the future grassland ecosystem governance.
基金This work was supported by the National Natural Science Foundation of China(32071943,32272198).
文摘Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG.
基金supported by the Key Research and Development Program of Shaanxi,China(2018GY-067).
文摘In this study,the perovskite nanocomposite PrFe_(x)Co_(1-x)O_(3)(Pr(S))was successfully synthesized by the sol-gel method;PrFe_(x)Co_(1-x)O_(3)/Al-pillared montmorillonite(Pr(S)/Mt)catalysts were prepared by impregnation(D)method and solid-melting(G)method,respectively,with Pr(S)as the active component and Al-pillared montmorillonite as the carrier.The catalysts were applied to treat the 2-hydroxybenzoic acid(2-HA)-simulated wastewater by catalytic wet peroxide oxidation(CWPO)technique,and the chemical oxygen demand(COD)removal rate and the 2-HA degradation rate were used as indicators to evaluate the catalytic performance.The results of the experiment indicated that the solid-melting method was more conducive to preparing the catalyst when the Co/Fe molar ratio of 7:3 and the optimal structural properties of the catalysts were achieved.The influence of operating parameters,including reaction temperature,catalyst dosage,H_(2)O_(2)dosage,pH,and initial 2-HA concentration,were optimized for the degradation of 2-HA by CWPO.The results showed that 97.64%of 2-HA degradation and 75.23%of COD removal rate were achieved under more suitable experimental conditions.In addition,after the catalyst was used five times,the degradation rate of 2-HA could still reach 76.93%,which implied the high stability and reusability of the catalyst.The high catalytic activity of the catalyst was due to the doping of Co into PrFeO_(3),which could promote the generation of HO·,and the high stability could be attributed to the loading of Pr(S)onto Al-Mt,which reduced the leaching of reactive metals.The study of reaction mechanism and kinetics showed that the whole degradation process conformed to the pseudo-firstorder kinetic equation,and the Langmuir-Hinshelwood method was applied to demonstrate that catalysis was dominant in the degradation process.
基金The work described in this paper was partially supported by grants from the Research Grant Council of the Hong Kong Special Administrative Region,China(Project Nos.HKU 17207518 and R5037-18).
文摘The traditional standard wet sieving method uses steel sieves with aperture?0.063 mm and can only determine the particle size distribution(PSD)of gravel and sand in general soil.This paper extends the traditional method and presents an extended wet sieving method.The extended method uses both the steel sieves and the nylon filter cloth sieves.The apertures of the cloth sieves are smaller than 0.063 mm and equal 0.048 mm,0.038 mm,0.014 mm,0.012 mm,0.0063 mm,0.004 mm,0.003 mm,0.002 mm,and 0.001 mm,respectively.The extended method uses five steps to separate the general soil into many material sub-groups of gravel,sand,silt and clay with known particle size ranges.The complete PSD of the general soil is then calculated from the dry masses of the individual material sub-groups.The extended method is demonstrated with a general soil of completely decomposed granite(CDG)in Hong Kong,China.The silt and clay materials with different particle size ranges are further examined,checked and verified using stereomicroscopic observation,physical and chemical property tests.The results further confirm the correctness of the extended wet sieving method.
基金Supported by research grants from the National Key Research and Development Program of China(No.2020YFE0204400)the National Natural Science Foundation of China(No.82271042+1 种基金No.52203191)the Zhejiang Province Key Research and Development Program(No.2023C03090).
文摘●AIM:To determine the teaching effects of a real-time three dimensional(3D)visualization system in the operating room for early-stage phacoemulsification training.●METHODS:A total of 10 ophthalmology residents of the first-year postgraduate were included.All the residents were novices to cataract surgery.Real-time cataract surgical observations were performed using a custom-built 3D visualization system.The training lasted 4wk(32h)in all.A modified International Council of Ophthalmology’s Ophthalmology Surgical Competency Assessment Rubric(ICO-OSCAR)containing 4 specific steps of cataract surgery was applied.The self-assessment(self)and expert-assessment(expert)were performed through the microsurgical attempts in the wet lab for each participant.●RESULTS:Compared with pre-training assessments(self 3.2±0.8,expert 2.5±0.6),the overall mean scores of posttraining(self 5.2±0.4,expert 4.7±0.6)were significantly improved after real-time observation training of 3D visualization system(P<0.05).Scores of 4 surgical items were significantly improved both self and expert assessment after training(P<0.05).●CONCLUSION:The 3D observation training provides novice ophthalmic residents with a better understanding of intraocular microsurgical techniques.It is a useful tool to improve teaching efficiency of surgical education.
