Monitoring the changes in plasm C-reactive protein,fibrinogen and blood white cell in patients with primary hypertension combined with acute cerebral infarction

BACKGROUND： Inflammatory reaction and the increased level of its accompanying active protein play an important role in the occurrence and development of cerebral infarction. C-reactive protein, fibrinogen and white blood cell, as the monitoring index of inflammatory reaction, are very important in the occurrence and development of acute cerebral infarction. OBJECTIVE： To make a comparison between patients with primary hypertension accompanied with acute cerebral infarction and with simple primary hypertension by observing the changes in plasma C-reactive protein and fibrinogen levels as well as white blood cell and differential counts and analyzing their significances. DESIGN ： Controlled observation SETTING ： Ward Building for VIP, Shenzhen Hospital, Peking University. PARTICIPANTS： Totally 133 patients with primary hypertension were selected from Ward Building for VIP, Shenzhen Hospital, Peking University during September 2003 to September 2005, The diagnostic criteria were based on the hypertension diagnosis criteria formulated by the 7^th World Health Organization-International Society of Hypertension Guidelines （WHO-ISH） in 1998. The informed consents were obtained from all the participants. The involved patients were assigned into two groups： primary hypertension group, in which, there were 65 patients with primary hypertension （ degree 2）, including 42 males and 23 females, with mean age of （61 ±14）years and mean blood pressure of （162.7±6.8）/（94.2±8.4） mm Hg （1 mm Hg =0.133 kPa）, and primary hypertension combined with cerebral infarction group, in which, there were 68 patients with primary hypertension combined with cerebral infarction （ meeting the diagnostic criteria formulated in the 4^th National Cerebrovascular Diseases Meeting in 1995 and diagnosed by skull CT or MRI to exclude the patients with lacunar infarction）, including 42 males and 26 females, with mean age of （56±15） years and mean blood pressure of （176.4±9.2）/（96.3±9.7） mm Hg. METHODS： Plasm C-reactive protein and fibrinogen levels, and white blood cell and differential counts of patients in the two groups were examined 24 hours after stroke. The above indexes were re-examined in the primary hypertension combined with cerebral infarction group 72 hours after stroke. White blood cell and differential counts were performed with laser method （East Asia FE-95001 RAM-1, Japan）. The level of C-reactive protein was measured with turbidimetry （BNII Automatic Systems For Analysis, USA）. The level of fibrinogen was measured with algorithm method when prothrombin time was normal and with Clauss method when prothrombin time was abnormal （ACL Automatic Coagulation Analyzer, USA）. MAIN OUTCOME MEASURES： The plasm C-reactive protein and flbrinogen levels, and white blood cell and differential counts 24 hours after stroke in two groups and 72 hours after stroke in primary hypertension combined with cerebral infarction group. RESULTS： All the 133 involved patients participated in the result analysis. The plasm C-reactive protein and fibrinogen levels, and white blood cell and neutrophil counts in patients with primary hypertension were all within the normal range. The plasm C-reactive protein and fibrinogen levels, and white blood cell and neu- trophil counts in patients with primary hypertension combined with cerebral infarction were significantly higher than those in patients with primary hypertension 24 hours after stroke and 72 hours after stroke respectively[24 hours after stroke：（32.12±11.76） mg/L vs. （5.02±3.21 ） mg/L;（4.64±0.75） g/L vs. （3.12±0.49） g/L; （9.32±81）×10^9 L^- 1 vs. （5.78±1.32）×10^9L^- 1 （7.85±2.38）×10^9 L^- 1 vs.（3.49±1.28）×10^9 L^-1,t =7.094, 5.759,4.106,5.491, respectively,all P〈 0.01; 72 hours after stroke： （47.62±18.43） mg/L vs. （32.12±11.76） mg/L; （5.08±0.82） g/L vs. （4.64±0.75） g/L, t =2.864,2.220, respectively, both P 〈 0.05]. CONCLUSION： The increase in fibrinogen level and white blood cell count are the important index in monitoring primary hypertension combined with acute cerebral infarction. The increase in plasm C-reactive protein and fibrinogen levels 72 hours after stroke indicates that plasma C-reactive protein and fibrinogen are very important in the development of disease.

