On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cult...On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.展开更多
To maintain high wheat grain yield in areas where frequent and periodic waterlogging occurs,the effects of waterlogging on the photosynthesis,growth,yield,and protein content of three wheat cultivars,namely Xiangmai55...To maintain high wheat grain yield in areas where frequent and periodic waterlogging occurs,the effects of waterlogging on the photosynthesis,growth,yield,and protein content of three wheat cultivars,namely Xiangmai55(X55),Jingmai102(J102),and Zhengmai9023(Z9023),in four different growth stages were investigated.Experiments were conducted in specially designed experimental tanks in the middle and lower reaches of the Yangtze River during the 2012-2013 and 2013-2014 wheat growing seasons in China.Results showed that X55 was the most susceptible to waterlogging,followed by J102 and Z9023.Chlorophyll content reduction and leaf senescence,which resulted in decreased green-to-total leaf number ratio,were induced to the greatest extents by waterlogging in booting and flowering stages,followed by milky stage.Meanwhile,chlorophyll content in flag leaf,plant height were significantly decreased by waterlogging in jointing stage but effectively recovered after waterlogging withdrawal,and recovery ability varied among the cultivars.Plant biomass and grain yield were most significantly decreased by waterlogging in booting and flowering stages,followed by milky and jointing stages.Grain protein content was also considerably affected by waterlogging depending on growth stage and cultivars.The decreased grain yield caused by waterlogging was mostly due to the sharp decline in 1 000-grain mass.Waterlogging led to reduced protein yield in all growth stages in three wheat cultivars.Above all,in this experiment,waterlogging decreased grain yield significantly,and waterlogging at booting stage and flowering stage was most serious.Comparing the three cultivars,X55 was most sensitive to waterlogging.展开更多
A crop growth model,integrating genotype,environment,and management factor,was developed to serve as an analytical tool to study the influence of these factors on crop growth,production,and agricultural planning.A maj...A crop growth model,integrating genotype,environment,and management factor,was developed to serve as an analytical tool to study the influence of these factors on crop growth,production,and agricultural planning.A major challenge of model application is the optimization and calibration of a considerable number of parameters.Sensitivity analysis(SA) has become an effective method to identify the importance of various parameters.In this study,the extended Fourier Amplitude Sensitivity Test(EFAST) approach was used to evaluate the sensitivity of the DSSAT-CERES model output responses of interest to 39 crop genotype parameters and six soil parameters.The outputs for the SA included grain yield and quality(take grain protein content(GPC) as an indicator) at maturity stage,as well as leaf area index,aboveground biomass,and aboveground nitrogen accumulation at the critical process variables.The key results showed that:(1) the influence of parameter bounds on the sensitivity results was slight and less than the impacts from the significance of the parameters themselves;(2) the sensitivity parameters of grain yield and GPC were different,and the sensitivity of the interactions between parameters to GPC was greater than those between the parameters to grain yield;and(3) the sensitivity analyses of some process variables,including leaf area index,aboveground biomass,and aboveground nitrogen accumulation,should be performed differently.Finally,some parameters,which improve the model’s structure and the accuracy of the process simulation,should not be ignored when maturity output as an objective variable is studied.展开更多
The NAM-B1 gene is a member of the NAC（NAM,ATAF,and CUC）transcription factor family and plays an important role in regulating wheat grain protein content（GPC）.The ancestral NAM-B1 allele has been discovered in man...The NAM-B1 gene is a member of the NAC（NAM,ATAF,and CUC）transcription factor family and plays an important role in regulating wheat grain protein content（GPC）.The ancestral NAM-B1 allele has been discovered in many tetraploid wild emmer（Triticum turgidum ssp.dicoccoides）accessions and few domesticated emmer accessions（T.turgidum ssp.dicoccum）,however,it is rarely found in hexaploid bread wheat（Triticum aestivum L.）.There are no systematic reports on the distribution of NAM-B1 alleles in Chinese wheat cultivars.In this study,the NAM-B1 alleles in 218 Chinese cultivars were investigated.The cultivars were collected from five major wheat regions（12 provinces）,covering most of the winter wheat growing regions in China.The results showed that the NAM-B1 gene is present in 53（24.3%）cultivars and absent in the remaining 165（75.7%）cultivars.Further analysis revealed that in contrast to the wild-type allele,the NAM-B1 gene in Chinese wheat cultivars contained a 1-bp insertion in the coding region.This caused a frame-shift mutation and introduced a stop codon in the middle of the gene,rendering it non-functional.Polymorphisms were detected in DNA sequences of 21cultivars among these 53 cultivars.However,cD NA sequence analysis suggested that these variations in the exon region were not able to restore NAM-B1 gene（1-bp insertion）function.Thus,exploring the distribution of NAM-B1 gene variations（1-bp insertion and deletion）can provide some information for improving the quality of winter wheat in China and other countries.展开更多