基金supported in part by the MOST Major Research and Development Project(Grant No.2021YFB2900204)the National Natural Science Foundation of China(NSFC)(Grant No.62201123,No.62132004,No.61971102)+3 种基金China Postdoctoral Science Foundation(Grant No.2022TQ0056)in part by the financial support of the Sichuan Science and Technology Program(Grant No.2022YFH0022)Sichuan Major R&D Project(Grant No.22QYCX0168)the Municipal Government of Quzhou(Grant No.2022D031)。
文摘Integrated data and energy transfer(IDET)enables the electromagnetic waves to transmit wireless energy at the same time of data delivery for lowpower devices.In this paper,an energy harvesting modulation(EHM)assisted multi-user IDET system is studied,where all the received signals at the users are exploited for energy harvesting without the degradation of wireless data transfer(WDT)performance.The joint IDET performance is then analysed theoretically by conceiving a practical time-dependent wireless channel.With the aid of the AO based algorithm,the average effective data rate among users are maximized by ensuring the BER and the wireless energy transfer(WET)performance.Simulation results validate and evaluate the IDET performance of the EHM assisted system,which also demonstrates that the optimal number of user clusters and IDET time slots should be allocated,in order to improve the WET and WDT performance.
文摘The main soil type, principle contributor of nutrients and available agricultural land in the Hula Valley is the organic Peat. Nevertheless, the relative contribution of Phosphorus from the Hula Valley to the Lake Kinneret inputs is lower than regional outsourcing. The Nitrogenic matter, mostly Nitrate migration from the Peat soil is significant. The implementation of efficient development is the key factor of Hula Land use. The financial beneficial success of the Hula land use is therefore dependent of Peat soil properties. The porosity of the Peat Soil is high and preferential pathway volume is low and Hydraulic Conductivity is therefore low. Consequently, the Mobile Spray Irrigation line was found as most suitable for cultivation in Peat Soil. Enhancement of Summer irrigation creating moisture elevation reduces Phosphorus migration from Peat Soil and is therefore recommended and recently implemented.
基金supported in part by ZTE Industry-University-Institute Co⁃operation Funds.
文摘Cell-free(CF)multiple-input multiple-output(MIMO)is a promising technique to enable the vision of ubiquitous wireless connectivity for next-generation network communications.Compared to traditional co-located massive MIMO,CF MIMO allows geographically distributed access points(APs)to serve all users on the same time-frequency resource with spatial multiplexing techniques,resulting in better performance in terms of both spectral efficiency and coverage enhancement.However,the performance gain is achieved at the expense of deploying more APs with high cost and power consumption.To address this issue,the recently proposed reconfigurable intelligent surface(RIS)technique stands out with its unique advantages of low cost,low energy consumption and programmability.In this paper,we provide an overview of RIS-assisted CF MIMO and its interaction with advanced optimization designs and novel applications.Particularly,recent studies on typical performance metrics such as energy efficiency(EE)and spectral efficiency(SE)are surveyed.Besides,the application of RIS-assisted CF MIMO techniques in various future communication systems is also envisioned.Additionally,we briefly discuss the technical challenges and open problems for this area to inspire research direction and fully exploit its potential in meeting the demands of future wireless communication systems.
文摘Introduction:Cupping therapy is an integrative and complementary medicine therapy that practiced worldwide by several civilizations for thousands of years.Cupping therapy is reported to be a relatively safe practice especially if performed by qualified therapists.Subconjunctival hemorrhage(SCH)is a common cause of eye redness and can be classified into traumatic and spontaneous.Case presentation:This case report discussed an adverse event related to wet cupping therapy.The patient developed SCH one day after performing cupping therapy.He was completely recovered after 2 weeks.This case report should increase the knowledge of cupping therapists regarding increasing safety of patient when performing wet cupping therapy on specific body parts especially,head,neck and cervical areas.Furthermore,it highlighted the importance of conducting research regarding the mechanical and physiological effects of cupping therapy.Conclusion:Adverse events related to cupping therapy should be reported with every patient.Cupping therapists should be aware of this possible mild adverse event.To the best of our knowledge,this is the first published adult case of SCH related to wet cupping therapy session.More studies are critically needed for evaluation of cupping therapy effects and adverse events especially when performed on head and neck.