Research Progress of Combined Detection of WBC, CRP and SAA in Early Diagnosis of Respiratory Tract Infection in Children

Objective: To investigate the application of WBC, CRP and SAA combined detection in the early diagnosis of respiratory tract infection in children. Methods: Collect the literature reports on the early diagnosis of respiratory tract infection in children by the combined detection of WBC, CRP and SAA in recent years, and follow up the relevant literature reports from the selection of “new three routine” laboratory items for rapid diagnosis in pediatric outpatient department and the application of the combined detection of WBC, CRP and SAA in the early diagnosis of respiratory tract infection in children. Results: Many literature studies found that the combined detection of WBC, CRP and SAA has important clinical significance in the early diagnosis of respiratory tract infection in children. Conclusion: Through reviewing the relevant literature, we can understand the application of WBC, CRP and SAA combined detection in the early diagnosis of respiratory tract infection in children. To provide more accurate and reliable laboratory data for the early diagnosis and treatment of respiratory tract infection in children in the future.

High Sensitivity C-Reactive Protein Associated with Different Health Predictors in Middle-Aged and oldest old Chinese

Objective To describe the distribution of plasma high sensitivity C-reactive protein （hsCRP） and explore the relationship between hsCRP and metabolic risk factors among residents living in longevity areas of China. Methods 268 individuals aged between 40 and 59 years and 506 individuals aged over 90 years were selected from 5 longevity areas of China to participate in a cross section longitudinal cohort study. The participants were interviewed with general health related questionnaire to collect their demographic, behavioral and lifestyle data, as well as their chronic conditions, and meanwhile their physical and biomedical parameters including waist circumference （WC）, blood pressure （BP）, hsCRP, plasma lipids, and fasting blood glucose （FBG） were measured. Results The median of hsCRP was 0.99 mg/L in the middle-aged group and 2.76 mg/L in the oldest old group. No significant gender difference was observed between the above two groups. Among the oldest old individuals, 36.56% had an hsCRP level 〉3.0 mg/L. The prevalence of high hsCRP was 26.79% in the middle-aged group. The results of stepwise multiple linear regression analyses showed that HDL-C was independently associated with In （hsCRP） concentration in the middle-aged group, whereas In （TG）, HDL-C and FBG were correlated after adjustment for gender, study site, smoking, drinking, education and BMI in the oldest old group. Conclusion HDL-C is a stronger predictor of elevated hsCRP than other metabolic factors in the middle-aged population. For the oldest old persons, high TG, low HDL-C, and FBG predict elevated plasma hsCRP.

Heparin-binding protein combined with human serum albumin in early assessment of community-acquired pneumonia:A retrospective study

Objective:To investigate the application of heparin-binding protein along with albumin(HBP+ALB)in evaluating the severity of community-acquired pneumonia(CAP)and compares it with single HBP,white blood cells(WBCs),C-reactive protein(CRP),and procalcitonin(PCT).Methods:A total of 226 patients with CAP admitted to the Emergency Department of Zhujiang Hospital,Southern Medical University,Guangdong,China,between March 1,2021,and March 1,2022,were enrolled.The patients were grouped into two groups:mild CAP(n=175)and severe CAP(sCAP)(n=51).Patients'characteristics and laboratory data were obtained.ROC curve and the value of the area under the curve(AUC)were used to evaluate the predictive values of HBP,ALB,WBC,CRP,and PCT.Results:WBC count,CRP,PCT,HBP,creatinine,and D-dimer were higher in the sCAP group,while ALB was lower in the sCAP group(P<0.05)than those in the mild CAP group.The AUCs of WBC,CRP,PCT,HBP,and HBP+ALB were 0.633(95%CI:0.545-0.722,P<0.05),0.635(95%CI:0.542-0.729,P<0.05),0.705(95%CI:0.619-0.791,P<0.05),0.809(95%CI:0.736-0.883,P<0.05),and 0.889(95%CI:0.842-0.936,P<0.05),respectively.Conclusions:HBP+ALB has a higher predictive value than single HBP,PCT,CRP and WBC used alone for the early assessment of CAP.

