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A Simple and Quick Method of Extracting Genomic DNA from Wheat Leaves 被引量:12
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作者 王敏 那冬晨 +1 位作者 姬虎太 张定一 《Agricultural Science & Technology》 CAS 2009年第5期34-35,75,共3页
[ Objective] The aim was to seek a simple and quick method of extracting genomic DNA from wheat leaves. [ Method] Taking tender leaves of wheat as test materials, total DNA of transgenic wheat was extracted by using m... [ Objective] The aim was to seek a simple and quick method of extracting genomic DNA from wheat leaves. [ Method] Taking tender leaves of wheat as test materials, total DNA of transgenic wheat was extracted by using modified CTAB method. The extracted DNA was detected by 0.8% agarose gel electrophoresis. [ Result] DNA purity of extracted genome DNA from wheat was high and no degradation phenomenon using modified CTAB method, and was suitable for carrying out normal PCR amplification. [ Conclusion] This study provides a simple and quick method for extracting DNA from wheat with a spot of material. 展开更多
关键词 wheat DNA extraction Simple and quick
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Antitumor Activities and Apoptosis-regulated Mechanisms of Fermented Wheat Germ Extract in the Transplantation Tumor Model of Human HT-29 Cells in Nude Mice 被引量:4
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作者 ZHANG Jia Yan XIAO Xiang +2 位作者 DONG Ying WU Jing ZHOU Xing Hua 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2015年第10期718-727,共10页
Objective A subcutaneous transplantation tumor model of human HT-29 cells in nude mice was established to evaluate anticarcinogenic activities, and the apoptosis-regulated mechanism effect of aqueous extract of fermen... Objective A subcutaneous transplantation tumor model of human HT-29 cells in nude mice was established to evaluate anticarcinogenic activities, and the apoptosis-regulated mechanism effect of aqueous extract of fermented wheat germ with Lactobacillus plantarum dy-1 (LFWGE). Methods The HT-29 cells were transplanted via subcutaneous injection of 1×10^7cells into the right flank of each nude mouse. Then, nude mice were treated for 30 d with LFWGE (high-dose 2 g/kg/d; low-dose 1 g/kg/d) and for 7 d with 5-fluorouracil (5-FU, 25 mg/kg/d) by gavage and intraperitoneal injection, respectively. An inhibition of tumor growth was observed. Results Tumor volume and weights decreased significantly in both groups of nude mice treated with LFWGE. In addition, the cell apoptosis rate of the LFWGE group (2 g/kg/d, 60.2%+4.4%; 1 g/kg/d, 58.6%+6.9%) was significantly higher than that of the control group (11.5%+1.6%) and 5-FU group (32.1%+3.5%) as measured by the TUNEL assay. Moreover, the real-time fluorescent quantitative PCR and Western blot method further confirmed these enhancing apoptosis and growth inhibition effects. The involvement of LFWGE in inducing apoptosis was confirmed by the expression of Bax, Bcl-2, Caspase-3, and CyclinD1. Conclusion The results showed that LFWGE could induce subcutaneous transplantation tumor apoptosis in nude mice and could be as a natural nutrient supplements or chemopreventive agent in the treatment of human colon cancer. 展开更多
关键词 Fermented wheat germ extract Nude mice ANTITUMOR APOPTOSIS Western blot Human HT-29 cells
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Determination of the Anti-Adhesive and Anti-Biofilm Capacity of a Wheat Extract on <i>Staphylococcus aureus</i>in Farms
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作者 Maria Angeles Calvo Torras Esteban Leonardo Arosemena Angulo +1 位作者 Susana Martin Orúe Xavier Torras Bombardó 《Journal of Materials Science and Chemical Engineering》 2021年第9期11-21,共11页
The formation of biofilm by pathogenic microorganisms has become a problem in the livestock industry since it is considered a potential source of infection for farm animals while increasing microbial resistance to phy... The formation of biofilm by pathogenic microorganisms has become a problem in the livestock industry since it is considered a potential source of infection for farm animals while increasing microbial resistance to physical and chemical agents. Some plant extracts, such as soluble wheat extract, have been shown to be effective in inhibiting or destroying the biofilm of certain micro-organisms under specific conditions. The objective of this study is to evaluate the capacity of the pathogen to form biofilm on different surfaces used in livestock, as well as to evaluate the anti-biofilm capacity of the soluble wheat extract against <i>S. aureus</i> on these surfaces. The inhibition potential of inhibition or destruction of biofilm was tested in vitro. Wheat extract at a concentration of 0.29 mg/100mL showed anti-biofilm activity on <i>S. aureus</i>, inhibiting its formation, as well as destroying it greatly after a contact time of 24 hours, on those surfaces where the microorganism presents more adhesion capacity. 展开更多
关键词 Microbiology Biofilm Anti-Biofilm wheat Extract Staphylococcus Aureus
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