基金Project supported by the Department of Science and Technology,Government of India(No.SR/FST/MS-I/2018/23(C))
文摘The present investigation focuses on the thermal performance of a fully wet stretching/shrinking longitudinal fin of exponential profile coated with a mechanism like a conveyer belt.The modeled equation is non-dimensionalized and solved by applying the Runge-Kutta-Fehlberg(RKF)method.The effects of parameters such as the wet parameter,the fin shape parameter,and the stretching/shrinking parameter on the heat transfer and thermal characteristics of the fin are graphically analyzed and discussed.It is inferred that the negative effects of motion and internal heat generation on the fin heat transfer rate can be lessened by setting a shrinking mechanism on the fin surface.The current examination is inclined towards practical applications and is beneficial to the design of fins.
文摘Extensive dust control on the dry Owens Lake mainly uses constructed basins that are flooded with shallow depths of fresh water. This dust control is mandated by law as a minimum percent of the area of each individual wetting basin. Wetted surfaces are evaluated for area and degree of wetness using the shortwave infrared (SWIR) band of Landsat TM, or similar earth observation satellite sensor. The SWIR region appropriate for these measurements lies within the electromagnetic spectrum between about 1.5 and 1.8 μm wavelengths. A threshold value for Landsat TM5 band 5 reflectance of 0.19 was found to conform with surfaces having a threshold for adequate wetting at a nascent point where rapid drying would occur following loss of capillary connection with groundwater. This threshold is robust and requires no atmospheric correction for the effects of aerosol scatter and attenuation as long as the features on the image appear clear. Monthly monitoring of surface wetting has proven accurate, verifiable and repeatable using these methods. This threshold can be calibrated for any Earth observation satellite that records the appropriate SWIR region. The monitoring program is expected to provide major input for the final phase of the dust control program that will have a focus to conserve water and resources.
基金the National Natural Science Foundation of China(52104155)Natural Science Foundation of Beijing(8212032)Fundamental Research Funds for the Central Universities(2023YQNY).
文摘Water–rock interaction(WRI)is a topic of interest in geology and geotechnical engineering.Many geological hazards and engineering safety problems are severe under the WRI.This study focuses on the water weakening of rock strength and its infuencing factors(water content,immersion time,and wetting–drying cycles).The strength of the rock mass decreases to varying degrees with water content,immersion time,and wetting–drying cycles depending on the rock mass type and mineral composition.The corresponding acoustic emission count and intensity and infrared radiation intensity also weaken accordingly.WRI enhances the plasticity of rock mass and reduces its brittleness.Various microscopic methods for studying the pore characterization and weakening mechanism of the WRI were compared and analyzed.Various methods should be adopted to study the pore evolution of WRI comprehensively.Microscopic methods are used to study the weakening mechanism of WRI.In future work,the mechanical parameters of rocks weakened under long-term water immersion(over years)should be considered,and more attention should be paid to how the laboratory scale is applied to the engineering scale.
基金supported by the UK–China Research & Innovation Partnership Fund through the Met Office Climate Science for Service Partnership (CSSP) China as part of the Newton Fund
文摘Heat events may be humid or dry.While several indices incorporate humidity,such combined indices obscure identification and exploration of heat events by their different humidity characteristics.The new HadISDH.extremes global gridded monitoring product uniquely provides a range of wet and dry bulb temperature extremes indices.Analysis of this new data product demonstrates its value as a tool for quantifying exposure to humid verses dry heat events.It also enables exploration into“stealth heat events”,where humidity is high,perhaps enough to affect productivity and health,while temperature remains moderate.Such events may not typically be identified as“heat events”by temperature-focused heat indices.Over 1973-2022,the peak magnitude of humid extremes(maximum daily wet bulb temperature over a month;T_(w)X)for the global annual mean increased significantly at 0.13±0.04℃(10 yr)^(−1),which is slightly slower than the global annual mean T_(w) increase of 0.22±0.04℃(10 yr)^(−1).The frequency of moderate humid extreme events per year(90th per-centile daily maxima wet bulb temperature exceedance;T_(w)X90p)also increased significantly at 4.61±1.07 d yr^(−1)(10 yr)^(−1).These rates were slower than for temperature extremes,TX and TX90p,which respectively increased significantly at 0.27±0.04℃(10 yr)^(−1) and 5.53±0.72 d yr^(−1)(10 yr)^(−1).Similarly,for the UK/Europe focus region,JJA-mean T_(w)X increased significantly,again at a slower rate than for TX and mean T_(w).HadISDH.extremes shows some evidence of“stealth heat events”occurring where humidity is high but temperature remains more moderate.