血气分析检测、D-D及CRP水平用于PTE分型中的临床效果

目的探讨血气分析检测、血浆D-二聚体(D-D)和C反应蛋白(CRP)在血栓性肺栓塞分型中的应用效果。方法选取2021年9月至2022年7月该院74例血栓性肺栓塞患者,以栓塞范围为依据分为甲组(非大面积栓塞,37例)与乙组(大面积栓塞,37例),并选择同期37例健康体检者为对照组,检测3组受检者血气指标、D-D和CRP,分析检测结果。结果甲组及乙组氧分压比对照组低,而CRP及D-D较对照组高,差异有统计学意义(P<0.05);甲组二氧化碳分压比乙组及对照组低,差异有统计学意义(P<0.05);而乙组与对照组二氧化碳分压差异无统计学意义(P>0.05)。甲组氧分压阳性检出率比乙组低,而二氧化碳分压、D-D阳性检出率比乙组高,差异有统计学意义(P<0.05);甲组与乙组CRP阳性检出率差异无统计学意义(P>0.05)。结论血浆D-D、血气分析检测及CRP在血栓性肺栓塞分型诊断中具有较高的应用价值,可为疾病诊断及治疗提供参考,值得临床采纳、推广。

血清PCT、CRP检测联合血液细菌培养对菌血症的早期诊断价值研究

目的探讨血清降钙素原(PCT)、C反应蛋白(CRP)检测联合血液细菌培养(血培养)对菌血症的早期诊断价值。方法将2021年1月至2022年12月广州医科大学附属第四医院收治的285例疑似菌血症患者作为研究对象。患者均进行血清PCT、CRP检测及血培养。根据血培养结果分为菌血症组、非菌血症组,菌血症组又分为革兰阳性菌组、革兰阴性菌组。分析3项指标联合检测对菌血症的诊断价值;同时观察患者病情控制前后血清指标变化。结果菌血症组血清PCT和CRP水平明显高于非菌血症组(P<0.05);革兰阴性菌组患者PCT、CRP水平均明显高于革兰阳性菌组(P<0.05);菌血症患者PCT、CRP联合血培养阳性率为99.35%,高于单项检测,差异有统计学意义(P<0.05)。患者病情控制前后血清PCT、CRP水平比较,差异有统计学意义(P<0.05)。结论血清PCT、CRP检测联合血培养对菌血症的早期诊断具有一定价值,有助于及时调整抗菌药物治疗方案,改善预后。

脑梗死患者血清同型半胱氨酸、超敏C反应蛋白、血脂检测结果分析

目的探讨血清同型半胱氨酸(homocysteine,Hcy)、超敏C反应蛋白(hypersensitive C-reactive protein,hs-CRP)、血脂指标检测对脑梗死患者的诊断效能。方法选择2020年5月—2023年6月本院收治的127例脑梗死患者设为观察组;选择同期入院体检的127例健康体检者设为对照组。观察两组受检者Hcy、hs-CRP以及血脂水平。结果观察组Hcy、hs-CRP、TC、TG、LDL-C均高于对照组,且HDL-C低于对照组(P<0.05);观察组患者中,脑栓塞患者Hcy、hs-CRP、TC、TG、LDL-C水平均高于脑血栓形成和腔隙性脑梗死患者,且HDL-C低于脑血栓形成和腔隙性脑梗死患者(P<0.05)。结论血清Hcy、hs-CRP以及血脂水平与脑梗死的发生和发展密切相关,通过多种途径参与并影响脑梗死形成,将联合检测应用于脑梗死患者早期诊断过程中,能为后续治疗和预后判断提供有效参考。

Role of plasma C-reactive protein and white blood cell count in predicting in-hospital clinical events of acute type A aortic dissection