Wheat is a staple crop worldwide, but yields may diminish as climate change causes increasingly unpredictable patterns of precipitation and soil nutrient availability. Farmers are thus challenged to maximize planting ...Wheat is a staple crop worldwide, but yields may diminish as climate change causes increasingly unpredictable patterns of precipitation and soil nutrient availability. Farmers are thus challenged to maximize planting efficiency to increase yield, while also improving their resource use efficiency. In this study the effectiveness of tridimensional uniform sowing was tested across a range of planting densities for winter wheat crops on the North China Plain. Tridimensional uniform sowing was tested against conventional drilling at three planting densities (180 × 104, 270 × 104, and 360 × 104 plants ha 1) and assessed for water consumption, biomass, nitrogen uptake and allocation, and aspects of yield. The tridimensional uniform sowing treatment outperformed the conventional drilling treatment in most metrics and at most planting densities, while performing markedly better at higher planting densities. Water consumption decreased and nitrogen efficiency increased. Tiller number and percentage of productive tillers, leaf area index, dry weight, and yield increased without a significant decline in grain protein. Nitrogen allocation was more efficient under tridimensional uniform sowing than with conventional drilling, and also varied according to annual precipitation and planting density. Both yield and grain protein contents were significantly correlated with the amount of pre-anthesis accumu- lated nitrogen translocated from vegetative organs to kernels after anthesis. Overall, a density of 270 × 104 plants ha 1 provided the highest water use efficiency and grain yield. Tridimensional uniform sowing will benefit farmers by forming stronger overall crops, promoting the coordinated improvement of yield, nitrogen uptake and efficiency, and increasing grain protein content at higher planting densities.展开更多
Mechanically post-harvest puddled rice field has stubbles that often delay timely planting of winter wheat crop. Zero tillage increased the net return by decreasing the unwise tillage operations and labor charges. Kee...Mechanically post-harvest puddled rice field has stubbles that often delay timely planting of winter wheat crop. Zero tillage increased the net return by decreasing the unwise tillage operations and labor charges. Keep in view, a randomized complete block design experiment in a split plot arrangement was conducted with four tillage system [conventional tillage, CT;deep tillage, DT;zero tillage with zone disc tiller, ZDT;and happy seeder, HS] in main plots and five nitrogen levels [0, 75, 100, 125, and 150 kg·ha-1] in subplots during 2009 to 2010 and 2010 to 2011 cropping seasons. Results showed that in 2009-10 and 2010-11 grain yield (4.6 Mg·ha-1 and 5.7 Mg·ha-1) in DT and (4.5 Mg·ha-1 and 5.8 Mg·ha-1) in HS were significantly higher compared with CT and ZDT. Significantly, maximum leaf area index (5.18 and 5.24) and crop growth rate (12.14 g·m-2·d-1 and 13.15 g·m-2·d-1) were noted in DT. Grain protein (11.78%) was significantly higher in DT compared with CT, ZDT, and HS during 2009-10 and 2010-11. Total yield (12.4 Mg·ha-1 and 16.4 Mg·ha-1) and grain yield (4.9 Mg·ha-1 and 6.5 Mg·ha-1) at N125 kg·ha-1 while grain protein (13.52%) at N150 kg·ha-1 was significantly higher than other nitrogen levels. Maximum LAI (5.08 and 5.51) and crop growth rate (14.68 g m-2·d-1 and 15.77 g<span style='font-family:Verdana, Helvetica, Arial;white-space:normal;展开更多
文摘On the basis of previous studies dealing with the variation of major agronomic and yield characteristics of regenerated plants derived from single cell culture in vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and its fractions from regenerated plants with stable agronomic characteristics were studied from 1992 to 1995. The results showed that the variation of grain protein content and its fractions in somaclones from single cell culture in vitro were very significant and the range was very wide (11531770%). Several types of variation were found in the studies, especially the type with higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of lines the grain protein content was significantly higher than that of NE 7742 and combined with high yielding potential. The tendency of variation of the four protein fractions showed that the variation of albumin was not obvious and maintained the same level as NE774 increased in some somaclones and decreased in others. However, the percentages both globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and glutenin tended to increase. The variation of total amount of structural protein and the ratio between globulin and albumm was mainly influenced by globulin under the condition of culture in vitro. The variation of total amount of storage protein and the ratio between gliadin and glutenin was mainly affected by glutenin. The results mentioned above demonstrated that the induction and screening of somaclonal variation could be an effective way in wheat improvement in combining high protein content with high yield.
