Delayed sowing mitigates lodging in wheat. However, the mechanism underlying the enhanced lodging resistance in wheat has yet to be fully elucidated. Field experiments were conducted to investigate the effects of sowi...Delayed sowing mitigates lodging in wheat. However, the mechanism underlying the enhanced lodging resistance in wheat has yet to be fully elucidated. Field experiments were conducted to investigate the effects of sowing date on lignin and cellulose metabolism, stem morphological characteristics, lodging resistance, and grain yield. Seeds of Tainong 18,a winter wheat variety, were sown on October 8(normal sowing) and October 22(late sowing) during both of the 2015–2016 and 2016–2017 growing seasons. The results showed that late sowing enhanced the lodging resistance of wheat by improving the biosynthesis and accumulation of lignin and cellulose. Under late sowing, the expression levels of key genes(Ta PAL, Ta CCR, Ta COMT, TaCAD, and TaCesA1, 3, 4, 7, and 8) and enzyme activities(TaPAL and TaCAD) related to lignin and cellulose biosynthesis peaked 4–12 days earlier, and except for the TaPAL, TaCCR, and TaCesA1 genes and TaPAL, in most cases they were significantly higher than under normal sowing. As a result, lignin and cellulose accumulated quickly during the stem elongation stage. The mean and maximum accumulation rates of lignin and cellulose increased, the maximum accumulation contents of lignin and cellulose were higher, and the cellulose accumulation duration was prolonged. Consequently, the lignin/cellulose ratio and lignin content were increased from 0 day and the cellulose content was increased from 11 days after jointing onward. Our main finding is that the improved biosynthesis and accumulation of lignin and cellulose were responsible for increasing the stem-filling degree, breaking strength, and lodging resistance. The major functional genes enhancing lodging resistance in wheat that are induced by delayed sowing need to be determined.展开更多
Ammonia volatilized from aboveground parts of winter wheat was collected with an enclosure growth chamber and measured from jointing to maturing stage. The results showed that ammonia released from unfertilized plants...Ammonia volatilized from aboveground parts of winter wheat was collected with an enclosure growth chamber and measured from jointing to maturing stage. The results showed that ammonia released from unfertilized plants grown in high and low fertility soils remained at low rates of 2.3 and 0. 9μg NH3 40 plant-1 h-1 respectively at late filling stage. However, fertilized plants rapidly increased the rates to 43. 4 and 52. 2μg NH3, 40 plant-1 h-1 in the high and low fertility soils, respectively, at the same period. The released a-mount was different in different parts of plants. At filling stage, lower senescing stems and leaves volatilized more ammonia than upper parts, i. e. , ears and flag leaves that grew normally, with an average of 1. 4 and 0.7μg NH3 20 plant-1 h-1 respectively, strongly suggesting that it was the senile organs that released large amounts of ammonia. At the grain filling stage, shortage of water supply (drought stress) reduced ammonia volatilization. The average rate of ammonia released under water stress was 0. 9μg NH3 40 plant-1 h-1, but 1.2μg NH3 40 plant-1 h-1 with moderate water supply. Application of N together with P fertilizer resulted in a higher ammonia volatilization than N fertilization alone at the maturing stage. The average rate released was 135.3 μg NH3 40 plant-1 h-1 when 0.4 g N and 0.13 g P had been added to per kg soil, while 33. 7μg when 0. 4 g N added alone. Ammonia volatilization from plants was closely related with plant biomass and N uptake;P fertilization increased plant biomass and N uptake and therefore increased its release.展开更多
Late spring cold(LSC) occurred in the reproductive period of wheat impairs spike and floret differentiation during the reproductive period,when young spikelets are very cold-sensitive.However,under LSC,the responses o...Late spring cold(LSC) occurred in the reproductive period of wheat impairs spike and floret differentiation during the reproductive period,when young spikelets are very cold-sensitive.However,under LSC,the responses of wheat spikelets at various positions,leaves,and stems and the interactions between them at physiological levels remain unclear.In the present study,two-year treatments at terminal spikelet stage under two temperatures(2 C,-2 C) and durations(1,2,and 3 days) were imposed in an artificial climate chamber to compare the effects of LSC on grain number and yield in the wheat cultivars Yannong 19(YN19,cold-tolerant) and Xinmai 26(XM26,cold-sensitive).The night temperature regimes were designed to reproduce natural temperature variation.LSC delayed plant growth and inhibited spike and floret differentiation,leading to high yield losses in both cultivars.LSC reduced dry matter accumulation(DMA,g) in spikes,stems,and leaves,reducing the DMA ratios of the spike to leaf and spike to stem.Plant cell wall invertase(CWINV) activity increased in upper and basal spikelets in YN19,whereas CWINV increased in middle spikelets in XM26.Under LSC,soluble sugar and glucose were transported and distributed mainly in upper and basal spikelets for glume and rachis development,so that spike development was relatively complete in YN19,whereas the upper and basal spikelets were severely damaged and most of the glumes in middle spikelets were relatively completely developed in XM26,resulting in pollen abortion mainly in upper and basal spikelets.The development of glumes and rachides was influenced and grain number per spike was decreased after LSC,with kernels present mainly in middle spikelets.Overall,reduced total DMA and dry matter partitioning to spikes under LSC results in poor spikelet development,leading to high losses of grain yield.展开更多