Background A few recent studies have reported that inflammation is associated with the prognosis of acute aortic dissection （AD）. There is, however, no systemic investigation regarding the role of plasma C-reactive protein （CRP） and white blood cell （WBC） levels in predicting in-hospital clinical events of acute type AAD. Methods The levels of high-sensitivity CRP and WBC counts were systemically determined after admission in 36 patients with acute type A AD. The variations of plasma CRP and WBC levels in different time windows （admission, 1, 2, 3, 4, 6, 8 days） in patients with acute type AAD were analyzed between patients with events and without events. Results During hospitalization, five patients died, and increased levels of CRP and WBC were found in patients died with acute type A AD compared with patients survived （P 〈0.01, respectively）. Medical treatment may significantly decrease inflammatory response in survived patients with acute type A AD. Additionally, patients with complication of pleural effusion showed higher CRP and WBC levers （P=0.046, P=-0.018, respectively）. Lower WBC levels were found in survived patients treated medically （P=-0.001）. Moreover, mean CRP and WBC levels had positive correlations with aortic diameter （r=0.364, P--0.000; r=0.333, P=0.000, respectively） and age （r=0.270, P=0.000, respectively）, while negative correlations with the time from onset of symptoms to hospital admission （r= -0.229, P=0.000, r= -0.200, P=0.002, respectively）. Univariate analysis showed that age 〉65 years, CRP zl 2.05 rag/L, WBC 〉12.16×10^9/L, aortic diameter 〉48 mm, pleural effusion and diastolic blood pressure 〉105 mmHg were associated with hospital mortality. While CRP 〉12.05 mg/L, WBC ≥12.16×10^9/L, aortic diameter 〉48 mm were strongly associated with hospital mortality in multiple Logistic regression analysis. Conclusions The results suggested that CRP and WBC were preferred markers for predicting the clinical events in patients with acute type A AD, especially death during hospitalization. Therefore, further study enrolling larger cohort, prospective study would be warranted.

Effects of Suhuang Zhike Capsule on Pulmonary Function, Blood Gas Analysis Index, Serum PCT and CRP Expression in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease

OBJECTIVE: To observe the effects of Suhuang Zhike Capsule on pulmonary function, blood gas analysis index, serum PCT and CRP expression in patients with acute exacerbation of chronic obstructive pulmonary disease. METHODS: A total of 88 patients with acute exacerbation of chronic obstructive pulmonary disease admitted to the Department of Respiratory Medicine, Xuancheng People's Hospital of Anhui Province from December 2014 to December 2016 were randomly divided into the observation group and the control group, with 44 cases in each group. The control group was given routine clinical treatment, and the observation group was given Suhuang Zhike Capsule on the basis of the treatment method of the control group. After 7 days' treatment, the improvement of lung function indexes were observed and evaluated before and after treatment(forced expiratory volume in one second(FEV_1), forced expiratory volume occupancy in the 1^(st) second percentage of vital capacity(FEVl/FVC), peak expiatory flow(PEF)), blood gas analysis index(Arterial oxygen partial pressure(PaO_2) and arterial blood carbon dioxide partial pressure(PaCO_2), oxygenation index(OI)) and serum cytokine levels(procalcitonin(PCT) and C reactive protein(CRP). RESULTS: The total effective rate of the observation group was significantly higher than that of the control group(P < 0.05). The FEV_1, FEV_1/FVC and PEF in 2 groups were significantly increased after the treatment(P < 0.05), and the above lung function indexes in the observation group were significantly higher than the control group(P < 0.05). PaO_2 and OI were significantly increased after the treatment(P < 0.05), PaCO_2 was significantly decreased after the treatment(P < 0.05), and the improvement of above blood gas analysis indexes were significantly superior of the observation group than the control group(P < 0.05). The serum PCT and CRP levels in 2 groups were significantly decreased after the treatment(P < 0.05), and the improvement in the observation group were more significant than that in the control group(P < 0.05). CONCLUSION: Suhuang Zhike Capsule can inhibit serum inflammatory cytokine levels in patients with acute exacerbation of chronic obstructive pulmonary disease, improve blood gas analysis indicators, and improve lung functions.