基金Supported by the National Science Foundation of China(31371580)
文摘To maintain high wheat grain yield in areas where frequent and periodic waterlogging occurs,the effects of waterlogging on the photosynthesis,growth,yield,and protein content of three wheat cultivars,namely Xiangmai55(X55),Jingmai102(J102),and Zhengmai9023(Z9023),in four different growth stages were investigated.Experiments were conducted in specially designed experimental tanks in the middle and lower reaches of the Yangtze River during the 2012-2013 and 2013-2014 wheat growing seasons in China.Results showed that X55 was the most susceptible to waterlogging,followed by J102 and Z9023.Chlorophyll content reduction and leaf senescence,which resulted in decreased green-to-total leaf number ratio,were induced to the greatest extents by waterlogging in booting and flowering stages,followed by milky stage.Meanwhile,chlorophyll content in flag leaf,plant height were significantly decreased by waterlogging in jointing stage but effectively recovered after waterlogging withdrawal,and recovery ability varied among the cultivars.Plant biomass and grain yield were most significantly decreased by waterlogging in booting and flowering stages,followed by milky and jointing stages.Grain protein content was also considerably affected by waterlogging depending on growth stage and cultivars.The decreased grain yield caused by waterlogging was mostly due to the sharp decline in 1 000-grain mass.Waterlogging led to reduced protein yield in all growth stages in three wheat cultivars.Above all,in this experiment,waterlogging decreased grain yield significantly,and waterlogging at booting stage and flowering stage was most serious.Comparing the three cultivars,X55 was most sensitive to waterlogging.
基金supported by the National Natural Science Foundation of China(41701375,41601369,and 41471285)the European Space Agency(ESA)and Ministry of Science and Technology of China(MOST)Dragon 4 Cooperation Programme(32275-1)
文摘A crop growth model,integrating genotype,environment,and management factor,was developed to serve as an analytical tool to study the influence of these factors on crop growth,production,and agricultural planning.A major challenge of model application is the optimization and calibration of a considerable number of parameters.Sensitivity analysis(SA) has become an effective method to identify the importance of various parameters.In this study,the extended Fourier Amplitude Sensitivity Test(EFAST) approach was used to evaluate the sensitivity of the DSSAT-CERES model output responses of interest to 39 crop genotype parameters and six soil parameters.The outputs for the SA included grain yield and quality(take grain protein content(GPC) as an indicator) at maturity stage,as well as leaf area index,aboveground biomass,and aboveground nitrogen accumulation at the critical process variables.The key results showed that:(1) the influence of parameter bounds on the sensitivity results was slight and less than the impacts from the significance of the parameters themselves;(2) the sensitivity parameters of grain yield and GPC were different,and the sensitivity of the interactions between parameters to GPC was greater than those between the parameters to grain yield;and(3) the sensitivity analyses of some process variables,including leaf area index,aboveground biomass,and aboveground nitrogen accumulation,should be performed differently.Finally,some parameters,which improve the model’s structure and the accuracy of the process simulation,should not be ignored when maturity output as an objective variable is studied.