[Objective] The research aimed to study influence of meteorological factor in late growth stage of wheat. [Method] Based on precipitation, sunshine and yield per unit of wheat in Anyang City in May of 1979-2008, the p...[Objective] The research aimed to study influence of meteorological factor in late growth stage of wheat. [Method] Based on precipitation, sunshine and yield per unit of wheat in Anyang City in May of 1979-2008, the positive and negative influences of meteorological condition in late growth stage of wheat (May) on wheat yield in Anyang City were analyzed by using agricultural climatic statistical method. Moreover, the reason and defense measure of green-dry hazard in late growth stage of wheat in the city were studied. [Result] When the sunshine percentage in May > 55%, and rainfall < 45 mm, the wheat yield generally increased. But when it was overcast and rainy, and the sunshine was less, especially monthly rainfall > 80 mm, and monthly sunshine percentage < 55%, the wheat yield generally reduced. The overcast and rainy weather, flood in late growth stage of wheat were easy to cause green-dry yield reduction. The rainless weather even drought weren’t obviously unfavorable for good harvest of wheat. In May, when precipitation was too more, or duration was too long, and the air humidity was too big, the normal water supply and inorganic nutrient transmission were affected. Meanwhile, when the overcast and rainy weather was longer, the sunshine was shorter, and the sunshine intensity weakened in late stage, it wasn’t favorable for accumulation of photosynthetic product, and the normal implementing of grouting process was affected. The measures should be used to prevent and control green-dry yield reduction of wheat, such as discharging water and preventing flood, breeding good seed, scientific planting and reasonable irrigation. [Conclusion] The research provided scientific basis for studying variety improvement, scientific plantation, reasonable irrigation, good quality and high yield of wheat.展开更多
基金supported by the National Key Research and Development Program of China (2016YFD0300403)the National Natural Science Foundation of China(31801298)the Fund of Shandong‘Double Top’Program,China (SYL2017YSTD05)。
文摘Delayed sowing mitigates lodging in wheat. However, the mechanism underlying the enhanced lodging resistance in wheat has yet to be fully elucidated. Field experiments were conducted to investigate the effects of sowing date on lignin and cellulose metabolism, stem morphological characteristics, lodging resistance, and grain yield. Seeds of Tainong 18,a winter wheat variety, were sown on October 8(normal sowing) and October 22(late sowing) during both of the 2015–2016 and 2016–2017 growing seasons. The results showed that late sowing enhanced the lodging resistance of wheat by improving the biosynthesis and accumulation of lignin and cellulose. Under late sowing, the expression levels of key genes(Ta PAL, Ta CCR, Ta COMT, TaCAD, and TaCesA1, 3, 4, 7, and 8) and enzyme activities(TaPAL and TaCAD) related to lignin and cellulose biosynthesis peaked 4–12 days earlier, and except for the TaPAL, TaCCR, and TaCesA1 genes and TaPAL, in most cases they were significantly higher than under normal sowing. As a result, lignin and cellulose accumulated quickly during the stem elongation stage. The mean and maximum accumulation rates of lignin and cellulose increased, the maximum accumulation contents of lignin and cellulose were higher, and the cellulose accumulation duration was prolonged. Consequently, the lignin/cellulose ratio and lignin content were increased from 0 day and the cellulose content was increased from 11 days after jointing onward. Our main finding is that the improved biosynthesis and accumulation of lignin and cellulose were responsible for increasing the stem-filling degree, breaking strength, and lodging resistance. The major functional genes enhancing lodging resistance in wheat that are induced by delayed sowing need to be determined.
基金This work was part of the projects of 40201028,30070429,49890330 and 30230230 supported by the National Natural Science Foundation of China(NFSC)the project of G1999011707 supported by National Key Basic Research Special Funds(NKBRSF).The authors would like to take the opportunity to thank the NFSC and the NKBRSF for their kindness of supporting these projects.