Study on the relationship between syndrome characteristics degree and inflammatory factor and negative emotional scale in"double heart disease"patients with Qi stagnation blood stasis and heart gallbladder heat stagnation

Objective:To Discuss the correlation between Hypersensitivity C-reactive Protein(Hs-CRP),Total Cholesterol(TC),Triglyceride(TG),negative emotion scale and TCM syndrome scores in“Double Heart Disease”patients with Qi stagnation blood stasis and heart gallbladder heat stagnation.Method:Fifty-two patients in Western Medicine Diagnosis of Double Heart Disease,in TCM syndrome identified as Qi stagnation blood stasis Heart gallbladder heat stagnation syndrome,detects it Hs-CRP,TC,TG levels by ELISA,use TCM Syndromes Scale to evaluate TCM Syndrome,use the Pittsburgh Sleep Quality Index(PSQI)and Self-rating symptom scale(SCL-90)to assess anxiety and sleep levels,analyze the correlation between TCM syndrome scores and Hs-CRP,TC,TG level,PSQI index,SCL-90 index.Result:There was a significant positive correlation between Hs-CRP,TG level and TCM Syndrome scores(P<0.05);TC level was postively correlated with TCM Syndrome scores,but there was no statistical significance(P>0.05);There was a significant positive correlation between PSQI index,SCL-90 index and TCM Syndrome scores(P<0.05).Conclusion:In“Double Heart Disease”patients with Qi stagnation blood stasis and heart gallbladder heat stagnation,there have characteristic syndrome changes in terms of inflammatory factor level,blood lipid level and negative emotion score;Which the above indexes can reflect the severity of TCM syndromes to a certain extent and provide the basis for the effective intervention treatment of TCM.

Nano-seq宏基因组测序在关节置换术后假体周围感染诊断中的应用价值

目的:探讨Nano-seq宏基因组测序在关节置换术后假体周围感染诊断中的应用价值。方法:纳入38例人工关节置换术后因疑似假体周围感染再次住院的患者。患者住院后,抽血测定血清C反应蛋白(C-reactive protein,CRP)含量、血沉(erythrocyte sedimentation rate,ESR)和血浆纤维蛋白原(fibrinogen,FIB)含量等指标,抽取关节液进行病原微生物培养和Nano-seq宏基因组测序。患者出院后,从病历系统中提取患者住院时测定的血清CRP含量、ESR、血浆FIB含量及病原微生物培养检验报告、Nano-seq宏基因组测序检验报告及出院诊断结果。分别根据血清CRP含量、ESR、血浆FIB含量、病原微生物培养检验报告、Nano-seq宏基因组测序检验报告诊断假体周围感染,并以出院诊断结果为金标准,计算上述指标诊断假体周围感染的敏感度、特异度、准确度、阳性预测值、阴性预测值。采用受试者操作特征(receiver operating characteristic,ROC)曲线评价不同指标诊断假体周围感染的应用价值。结果:血清CRP含量诊断假体周围感染的敏感度为90.48%、特异度为41.18%、准确度为68.42%,ESR诊断假体周围感染的敏感度为66.67%、特异度为64.71%、准确度为65.79%,血浆FIB含量诊断假体周围感染的敏感度为66.67%、特异度为70.59%、准确度为68.42%,病原微生物培养诊断假体周围感染的敏感度为61.90%、特异度为94.12%、准确度为76.32%,Nano-seq宏基因组测序诊断假体周围感染的敏感度为95.24%、特异度为82.35%、准确度为89.47%。Nano-seq宏基因组测序诊断假体周围感染的敏感度高于ESR、血浆FIB含量、病原微生物培养(P=0.045,P=0.045,P=0.020),特异度高于血清CRP含量(P=0.032),准确度高于血清CRP含量、ESR、血浆FIB含量(P=0.047,P=0.026,P=0.047)。血清CRP含量、ESR、血浆FIB含量、病原微生物培养、Nano-seq宏基因组测序诊断假体周围感染的ROC曲线下面积分别为0.791(P=0.002)、0.706(P=0.031)、0.734(P=0.014)、0.780(P=0.000)、0.888(P=0.000)。结论:Nano-seq宏基因组测序在关节置换术后假体周围感染诊断中具有较高的应用价值。

