The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity ...The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity （TAA）,superoxide anion radical scavenging activity （SARSA）,active oxygen scavenging activity （AOSA）,and DPPH （1,1-diphenyl-2 picrylhydrazyl free radical） radical scavenging activity.The results showed that the pigment had higher antioxidant activity and TAA,SARSA,AOSA and DPPH.scavenging activities at a certain concentration than Vc （antiscorbutic vitamin,vitamin C）,and the capacity increased with the increase of pigment concentration.Its TAA was 51.06 U mL-1,1.73 times of Vc,and SARSA 18 025.21 U mL-1,2.26% higher than Vc,and AOSA 3 776.31 U mL-1,1.24 times of Vc.As to the DPPH.scavenging activity of the pigment,there was a trend that higher concentration performed higher activity significantly improved with the company of Vc.The pigment showed significant antioxidant activities evaluated by different assays.Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.展开更多
为使黑小麦麸皮在全谷物功能性食品的研发中得到充分利用,对基于超声辅助法提取后的黑小麦麸皮中的多酚粗提物进行纯化,优化了大孔树脂纯化工艺,采用液相色谱与质谱联用(LC-MS)技术,对纯化后的麸皮多酚组成做了初步分析。结果显示:当样...为使黑小麦麸皮在全谷物功能性食品的研发中得到充分利用,对基于超声辅助法提取后的黑小麦麸皮中的多酚粗提物进行纯化,优化了大孔树脂纯化工艺,采用液相色谱与质谱联用(LC-MS)技术,对纯化后的麸皮多酚组成做了初步分析。结果显示:当样品溶液浓度为1.20 mg/m L、洗脱溶剂浓度为60%、进样流速为1.50 m L/min、洗脱速度为1.50 m L/min时,纯化效果较佳。纯化前后的黑小麦麸皮多酚纯度分别为2.60%±0.28%和14.27%±0.13%,纯化后多酚纯度约为纯化前的5.48倍。推测出纯化后的黑小麦麸皮多酚提取物中可能含有的九种多酚类物质。综上所述,大孔树脂纯化工艺有效地纯化了黑小麦麸皮多酚粗提物,一定程度上保持了多酚类物质的多样性。展开更多
基金supported by the National Technologies R&D Program of China (2006BAD01A02)the Excellent Medium-Youth Scientist Scientific Research Reward Fundation of Shandong Province, China (BS2009NY036)the Youth Science and Technology Creative Fundation Item of Shandong Agricultural University, China
文摘The anthocyanin pigment extracted from green-wheat-bran was studied to identify its antioxidant activity.The antioxidant activities of the pigment were evaluated by anti-lipid peroxidation,total antioxidant activity （TAA）,superoxide anion radical scavenging activity （SARSA）,active oxygen scavenging activity （AOSA）,and DPPH （1,1-diphenyl-2 picrylhydrazyl free radical） radical scavenging activity.The results showed that the pigment had higher antioxidant activity and TAA,SARSA,AOSA and DPPH.scavenging activities at a certain concentration than Vc （antiscorbutic vitamin,vitamin C）,and the capacity increased with the increase of pigment concentration.Its TAA was 51.06 U mL-1,1.73 times of Vc,and SARSA 18 025.21 U mL-1,2.26% higher than Vc,and AOSA 3 776.31 U mL-1,1.24 times of Vc.As to the DPPH.scavenging activity of the pigment,there was a trend that higher concentration performed higher activity significantly improved with the company of Vc.The pigment showed significant antioxidant activities evaluated by different assays.Results will provide a better understanding on antioxidant activity of green wheat and allow the screening or breeding of green wheat varieties with higher antioxidant activity for food processing.
文摘为使黑小麦麸皮在全谷物功能性食品的研发中得到充分利用,对基于超声辅助法提取后的黑小麦麸皮中的多酚粗提物进行纯化,优化了大孔树脂纯化工艺,采用液相色谱与质谱联用(LC-MS)技术,对纯化后的麸皮多酚组成做了初步分析。结果显示:当样品溶液浓度为1.20 mg/m L、洗脱溶剂浓度为60%、进样流速为1.50 m L/min、洗脱速度为1.50 m L/min时,纯化效果较佳。纯化前后的黑小麦麸皮多酚纯度分别为2.60%±0.28%和14.27%±0.13%,纯化后多酚纯度约为纯化前的5.48倍。推测出纯化后的黑小麦麸皮多酚提取物中可能含有的九种多酚类物质。综上所述,大孔树脂纯化工艺有效地纯化了黑小麦麸皮多酚粗提物,一定程度上保持了多酚类物质的多样性。