文摘Ammonia volatilized from aboveground parts of winter wheat was collected with an enclosure growth chamber and measured from jointing to maturing stage. The results showed that ammonia released from unfertilized plants grown in high and low fertility soils remained at low rates of 2.3 and 0. 9μg NH3 40 plant-1 h-1 respectively at late filling stage. However, fertilized plants rapidly increased the rates to 43. 4 and 52. 2μg NH3, 40 plant-1 h-1 in the high and low fertility soils, respectively, at the same period. The released a-mount was different in different parts of plants. At filling stage, lower senescing stems and leaves volatilized more ammonia than upper parts, i. e. , ears and flag leaves that grew normally, with an average of 1. 4 and 0.7μg NH3 20 plant-1 h-1 respectively, strongly suggesting that it was the senile organs that released large amounts of ammonia. At the grain filling stage, shortage of water supply (drought stress) reduced ammonia volatilization. The average rate of ammonia released under water stress was 0. 9μg NH3 40 plant-1 h-1, but 1.2μg NH3 40 plant-1 h-1 with moderate water supply. Application of N together with P fertilizer resulted in a higher ammonia volatilization than N fertilization alone at the maturing stage. The average rate released was 135.3 μg NH3 40 plant-1 h-1 when 0.4 g N and 0.13 g P had been added to per kg soil, while 33. 7μg when 0. 4 g N added alone. Ammonia volatilization from plants was closely related with plant biomass and N uptake;P fertilization increased plant biomass and N uptake and therefore increased its release.
基金supported by the National Key Research and Development Program of China (2017YFD0300408)the Major Research Projects of Anhui (202003b06020021)the Graduate Innovation Fund of Anhui Agricultural University (2020 ysj-5)。
文摘Late spring cold(LSC) occurred in the reproductive period of wheat impairs spike and floret differentiation during the reproductive period,when young spikelets are very cold-sensitive.However,under LSC,the responses of wheat spikelets at various positions,leaves,and stems and the interactions between them at physiological levels remain unclear.In the present study,two-year treatments at terminal spikelet stage under two temperatures(2 C,-2 C) and durations(1,2,and 3 days) were imposed in an artificial climate chamber to compare the effects of LSC on grain number and yield in the wheat cultivars Yannong 19(YN19,cold-tolerant) and Xinmai 26(XM26,cold-sensitive).The night temperature regimes were designed to reproduce natural temperature variation.LSC delayed plant growth and inhibited spike and floret differentiation,leading to high yield losses in both cultivars.LSC reduced dry matter accumulation(DMA,g) in spikes,stems,and leaves,reducing the DMA ratios of the spike to leaf and spike to stem.Plant cell wall invertase(CWINV) activity increased in upper and basal spikelets in YN19,whereas CWINV increased in middle spikelets in XM26.Under LSC,soluble sugar and glucose were transported and distributed mainly in upper and basal spikelets for glume and rachis development,so that spike development was relatively complete in YN19,whereas the upper and basal spikelets were severely damaged and most of the glumes in middle spikelets were relatively completely developed in XM26,resulting in pollen abortion mainly in upper and basal spikelets.The development of glumes and rachides was influenced and grain number per spike was decreased after LSC,with kernels present mainly in middle spikelets.Overall,reduced total DMA and dry matter partitioning to spikes under LSC results in poor spikelet development,leading to high losses of grain yield.
文摘[Objective] The research aimed to study influence of meteorological factor in late growth stage of wheat. [Method] Based on precipitation, sunshine and yield per unit of wheat in Anyang City in May of 1979-2008, the positive and negative influences of meteorological condition in late growth stage of wheat (May) on wheat yield in Anyang City were analyzed by using agricultural climatic statistical method. Moreover, the reason and defense measure of green-dry hazard in late growth stage of wheat in the city were studied. [Result] When the sunshine percentage in May > 55%, and rainfall < 45 mm, the wheat yield generally increased. But when it was overcast and rainy, and the sunshine was less, especially monthly rainfall > 80 mm, and monthly sunshine percentage < 55%, the wheat yield generally reduced. The overcast and rainy weather, flood in late growth stage of wheat were easy to cause green-dry yield reduction. The rainless weather even drought weren’t obviously unfavorable for good harvest of wheat. In May, when precipitation was too more, or duration was too long, and the air humidity was too big, the normal water supply and inorganic nutrient transmission were affected. Meanwhile, when the overcast and rainy weather was longer, the sunshine was shorter, and the sunshine intensity weakened in late stage, it wasn’t favorable for accumulation of photosynthetic product, and the normal implementing of grouting process was affected. The measures should be used to prevent and control green-dry yield reduction of wheat, such as discharging water and preventing flood, breeding good seed, scientific planting and reasonable irrigation. [Conclusion] The research provided scientific basis for studying variety improvement, scientific plantation, reasonable irrigation, good quality and high yield of wheat.