基金supported by the National Natural Science Founding of China (31401378)the Major Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences, China (2014CXZ10)+2 种基金the Youth Foundation of Shandong Academy of Agricultural Sciences, China (2014QNZ02)the Program for Youth Talent of Shandong Academy of Agricultural Sciences, China (118005)the Science & Technology Development Plan of Shandong Province, China (2014GSF121001)
文摘The NAM-B1 gene is a member of the NAC（NAM,ATAF,and CUC）transcription factor family and plays an important role in regulating wheat grain protein content（GPC）.The ancestral NAM-B1 allele has been discovered in many tetraploid wild emmer（Triticum turgidum ssp.dicoccoides）accessions and few domesticated emmer accessions（T.turgidum ssp.dicoccum）,however,it is rarely found in hexaploid bread wheat（Triticum aestivum L.）.There are no systematic reports on the distribution of NAM-B1 alleles in Chinese wheat cultivars.In this study,the NAM-B1 alleles in 218 Chinese cultivars were investigated.The cultivars were collected from five major wheat regions（12 provinces）,covering most of the winter wheat growing regions in China.The results showed that the NAM-B1 gene is present in 53（24.3%）cultivars and absent in the remaining 165（75.7%）cultivars.Further analysis revealed that in contrast to the wild-type allele,the NAM-B1 gene in Chinese wheat cultivars contained a 1-bp insertion in the coding region.This caused a frame-shift mutation and introduced a stop codon in the middle of the gene,rendering it non-functional.Polymorphisms were detected in DNA sequences of 21cultivars among these 53 cultivars.However,cD NA sequence analysis suggested that these variations in the exon region were not able to restore NAM-B1 gene（1-bp insertion）function.Thus,exploring the distribution of NAM-B1 gene variations（1-bp insertion and deletion）can provide some information for improving the quality of winter wheat in China and other countries.
基金supported by the National Key Research and Development Program of China (2016YFD0300407)Earmarked Fund for China Agriculture Research System (CARS-03)Agricultural Technology Test Demonstration and Service Support (118003)
文摘Wheat is a staple crop worldwide, but yields may diminish as climate change causes increasingly unpredictable patterns of precipitation and soil nutrient availability. Farmers are thus challenged to maximize planting efficiency to increase yield, while also improving their resource use efficiency. In this study the effectiveness of tridimensional uniform sowing was tested across a range of planting densities for winter wheat crops on the North China Plain. Tridimensional uniform sowing was tested against conventional drilling at three planting densities (180 × 104, 270 × 104, and 360 × 104 plants ha 1) and assessed for water consumption, biomass, nitrogen uptake and allocation, and aspects of yield. The tridimensional uniform sowing treatment outperformed the conventional drilling treatment in most metrics and at most planting densities, while performing markedly better at higher planting densities. Water consumption decreased and nitrogen efficiency increased. Tiller number and percentage of productive tillers, leaf area index, dry weight, and yield increased without a significant decline in grain protein. Nitrogen allocation was more efficient under tridimensional uniform sowing than with conventional drilling, and also varied according to annual precipitation and planting density. Both yield and grain protein contents were significantly correlated with the amount of pre-anthesis accumu- lated nitrogen translocated from vegetative organs to kernels after anthesis. Overall, a density of 270 × 104 plants ha 1 provided the highest water use efficiency and grain yield. Tridimensional uniform sowing will benefit farmers by forming stronger overall crops, promoting the coordinated improvement of yield, nitrogen uptake and efficiency, and increasing grain protein content at higher planting densities.
文摘Mechanically post-harvest puddled rice field has stubbles that often delay timely planting of winter wheat crop. Zero tillage increased the net return by decreasing the unwise tillage operations and labor charges. Keep in view, a randomized complete block design experiment in a split plot arrangement was conducted with four tillage system [conventional tillage, CT;deep tillage, DT;zero tillage with zone disc tiller, ZDT;and happy seeder, HS] in main plots and five nitrogen levels [0, 75, 100, 125, and 150 kg·ha-1] in subplots during 2009 to 2010 and 2010 to 2011 cropping seasons. Results showed that in 2009-10 and 2010-11 grain yield (4.6 Mg·ha-1 and 5.7 Mg·ha-1) in DT and (4.5 Mg·ha-1 and 5.8 Mg·ha-1) in HS were significantly higher compared with CT and ZDT. Significantly, maximum leaf area index (5.18 and 5.24) and crop growth rate (12.14 g·m-2·d-1 and 13.15 g·m-2·d-1) were noted in DT. Grain protein (11.78%) was significantly higher in DT compared with CT, ZDT, and HS during 2009-10 and 2010-11. Total yield (12.4 Mg·ha-1 and 16.4 Mg·ha-1) and grain yield (4.9 Mg·ha-1 and 6.5 Mg·ha-1) at N125 kg·ha-1 while grain protein (13.52%) at N150 kg·ha-1 was significantly higher than other nitrogen levels. Maximum LAI (5.08 and 5.51) and crop growth rate (14.68 g m-2·d-1 and 15.77 g<span style='font-family:Verdana, Helvetica, Arial;white-space:normal;