Combined action of C-reactive protein and lipid profiles on risk of hypertension and prehypertension in Mongolian adults in Inner Mongolia, China

Background Many studies have suggested that C-reactive protein （CRP） and blood lipids are associated with hypertension and cardiovascular disease （CVD）.However,few studies discussed the combined action of CRP and blood lipids on the risk of hypertension and prehypertension.This study aimed to investigate the combined action of CRP and lipid profiles on the risk of hypertension and prehypertension in Mongolian adults from Inner Mongolia,China.Methods The systolic and diastolic blood pressure,height,weight and waist circumference were measured and factors such as smoking,alcohol intake,family history of hypertension,etc.,were investigated and CRP,low-density lipoprotein cholesterol （LDL-C）,triglycerides （TG） were tested for 2 534 Mongolian adults aged ≥20 years.The subjects were divided into four subgroups,namely CRP 〈median and LDL-C （TG） 〈median subgroup,CRP 〈median and LDL-C （TG） 〉median subgroup,CRP 〉median and LDL-C （TG） 〈median subgroup and CRP 〉median and LDL-C （TG） 〉median subgroup.The ORs （95% C/s） of hypertension and prehypertension for the subgroups were calculated by univariate and multivariate analysis.Results The multivariate adjusted ORs （95%CIs） of hypertension/prehypertension were 1.389 （0.979-1.970）/1.1 51（0.865-1.531）,1.666 （1.159-2.394）/1.431 （1.060-1.930）,1.756 （1.242-2.484）/ 1.770 （1.321-2.372）,for CRP 〈median and LDL-C 〉median subgroup,CRP 〉median and LDL-C 〈median subgroup,and CRP 〉median and LDL-C 〉median subgroup,respectively,compared with CRP 〈median and LDL-C 〈median subgroup.Similarly,the multivariate adjusted ORs （95% CIs） of hypertension/prehypertension were 2.032 （1.394-2.963）/1.442 （1.047-1.988）,1.412 （0.960-2.079）/1.596 （1.166-2.184）,and 2.197 （1.595-3.027）/1.730 （1.321-2.266） for CRP 〈median and TG 〉median subgroup,CRP 〉median and TG 〈median subgroup,and CRP 〉median and TG 〉median subgroup,respectively,compared with CRP 〈median and TG 〈median subgroup.The risks of hypertension and prehypertension were the highest in the CRP 〉median and LDL-C （TG） 〉median subgroup among the four subgroups.Conclusions Subjects with both CRP 〉median and LDL-C （TG） 〉median had highest risks of hypertension and prehypertension among all subjects.This study appeared to indicate that the combined action of elevated CRP and elevated LDL-C （TG） further increase the risks of hypertension and prehypertension among Mongolian population.

血常规联合C反应蛋白用于儿童上呼吸道感染诊断的检测价值

目的探讨血常规与C反应蛋白(C-reactive protein,CRP)联合诊断儿童上呼吸道感染的临床诊断价值。方法本次研究对象为徐州市矿山医院检验科2021年1月—2023年1月收治的83例上呼吸道感染患儿,依据疾病类型分组,其中40例为细菌感染,43例为病毒或支原体等非细菌感染;另选取同期于徐州市矿山医院体检的40名健康儿童设为对照组,检测与比较三组血常规与CRP水平。结果与健康组相比,细菌感染组与非细菌感染组中性粒细胞(neutrophil,NEU)与白细胞计数(white blood cell count,WBC)及CRP水平均更高(P<0.05),且细菌感染组上述指标水平高于非细菌感染组(P<0.001);血常规联合CRP诊断儿童上呼吸道感染的灵敏度、阳性与阴性预测值及诊断符合率均高于血常规单独检测(P<0.05),亦高于CRP单独检测,但比较差异无统计学意义(P>0.05)。结论血常规联合CRP诊断儿童上呼吸道感染的检测价值较高,可提高诊断符合率,便于指导临床合理用药。