基金supported by the UK–China Research & Innovation Partnership Fund through the Met Office Climate Science for Service Partnership (CSSP) China as part of the Newton Fund
文摘HadISDH.extremes is an annually updated global gridded monthly monitoring product of wet and dry bulb temperature–based extremes indices,from January 1973 to December 2022.Data quality,including spatial and temporal stability,is a key focus.The hourly data are quality controlled.Homogeneity is assessed on monthly means and used to score each gridbox according to its homogeneity rather than to apply adjustments.This enables user-specific screening for temporal stability and avoids errors from inferring adjustments from monthly means for the daily maximum values.For general use,a score(HQ Flag)of 0 to 6 is recommended.A range of indices are presented,aligning with existing standardised indices.Uniquely,provision of both wet and dry bulb indices allows exploration of heat event character—whether it is a“humid and hot”,“dry and hot”or“humid and warm”event.It is designed for analysis of long-term trends in regional features.HadISDH.extremes can be used to study local events,but given the greater vulnerability to errors of maximum compared to mean values,cross-validation with independent information is advised.
文摘The wide engineered application of compacted expansive soils necessitates understanding their behavior under field conditions.The results of this study demonstrate how seasonal climatic variation and stress and boundary conditions individually or collectively influence the hydraulic and volume change behavior of compacted highly expansive soils.The cyclic wetting and drying(CWD)process was applied for two boundary conditions,i.e.constant stress(CS)and constant volume(CV),and for a wide range of axial stress states.The adopted CWD process affected the hydraulic and volume change behaviors of expansive soils,with the first cycle of wetting and drying being the most effective.The CWD process under CS conditions resulted in shrinkage accumulation and reduction in saturated hydraulic conductivity(k sat).On the other hand,CWD under CV conditions caused a reduction of swell pressure while has almost no impact on k sat.An elastic response to CWD was achieved after the third cycle for saturated hydraulic conductivity(k sat),the third to fourth cycle for the volume change potential under the CV conditions,and the fourth to fifth cycle for the volume change potential under the CS conditions.Finally,both swell pressure(s s)and saturated hydraulic conductivity(k sat)are not fundamental parameters of the expansive soil but rather depend on stress,boundary and wetting conditions.
基金the Geological Survey Project of China(Hydrogeology and Water Resources Survey and Monitoring in Hexi Corridor,China.No.DD20221752-2).
文摘The objective of this study was to analyze the response of runoff in the area of runoff yield of the upstream Shiyang River basin to climate change and to promote sustainable development of regional water resources and ecological environment.As the biggest tributary of the Shiyang River,Xiying River is the only hydrological station(Jiutiaoling) that has provincial natural river and can achieve long time series monitoring data in the basin.The data obtained from this station is representative of natural conditions because it has little human activites.This study built a regression model through identifying the characteristics of runoff and climate change by using Mann-Kendall nonparametric statistical test,cumulative anomaly,and correlation analysis.The results show that the average annual runoff is 320.6 million m3/a with the coefficient of variation of 0.18 and shows slightly decrease during 1956-2020.It has a significant positive correlation the average annual precipitation(P<0.01).Runoff is sensitive to climate change,and the climate has becoming warm and wet and annual runoff has entering wet period from 2003.Compared to the earlier period(1955-2000),the increases of average annual temperature,precipitation and runoff in recent two decades were 15%,9.3%,and 7.8%,respectively.Runoff in the Shiyang River is affected by temperature and precipitation among climate factors,and the simulation results of the runoff-climate response model(R=0.0052P-0.1589T+2.373) indicate that higher temperature leads to a weakening of the ecological regulation of surface runoff in the flow-producing area.