血培养联合血清PCT、hs-CRP水平检测用于早期诊断细菌性血流感染的价值

目的 探讨细菌性血流感染早期诊断中血培养联合血清降钙素原(PCT)、超敏C反应蛋白(hs-CRP)水平检测的应用价值。方法 选取2019年6月至2022年6月在河南中医药大学人民医院接受治疗的58例细菌性血流感染患者,将其命名为感染组(N=58),另选取58名健康体检者,将其命名为对照组(N=58),比较不同组别、不同细菌种类的PCT、hs-CRP水平,分析不同检测方式的诊断价值。结果 感染组PCT、hs-CRP水平较对照组更高,差异有统计学意义(P<0.001);感染革兰氏阳性菌患者PCT水平较感染革兰氏阴性菌患者更低,差异有统计学意义(P<0.001);血培养联合血清PCT、hs-CRP水平检测的灵敏度、特异度、阳性预测值与阴性预测值比单一检测方式相更高,差异有统计学意义(P<0.001)。结论 血培养联合血清PCT、hs-CRP水平检测在早期诊断细菌性血流感染中的应用能够提高检出率,更有利于后续治疗。

糖化血红蛋白、空腹血糖以及24h尿总蛋白与糖尿病肾病早期肾损伤的相关性

目的观察糖尿病肾病患者空腹血糖、糖化血红蛋白检测及其24h尿总蛋白的相关性研究。方法选取2021年7月—2023年10月本院接收的糖尿病肾病患者60例作为实验组,其中30例合并肾病作为糖尿病肾病组,30例作为非肾病组,另选取健康人60例作为对照组,三组均进行糖化血红蛋白、空腹血糖、24h尿总蛋白的检测,对比组间及组内检测结果情况。结果实验组糖化血红蛋白、空腹血糖、24h尿总蛋白水平均高于对照组(t=15.150,88.610,13.350;P<0.05);肾病组糖化血红蛋白、空腹血糖、24h尿总蛋白水平均高于非肾病组(t=10.400,7.495,21.930;P<0.05);患者体内糖化血红蛋白水平≥9.0%时糖尿病肾病的检出率高于<9.0%(χ^(2)=9.320,P<0.05);患者体内空腹血糖水平≥11.1 mmol·L^(-1)时糖尿病肾病的检出率高于<11.1 mmol·L^(-1)(χ^(2)=9.320,P<0.05)。结论糖化血红蛋白、空腹血糖、24h尿总蛋白的含量与糖尿病肾病的严重程度呈正相关关系,其诊断效果显著,安全性好,应多加推广应用。

尿红细胞形态学联合尿蛋白定量检测在肾病诊断中的应用研究

目的 探讨尿红细胞形态学联合尿蛋白定量检测在肾病诊断中的临床价值。方法 选取2020年5月至2022年9月我院收治的86例疑似肾病患者,均进行尿红细胞形态学、尿蛋白定量检测及肾活检穿刺,并以肾活检穿刺为金标准,分析不同诊断方法对肾病的诊断价值。结果 86例疑似患者经肾活检穿刺检出75例肾病,其中尿红细胞形态学检出59例,尿蛋白定量检出63例,联合检测检出73例;联合检测诊断肾病的灵敏度、准确度、阴性预测值均高于尿红细胞形态学、尿蛋白定量检测,差异有统计学意义(P<0.05)。结论 尿红细胞形态学联合尿蛋白定量检测可提高肾病的诊断效能,有助于指导临床早期治疗。

CRP联合WBC及NEUT检测在儿童支气管感染性疾病中的应用价值

目的探究C-反应蛋白(C-reactive protein,CRP)联合白细胞(white blood cell,WBC)及中性粒细胞(neu‐trophil,NEUT)检测在儿童支气管感染性疾病中的应用价值。方法选取2022年9月—2023年5月南通苏通科技产业园区江海医院(南通大学附属医院苏通分院)收治的591例支气管感染性疾病患儿及240例健康体检儿童作为研究对象,分为细菌感染组(n=591)及健康对照组(n=240)。两组儿童均采取CRP及血常规检测,对比分析两组儿童CRP、WBC及NEUT的检出值。结果细菌感染组CRP、WBC、NEUT水平均高于健康对照组,差异有统计学意义(t=110.333、28.565、25.394,P<0.05);轻度细菌感染组CRP、WBC、NEUT水平低于重度细菌感染组,差异有统计学意义(t=80.442、13.749、9.382,P<0.05)。结论CRP联合WBC、NEUT水平检测能够为支气管感染性疾病患儿提供较为准确的诊断和治疗依据。

迈瑞BC-5390全自动血液分析仪检测C反应蛋白的性能研究

目的 ：评价迈瑞BC-5390全自动血液分析仪（以下简称“BC-5390血液分析仪”）检测C反应蛋白的性能。方法：随机收集临床检测C反应蛋白的血标本100份,分别用BC-5390血液分析仪和贝克曼库尔特AU-5800全自动生化分析仪（以下简称“AU-5800生化分析仪”）进行检测,通过对2种方法所测得的结果进行相关性分析,评价BC-5390血液分析仪检测血标本中C反应蛋白的精密度、稳定性、线性范围、携带污染率,验证不同仪器检测结果相关性、仪器不同模式检测结果相关性及内源性干扰等。结果：BC-5390血液分析仪检测C反应蛋白的精密度、线性范围均在允许的范围内;冰箱及室温保存标本条件下48 h内C反应蛋白结果无显著变化,相对偏差均〈5.0%,线性范围良好（0.01~320 mg/L）,r=0.998;携带污染率为0.18%,符合仪器求;BC-5390血液分析仪测定C反应蛋白的结果与AU-5800生化分析仪相关性良好（r=0.999）;自动进样模式与手动进样模式检测C反应蛋白结果相关性良好（r=0.997）;内源性干扰实验除异常高值胆红素和白细胞外,其余未见异常干扰。结论：BC-5390血液分析仪测定C反应蛋白准确度高、重复性好,线性范围能满足临床所需,携带污染率极低,且用血量少、检测快速方便,与AU-5800生化分析仪相关性好,结果可靠,特别适用于临床上急诊及儿科标本的检测,是临床快速测定C反应蛋白的理想分析仪器。

不孕相关精子蛋白抗体检测的临床意义

目的:分析不孕相关精子蛋白抗体与免疫性不孕的关系。方法:收集临床抗精子抗体阳性的不孕患者的外周血标本,ELISA法检测STP-4、TSA-1、SPAG7、FA-1及YLP-12与免疫性不孕相关的精子蛋白的抗体。结果:免疫性不孕相关精子蛋白的抗体阳性率仅有15%。结论:检测不孕相关精子蛋白的抗体对免疫性不孕的临床诊疗更为有益。

甲醛致人血淋巴细胞DNA-蛋白质交联作用的定量研究

目的 探讨甲醛致 DNA-蛋白质的交联作用以及交联的检测方法。方法 以人血淋巴细胞为材料 ,设定 0、0 .0 0 5、0 .0 2 5、0 .1 2 5、0 .6 2 5 mmol/ L 五个甲醛浓度梯度 ,采用 KCl- SDS沉淀法来检测染毒后细胞中 DNA-蛋白质交联的含量。结果 甲醛浓度在 0、0 .0 0 5、0 .0 2 5 mm ol/ L 时 ,交联现象不明显或不能诱导DPC(P>0 .0 5 ) ;在 0 .1 2 5、0 .6 2 5 mm ol/ L 时交联程度显著增加 (P<0 .0 1 ) ,并有较好的剂量 -效应关系。结论 在低浓度时 ,甲醛诱导 DNA-蛋白质交联现象不明显或不诱导该现象 ,在高浓度时可显著诱导交联现象。KCl- SDS沉淀法检测