基金the National Natural Science Foundation of China(51904329,52174014)the Major Scientific and Technological Projects of CNPC(ZD 2019-183-005)Key R&D Program of Shandong Province(No.2020ZLYS07).
文摘Wellbore instability,especially drilling with water-based drilling fluids(WBDFs)in complex shale for-mations,is a critical challenge for oil and gas development.The purpose of this paper is to study the feasibility of using hydrophobically modified silica nanoparticle(HMN)to enhance the comprehensive performance of WBDFs in the Xinjiang Oilfield,especially the anti-collapse performance.The effect of HMN on the overall performance of WBDFs in the Xinjiang Oilfield,including inhibition,plugging,lu-bricity,rheology,and filtration loss,was studied with a series of experiments.The mechanism of HMN action was studied by analyzing the changes of shale surface structure and chemical groups,wettability,and capillary force.The experimental results showed that HMN could improve the performance of WBDFs in the Xinjiang Oilfeld to inhibit the hydration swelling and dispersion of shale.The plugging and lubrication performance of the WBDFs in the Xinjiang Oilfield were also enhanced with HMN based on the experimental results.HMN had less impact on the rheological and filtration performance of the WBDFs in the Xinjiang Oilfield.In addition,HMN significantly prevented the decrease of shale strength.The potential mechanism of HMN was as follows.The chemical composition and structure of the shale surface were altered due to the adsorption of HMN driven by electrostatic attraction.Changes of the shale surface resulted in significant wettability transition.The capillary force of the shale was converted from a driving force of water into the interior to a resistance.In summary,hydrophobic nanoparticles presented afavorable application potential for WBDFs.
基金supported by National Natural Science Foundation of China(Grant No.11902029)。
文摘Inflatable wing has significant application value in the design of loitering munitions because of its advantages such as lightweight and foldability.However,due to the flexible characteristics,aeroelastic behaviors of inflatable wings such as flutter are nonnegligible in flight.By designing a certain angle between the inflatable beam and the wing span,the structural dynamic and even the aeroelastic performance of the inflatable wing can be effectively improved.Based on the analysis of the mechanical and geometric characteristics of the inflatable structure,a new inflatable wing with sweep arranged inflatable beams is proposed,and the main design variables and methods are analyzed.For purpose of investigating the aeroelastic performance of the swept baffled inflatable wing,the modal behaviors by considering the wet mode are studied.In consideration of the deficiencies of the traditional wet modal analysis method,by introducing the influence on the additional stiffness of flow field,an added massstiffness method is proposed in this paper,and the advantages are verified by ground vibration experiments.On this basis,the effects of baffles sweep angle,pressure,and boundary conditions on the modal parameters and aeroelastic performance of inflatable wing are analyzed.The results show that the aeroelastic performance of the inflatable wing can be designed by changing the baffles sweep angle,which is enlightened for the aeroelastic tailoring design on inflatable wings.
基金AGER-Fondazioni in rete per la ricerca agroalimentare(https://www.progettoager.it/)(Grant No.2010-2369)by Joint Programming Initiative on Agriculture,Food Security and Climate Change(FACCE-JPI)project Green Rice(Sustainable and environmental friendly rice cultivation systems in Europe).
文摘Alternate wetting and drying(AWD)system,in which water has been reduced by approximately 35%with an increased occurrence of beneficial arbuscular mycorrhizal(AM)symbiosis and no negative impact on rice yield,was proposed to utilize water and nutrients more sustainable.In this study,we selected six rice cultivars(Centauro,Loto,Selenio,Vialone nano,JSendra and Puntal)grown under AWD conditions,and investigated their responsiveness to AM colonization and how they select diverse AM taxa.In order to investigate root-associated AM fungus communities,molecular cloning-Sanger sequencing on small subunit rDNA data were obtained from five out of the six rice cultivars and compared with Next Generation Sequencing(NGS)data,which were previously obtained in Vialone nano.The results showed that all the cultivars were responsive to AM colonization with the development of AM symbiotic structures,even if with differences in the colonization and arbuscule abundance in the root systems.We identified 16 virtual taxa(VT)in the soil compartment and 7 VT in the root apparatus.We emphasized that the NGS analysis gives additional value to the results thanks to a more in-depth reading of the less represented AM fungus taxa.